Comparison of ADAMTS Levels in Pulp Tissue Samples of Healthy and Symptomatic Irreversible Pulpitis Teeth

2021 ◽  
Author(s):  
H.F. Golbasi ◽  
A. Erdemir ◽  
U. Kisa
Keyword(s):  
Pulp Tissue ◽  
Tissue Samples ◽  
Irreversible Pulpitis

scholarly journals The Effect of Zingiber officinale on the Spleen Tissue and Antibody Titer of Broiler Chickens

2019 ◽  
Vol 36 (01) ◽  
pp. 046-050
Author(s):  
Alireza Taghdisi ◽  
Sajjad Hejazi
Keyword(s):  
Broiler Chickens ◽  
Zingiber Officinale ◽  
Control Group ◽  
White Pulp ◽  
Broiler Chicks ◽  
Pulp Tissue ◽  
Tissue Samples ◽  
Serum Factors ◽  
Spleen Tissue ◽  
Significant Difference

Introduction Increasing the immune system's function of fighting infectious diseases is very important in the poultry industry. Ginger, scientifically known as Zingiber officinale, belongs to the Zingiberaceae family. The use of ginger in the diet of poultry increases serum levels of superoxide dismutase enzymes and glutathione peroxidase, which are considered to be important antioxidant enzymes. The main objective of the present study is to evaluate the effect of ginger on the spleen tissue of broiler chickens. Material and Methods The specimens comprised 2 groups of 20 Ross breed broiler chicks, for 42 days and were then, examined and tested. The diet was supplemented with 1 g/kg of ginger powder from the beginning of the rearing period. Blood samples of the chicks were randomly collected to measure the levels of hemagglutination (HI). The removed spleens were fixed with 10% formalin buffer. The specimens were cut in 5-micron diameters and stained with hematoxylin and eosin. Results and Conclusion There was a statistically significant difference in the mean of HI blood titers between the chicks in the growth period and final period groups (p < 0.05). The white-pulp tissue samples were more clearly seen in the treatment group than in the control group, and also, it was observed that the wall of the central artery of the white pulp was thicker in the ginger-treated group as compared with the control group. The nutritional value of ginger may vary. Thus, it is necessary to investigate the effect of this plant final on weight gain; the serum factors associated with the metabolic chart, and the response of the immune system to this plant.

scholarly journals Biodentine™ Partial Pulpotomy of a Young Permanent Molar with Signs and Symptoms Indicative of Irreversible Pulpitis and Periapical Lesion: A Case Report of a Five-Year Follow-Up

2019 ◽  
Vol 2019 ◽  
pp. 1-5
Author(s):  
W. Chinadet ◽  
T. Sutharaphan ◽  
P. Chompu-inwai
Keyword(s):  
Signs And Symptoms ◽  
Carious Lesion ◽  
Periapical Lesion ◽  
Pulp Tissue ◽  
Permanent Molar ◽  
Exposure Site ◽  
Patient Reported ◽  
Periapical Healing ◽  
Irreversible Pulpitis

The purpose of this paper was to report the five-year success of Biodentine™ partial pulpotomy in a young permanent molar, with signs and symptoms indicative of irreversible pulpitis and periapical lesion, in a nine-year-old girl. Preoperative clinical examination revealed a large carious lesion of the left mandibular permanent first molar. The patient reported pain on percussion. The tooth responded positively to the electric pulp test and had lingering pain after cold testing. A periapical radiograph showed a deep carious lesion and periapical lesion. Based on the clinical and radiographical examination, the tooth had signs and symptoms indicative of irreversible pulpitis and periapical lesion. During caries removal, pulp exposure occurred, and 2-3 mm in depth of pulp tissue at the exposure site was removed. Haemorrhage was controlled within four minutes with 2.5% sodium hypochlorite-moistened cotton pellets. Biodentine™ was then applied as both a pulp dressing and a temporary restoration. At the following visit, composite resin was placed over the Biodentine™ as a final restoration. During a five-year follow-up, the tooth was asymptomatic, had positive responses to sensibility tests, and had no discolouration. Follow-up radiographs showed a dentine bridge and periapical healing.

scholarly journals Pulp tissue dissolution capacity of QMix 2in1 irrigation solution

2015 ◽  
Vol 09 (03) ◽  
pp. 423-427 ◽  
Author(s):  
Dilara Arslan ◽  
Mehmet Burak Guneser ◽  
Alper Kustarci ◽  
Kursat Er ◽  
Seyda Herguner Siso
Keyword(s):  
Saline Solution ◽  
In Vitro Study ◽  
Control Group ◽  
Pulp Tissue ◽  
Tissue Samples ◽  
Canal Irrigation ◽  
Root Canal Irrigation ◽  
Significant Difference ◽  
Group 2

