A novel three-dimensional tubular scaffold prepared from silk fibroin by electrospinning

2009 ◽  
Vol 45 (5) ◽  
pp. 504-510 ◽  
Author(s):  
Juan Zhou ◽  
Chuanbao Cao ◽  
Xilan Ma
Keyword(s):  
Silk Fibroin ◽  
Three Dimensional ◽  
Tubular Scaffold

scholarly journals Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?

2021 ◽  
Vol 22 (7) ◽  
pp. 3391
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska ◽  
Ewa Olewnik-Kruszkowska ◽  
Katarzyna Reczyńska ◽  
Elżbieta Pamuła
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Physicochemical Properties ◽  
Silk Fibroin ◽  
Three Dimensional ◽  
Microscopy Imaging ◽  
Maximum Deformation ◽  
One Step ◽  
Chitosan Blends ◽  
Fourier Transfer Infrared Spectroscopy

The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.

scholarly journals Comparative Study of Silk Fibroin-Based Hydrogels and Their Potential as Material for 3-Dimensional (3D) Printing

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3887
Author(s):  
Watcharapong Pudkon ◽  
Chavee Laomeephol ◽  
Siriporn Damrongsakkul ◽  
Sorada Kanokpanont ◽  
Juthamas Ratanavaraporn
Keyword(s):  
3D Printing ◽  
Silk Fibroin ◽  
Biomedical Applications ◽  
Mechanical Stability ◽  
Three Dimensional ◽  
Structure Stability ◽  
3 Dimensional ◽  
Critical Technology ◽  
Box Structure ◽  
High Degree

Three-dimensional (3D) printing is regarded as a critical technology in material engineering for biomedical applications. From a previous report, silk fibroin (SF) has been used as a biomaterial for tissue engineering due to its biocompatibility, biodegradability, non-toxicity and robust mechanical properties which provide a potential as material for 3D-printing. In this study, SF-based hydrogels with different formulations and SF concentrations (1–3%wt) were prepared by natural gelation (SF/self-gelled), sodium tetradecyl sulfate-induced (SF/STS) and dimyristoyl glycerophosphorylglycerol-induced (SF/DMPG). From the results, 2%wt SF-based (2SF) hydrogels showed suitable properties for extrusion, such as storage modulus, shear-thinning behavior and degree of structure recovery. The 4-layer box structure of all 2SF-based hydrogel formulations could be printed without structural collapse. In addition, the mechanical stability of printed structures after three-step post-treatment was investigated. The printed structure of 2SF/STS and 2SF/DMPG hydrogels exhibited high stability with high degree of structure recovery as 70.4% and 53.7%, respectively, compared to 2SF/self-gelled construct as 38.9%. The 2SF/STS and 2SF/DMPG hydrogels showed a great potential to use as material for 3D-printing due to its rheological properties, printability and structure stability.

scholarly journals In vivo degradation of three-dimensional silk fibroin scaffolds

Biomaterials ◽  
2008 ◽  
Vol 29 (24-25) ◽  
pp. 3415-3428 ◽  
Author(s):  
Yongzhong Wang ◽  
Darya D. Rudym ◽  
Ashley Walsh ◽  
Lauren Abrahamsen ◽  
Hyeon-Joo Kim ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Three Dimensional ◽  
In Vivo Degradation

The influence of proepicardial cells on the osteogenic potential of marrow stromal cells in a three-dimensional tubular scaffold

Biomaterials ◽  
2008 ◽  
Vol 29 (14) ◽  
pp. 2203-2216 ◽  
Author(s):  
Mani T. Valarmathi ◽  
Michael J. Yost ◽  
Richard L. Goodwin ◽  
Jay D. Potts
Keyword(s):  
Stromal Cells ◽  
Three Dimensional ◽  
Marrow Stromal Cells ◽  
Osteogenic Potential ◽  
Tubular Scaffold

scholarly journals Synergetic effects of graphene–CoPc/silk fibroin three-dimensional porous composites as catalysts for acid red G degradation

RSC Advances ◽  
2019 ◽  
Vol 9 (43) ◽  
pp. 24751-24759 ◽  
Author(s):  
Hui Ma ◽  
Huanxia Zhang ◽  
Mingqiong Tong ◽  
Jianda Cao ◽  
Wen Wu
Keyword(s):  
Silk Fibroin ◽  
Three Dimensional ◽  
Scientific Research ◽  
Dye Wastewater ◽  
Synergetic Effects ◽  
Porous Composites ◽  
Acid Red G

The disposal of dye wastewater is one of the hotspots of scientific research, and graphene–CoTAPc–SF composites exhibit more effective catalytic abilities.

