Green Synthesis of Silver Nanostructures Using Aqueous Extract of Dracocephalum kotschyi and Evaluation of Antioxidant Properties of Herbal Extracts and Antibacterial Feature of Green- Synthesized Nanostructures

2019 ◽  
Vol 13 (3) ◽  
pp. 223-231
Author(s):  
Zahra Goli ◽  
Cobra Izanloo
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Size Distribution ◽  
Aqueous Extract ◽  
Nitrate Solution ◽  
Antioxidant Properties ◽  
Herbal Extract ◽  
Silver Nanostructures ◽  
Dracocephalum Kotschyi ◽  
Vis Spectroscopy

Background: Silver nanoparticles have a profound role in the field of high sensitivity biomolecular detection, catalysis, biosensors and medicine. In the present study, aqueous extract of Dracocephalum kotschyi has been used for the synthesis of silver nanoparticles. Objective: In this study, we evaluated the antioxidant features and the possibility of biosynthesis of AgNPs using an aqueous extract of Dracocephalum kotschyi and also evaluated the antibacterial activities of the synthesized nanoparticles. Methods: An eco-friendly and cost-effective protocol for the synthesis of Ag nanoparticles by utilizing a renewable natural resource, aqueous solution of Dracocephalum kotschyi, was proposed. Synthesized nanoparticles were characterized by UV–Vis spectroscopy, SEM, EDS, and XRD pattern. Results: At first, the extract of Dracocephalum kotschyi was assessed to determine and confirm the presence of an antioxidant feature. Resuscitation of one mM silver nitrate solution was carried out by the herbal extract. The solution containing AgNPs obtained from green synthesis had a maximum optical density at 225 nm. In addition, the presence of AgNPs was approved by energy-dispersive X-ray spectroscopy (EDS). Images of the scanning electron microscope demonstrated that the synthesized AgNPs had the shape of rods and the size distribution of 48-51 nm. One of the benefits of this method is a uniform size distribution. Moreover, the effects of reaction time and concentration of the herbal extract were assessed by ultraviolet-visible (UV-Vis) spectroscopy. In the end, we assessed the antibacterial impact of the synthesized AgNPs against some pathogenic bacterial strains. According to the results, the produced nanostructures had a proper impact on two bacteria of Escherichia coli and Staphylococcus aureus. Conclusion: According to the results of the present study, Dracocephalum kotschyi can be a suitable compound for the synthesis of nanostructures due to its indigenous cultivation and great medicinal properties.

scholarly journals Green Synthesis of Silver Nanoparticles Using Some Medicinal Plants

2020 ◽  
pp. 13-26
Author(s):  
Joud Jalab ◽  
Adawia Kitaz ◽  
Wassim Abdelwahed ◽  
Rawaa Al- Kayali
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Nitrate Solution ◽  
Chemical Properties ◽  
Silver Nitrate Solution ◽  
Average Diameter ◽  
Total Phenolic ◽  
Acacia Cyanophylla ◽  
Vis Spectroscopy

Aims: The green synthesis of silver nanoparticles in an eco-friendly, economical and more effective approach using (Acacia cyanophylla, Phlomis syriaca and Scolymus hispanicus) plants extracts and describing their main chemical properties and study the effect of its chemical composition on producing silver nanoparticles. Methodology: In this study, aqueous and ethanolic extracts of the three plants were evaluated for antioxidant activity using 2,2-diphenyl-l-picrylhydrazyl (DPPH) assay, Total polyphenol and flavonoid contents were determined using spectrophotometric method, but  total saponins were determined by weight method, The synthesis of silver nanoparticles was performed by a reduction method using aqueous silver nitrate solution and aqueous extracts of the three plants. Then study its characterization in a number of ways, such as visual inspection, UV-Vis spectroscopy and dynamic light scattering. Results: The results showed that the total phenolic content ranged in extracts between (13.08 ±2.279 to 98.39 ±4.755 mg GAE/g DW). While the total flavonoid contents varied from (19.83 ±2.384 to 121.64 ±6.469 mg RE/g DW. Antioxidant activity was expressed as IC50 and the obtained results ranged from (IC50= 0.027 ±0.00038 to 0.878 ±0.045 mg/ml), the results indicated that the ethanolic Acacia cyanophylla extract from the six examined extracts showed the highest phenolic and flavonoid concentration and strong antioxidant activity. Also, the saponins content in the three plants ranged from (0.46 to 2.53)% and the highest amount of saponins reported in Acacia cyanophylla plant. The silver nanoparticles prepared using Acacia cyanophylla extract have reported visible yellowish brown color formation and the absorption peak at 460 nm indicates the biosynthesis of silver nanoparticles and they have average diameter (134.1) nm and the polydispersity index (PdI) was suitable (0.260). Conclusion: Acacia cyanophylla extract has been considered as the best reducing agent among the selected plant extracts for the preparation of stable colloidal silver nanoparticles, this is due to their high content of flavonoids, phenols and saponins.

