Isolation and characterization of human pathogenic multidrug resistant bacteria associated with plastic litter collected in Zanzibar

2021 ◽  
Vol 405 ◽  
pp. 124591
Author(s):  
Farah N. Rasool ◽  
Mariana A. Saavedra ◽  
Siajali Pamba ◽  
Vonica Perold ◽  
Aviti J. Mmochi ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Isolation And Characterization

Isolation and characterization of multidrug-resistant bacteria from minced meat in Austria

2014 ◽  
Vol 44 ◽  
pp. 41-46 ◽  
Author(s):  
Christian Petternel ◽  
Herbert Galler ◽  
Gernot Zarfel ◽  
Josefa Luxner ◽  
Doris Haas ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Isolation And Characterization ◽  
Minced Meat

scholarly journals Isolation and Characterization of Two Klebsiella pneumoniae Phages Encoding Divergent Depolymerases

2020 ◽  
Vol 21 (9) ◽  
pp. 3160 ◽  
Author(s):  
Pilar Domingo-Calap ◽  
Beatriz Beamud ◽  
Lucas Mora-Quilis ◽  
Fernando González-Candelas ◽  
Rafael Sanjuán
Keyword(s):  
Klebsiella Pneumoniae ◽  
Multidrug Resistant ◽  
Health Concern ◽  
Resistant Bacteria ◽  
Isolation And Characterization ◽  
The Family ◽  
Important Challenge ◽  
Tail Spike ◽  
Reference Strains

The emergence of multidrug-resistant bacteria is a major global health concern. The search for new therapies has brought bacteriophages into the spotlight, and new phages are being described as possible therapeutic agents. Among the bacteria that are most extensively resistant to current antibiotics is Klebsiella pneumoniae, whose hypervariable extracellular capsule makes treatment particularly difficult. Here, we describe two new K. pneumoniae phages, πVLC5 and πVLC6, isolated from environmental samples. These phages belong to the genus Drulisvirus within the family Podoviridae. Both phages encode a similar tail spike protein with putative depolymerase activity, which is shared among other related phages and probably determines their ability to specifically infect K. pneumoniae capsular types K22 and K37. In addition, we found that phage πVLC6 also infects capsular type K13 and is capable of striping the capsules of K. pneumoniae KL2 and KL3, although the phage was not infectious in these two strains. Genome sequence analysis suggested that the extended tropism of phage πVLC6 is conferred by a second, divergent depolymerase. Phage πVLC5 encodes yet another putative depolymerase, but we found no activity of this phage against capsular types other than K22 and K37, after testing a panel of 77 reference strains. Overall, our results confirm that most phages productively infected one or few Klebsiella capsular types. This constitutes an important challenge for clinical applications.

scholarly journals Structural characterization of scorpion peptides and their bactericidal activity against clinical isolates of multidrug-resistant bacteria

PLoS ONE ◽  
2019 ◽  
Vol 14 (11) ◽  
pp. e0222438 ◽  
Author(s):  
Catherine Cesa-Luna ◽  
Jesús Muñoz-Rojas ◽  
Gloria Saab-Rincon ◽  
Antonino Baez ◽  
Yolanda Elizabeth Morales-García ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Bactericidal Activity ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria

scholarly journals Isolation and Characterization of Multidrug Resistant Enterobacteriaceae in Urine Sample of Patients Suffering from Urinary Tract Infection with Diabetes and Nephropathy

2021 ◽  
Vol 20 (1) ◽  
pp. 87-93
Author(s):  
Nazmul Ahsan ◽  
Monzilur Rahman ◽  
Md Nazrul Islam ◽  
Anwarul Azim Akhand
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Diabetic Patients ◽  
Isolation And Characterization ◽  
Alternative Drug ◽  
Enterobacter Asburiae

Multidrug-resistant (MDR) organisms are spreading widely and becoming an issue of utmost importance to deal with. In the current study, ten urine samples from diabetic patients suffering from multiple complications, including urinary tract infection (UTI) and nephropathy were investigated. Antibiogram assays of the bacterial isolates from collected samples demonstrated resistance against most of the antibiotics tested. Further studies were conducted to determine the types of resistant bacteria that caused UTI. Analyzing the 16S rDNA sequence and phylogenetic tree, 3 isolates were identified as Escherichia coli, 5 as Klebsiella pneumoniae and the rest 2 as Enterobacter asburiae. The findings of this research indicate the necessity of urgent attention to find an effective alternative drug for treating infections caused by these resistant isolates. Dhaka Univ. J. Pharm. Sci. 20(1): 87-93, 2021 (June)

scholarly journals Pattern of antibiotics resistance and phenotypic characterization of Multidrug resistant bacteria isolates in four hospitals of Littoral region, Cameroon

2021 ◽  
Vol 11 (1-s) ◽  
pp. 20-30
Author(s):  
Merlin Jonas Wandji Takemegni ◽  
Assob Nguedia Jules clement ◽  
Ateudjieu Jérôme ◽  
Enow Orock George ◽  
Mbopi-Keou Francois-Xavier ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Susceptibility Test ◽  
Diffusion Method ◽  
Phenotypic Characterization ◽  
Military Hospital ◽  
Resistant Bacteria ◽  
Antibiotics Resistance ◽  
Multidrug Resistant Bacteria ◽  
Littoral Region

Objective: To describe the pattern of antibiotics resistance and phenotypic characterization of Multidrug resistant bacteria isolates in four hospitals of Littoral region, Cameroon. Methods: We conducted a descriptive hospital based cross-sectional study from December 2018 to May 2019. A simple random sampling was used to swap 10 selected equipment and 10 materials in the mornings after disinfection but before the start of work in seven units. After inoculation in four agar media consecutively (Eosine Metyleine blue, Cled, Manitol salt agar and blood agar ) and incubated in appropriate conditions, the Kirby-Bauer disk-diffusion method was used for antimicrobial susceptibility test. Results: Among 50.4% (119/236) showed positive bacteria growth, a total of 89 (13 species), predominant bacteria and those more likely to cause nosocomial infections were selected and tested each one to 18 antibiotics. There was high level of resistance to Penicillin (amoxicillin (77.5%) and Oxacillin (76.4%)), followed by 3G Cephalosporine (Ceftazidime (74.2%)) and Monobactam (Aztreonam (70.8%)). Although the least level of resistance was observed in Carbapenem (imipenem (5.6%)). The overall prevalence of MDRB was 62.9% (56/89). MRSA were the mostly detected 57.5 % (30/89), followed by ESBL 10.1% (9/89). Military hospital of Douala and Emergency unit was the MDRBs dominantly contaminated area respectively 39.3% (22/56) and 17.9% (10/56). Conclusion: MDRB occurred to be a current public health problem as well as hospital surfaces are worrying reservoir that can be spread to patient, health professionals and visitors. Keywords: Antibiotic resistance, susceptibility test, multidrug-resistant bacteria, Hospital facilities, Units, Littoral Region-Cameroon

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