Effects of extraction methods on the digestibility, cytotoxicity, prebiotic potential and immunomodulatory activity of taro (Colocasia esculenta) water-soluble non-starch polysaccharide

2021 ◽  
pp. 107068
Author(s):  
Mylene Anwar ◽  
Sonya Mros ◽  
Michelle McConnell ◽  
Alaa El-Din Bekhit
Keyword(s):  
Extraction Methods ◽  
Colocasia Esculenta ◽  
Water Soluble ◽  
Immunomodulatory Activity

New advances in the method validation, extraction methods and measurement uncertainty for the determination of water-soluble hexavalent chromium in hydraulic cement

Talanta ◽  
2021 ◽  
Vol 232 ◽  
pp. 122286
Author(s):  
María Melania Ramírez-Quesada ◽  
Jimmy Venegas-Padilla ◽  
José Pablo Sibaja-Brenes ◽  
Bryan Calderón-Jiménez
Keyword(s):  
Measurement Uncertainty ◽  
Hexavalent Chromium ◽  
Method Validation ◽  
Extraction Methods ◽  
Water Soluble ◽  
Hydraulic Cement

THE SOLUBLE PROTEINS OF THE MUSCLE TISSUE OF THE HADDOCK

1931 ◽  
Vol 6 (1) ◽  
pp. 1-11 ◽  
Author(s):  
J. F. LOGAN
Keyword(s):  
Sodium Chloride ◽  
Aqueous Solutions ◽  
Muscle Tissue ◽  
Soluble Protein ◽  
Potassium Phosphate ◽  
Extraction Methods ◽  
Water Soluble ◽  
Tabular Form ◽  
Water Soluble Protein ◽  
Isoelectric Points

As a contribution to the chemistry of muscle tissue, the solubility of the protein of haddock muscle in aqueous solutions of sodium chloride and neutral potassium phosphate, respectively, was determined. The results are expressed in tabular form and graphically in the form of solubility curves. A water-soluble protein and also a salt-soluble protein were isolated from dialyzed haddock muscle by extraction methods. These proteins were obtained in a comparatively pure condition by precipitation from solution in the region of their isoelectric points.

Acidic Aqueous Extraction of Hypothalamic Luteinizing Hormone Releasing Hormone to Study Biological Effects

1974 ◽  
Vol 52 (3) ◽  
pp. 754-758 ◽  
Author(s):  
S. H. Shin ◽  
C. J. Howitt
Keyword(s):  
Luteinizing Hormone ◽  
Biological Effects ◽  
Extraction Procedure ◽  
Extraction Methods ◽  
Water Soluble ◽  
Acid Extraction ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Aqueous Solvent ◽  
Lh Rh

Several aqueous solvent systems were tested for their efficiency in extracting luteinizing hormone releasing hormone (LH-RH) from rat hypothalamus. Although LH-RH is a water-soluble decapeptide, neutral distilled water extracted only 10% of the LH-RH obtained using acid extraction methods. The efficiency of the acid extraction procedure suggests that in the hypothalamus the releasing hormone is bound to a relatively large molecular weight compound. Using the acidic extraction procedure, we found that hypothalamic LH-RH content is significantly lower in the castrated animal than in the normal rat.

scholarly journals High molecular weight humic-like substances in carboneous aerosol of Ulaanbaatar city

2018 ◽  
Vol 19 (45) ◽  
pp. 5-11 ◽  
Author(s):  
Shurkhuu Tserenpil ◽  
Xing Jung Fan ◽  
Atindra Sapkota ◽  
Enkhmaa Chinzorig ◽  
Jian Zhong Song ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Carbon Content ◽  
High Molecular Weight ◽  
Solid Phase ◽  
Extraction Methods ◽  
Water Soluble ◽  
Total Carbon ◽  
Total Carbon Content ◽  
Different Seasons ◽  
Soluble Organic Fraction

Total carbon content of the atmospheric suspended particulate matters consisted of as high as 89-93% organic carbon (OC) in Ulaanbaatar aerosol without showing seasonal variation. However, limited aerosol measurements have been conducted on these OC rich aerosols particularly for high molecular weight constituents. In order to address the gap above, abundance of high molecular weight humic-like substances (HULIS) in total suspended particulates (TSP) from Ulaanbaatar atmospheric aerosol were determined for the first time. HULIS molecular structure was characterised for different seasons using carbon content and UV absorbance measurements coupled with solid phase extraction methods. Although, HULIS contributions to water soluble organic fraction of the winter and summer aerosols were similar HULIS carbon concentration was higher in winter samples (9-37 mg·L-1) than in summer (2-6 mg·L-1). Consequently quantity of aromatic moieties and degree of aromaticity varied between seasons.

scholarly journals Anticancer and Immunomodulatory Activities of a Novel Water-Soluble Derivative of Ellipticine