ABSTRACT Objective: The aim of this study was to evaluate the tissue dissolution efficacy of four root canal irrigation solutions (sodium hypochlorite [NaOCl], chlorhexidine gluconate [CHX], Octenidine [OCT], and QMix 2in1) on bovine pulp tissue. Materials and Methods: Fifty bovine pulp tissue samples, each weighing 6.55 mg, were prepared and randomly divided into four experimental groups and one control group (n = 10) according to the dissolution irrigants used: (1) 5.25% NaOCl group; (2) 2% CHX group; (3) OCT group; (4) QMix 2in1 group; and (5) control group (saline solution). These samples were then placed into special bovine dentin reservoir models and immersed for 1 h with each test solution (0.1 mL of each) at room temperature. The pulp samples were then blotted dry and weighed again. The percentage of weight loss was calculated. Statistically analyzed with one-way analysis of variance and post-hoc Tukey tests (P = 0.05). Results: Saline solution did not dissolve the bovine pulp tissue. All groups, except OCT, dissolved pulp samples more effectively than the control group (P < 0.05). The highest tissue dissolution was observed in 5.25% NaOCl group (P < 0.05). No statistically significant difference was found between the tissue-dissolving effect between QMix 2in1 and those of 2% CHX. Conclusions: Within the limitations of this in vitro study, NaOCl exhibited the best tissue-dissolving effect out of all solutions tested. CHX and QMix 2in1 were able to dissolve pulp tissue but less than NaOCl. OCT and saline solutions could not exhibit significantly tissue-dissolving effectiveness. This study shown that QMix 2in1 has little capacity to dissolve pulp tissue therefore used alone is not sufficient for this purpose.

Eugenol Toxicity in Human Dental Pulp Fibroblasts of Primary Teeth

2016 ◽  
Vol 40 (4) ◽  
pp. 312-318 ◽  
Author(s):  
Maria Escobar-García ◽  
Karen Rodríguez-Contreras ◽  
Socorro Ruiz-Rodríguez ◽  
Mauricio Pierdant-Pérez ◽  
Bernardino Cerda-Cristerna ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Primary Teeth ◽  
Toxicity Tests ◽  
Phenazine Methosulfate ◽  
High Toxicity ◽  
Pulp Tissue ◽  
Tissue Samples ◽  
Low Concentrations ◽  
Dental Pulp Tissue ◽  
Human Dental Pulp

Objective: The aim of the study was to determine the eugenol concentrations at which toxicity occurs in human dental pulp fibroblasts of primary teeth. Study design: Samples of primary dental pulp tissue were taken. Tissue samples were seeded by means of explant technique and used in the 4th–5th pass. Single Cell Gel Electrophoresis (Comet), phenazine MeThoSulfate (MTS), LIVE/DEAD® Cell Viability/Toxicity and trypan blue assays for evaluation of the cytotoxicity of increasing concentrations of eugenol (0.06 to 810 μM) were performed. Results: The results of toxicity tests showed toxic effects on dental pulp fibroblasts, even at very low concentrations of eugenol (0.06 μM). Very low concentrations of eugenol produce high toxicity in human dental pulp fibroblasts. Conclusions: All of the concentrations of eugenol that we evaluated produced high toxicity in human dental pulp fibroblasts of primary teeth.

scholarly journals Histological Evaluation of Multisonic Technology for Debridement of Vital and Necrotic Pulp Tissues from Human Molar Teeth. An Observational Study

2021 ◽  
Vol 11 (22) ◽  
pp. 11002
Author(s):  
David E. Jaramillo ◽  
Alberto R. Arriola
Keyword(s):  
Observational Study ◽  
Root Canal ◽  
Histological Evaluation ◽  
Canal System ◽  
Pulp Tissue ◽  
Dentinal Tubules ◽  
Mandibular Molar ◽  
Molar Teeth ◽  
Root Canal System ◽  
Irreversible Pulpitis

Background: This study evaluated the efficacy of a multisonic technology for the debridement of vital and necrotic pulp tissues in freshly extracted human mandibular molar teeth. Methods: Twelve teeth with a diagnosis of symptomatic irreversible pulpitis (SIP) and twelve teeth with a diagnosis of pulp necrosis with symptomatic apical periodontitis (SAP) were extracted. The GentleWave® procedure was performed on 10 teeth from each group. Four non-treated teeth served as histologic controls. Histological consecutive 5 µm sections were obtained from the apical, middle, and coronal portion of the canals. The canals were evaluated for the presence of pulpal debris and bacteria. Results: In nine out of the ten specimens with SIP, no pulpal debris was detected in any portion of the canals. In the necrotic pulp group, eight out of the ten specimens had no detectable pulpal debris in any portion of the canal spaces. No bacteria were detected in the main canals, isthmuses, or lateral canals, but were detected deep within the dentinal tubules in 10 specimens. Conclusions: This study demonstrated that the multisonic technology was effective at removing vital and necrotic pulp tissue as well as bacteria from the root canal system, including inaccessible areas.