Modification of Thai Silk Fibroin Scaffolds by Gelatin Conjugation for Tissue Engineering

2008 ◽  
Vol 55-57 ◽  
pp. 685-688 ◽  
Author(s):  
J. Chamchongkaset ◽  
Sorada Kanokpanont ◽  
David L. Kaplan ◽  
Siriporn Damrongsakkul
Keyword(s):  
Tissue Engineering ◽  
Bombyx Mori ◽  
Silk Fibroin ◽  
Textile Industry ◽  
Three Dimensional ◽  
Biological Properties ◽  
Salt Leaching ◽  
Biological Compatibility ◽  
Salt Crystals

Silk has been used commercially as biomedical sutures for decades. Recently silk fibroin, especially from Bombyx mori silkworm, has been explored for many tissue engineering applications such as bone and cartilage due to its impressive biological compatibility and mechanical properties. In Thailand, Thai native silkworms have been long cultivated. Distinct characteristics of cocoon Thai silk are its yellow color and coarse filament. There is more sericin in Thai silk than in other Bombyx mori silks. These characteristics provide Thai silk a unique texture for textile industry. It is therefore the aim of this study to develop three-dimensional silk fibroin-based scaffolds from Thai yellow cocoon “Nangnoi-Srisaket” of Bombyx mori silkworms using salt-leaching method. To enhance the biological properties, type A gelatin, the denature form of collagen having good biocompactibility, was used to conjugate with silk fibroin scaffolds. The pore size of salt-leached silk fibroin scaffold structure represented the size of salt crystals used (600-710µm). After gelatin conjugation, gelatin was partly formed fibers inside the pores of silk fibroin scaffolds resulting in fiber-like structure with highly interconnection. Gelatin conjugation enhanced the compressive modulus of silk fibroin scaffolds by 93%. The results on in vitro culture using mouse osteoblast-like cells (MC3T3-E1) showed that gelatin conjugation could promote the cell proliferation in silk fibroin scaffolds. Moreover, the observed morphology of cells proliferated inside the scaffold after 14 days of culture showed the larger spreading area of cells on conjugated gelatin/silk fibroin scaffolds, compared to round-shaped cells on silk fibroin scaffolds. The results implied that Thai silk fibroin looked promising to be applied in tissue engineering and gelatin conjugation on Thai silk fibroin scaffolds could enhance the biological properties of scaffolds.

scholarly journals Three-Layered Silk Fibroin Tubular Scaffold for the Repair and Regeneration of Small Caliber Blood Vessels: From Design to in vivo Pilot Tests

2019 ◽  
Vol 7 ◽  
Author(s):  
Antonio Alessandrino ◽  
Anna Chiarini ◽  
Marco Biagiotti ◽  
Ilaria Dal Prà ◽  
Giulia A. Bassani ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Silk Fibroin ◽  
Tubular Scaffold ◽  
Pilot Tests

scholarly journals Silk Fibroin/Collagen/Chitosan Scaffolds Cross-Linked by a Glyoxal Solution as Biomaterials toward Bone Tissue Regeneration

Materials ◽  
2020 ◽  
Vol 13 (15) ◽  
pp. 3433
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska ◽  
Catarina C. Coelho ◽  
Fernando J. Monteiro
Keyword(s):  
Mechanical Properties ◽  
Silk Fibroin ◽  
Tissue Regeneration ◽  
Three Dimensional ◽  
Bone Tissue Regeneration ◽  
New Materials ◽  
Swelling Degree ◽  
Chitosan Scaffolds ◽  
Measured Density ◽  
Scanning Electron

In this study, three-dimensional materials based on blends of silk fibroin (SF), collagen (Coll), and chitosan (CTS) cross-linked by glyoxal solution were prepared and the properties of the new materials were studied. The structure of the composites and the interactions between scaffold components were studied using FTIR spectroscopy. The microstructure was observed using a scanning electron microscope. The following properties of the materials were measured: density and porosity, moisture content, and swelling degree. Mechanical properties of the 3D materials under compression were studied. Additionally, the metabolic activity of MG-63 osteoblast-like cells on materials was examined. It was found that the materials were characterized by a high swelling degree (up to 3000% after 1 h of immersion) and good porosity (in the range of 80–90%), which can be suitable for tissue engineering applications. None of the materials showed cytotoxicity toward MG-63 cells.

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