Determination of aflatoxin M1 in urine samples indicates frequent dietary exposure to aflatoxin B1 in the Bangladeshi population

2017 ◽  
Vol 220 (2) ◽  
pp. 271-281 ◽  
Author(s):  
Nurshad Ali ◽  
Meinolf Blaszkewicz ◽  
Khaled Hossain ◽  
Gisela H. Degen
Keyword(s):  
Aflatoxin B1 ◽  
Dietary Exposure ◽  
Urine Samples ◽  
Aflatoxin M1 ◽  
Bangladeshi Population

Simultaneous Determination of Aflatoxin B1 and Aflatoxin M1 in Food Matrices by Enzyme-Linked Immunosorbent Assay

2012 ◽  
Vol 6 (3) ◽  
pp. 767-774 ◽  
Author(s):  
Wenxiao Jiang ◽  
Zhanhui Wang ◽  
Greta Nölke ◽  
Jing Zhang ◽  
Lanlan Niu ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Immunosorbent Assay ◽  
Aflatoxin B1 ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Food Matrices

Screening of aflatoxin M1, a metabolite of aflatoxin B1 in human urine samples in Malaysia: A preliminary study

Food Control ◽  
2012 ◽  
Vol 28 (1) ◽  
pp. 55-58 ◽  
Author(s):  
Mohd Redzwan Sabran ◽  
Rosita Jamaluddin ◽  
Mohd Sokhini Abdul Mutalib
Keyword(s):  
Aflatoxin B1 ◽  
Human Urine ◽  
Urine Samples ◽  
Aflatoxin M1 ◽  
Human Urine Samples ◽  
Preliminary Study

scholarly journals Comparison of two Analytical Methods (ELISA and LC-MS/MS) for Determination of Aflatoxin B1 in Corn and Aflatoxin M1 in Milk

2015 ◽  
Vol 5 ◽  
pp. 270-273 ◽  
Author(s):  
Srdjan Stefanovic ◽  
Danka Spiric ◽  
Radivoj Petronijevic ◽  
Jelena Nedeljkovic Trailovic ◽  
Dragan Milicevic ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Analytical Methods ◽  
Aflatoxin M1

scholarly journals Determination of Aflatoxin M1 in Pasteurized Liquid and Powdered Milk Products Imported to Iran

2019 ◽  
Vol 13 (2) ◽  
pp. 19-23
Author(s):  
Masoumeh Mahmoodi Maymand ◽  
◽  
Mansooreh Mazaheri ◽  
Mahboobeh Talebi Mehrdar ◽  
◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Adverse Effects ◽  
Aflatoxin B1 ◽  
Hplc Method ◽  
Aflatoxin M1 ◽  
Economic Losses ◽  
Powdered Milk ◽  
Milk Products ◽  
Growth Development

Background: Mycotoxins are the secondary metabolites of molds and have adverse effects on humans, animals, and crops, resulting in illnesses and economic losses. Aflatoxin M1 (AFM1) is a hepatocarcinogen found in the milk from animals that have consumed feeds contaminated with aflatoxin B1 (AFB1). Milk is a highly nutritious food and is a source of necessary macro- and micro-nutrients for the growth, development and maintenance of human health. Methods: The presence of AFM1 was investigated in 70 samples of imported pasteurized and powdered milk products available to the Iranian consumers. The level of AFM1 was determined by HPLC method equipped with immunoaffinity cleanup. Results: The results showed that 32% of the analyzed samples were positive for AFM1 at 0.05-3.31 μg/kg. Also, 16% of analyzed samples were positive for AFM1at concentrations higher than the limit permitted by the Iranian standards. Conclusion: The detection of AFM1 contamination in the analyzed samples indicates the importance of the health of animal feeds. Thus, monitoring the imported feed materials, especially those arriving at Iranian borders is crucial in the prevention of AFM1 and AFB1 contaminations spreading across the domestic market.

scholarly journals An Impedance Based Electrochemical Immunosensor for Aflatoxin B1 Monitoring in Pistachio Matrices

Chemosensors ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 121
Author(s):  
Michail D. Kaminiaris ◽  
Sophie Mavrikou ◽  
Maria Georgiadou ◽  
Georgia Paivana ◽  
Dimitrios I. Tsitsigiannis ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Limit Of Detection ◽  
Electrochemical Immunosensor ◽  
Aflatoxin M1 ◽  
Biomolecular Recognition ◽  
Effective Prevention ◽  
Upper Limits ◽  
Ethylcarbodiimide Hydrochloride ◽  
Food And Feed

