351 The circular RNA circGLIS3 activates dermal fibroblasts and increases ECM production to improve wound healing

AbstractInspired by the effectiveness of low-intensity ultrasound on tissue regeneration, we investigated the potential effect of short-term high-intensity ultrasound treatment for acceleration of wound healing in an in vitro wound model and dermal equivalent, both comprising human dermal fibroblasts. Short-term ultrasound of various amplitudes significantly increased the proliferation and migration of fibroblasts and subsequently increased the production of the extracellular matrix components fibronectin and collagen type I, both of which are important for wound healing and are secreted by fibroblasts. In addition, ultrasound treatment increased the contraction of a fibroblast-embedded three-dimensional collagen matrix, and the effect was synergistically increased in the presence of TGF-β. RNA-sequencing and bioinformatics analyses revealed changes in gene expression and p38 and ERK1/2 MAPK pathway activation in the ultrasound-stimulated fibroblasts. Our findings suggest that ultrasound as a mechanical stimulus can activate human dermal fibroblasts. Therefore, the activation of fibroblasts using ultrasound may improve the healing of various types of wounds and increase skin regeneration.

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Wound Healing Promotion by Hyaluronic Acid: Effect of Molecular Weight on Gene Expression and In Vivo Wound Closure

Pharmaceuticals ◽

10.3390/ph14040301 ◽

2021 ◽

Vol 14 (4) ◽

pp. 301

Author(s):

Yayoi Kawano ◽

Viorica Patrulea ◽

Emmanuelle Sublet ◽

Gerrit Borchard ◽

Takuya Iyoda ◽

...

Keyword(s):

Gene Expression ◽

Molecular Weight ◽

Wound Healing ◽

Hyaluronic Acid ◽

Healing Process ◽

Dermal Fibroblasts ◽

Water Soluble ◽

Wound Healing Process ◽

And Migration ◽

Proliferation And Migration

Hyaluronic acid (HA) has been known to play an important role in wound healing process. However, the effect of molecular weight (MW) of exogenously administered HA on the wound healing process has not been fully understood. In this study, we investigated HA with different MWs on wound healing process using human epidermal keratinocytes and dermal fibroblasts. Cell proliferation and migration ability were assessed by water soluble tetrazolium (WST) assay and wound scratch assay. We examined the effect of HA addition in a full-thickness wound model in mice and the gene expression related to wound healing. Proliferation and migration of HaCaT cells increased with the increase of MW and concentration of HA. Interleukin (IL-1β), IL-8 and vascular endothelial growth factor (VEGF) as well as matrix metalloproteinase (MMP)-9 and MMP-13 were significantly upregulated by high molecular weight (HMW) HA in keratinocytes. Together with VEGF upregulation and the observed promotion of HaCaT migration, HA with the MW of 2290 kDa may hold potential to improve re-epithelialization, a critical obstacle to heal chronic wounds.

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Galvanic microparticles increase migration of human dermal fibroblasts in a wound-healing model via reactive oxygen species pathway

Experimental Cell Research ◽

10.1016/j.yexcr.2013.09.016 ◽

2014 ◽

Vol 320 (1) ◽

pp. 79-91 ◽

Cited By ~ 14

Author(s):

Nina Tandon ◽

Elisa Cimetta ◽

Aranzazu Villasante ◽

Nicolette Kupferstein ◽

Michael D. Southall ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Wound Healing ◽

Reactive Oxygen ◽

Dermal Fibroblasts ◽

Oxygen Species ◽

Human Dermal Fibroblasts ◽

Wound Healing Model ◽

Healing Model

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Modulating senescent cells to improve wound healing with age

Cytotherapy ◽

10.1016/s1465324921005193 ◽

2021 ◽

Vol 23 (5) ◽

pp. S147

Author(s):

U. Niyogi ◽

M.J. Ouellette ◽

M.A. Carlson

Keyword(s):

Wound Healing ◽

Improve Wound Healing

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Foslip®-based photodynamic therapy as a means to improve wound healing

Photodiagnosis and Photodynamic Therapy ◽

10.1016/j.pdpdt.2011.06.003 ◽

2011 ◽

Vol 8 (4) ◽

pp. 321-327 ◽

Cited By ~ 22

Author(s):

Julie Garrier ◽

Lina Bezdetnaya ◽

Catherine Barlier ◽

Susanna Gräfe ◽

François Guillemin ◽

...

