Uvariopsis dicaprio (Annonaceae) a new tree species with notes on its pollination biology, and the Critically Endangered narrowly endemic plant species of the Ebo Forest, Cameroon

Background The Ebo Forest area is a highly threatened centre of diversity in the Littoral Region of Cameroon, globally important for conservation with many threatened species including 68 threatened species of plant, yet not formally protected. The tropical African evergreen forest tree genus Uvariopsis Engl. & Diels (Annonaceae) is characterised by unisexual, usually cauliflorous flowers with a uniseriate corolla of four petals, and two sepals. Cameroon is the centre of diversity of the genus with 14 of the 19 known species. Methods The herbarium collection MacKinnon 51 from Ebo is hypothesized to represent a new species to science of Uvariopsis. This hypothesis is tested by the study of herbarium specimens from a number of herbaria known to hold important collections from Cameroon and surrounding countries. Results We test the hypothesis that MacKinnon 51 represents a new species to science, using the most recent dichotomous identification key, and comparing it morphologically with reference material of all known species of the genus. We make a detailed comparative morphological study focussing on three other Cameroonian species, Uvariopsis solheidii, U. korupensis and the sympatric U. submontana. In the context of a review of the pollination biology of Uvariopsis, we speculate that in a genus otherwise with species with dull, flesh-coloured (pink, red to brown) flowers pollinated (where known) by diptera, orthoptera and blattodea (flies, crickets and cockroaches), the glossy, pale yellow-green flowers of Uvariopsis dicaprio, with additional traits unique in the genus, may be pollinated by nocturnal moths. Based on MacKinnon 51, we formally name Uvariopsis dicaprio Cheek & Gosline (Annonaceae) as new to science, and we describe, and illustrate, and map it. Restricted so far to a single site in evergreen forest in the Ebo Forest, Littoral Region, Cameroon, Uvariopsis dicaprio is provisionally assessed as Critically Endangered using the IUCN, 2012 standard because the forest habitat of this species remains unprotected, and there exist imminent threats of logging and conversion to plantations. Discussion We show that the highest density of species of the genus (12), and of narrow endemics (5), is found in the Cross-Sanaga Interval of SE Nigeria and Western Cameroon. A revised key to the 14 Cameroonian species of Uvariopsis is presented. We review the other seven narrowly endemic and threatened species unique to the Ebo forest of Cameroon and discuss the phytogeographic affinities of the area. Conclusions Uvariopsis dicaprio adds to the growing list of species threatened with extinction at Ebo Forest due to current anthropogenic pressures.

Predictors of Drug-Resistant Tuberculosis among High-Risk Population Diagnosed under National Program Conditions in the Littoral Region, Cameroon

Multiple drug resistance TB (MDR-TB) has greatly jeopardized the effective control of tuberculosis in Africa. This study is aimed at determining the incidence and predictors of drug resistant-TB amongst bacteriologically diagnosed cases in the Littoral region of Cameroon. This was a descriptive cross-sectional survey conducted from January 2016 to December 2017. A total of 1665 participants were enrolled from 32 diagnostic and treatment centers (DTCs) in the Littoral region. Demographic, clinical, socioeconomic, and behavioral data were obtained using a pretested structured questionnaire. Drug susceptibility testing was performed using Gene Xpert MTB/RIF assay and line probe assay (LPA). Consent was obtained from participant/guidance. Data analysis was carried with SPSS version 21. Univariate and multiple logistic regression was performed at 5% significance level. The incidence of rifampicin and MDR-TB was 86 (5.2%) and 75 (4.5%), respectively. More (11.3%) cases of drug resistance were diagnosed in 2016 compared to 2017 (3.7%). Eleven (0.7%) were resistant to rifampicin only. A total of 19 (4.4%) cases of rifampicin resistance were detected from newly diagnosed cases and 67 (5.4%) from previously retreated cases. Pre-XDR-TB was detected in 2 (2.7%) of the MDR-TB cases amongst whom 1 (1.3%) was extensive drug resistance TB (XDR-TB). Age greater than 60 years old ( OR = 4.98 , p = 0.047 ), being married ( OR = 1.91 , p = 0.006 ), being currently incarcerated ( OR = 1.74 , p = 0.027 ), and having contact with known TB cases ( OR = 1.88 , p = 0.007 ) were associated to MDR-TB in a univariate analysis. This study highlights the declining rates of TB drug resistance in the region over the years probably due to the introduction of Gene Xpert that results in early detection of RR-TB. It also shows that age greater than 60 years, being married, and incarcerated are predictors of drug resistant-TB, while the year of patient enrolment and previous exposure to TB treatment were independent predictors of drug resistance in the Littoral region of Cameroon.

Four new threatened species of Rinorea (Violaceae), treelets from the forests of Cameroon

SummaryFour species of Rinorea are described as new to science; all four species are endemic to evergreen rain forest in Cameroon. Rinorea villiersii Achound. and R. amietii Achound are placed in Rinorea [unranked] Crassiflorae M.Brandt, while, R. dewildei Achound. and R. faurei Achound. fall in Rinorea [unranked] Dentatae Engl. The first species appears to be endemic to the Solé Forest Reserve northeast of Yabassi in Littoral Region. The second and the third species are found mainly in Littoral and South Regions, R. amietii extending to South West Region and R. dewildei extending to Central Region. The fourth species, R. faurei is an endemic of the Santchou Forest Reserve at the foot of Dschang Plateau in West Region. The taxonomic affinities of the four species are discussed, illustrations and a distribution map are provided, and their conservation status is assessed. All four species are threatened with extinction according to the 2012 IUCN categories and criteria.