Clinical Value and Potential Target of miR-27a-3p in Pulpitis

2021 ◽  
pp. 1-8
Author(s):  
Ming Zhou ◽  
Chaohong Li
Keyword(s):  
Target Gene ◽  
Characteristic Curve ◽  
Luciferase Reporter ◽  
Blood Monocytes ◽  
Clinical Value ◽  
Serum Samples ◽  
Pulp Tissue ◽  
Direct Target Gene ◽  
High Level ◽  
Irreversible Pulpitis

<b><i>Introduction:</i></b> This study investigated the clinical values of miR-27a-3p for pulpitis patients, and its association with TLR4. <b><i>Methods:</i></b> Sixty-six patients with pulpitis and 34 cases without pulpitis were recruited; the pulp tissue and serum samples were collected from each participant. Real-time polymerase chain reaction was used for measurement of gene expression levels. The diagnosis values were assessed by the receiver operating characteristic curve. The target gene of miR-27a-3p was confirmed by the luciferase reporter assay. <b><i>Results:</i></b> MiR-27a-3p was downregulated in both serum and pulp tissue of pulpitis patients. MiR-27a-3p could distinguish pulpitis patients from healthy controls and might be a predictor for the development of irreversible pulpitis. A high level of TLR4 was also detected in both peripheral blood monocytes and pulp tissues from pulpitis patients and showed a negative association with the miR-27a-3p level. TLR4 was a direct target gene of miR-27a-3p. <b><i>Discussion/Conclusion:</i></b> MiR-27a-3p might be a promising biomarker for the diagnosis of pulpitis and predict the development of irreversible pulpitis. MiR-27a-3p might be involved in the pathogenesis of pulpitis via targeting TLR4.

scholarly journals Coronal Pulpotomy Technique Analysis as an Alternative to Pulpectomy for Preserving the Tooth Vitality, in the Context of Tissue Regeneration: A Correlated Clinical Study across 4 Adult Permanent Molars

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Raji Viola Solomon ◽  
Umrana Faizuddin ◽  
Parupalli Karunakar ◽  
Grandhala Deepthi Sarvani ◽  
Sevvana Sree Soumya
Keyword(s):  
Clinical Study ◽  
Permanent Teeth ◽  
Treatment Modalities ◽  
Platelet Concentrates ◽  
Pulp Tissue ◽  
Old Patients ◽  
Clinical And Radiological Outcome ◽  
Permanent Molars ◽  
Irreversible Pulpitis

Aim. (1) The aim of the clinical study revolves around the accurate diagnosis, proper case selection, and the management of acute irreversible pulpitis in permanent molars with closed apices using conservative and economical treatment modalities like vital pulpotomies with regenerative approaches over conventional root canal procedures. (2) To evaluate the use of autologous substances such as platelet concentrates and calcium silicate based materials in promoting the healing and regeneration of the inflamed pulp.Summary. Vital pulpotomy was performed on 5 carious involved, permanent molars diagnosed with acute irreversible pulpitis in 17- to 22-year-old patients. Taking into consideration the patient’s age and the condition of the underlying pulp tissue, PRF pulpotomy was planned in view of preserving the vitality of the intact radicular pulps. Regenerative procedures with second generation blood matrices were chosen to encourage the recovery of the inflamed pulps. The systematic follow-up examinations performed at 3, 6, 9, 12, 18, 22, and 24 months revealed a successful clinical and radiological outcome. Within the limits of the present clinical study and correlating the success across the treated clinical cases, we safely conclude the potential scope of regenerative pulpotomy approaches in acute irreversible pulpitis in adult permanent teeth.

scholarly journals 244 Antioxidant Activity of Opuntia Cactus Pears

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 433B-433 ◽  
Author(s):  
J.O. Kuti
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Activities ◽  
Beta Carotene ◽  
Alpha Tocopherol ◽  
Total Flavonoids ◽  
Flavonoid Content ◽  
Pear Fruit ◽  
Pulp Tissue ◽  
Tissue Samples

Flavonoid content and antioxidant activity in peel and pulp samples of four different cactus pear fruit varieties were investigated. Major cactus fruit flavonoids were quercetin, kaempferol, and isorhamnetin. Greater amount of quercetin was found in the pulp compared with the peel samples in all varieties examined. Both kaempferol and isorhamnetin were found in at least three of the varieties (Opuntia ficus-indica; O. lindheimeri; O. streptacantha) exclusively in the peel samples. Generally, pulp tissue samples of all the cactus fruit varieties contained greater ascorbic acid, glutathione, alpha-tocopherol and beta-carotene and antioxidant activities than the peel tissue samples. Total flavonoids correlated well with antioxidant activity (r2 = 0.89). Ascorbic acid had the highest antioxidant activity, followed by glutathione, beta-carotene, and alpha-tocopherol on equimolar basis.

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