Quality and antioxidant properties of a reduced-sugar pomegranate juice jelly with an aqueous extract of pomegranate peels

2013 ◽  
Vol 136 (1) ◽  
pp. 109-115 ◽  
Author(s):  
Janeth Ventura ◽  
Francisco Alarcón-Aguilar ◽  
Rubén Roman-Ramos ◽  
Efraín Campos-Sepulveda ◽  
Maria L. Reyes-Vega ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Pomegranate Juice

scholarly journals Evaluation of Toxicity and Antioxidant Property of Cassia fistula Stem Bark Extracts in Zebrafish

2020 ◽  
Vol 10 (03) ◽  
pp. 109-115
Author(s):  
Shreya Udaya ◽  
Nishith Babu ◽  
Dechamma Pandyanda Nanjappa ◽  
Krithika Kalladka ◽  
Gunimala Chakraborty ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Antioxidant Property ◽  
Stem Bark ◽  
Oxygen Species ◽  
Cassia Fistula ◽  
Potential Applications ◽  
Ethanol Extracts ◽  
Stress Models

Abstract Objective This study was aimed at evaluating the toxicity and the antioxidant property of Cassia fistula stem bark extracts in zebrafish. Materials and Methods Crude aqueous and ethanol extracts of C. fistula stem bark were obtained following a standard solvent-based extraction method. The toxicity of these extracts on zebrafish embryonic development was determined and the LC50 values were calculated. Finally, the antioxidant property of C. fistula stem bark extracts was determined in arsenic-induced oxidative stress models of zebrafish. Results The aqueous extract of C. fistula stem bark showed a slightly larger LC50 value (213.6 ppm) compared with the ethanol extract (LC50 = 63.5 ppm), suggesting a lower toxicity of the aqueous extract. A significant reduction of reactive oxygen species (ROS) signal was observed in arsenic-exposed embryos treated with the aqueous extract, but not the ethanol extract, indicating that the antioxidant activity is present only in the aqueous extract of C. fistula stem bark. Conclusion Identification of antioxidants from natural sources is desirable because of increasing safety concerns associated with synthetic antioxidants. This study demonstrated that aqueous extract from C. fistula stem bark possesses antioxidant properties, which can be further characterized for mechanism of action and potential applications.

Investigation of Antioxidant Activity of Pomegranate Juices by Means of Electron Paramagnetic Resonance and UV-Vis Spectroscopy

2015 ◽  
Vol 98 (4) ◽  
pp. 866-870 ◽  
Author(s):  
Violetta Kozik ◽  
Krystyna Jarzembek ◽  
Agnieszka Jędrzejowska ◽  
Andrzej Bąk ◽  
Justyna Polak ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Free Radical ◽  
Epr Spectroscopy ◽  
Antioxidant Properties ◽  
Free Radical Scavenger ◽  
Pomegranate Juice ◽  
Radical Scavenger ◽  
Paramagnetic Resonance ◽  
Vis Spectroscopy ◽  
Electron Paramagnetic

Abstract Pomegranate fruit (Punica granatum L.) is a source of numerous phenolic compounds, and it contains flavonoids such as anthocyanins, anthocyanidins, cyanidins, catechins and other complexes of flavonoids, ellagitannins, and hydrolyzed tannins. Pomegranate juice shows antioxidant, antiproliferative, and anti-atherosclerotic properties. The antioxidant capacity (TEAC) of the pomegranate juices was measured using electron paramagnetic resonance (EPR) spectroscopy and 1,1-diphenyl-2-picrylhydrazyl (DPPH•) as a source of free radicals, and the total phenolic (TP) content was measured using UV-Vis spectroscopy. All the examined pomegranate juices exhibited relatively high antioxidant properties. The TEAC values determined by means of EPR spectroscopy using Trolox (TE) as a free radical scavenger were in the range of 463.12 to 1911.91 μmol TE/100 mL juice. The TP content measured by the Folin-Ciocalteu method, using gallic acid (GA) as a free radical scavenger, widely varied in the investigated pomegranate juice samples and ranged from 1673.62 to 5263.87 mg GA/1 L juice. The strongest antioxidant properties were observed with the fresh pomegranate juices obtained from the fruits originating from Israel, Lebanon, and Azerbaijan. Correlation analysis of numerical data obtained by means of EPR spectroscopy (TEAC) and UV-Vis spectroscopy (TP) gave correlation coefficient (r) = 0.90 and determination coefficient (r2) = 0.81 (P <0.05).