Keyword(s):

Wound Healing ◽

Photodynamic Therapy ◽

Improve Wound Healing

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Surgical Suture Assembled with Tadalafil/Polycaprolactone Drug-Delivery for Vascular Stimulation Around Wound: Validated in a Preclinical Model

Biointerface Research in Applied Chemistry ◽

10.33263/briac105.63176327 ◽

2020 ◽

Vol 10 (5) ◽

pp. 6317-6327 ◽

Cited By ~ 1

Keyword(s):

Wound Healing ◽

Tensile Strength ◽

Drug Release ◽

Study Groups ◽

Preclinical Model ◽

Surgical Suture ◽

Electrospinning Method ◽

Hematoxylin And Eosin ◽

Improve Wound Healing

In this study, a novel Polycaprolactone suture assembled with Tadalafil was investigated to improve wound healing processes via vascular stimulation. Tadalafil/Polycaprolactone (TP) suture was developed by the electrospinning method. The designed suture was characterized by SEM, mechanical properties assessments, tensile strength measurements and the drug release study. For in vivo tests, rats were classified into two study groups. An incision was made on their back skin and they were sutured with TP suture and Polycaprolactone suture as control. Rats were sacrificed at 7 days following surgery for histopathological examinations with Hematoxylin and Eosin staining. Results of Tensile test demonstrated that the lowest tensile strength belonged to 3 and 4 % wt and the highest tensile strength belonged to 1 and 2 % wt of TP suture. The rate of Tadalafil release showed that the highest drug release was related to 3 and 4% wt which were about 125 to 210 μg during 15 days. The histopathology revealed that the number of blood vessels, collagen fibers, fibroblast, polymorphonuclear leukocytes, and epithelization was remarkable in Tadalafil/Polycaprolactone group during 7-day. A novel Tadalafil/Polycaprolactone suture improved the processes of wound healing by releasing the Tadalafil drug around the sutured wound and can be used in medical applications.

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Circular RNA expression profiles significantly altered in UVA‑irradiated human dermal fibroblasts

Experimental and Therapeutic Medicine ◽

10.3892/etm.2020.9292 ◽

2020 ◽

Vol 20 (6) ◽

pp. 1-1

Author(s):

Mengbi Lin ◽

Yue Zheng ◽

Qian Li ◽

Yufang Liu ◽

Qingfang Xu ◽

...

Keyword(s):

Expression Profiles ◽

Circular Rna ◽

Dermal Fibroblasts ◽

Rna Expression ◽

Human Dermal Fibroblasts

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Asiaticoside induces cell proliferation and collagen synthesis in human dermal fibroblasts

Zinc supplementation improves heme biosynthesis in rats exposed to lead ◽

10.18051/univmed.2015.v34.96-103 ◽

2015 ◽

Vol 34 (2) ◽

pp. 96

Author(s):

Linda Yuliati ◽

Etik Mardliyati ◽

Kusmarinah Bramono ◽

Hans Joachim Freisleben

Keyword(s):

Cell Proliferation ◽

Wound Healing ◽

Retinoic Acid ◽

Collagen Synthesis ◽

Active Agent ◽

Dermal Fibroblasts ◽

Type Iii Collagen ◽

Type I ◽

Human Dermal Fibroblasts ◽

Type Iii

Background Asiatiocoside, a saponin component isolated from Centella asiatica can improve wound healing by promoting the proliferation of human dermal fibroblasts (HDF) and synthesis of collagen. The skin-renewing cells and type I and III collagen synthesis decrease with aging, resulting in the reduction of skin elasticity and delayed wound healing. Usage of natural active compounds from plants in wound healing should be evaluated and compared to retinoic acid as an active agent that regulates wound healing. The aim of this study was to compare and evaluate the effect of asiaticoside and retinoic acid to induce greater cell proliferation and type I and III collagen synthesis in human dermal fibroblast. Methods Laboratory experiments were conducted using human dermal fibroblasts (HDF) isolated from human foreskin explants. Seven passages of HDF were treated with asiaticoside and retinoic acid at several doses and incubated for 24 and 48 hours. Cell viability in all groups was tested with the MTT assay to assess HDF proliferation. Type I and III collagen synthesis was examined using the respective ELISA kits. Analysis of variance was performed to compare the treatment groups. Results Asiaticoside had significantly stronger effects on HDF proliferation than retinoic acid (p<0.05). The type III collagen production was significantly greater induction with asiaticoside compared to retinoic acid (p<0.05). Conclusion Asiaticoside induces HDF proliferation and type I and III collagen synthesis in a time- and dose-dependent pattern. Asiaticoside has a similar effect as retinoic acid on type I and type III collagen synthesis.

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