Kupeantha yabassi (Coffeeae-Rubiaceae), a new Critically Endangered shrub species of the Ebo Forest area, Littoral Region, Cameroon

SummaryA new species to science of evergreen forest shrub, Kupeantha yabassi M.G.Alvarez & Cheek (Coffeeae - Rubiaceae), is described, illustrated and compared morphologically with the closely similar species K. pentamera. Restricted so far to a single site in evergreen lowland forest near the Ebo Forest, Yabassi, Littoral Region, Cameroon, this species is Critically Endangered using the IUCN 2012 standard due to habitat clearance driven mainly by agriculture, adding to the growing list of threatened species resulting from anthropogenic pressure on Cameroon forests. A revised key to the six species of Kupeantha is presented. Two distinct geographical and ecological species groupings within the genus are identified and discussed. Notes are given on other narrowly endemic and threatened species in the Ebo Forest area, a threatened centre of diversity important for conservation in the Littoral Region.

Matrix of Architectural Solutions for the Conflict between Transport Infrastructures, Landscape and Urban Habitat along the Mediterranean Coastline: The Case of the Maresme Region in Barcelona, Spain

Maresme is a littoral region of Barcelona (Spain) in which the railway and an important road run along the coastline with a high landscape impact. Over time, several facilities connected to these transport infrastructures have appeared, such as industries, malls, marinas or train stations. These activities profit from the easy connection but create a barrier between the inhabitants and the sea. This research follows three aspects identified in a large variety of locations along the Mediterranean coast: longitudinal mobility, transversal accessibility and landscape discontinuities. The first territorial analysis defines a series of urban problematics classified by category. Then, the most representative case studies are developed by means of urban and architectural projects. The comparative analysis of these proposals provides a catalogue of design strategies which can be combined as criteria for solving multiple conflicts detected in the region. The result of this project is a methodology based on a matrix of general guidelines to ease the solving of local conflicts in a homogeneous way for the whole territory. The final aim is to re-establish order and continuity in the Mediterranean littoral skyline, fostering sustainable mobility and recovering public space for inhabitants.

Efficacy of Methanolic and Aqueous Extracts of Thevetia peruviana (pers.) K. Schum on Growth of Phytophthora colocasiae Racib, Causal Agent of Taro Late Blight in Cameroon

This study aims to evaluate the antifungal activities of methanolic and aqueous extracts of Thevetia peruviana seeds on the in vitro growth of Phytophthora colocasiae. A randomized sample block design containing four treatments (T-: absolute control, AE, ME and Callomil Plus at the dose of 12.5 μL/ml) with three repetitions was used. Plant extracts were used at three concentrations: C1: 12.5 µl/ml; C2: 25 µl/ml and C3: 50 µl/ml. The study took place in the University of Yaoundé 1, Faculty of Sciences, Department of Plant Biology, Laboratory of Phytopathology and Crop Protection, and in the Institute of Agricultural Research for Development (IARD) of Yaoundé, Laboratory of Phytopathology, during the year 2019-2020. Aqueous and methanolic extracts of T. peruviana were prepared and used at the concentrations of 12.5, 25 and 50 μL/ml. P. colocasiae was isolated from infected taro leaf cultivars "Macumba/Ibo coco" located in three different regions in Cameroon: West, Littoral and Centre. The different leaf explants of taro were put in V8 agar medium and maintained in pure culture. Mycelial fragments of P. colocasiae of about 0.8 cm in diameter were cut and placed in sterile Petri dishes containing Potato Dextrose Agar (PDA) medium supplemented with different concentrations of plant extracts and incubated at 23±1°C for seven days for the evaluation of the radial growth. Methanolic and aqueous extracts have completely inhibited the growth of West and Littoral strains at 25 μL/mL while total inhibition of the pathogen was not obtained with strain of Centre region. The lowest inhibition was obtained with the strain of Centre region (85.1%) for aqueous extract and (70.95%) for methanolic extract compare to 100% for West and Littoral region at highest concentration. The aqueous extract at the concentration of 25 μL/ml totally inhibited the in vitro radial growth of some strains of P. colocasiae. This extract, active against P. colocasiae could be used as alternative to fungicides for the control of taro leaf blight. In other hand, the strain of Littoral region was most sensible to extracts than the others. This strain could be used to provide a genetic resource for future trials in natural conditions in greenhouse and in the field.

Assessment of the quality of effluent management from university hospitals in the Littoral department in Benin

Background Liquid discharges from hospitals (effluents) threaten the environment and are now a central concern of all stakeholders in the health system and those in the protection of the environment. The management of effluents is a major problem in developing countries. The objective of this study was to assess the quality of effluent management at the level of university hospital centers (CHU) in the Littoral region in Benin. Methods It was a cross-sectional, descriptive, evaluative study that took place in 2020 to assess the “structure”, “process” and “results” components according to standard thresholds (Bad: < 60%; Acceptable: [60–80% [and Good: ≥ 80%). Results In all the CHUs, all the components, as well as the overall quality of the management of hospital effluents, had a score between 0 and 60%, with an assessment deemed bad. The poor quality of the process highlighted the non-compliance with standards relating to the management of hospital liquid discharges. Several factors linked to the “structure”, “process” and “results” components at the same time explain this poor management of university hospitals effluents. Conclusion These effluents discharged without prior treatment into wastewater could constitute a source of dissemination of potentially pathogenic microorganisms. It is therefore important to develop methods for treating these effluents before they are released into the natural environment.