Antidiarrheal, Antimicrobial, and Antioxidant Properties of the Aqueous Extract of Tunisian Persimmon (Diospyros kaki Thunb.) Fruits

2021 ◽  
Author(s):  
Nourhène Dhawefi ◽  
Saber Jedidi ◽  
Kais Rtibi ◽  
Mourad Jridi ◽  
Houcem Sammeri ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Diospyros Kaki

Strong antihemolytic and antioxidant properties of aqueous extract from Algerian Hammada elegans (Bge.) Botsch (Chenopodiaceae)

2021 ◽  
Vol 17 ◽  
Author(s):  
Brahim Asseli ◽  
Reguia Mahfoudi ◽  
Amar Djeridane ◽  
Mohamed Yousfi
Keyword(s):  
Free Radical ◽  
Aqueous Extract ◽  
Radical Scavenging Activity ◽  
Condensed Tannin ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Vitro Assay

Background: Research on medicinal plant antioxidants has emerged as a potential therapeutic to prevent free radical generated damage in the human body. Hammada elegans Botsch (popularly known as “Ajram”) is a xerophytic plant widely found in Laghouat region, but there are only a few reports about the biological or chemical properties of these species. Hence, the aim of this study is to investigate the antioxidant and the antihemolytic activities of hexanic, acetonic, methanolic and aqueous extracts of aerial parts of Algerian Hammada elegans Botsch by employing different in vitro assay systems. Methods: The total phenolic content, the flavonoid content and the condensed tannin amount were analyzed using Folin-Ciocalteu, aluminum chloride and vanillin assays, respectively. The in vitro antioxidant capacity of extracts was assessed by CUPRAC, iron chelating, ABTS•+and antihemolytic assays, and was expressed as EC50 values. Results: Among the analyzed extracts, the aqueous extract had the highest phenolic, flavonoid and tannin contents. Also, this extract displayed the highest antioxidant capacities compared to the other extracts and standards. Its EC50 value for ABTS radical-scavenging activity was 0.265 ± 0.003 mg/L. Moreover, this extract showed high iron (II) chelating ability (EC50 = 0.958 ± 0.001 mg/L), and good antioxidant activity in the cupric ion reducing activity (CUPRAC) in a concentration dependent manner (EC50 were 0.709 ± 0.002 mg/L). Additionally, this extract had the best antihemolytic activity against AAPH-induced hemolysis (EC50=0.090 ± 0.004 mg/L). Conclusion: Our study revealed that the aqueous extract of Hammada elegans Botsch, is a potential source of antioxidants which possess a high protective effect of membrane against free radical.

scholarly journals Phytochemical Screening and in vitro Antioxidant Properties of Methanol and Aqueous Leaf Extracts of Geophila obvallata

2018 ◽  
pp. 1-11
Author(s):  
Iserhienrhien Lucky Osafanme ◽  
Okolie Paulinus Ngozi
Keyword(s):  
Aqueous Extract ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Scientific Basis ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Reducing Ability

Aim: This study investigated the phytochemical constituents and in vitro antioxidant properties of methanol and aqueous leaf extracts of Geophila obvallata using standard methods. Materials and Methods: The in vitro antioxidant assays carried out were 1, 1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging ability, Nitric oxide (NO•) radical scavenging activity assay, 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS•+) radical cation scavenging assay, ferric reducing properties and hydroxyl radical scavenging assays. Results: Phytochemical analysis revealed the presence of alkaloids, flavonoids, phenolic compounds, steroids, saponins, terpernoids and cardiac glycosides in both extracts. Relative to the aqueous extract, the methanol extract contained a higher amount of the secondary metabolites. However, both extracts exhibited appreciable and dose-dependent capacities for quenching DPPH, ABTS•+ and NO• free radicals, and potent ferric reducing ability to levels comparable to those of ascorbic acid. The crude methanol extract showed significantly increased (P<0.05) antioxidant activity than the aqueous extract. Conclusion: It was concluded that the extract possesses strong antioxidant properties due to its content of phytochemicals, and provides scientific basis for its ethno medicinal applications.

scholarly journals Anti-leukemia properties of cadmium nanoparticles in the in vitro and in vivo condition: A chemobiological study