scholarly journals Investigating the Possibility of Green Synthesis of Silver Nanoparticles Using Vaccinium arctostaphlyos Extract and Evaluating Its Antibacterial Properties

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Sedighe Khodadadi ◽  
Nafiseh Mahdinezhad ◽  
Bahman Fazeli-Nasab ◽  
Mohammad Javad Heidari ◽  
Baratali Fakheri ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Ftir Spectroscopy ◽  
Aqueous Extract ◽  
Nitrate Solution ◽  
Antibacterial Properties ◽  
Silver Nitrate Solution ◽  
X Ray Diffraction ◽  
X Ray ◽  
Vis Spectroscopy

Objective. Vaccinium genus plants have medicinal value, of which Vaccinium arctostaphylos (Caucasian whortleberry or Qare-Qat in the local language) is the only available species in Iran. Public tendency to use herbal remedies and natural products such as synthesized nanoparticles is increasing due to the proof of the destructive side effects of chemical drugs. Nanosilver products have been effective against more than 650 microbe types. This study was aimed at assessing the possibility of green synthesis of silver nanoparticles using Vaccinium arctostaphylos aqueous extract and at evaluating its antibacterial properties, as well. Materials and Methods. In order to synthesize silver nanoparticles, different volumes of Vaccinium arctostaphylos aqueous extract (3, 5, 10, 15, and 30 ml) were assessed with different silver nitrate solution concentrations (0.5, 1, 3, 5, and 10 mM) and different reaction time durations (1, 3, 5, 10, and 20 minutes) at room temperature using a rotary shaker with a speed of 150 rpm. Ultraviolet-visible (UV-Vis) spectroscopy, X-ray diffraction analysis (XRD), Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM) were carried out. The antibacterial activity of the aqueous extract and the synthesized nanoparticles was evaluated, as well. Results. Silver nanoparticle formation process was confirmed with XRD analysis, transmission electron microscopy (TEM), and FTIR spectroscopy. The UV-Vis spectroscopy of silver colloidal nanoparticles showed a surface plasmon resonance peak at 443 nm under optimal conditions (3 ml aqueous extract volume, 1 mM silver nitrate solution concentration, and 3 min reaction time under sunlight exposure). The reduction of silver ions to silver nanoparticles in solution was confirmed, as well. Based on X-ray diffraction analysis, the size of silver nanoparticles was in the range of 7-16 nm. TEM images showed an even distribution of silver nanoparticles, with a spherical shape. FTIR spectroscopy demonstrated the presence of different functional groups of oxygenated compounds such as carboxyl, hydroxyl, and nitrogenous groups. The antibacterial properties of the synthesized nanoparticles were confirmed. Conclusion. The synthesized nanoparticles showed more antibacterial properties against gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) than gram-negative ones (Escherichia coli and Salmonella enteritidis).

scholarly journals One-pot green synthesis of gold and silver nanoparticles using Rosa canina L. extract

RSC Advances ◽  
2021 ◽  
Vol 11 (24) ◽  
pp. 14624-14631
Author(s):  
Pablo Eduardo Cardoso-Avila ◽  
Rita Patakfalvi ◽  
Carlos Rodríguez-Pedroza ◽  
Xochitl Aparicio-Fernández ◽  
Sofía Loza-Cornejo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Aqueous Extract ◽  
Room Temperature ◽  
One Pot ◽  
Gold And Silver Nanoparticles ◽  
Rosa Canina ◽  
Capping Agents

Gold and silver nanoparticles were synthesized at room temperature using an aqueous extract from dried rosehips acting as reducing and capping agents with no other chemicals involved.

scholarly journals Microwave-assisted green synthesis of silver nanoparticles using dried extracts of Chlorella vulgaris and antibacterial activity studies