Aflatoxins are highly toxic fungal secondary metabolites that often contaminate food and feed commodities. An electrochemical immunosensor for the determination of aflatoxin B1 (AFB1) was fabricated by immobilizing monoclonal AFB1 antibodies onto a screen-printed gold electrode that was modified with carbo-methyldextran by N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide cross-linking. An electrochemical interfacial modelling of biomolecular recognition was suggested and reasonably interpreted. Impedance technology was employed for the quantitative determination of AFB1. The limit of detection concentration of AFB1 for standard solutions and spiked pistachio samples was 0.5 ng/mL and 1 ng/mL, respectively. The immunosensor was able to successfully determine AFB1 concentrations in the range of 4.56–50.86 ng/mL in unknown pistachio samples. Comparative chromatographic analysis revealed that AFB1 concentrations that were higher than 345 ng/mL were not within the immunosensor’s upper limits of detection. Selectivity studies against Ochratoxin A and Aflatoxin M1 demonstrated that the proposed AFB1 immunosensor was able to differentiate between these other fungal mycotoxins. The novel electrochemical immunosensor approach has the potential for rapid sample screening in a portable, disposable format, thus contributing to the requirement for effective prevention and the control of aflatoxin B1 in pistachios.

scholarly journals Determination of aflatoxins in food products by the ELISA method

2013 ◽  
Vol 19 (No. 1) ◽  
pp. 8-12 ◽  
Author(s):  
J. Leszczyńska ◽  
J. MasŁowska ◽  
A. Owczarek ◽  
U. Kucharska
Keyword(s):  
Aflatoxin B1 ◽  
Dairy Products ◽  
Total Content ◽  
Food Products ◽  
Aflatoxin M1 ◽  
Elisa Method ◽  
Cereal Samples

To determine the total content of aflatoxins, aflatoxin B1 and aflatoxin M1 in food the ELISA method was used. Milk, dairy products and cereal samples were mainly investigated. A few samples were found to be contaminated with aflatoxins. A great usability of the ELISA method for aflatoxin determination in food was established. Selectivity and sensitivity of the method is reported.

Extraction, Cleanup, and Quantitative Determination of Aflatoxins B1 and M1 in Beef Liver

1979 ◽  
Vol 62 (5) ◽  
pp. 1080-1082
Author(s):  
Mary W Trucksess ◽  
Leonard Stoloff
Keyword(s):  
Citric Acid ◽  
Thin Layer ◽  
Quantitative Determination ◽  
Aflatoxin B1 ◽  
Aflatoxin M1 ◽  
Animal Tissues ◽  
Beef Liver ◽  
Layer Chromatography ◽  
Extracting Solvent

Abstract A method for the determination of aflatoxin Bx in eggs was applicable for aflatoxin B1 in liver, but ineffective for aflatoxin Mx in liver because of poor recovery of added aflatoxin and interferences in thin layer chromatography. The method was modified by the addition of citric acid to the extracting solvent and ammonium sulfate to the extract solution for removing protein. The elution system for silica gel column cleanup was also changed by substituting methanol for acetone, and adding a step for confirmation of aflatoxin M1 identity. The method has been used successfully for survey and research on aflatoxin residues in animal tissues.

Correlation between aflatoxin M1 content of breast milk, dietary exposure to aflatoxin B1 and socioeconomic status of lactating mothers in Ogun State, Nigeria

2013 ◽  
Vol 56 ◽  
pp. 171-177 ◽  
Author(s):  
Oloyede Adejumo ◽  
Olusegun Atanda ◽  
Assunta Raiola ◽  
Yinka Somorin ◽  
Ranajit Bandyopadhyay ◽  
...  
Keyword(s):  
Socioeconomic Status ◽  
Breast Milk ◽  
Aflatoxin B1 ◽  
Dietary Exposure ◽  
Aflatoxin M1 ◽  
Ogun State ◽  
Lactating Mothers

scholarly journals Determination of Aflatoxin M1 concentration in volunteers from rural and urban areas of Cochabamba-Bolivia

Bionatura ◽  
2019 ◽  
Vol 02 (Bionatura Conference Serie) ◽  
Author(s):  
Andrea Condori Vincenti ◽  
Stefania Torrico Torrico ◽  
Tania Alba Medrano ◽  
Yesica Nina Guaravia ◽  
Nora Medrano-Mercado ◽  
...  
Keyword(s):  
Human Health ◽  
Urban Areas ◽  
Aflatoxin Contamination ◽  
Urine Samples ◽  
Aflatoxin M1 ◽  
Elisa Kit ◽  
Rural And Urban Areas ◽  
Rural And Urban ◽  
Toxigenic Strains

Aflatoxins are produced by toxigenic strains of Aspergillus fungi and include the subtypes: B1, B2, G1, and G2. Once ingested, aflatoxin is metabolized to aflatoxin M1 found in the urine. Because of the importance of aflatoxins on human health, we analyzed 550 urine samples collected from farmers and city volunteers from Cochabamba. The level of aflatoxin M1 was determined using the Helica Aflatoxin M1 ELISA Kit specific for urine. 216 volunteer farmers had an aflatoxin M1 range of 0.02-1.21 ng/ml, while 334 urine city volunteers had a range of 0.03-1.27 ng/ml, indicating a risk for exposure to aflatoxin contamination for both populations.

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