2021 ◽  
Author(s):  
Yudi Miao ◽  
Behnam Mahdavi ◽  
Mohammad Zangeneh
Keyword(s):  
Acute Myeloid Leukemia ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Myeloid Leukemia ◽  
Antioxidant Properties ◽  
Leukemia Cell ◽  
Sem Images ◽  
Acute Myeloid

IntroductionThe present study investigated the anti-acute myeloid leukemia effects of Ziziphora clinopodides Lam leaf aqueous extract conjugated cadmium nanoparticles.Material and methodsTo synthesize CdNPs, Z. clinopodides aqueous extract was mixed with Cd(NO3)2 .4H2O. The characterization of the biosynthesized cadmium nanoparticles was carried out using many various techniques such as UV-Vis. and FT-IR spectroscopy, XRD, FE-SEM, and EDS.ResultsThe uniform spherical morphology of NPs was proved by FE-SEM images with NPs the average size of 26.78cnm. For investigating the antioxidant properties of Cd(NO3)2, Z. clinopodides, CdNPs, and Daunorubicin, the DPPH test was used. The cadmium nanoparticles inhibited half of the DPPH molecules in a concentration of 196 µg/mL. To survey the cytotoxicity and anti-acute myeloid leukemia effects of Cd(NO3)2, Z. clinopodides, CdNPs, and Daunorubicin, MTT assay was used on the human acute myeloid leukemia cell lines i.e., Murine C1498, 32D-FLT3-ITD, and Human HL-60/vcr. The IC50 of the cadmium nanoparticles was 168, 205, and 210 µg/mL against Murine C1498, 32D-FLT3-ITD, and Human HL-60/vcr cell lines, respectively. In the part of in vivo study, DMBA was used for inducing acute myeloid leukemia in mice. CdNPs similar to daunorubicin ameliorated significantly (p≤0.01) the biochemical, inflammatory, RBC, WBC, platelet, stereological, histopathological, and cellular-molecular parameters compared to the other groups.ConclusionsAs mentioned, the cadmium nanoparticles had significant anti-acute myeloid leukemia effects. After approving the above results in the clinical trial studies, these cadmium nanoparticles can be used as a chemotherapeutic drug to treat acute myeloid leukemia in humans.

scholarly journals Allablanckia floribunda hypotensive activity on ethanol induced hypertension in rats

2018 ◽  
Vol 7 (2) ◽  
pp. 146-151
Author(s):  
Danielle Claude Bilanda ◽  
◽  
Paul Désiré Djomeni Dzeufiet ◽  
Orelien Mtopi Bopda ◽  
Pierre Kamtchouing ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Aqueous Extract ◽  
Plant Extract ◽  
Colorimetric Method ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Hdl Cholesterol ◽  
Hypotensive Activity ◽  
Hypertensive Rats ◽  
Induced Hypertension

Background: Chronic alcohol intake is related to hypertension. In the present work, we investigated the effect of Allablanckia floribunda Oliver (Clusiaceae) aqueous extract in alcohol-induced hypertensive rats and on related oxidative stress damages. Methods: Alcohol-induced hypertensive rats (AHR) was obtained by oral administration of ethanol (3 g/kg/day during 8 weeks). Blood pressure and heart rate were evaluated using the direct cannulation method. The effects of the extract on lipid profile as well as kidney and liver functions were studied. Free radical scavenging and antioxidant properties of the extract were evaluated by colorimetric method. The effects of A. floribunda were evaluated after 4 weeks of treatment with alcohol. Results: At the doses of 200 and 400 mg/kg/day, A. floribunda significantly decreased the mean blood pressure of AHR by 14.06 and 23.25 % respectively. Administration of the plant extract lead to the reduction of total cholesterol by 41.50% and 43.06%, HDL-cholesterol by 22.16 and 30.15% and artherogenic index by 69.78 and 74.43%, respectively at the doses of 200 and 400 mg/kg, as compared to untreated hypertensive rats. A. floribunda (200 and 400 mg/kg) decrease bilirubine (12.98 and 16.88%), urea (23.32% and 32.26 %), ALT (10.73 and 27.97%) and AST (29.80 and 42.22%) of treated AHR, respectively. The plant extract also reduced superoxide dismutase (SOD), malondialdehyde (MDA) and catalase and increased the reduced glutathione (GSH) concentration in aorta, heart, kidney and liver of AHR. Conclusion: These results suggest that the aqueous extract of A. floribunda possesses antioxidant and hypotensive activity in alcohol-induced hypertension

scholarly journals Antioxidant Properties and Digestive Enzyme Inhibitory Activity of the Aqueous Extract from Leafy Stems of Cissus polyantha