2020 ◽  
Vol 9 (1) ◽  
pp. 283-293
Author(s):  
Milad Torabfam ◽  
Meral Yüce
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Chlorella Vulgaris ◽  
Metallic Nanoparticles ◽  
Nitrate Solution ◽  
Spherical Shape ◽  
Silver Nitrate Solution ◽  
Spectroscopy Analysis ◽  
Bacterial Diseases ◽  
Serovar Typhimurium

AbstractGreen synthesis of metallic nanoparticles (NPs) is acquiring considerable attention due to its environmental and economic superiorities over other methods. This study describes the practical synthesis of silver nanoparticles (AgNPs) through the reduction of silver nitrate solution using an algal source, Chlorella vulgaris, as the reducing as well as the stabilizing agent. The energy required for this synthesis was supplied by microwave radiation. The ultraviolet-visible spectroscopy exhibited a single peak related to the surface plasmon absorbance of AgNPs at 431 nm. The AgNPs with high stability (a zeta potential of −17 mV), hydrodynamic size distribution of 1–50 nm, and mostly spherical shape were obtained through a 10 min process. Fourier transform infrared spectroscopy analysis revealed that several functional groups, including carbonyl groups of C. vulgaris, play a significant role in the formation of functional NPs. Antibacterial features of the produced AgNPs were verified against those of Salmonella enterica subsp. enterica serovar typhimurium and Staphylococcus aureus, demonstrating a considerable growth inhibition at increasing concentrations of the NPs. As a result, the formed AgNPs can be used as a promising agent against bacterial diseases.

scholarly journals Green Synthesis, Characterization, and Antibacterial Properties of Silver Nanoparticles Obtained by Using Diverse Varieties of Cannabis sativa Leaf Extracts

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4041
Author(s):  
Adriana Cecilia Csakvari ◽  
Cristian Moisa ◽  
Dana G. Radu ◽  
Leonard M. Olariu ◽  
Andreea I. Lupitu ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Antioxidant Capacity ◽  
Cannabis Sativa ◽  
Antibacterial Properties ◽  
Green Building ◽  
Human Pathogens ◽  
Leaf Extracts ◽  
Vis Spectroscopy ◽  
Ft Ir

Cannabis sativa L. (hemp) is a plant used in the textile industry and green building material industry, as well as for the phytoremediation of soil, medical treatments, and supplementary food products. The synergistic effect of terpenes, flavonoids, and cannabinoids in hemp extracts may mediate the biogenic synthesis of metal nanoparticles. In this study, the chemical composition of aqueous leaf extracts of three varieties of Romanian hemp (two monoecious, and one dioecious) have been determined by Fourier-Transformed Infrared spectroscopy (FT-IR), high-performance liquid chromatography, and mass spectrometry (UHPLC-DAD-MS). Then, their capability to mediate the green synthesis of silver nanoparticles (AgNPs) and their pottential antibacterial applications were evaluated. The average antioxidant capacity of the extracts had 18.4 ± 3.9% inhibition determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 78.2 ± 4.1% determined by 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS™) assays. The total polyphenolic content of the extracts was 1642 ± 32 mg gallic acid equivalent (GAE) L−1. After this, these extracts were reacted with an aqueous solution of AgNO3 resulting in AgNPs, which were characterized by UV−VIS spectroscopy, FT-IR, scanning electron microscopy (SEM-EDX), and dynamic light scattering (DLS). The results demonstrated obtaining spherical, stable AgNPs with a diameter of less than 69 nm and an absorbance peak at 435 nm. The mixture of extracts and AgNPs showed a superior antioxidant capacity of 2.3 ± 0.4% inhibition determined by the DPPH• assay, 88.5 ± 0.9% inhibition as determined by the ABTS•+ assay, and a good antibacterial activity against several human pathogens: Escherichia coli, Klebsiella pneumoniae, Pseudomonas fluorescens, and Staphylococcus aureus.

