Combining experimental techniques with molecular dynamics to investigate the impact of different enzymatic hydrolysis of β-lactoglobulin on the antigenicity reduction

2021 ◽  
Vol 350 ◽  
pp. 129139
Author(s):  
Xin-Xin Yu ◽  
Wei-Yue Liang ◽  
Jia-Yi Yin ◽  
Qian Zhou ◽  
Dong-Mei Chen ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Enzymatic Hydrolysis ◽  
Experimental Techniques ◽  
The Impact ◽  
Β Lactoglobulin ◽  
Hydrolysis Of

scholarly journals Alkali Pretreatment and Enzymatic Hydrolysis of Australian Timber Mill Sawdust for Biofuel Production

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Raymond Martin Trevorah ◽  
Maazuza Z. Othman
Keyword(s):  
Enzymatic Hydrolysis ◽  
Simultaneous Saccharification And Fermentation ◽  
Glucose Production ◽  
Biofuel Production ◽  
Alkali Pretreatment ◽  
Ethanol Yields ◽  
The Impact ◽  
Hydrolysis Of ◽  
Potential Use ◽  
Simultaneous Saccharification

This study investigated the potential use of alkali pretreatment of sawdust from Australian timber mills to produce bioethanol. Sawdust was treated using 3–10% w/w NaOH at temperatures of 60, 121, and −20°C. Two pathways of production were trialled to see the impact on the bioethanol potential, enzymatic hydrolysis for glucose production, and simultaneous saccharification and fermentation (SSF) for ethanol production. The maximum yields obtained were at 121°C and −20°C using 7% NaOH, with 29.3% and 30.6% ethanol yields after 0.5 and 24 hr, respectively, these treatments yielded 233% and 137% increase from the 60°C counter parts. A notable trend of increased ethanol yields with increased NaOH concentration was observed for samples treated at 60°C; for example, samples treated using 10% NaOH produced 1.92–2.07 times more than those treated using 3% NaOH. FTIR analysis showed reduction in crystallinity correlating with increased ethanol yields with the largest reduction in crystallinity in the sample treated at −20°C for 24 hr with 7% NaOH.

scholarly journals Membrane Filtration-Assisted Enzymatic Hydrolysis Affects the Biological Activity of Potato Juice

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 852
Author(s):  
Przemysław Łukasz Kowalczewski ◽  
Anna Olejnik ◽  
Iga Rybicka ◽  
Magdalena Zielińska-Dawidziak ◽  
Wojciech Białas ◽  
...  
Keyword(s):  
Biological Activity ◽  
Enzymatic Hydrolysis ◽  
Cell Line ◽  
Membrane Filtration ◽  
Nutritional Value ◽  
Protein Hydrolysate ◽  
Gastrointestinal Symptoms ◽  
Human Colon Cancer ◽  
The Impact ◽  
Hydrolysis Of

The results of recently published studies indicate that potato juice is characterized by interesting biological activity that can be particularly useful in the case of gastrointestinal symptoms. Moreover, the studies also described the high nutritional value of its proteins. This article is a report on the impact of the enzymatic hydrolysis of proteins combined with membrane filtration. The obtained potato juice protein hydrolysate (PJPH) and its concentrate (cPJPH) were characterized in terms of their nutritional value and biological activity. The amino acid profile and scoring, the content of mineral compounds, and the antioxidant and in vitro cytotoxic activity were assessed. The study proved that the antioxidant activity of PJPH is higher than that of fresh potato juice, and the cytotoxicity against human gastric carcinoma cell line (Hs 746T), human colon cancer cell line (Caco-2), human colorectal adenocarcinoma cell line (HT-29), and human normal colon mucosa cell line (CCD 841 CoN) showed biological activity specifically targeted against cancer cells. Therefore, it can be concluded that the membrane filtration-assisted enzymatic hydrolysis of potato juice proteins may increase their biological activity and allow for potato juice to be used in the production of medicinal preparations.

scholarly journals Design and performance of amphiphilic lignin derivatives in enzymatic hydrolysis of sweet sorghum bagasse for bioethanol production

BioResources ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. 5875-5889
Author(s):  
Nissa Nurfajrin Solihat ◽  
Rika Raniya ◽  
Triyani Fajriutami ◽  
Apri Heri Iswanto ◽  
Widya Fatriasari ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sweet Sorghum ◽  
Cellulose Content ◽  
Sweet Sorghum Bagasse ◽  
Bioethanol Production ◽  
Hydrolysis Time ◽  
Peg 4000 ◽  
Sorghum Bagasse ◽  
The Impact ◽  
Hydrolysis Of

Sweet sorghum bagasse (SSB) is potential feedstock for bioethanol production due to its natural abundance and high cellulose content (> 40%). This work compared the impact of three variables relative to the enzymatic hydrolysis of SSB kraft pulp. The three variables were the biosurfactant from lignin derivative known as amphiphilic lignin derivatives (A-LD), the enzyme loading level, and the hydrolysis time. These variables were optimized by response surface methodology (RSM) with a Box-Behnken design (BBD). The concentration of polyethylene glycol (PEG) 4000 was also optimized to compare it with the A-LD performance in the enzymatic hydrolysis process. After optimization, the A-LD produced a higher reducing sugar yield (RSY) (99.45%) than the PEG 4000. The difference in the predicted versus experimental values of the RSY was less than 4%, which means that the model was highly predictive. The adequacy of the model was confirmed by a regression value close to 1 for the A-LD assisted test. The result implies that the A-LD significantly improved the enzymatic hydrolysis performance to enhance the RSY. Moreover, the BBD is adequate and useful to identify the optimum concentration of surfactant.

Comparison of the principal proteins in bovine, caprine, buffalo, equine and camel milk

2012 ◽  
Vol 79 (2) ◽  
pp. 185-191 ◽  
Author(s):  
Katharina Hinz ◽  
Paula M O'Connor ◽  
Thom Huppertz ◽  
R Paul Ross ◽  
Alan L Kelly
Keyword(s):  
Enzymatic Hydrolysis ◽  
Whey Protein ◽  
Proteomic Analysis ◽  
Bovine Milk ◽  
Camel Milk ◽  
Interspecies Differences ◽  
Milk Samples ◽  
Equine Milk ◽  
Β Lactoglobulin ◽  
Hydrolysis Of

Proteomic analysis of bovine, caprine, buffalo, equine and camel milk highlighted significant interspecies differences. Camel milk was found to be devoid of β-lactoglobulin, whereas β-lactoglobulin was the major whey protein in bovine, buffalo, caprine, and equine milk. Five different isoforms of κ-casein were found in camel milk, analogous to the micro-heterogeneity observed for bovine κ-casein. Several spots observed in 2D-electrophoretograms of milk of all species could tentatively be identified as polypeptides arising from the enzymatic hydrolysis of caseins. The understanding gained from the proteomic comparison of these milks may be of relevance both in terms of identifying sources of hypoallergenic alternatives to bovine milk and detection of adulteration of milk samples and products.

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