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Mahamad Abba Talba ◽  
David Miaffo ◽  
Sylviane Laure Poualeu Kamani ◽  
Albert Kamanyi ◽  
Sylvie Léa Wansi
Keyword(s):  
Traditional Medicine ◽  
Aqueous Extract ◽  
Inhibitory Activity ◽  
Antiradical Activity ◽  
Digestive Enzyme ◽  
Antioxidant Properties ◽  
Antioxidant Potential ◽  
Antioxidant Power ◽  
Treatment Of Diabetes

Cissus polyantha (Vitaceae) is a plant used in Cameroonian traditional medicine for the treatment of diabetes. The aims of this study were to evaluate the in vitro antioxidant and antidiabetic activities of the aqueous extract of Cissus polyantha leafy stems. The enzyme inhibitory activity was determined in vitro on α-amylase and α-glucosidase enzymes, followed by confirmative study in vivo on normal rats (oral starch and sucrose tolerance tests at doses of 111, 222, and 444 mg/kg). The ferric reducing antioxidant power and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) antiradical activity of the extract were examined to evaluate the antioxidant potential of the extract. The total content of phenols, flavonoids, and tannins of the extract were also determined. The results showed an inhibitory effect of the extract on the α-amylase and α-glucosidase activities with IC50 values of 216.14 and 182.40 μg/mL, respectively. The extract at doses of 222 and 444 mg/kg induced a significant decrease in postprandial glycaemia during the starch and sucrose tolerance tests. A remarkable antiradical activity of the extract was obtained although lower than that of the standard product. The aqueous extract of leafy stems of Cissus polyantha has an interesting inhibitory activity on the α-amylase and α-glucosidase enzymes, as well as an antioxidant potential, thus validating its use in traditional medicine for the treatment of diabetes mellitus and its complications.

Green Synthesis of Silver Nanostructures Using Aqueous Extract of Dracocephalum kotschyi and Evaluation of Antioxidant Properties of Herbal Extracts and Antibacterial Feature of Green- Synthesized Nanostructures

2019 ◽  
Vol 13 (3) ◽  
pp. 223-231
Author(s):  
Zahra Goli ◽  
Cobra Izanloo
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Size Distribution ◽  
Aqueous Extract ◽  
Nitrate Solution ◽  
Antioxidant Properties ◽  
Herbal Extract ◽  
Silver Nanostructures ◽  
Dracocephalum Kotschyi ◽  
Vis Spectroscopy

Background: Silver nanoparticles have a profound role in the field of high sensitivity biomolecular detection, catalysis, biosensors and medicine. In the present study, aqueous extract of Dracocephalum kotschyi has been used for the synthesis of silver nanoparticles. Objective: In this study, we evaluated the antioxidant features and the possibility of biosynthesis of AgNPs using an aqueous extract of Dracocephalum kotschyi and also evaluated the antibacterial activities of the synthesized nanoparticles. Methods: An eco-friendly and cost-effective protocol for the synthesis of Ag nanoparticles by utilizing a renewable natural resource, aqueous solution of Dracocephalum kotschyi, was proposed. Synthesized nanoparticles were characterized by UV–Vis spectroscopy, SEM, EDS, and XRD pattern. Results: At first, the extract of Dracocephalum kotschyi was assessed to determine and confirm the presence of an antioxidant feature. Resuscitation of one mM silver nitrate solution was carried out by the herbal extract. The solution containing AgNPs obtained from green synthesis had a maximum optical density at 225 nm. In addition, the presence of AgNPs was approved by energy-dispersive X-ray spectroscopy (EDS). Images of the scanning electron microscope demonstrated that the synthesized AgNPs had the shape of rods and the size distribution of 48-51 nm. One of the benefits of this method is a uniform size distribution. Moreover, the effects of reaction time and concentration of the herbal extract were assessed by ultraviolet-visible (UV-Vis) spectroscopy. In the end, we assessed the antibacterial impact of the synthesized AgNPs against some pathogenic bacterial strains. According to the results, the produced nanostructures had a proper impact on two bacteria of Escherichia coli and Staphylococcus aureus. Conclusion: According to the results of the present study, Dracocephalum kotschyi can be a suitable compound for the synthesis of nanostructures due to its indigenous cultivation and great medicinal properties.

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