Green synthesis of silver nanoparticles using aqueous extract of dried Juglans regia green husk and examination of its biological properties

2017 ◽  
Vol 6 (5) ◽  
Author(s):  
Zahra Abbasi ◽  
Sholeh Feizi ◽  
Elham Taghipour ◽  
Parinaz Ghadam
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Aqueous Extract ◽  
Plant Extract ◽  
Room Temperature ◽  
Juglans Regia ◽  
Biological Properties ◽  
Rapid Process

AbstractSilver nanoparticles (AgNPs) have widespread applications. Recently, the synthesis of NPs using plant extract has attracted much attention. In this study, with an easy and rapid process at room temperature, AgNPs were produced by the aqueous extract of dried

scholarly journals Optimization of Green Synthesis of Silver Nanoparticles from Leaf Extracts ofPimenta dioica(Allspice)

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Akshay Rajeev Geetha ◽  
Elizabeth George ◽  
Akshay Srinivasan ◽  
Jameel Shaik
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Leaf Extract ◽  
Synthesis Method ◽  
Leaf Extracts ◽  
Synthesis Of Nanoparticles ◽  
Hot Air ◽  
Vis Spectroscopy ◽  
Pimenta Dioica ◽  
First Time

Production of silver nanoparticles from the leaf extracts ofPimenta dioicais reported for the first time in this paper. Three different sets of leaves were utilized for the synthesis of nanoparticles—fresh, hot-air oven dried, and sun-dried. These nanoparticles were characterized using UV-Vis spectroscopy and AFM. The results were diverse in that different sizes were seen for different leaf conditions. Nanoparticles synthesized using sun-dried leaves (produced using a particular ratio (1 : 0.5) of the leaf extract sample and silver nitrate (1 mM), resp.) possessed the smallest sizes. We believe that further optimization of the current green-synthesis method would help in the production of monodispersed silver nanoparticles having great potential in treating several diseases.

PHYTO-MEDIATED SYNTHESIS OF SILVER NANOPARTICLES USING AQUEOUS EXTRACT OF „BUGLOSSOIDES PURPUROCAERULEA“ (BORAGINACEAE) AND THEIR BIOACTIVITY

2021 ◽  
Author(s):  
Jelena S. Katanić Stanković ◽  
◽  
Nikola Srećković ◽  
Vladimir Mihailović
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Bacterial Strains ◽  
Penicillium Species ◽  
Microdilution Method ◽  
Biological Potential ◽  
Vis Spectroscopy

In this study, silver nanoparticles (AgNPs) have been synthesized using the aqueous extract of the aerial parts of B. purpurocaerulea, collected in Serbia. B. purpurocaerulea silver nanoparticles (Bp– AgNPs) synthesis was confirmed using UV-Vis spectroscopy and Fourier Transform Infrared Spectroscopy (FTIR). The biological potential of synthesized Bp-AgNPs was evaluated in vitro using ABTS assay for determining free radical scavenging potential and microdilution method for analysis of antimicrobial properties. Bp-AgNPs showed high antioxidant activity similar to Bp-extract, comparable to BHT. The synthesized nanoparticles exerted remarkable antibacterial effects, with minimal inhibitory concentration (MIC) values below 20 µg/mL. In the case of some bacterial strains, the results of Bp– AgNPs were comparable or similar to standard antibiotic erythromycin. The antifungal activity of Bp– AgNPs was moderate for most of the used strains. Nevertheless, several fungi were resistant to the NPs action, while two tested Penicillium species were extremely sensitive on Bp-AgNPs with MIC lower than 40 µg/mL. The antimicrobial properties of Bp-AgNPs can be useful for the development of new NPs-containing products.

Controlling the Surface Plasmon Absorption of Silver Nanoparticles via Green Synthesis Using Pennisetum purpureum Leaf Extract

2018 ◽  
Vol 772 ◽  
pp. 73-77
Author(s):  
Ruelson S. Solidum ◽  
Arnold C. Alguno ◽  
Rey Capangpangan
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Silver Nitrate ◽  
Surface Plasmon ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Silver Nitrate Solution ◽  
Absorption Peak ◽  
Plasmon Absorption ◽  
Surface Plasmon Absorption

We report on the green synthesis of silver nanoparticles utilizing theP.purpureumleaf extract. Controlling the surface plasmon absorption of silver nanoparticles was achieved by regulating the amount of extract concentration and the molarity of silver nitrate solution. The surface plasmon absorption peak is found at around 430nm. The surface plasmon absorption peak have shifted to lower wavelength as the amount of extract is increased, while plasmon absorption peak shifts on a higher wavelength as the concentration of silver nitrate is increased before it stabilized at 430nm. This can be explained in terms of the available nucleation sites promoted by the plant extract as well as the available silver ions present in silver nitrate solution.

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