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2130
Author(s):  
Regiane Costa de Oliveira ◽  
Gemilson Soares Pontes ◽  
Aleksandr Kostyuk ◽  
Gabriel B. Coutinho Camargo ◽  
Anamika Dhyani ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Line ◽  
K562 Cell ◽  
Water Soluble ◽  
Health Concern ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunomodulatory Activity ◽  
K562 Cell Line ◽  
Human Pbmc ◽  
Cancerous Cells

Cancer still remains a major public health concern around the world and the search for new potential antitumor molecules is essential for fighting the disease. This study evaluated the anticancer and immunomodulatory potential of the newly synthetized ellipticine derivate: sodium bromo-5,11-dimethyl-6H-pyrido[4,3-b]carbazole-7-sulfonate (Br-Ell-SO3Na). It was prepared by the chlorosulfonation of 9-bromoellipticine. The ellipticine-7-sulfonic acid itself is not soluble, but its saponification with sodium hydroxide afforded a water-soluble sodium salt. The cytotoxicity of Br-Ell-SO3Na was tested against cancerous (K562 cell line) and non-cancerous cells (Vero cell line and human peripheral blood mononuclear cells (PBMC)) using a Methylthiazoletetrazolium (MTT) assay. Cell cycle arrest was assessed by flow cytometry and the immunomodulatory activity was analyzed through an enzyme-linked immunosorbent assay (ELISA). The results showed that the Br-Ell-SO3Na molecule has specific anticancer activity (IC50 = 35 µM) against the K562 cell line, once no cytotoxicity effect was verified against non-cancerous cells. Cell cycle analysis demonstrated that K562 cells treated with Br-Ell-SO3Na were arrested in the phase S. Moreover, the production of IL-6 increased and the expression of IL-8 was inhibited in the human PBMC treated with Br-Ell-SO3Na. The results demonstrated that Br-Ell-SO3Na is a promising anticancer molecule attested by its noteworthy activity against the K562 tumor cell line and immunomodulatory activity in human PBMC cells.

Tris-imidazole tripodands: synthesis and complexation

1989 ◽  
Vol 67 (8) ◽  
pp. 1363-1369 ◽  
Author(s):  
Pierre G. Potvin ◽  
Roberto Gau ◽  
Cathleen M. Unrau ◽  
Nikolaus K. Gehl
Keyword(s):  
Extraction Methods ◽  
Water Soluble ◽  
Extraction Selectivity

Simple syntheses of seven imidazole-terminated tripodands are described. Some are N-methylated and water-soluble and others are N-benzylated and only lipo-soluble. Pronounced and unusual selectivity for hydrazinium ion was found in complexation experiments using Cram's extraction methods. Keywords: imidazole, tripodands, extraction, selectivity, hydrazinium.

scholarly journals LCMS-QTOF Determination of Lentinan-Like β-D-Glucan Content Isolated by Hot Water and Alkaline Solution from Tiger’s Milk Mushroom, Termite Mushroom, and Selected Local Market Mushrooms

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Nor Azreen Mohd Jamil ◽  
Norasfaliza Rahmad ◽  
Noraswati Mohd Nor Rashid ◽  
Mohd Hafis Yuswan Mohd Yusoff ◽  
Nur Syahidah Shaharuddin ◽  
...  
Keyword(s):  
Alkaline Solution ◽  
Extraction Methods ◽  
Hot Water ◽  
Immunomodulatory Activity ◽  
Local Market ◽  
Lentinus Edodes ◽  
Shiitake Mushroom ◽  
Button Mushroom ◽  
Water Extracts

Lentinan, 1152 Dalton β-D-glucan found in Shiitake Mushroom (Lentinus edodes), has been claimed to have anticancer and immunomodulatory activity. Several extraction methods have been used by researchers to isolate Lentinan including hot water and alkaline solution (1.25 M NaOH). In this study, hot water and alkaline solution (1.25 M NaOH) were used to extract the Lentinan-like β-D-glucan (1151 Dalton) from Tiger’s Milk Mushroom, Termite Mushroom, and selected local market mushrooms. The isolated Lentinan-like β-D-glucan from both hot water and alkaline solution was analyzed by LCMS-QTOF. Commercial Lentinan standard from Lentinus edodes was used as a reference. The results showed significant differences on chromatogram patterns of Lentinan-like β-D-glucan between both extracts. The peak of Lentinan-like β-D-glucan was only found in isolated polysaccharide glucan of hot water extracts. The isolated polysaccharide glucans from Tiger’s Milk Mushroom and Termite Mushroom were found to have 0.74±0.12 μg/mg and 0.53±0.07 μg/mg Lentinan-like β-D-glucan. Button Mushroom, Shiitake Mushroom, and Oyster Mushroom showed the presence of Lentinan-like β-D-glucan at 16.16±4.15 μg/mg, 0.22±0.04, and 0.10±0.01 μg/mg, respectively.

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