Effect of ethanol extract of Portulaca oleracea L on ovulation and estrous cycle in female albino rats

2013 ◽  
Vol 6 (4) ◽  
pp. 431-436 ◽  
Author(s):  
Ramesh L. Londonkar ◽  
Hanumanatappa B. Nayaka
Keyword(s):  
Estrous Cycle ◽  
Ethanol Extract ◽  
Portulaca Oleracea ◽  
Albino Rats

scholarly journals The fertility assessment of normal cyclic Wistar rats following the administration of methanolic extract of Portulaca oleracea: an experimental study

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Izuchukwu Azuka Okafor ◽  
Uchenna Somtochukwu Nnamah ◽  
Jude Nnaka
Keyword(s):  
Estrous Cycle ◽  
Adult Female ◽  
Wistar Rats ◽  
Major Effect ◽  
Reproductive Hormones ◽  
Portulaca Oleracea ◽  
High Dose ◽  
Methanolic Extracts ◽  
Cycle Arrest ◽  
Female Wistar Rats

Abstract Background Purslane is a widely distributed shrub used for the treatment of different ailments. The increasing reproductive complications associated with herbal treatments have led to the need to critically evaluate the safety and/or reproductive potentials of commonly used plant extracts. This study investigated the reproductive effect of methanolic extracts of Portulaca oleracea (MEPO) in adult female Wistar rats. Results Group C showed a significant decrease both in relative ovarian weight (p = 0.000), and relative uterine weight (p = 0.037), when compared with the control. There were no significant (p ˃ 0.05) changes in the levels of follicle-stimulating hormone, luteinizing hormone, progesterone, and estradiol. When compared to the control, groups B and C showed abnormal estrous cycle and cycle arrest especially at the metestrus phase with mild congestion of a few blood vessels in the ovary and uterus. Conclusions MEPO may possess some anti-fertility effect, as it disrupts the estrous cycle of adult female Wistar rats; although it has no major effect on the reproductive hormones, uterus, and ovarian histology of adult female Wistar rats. However, high dose consumption should be taken with precaution.

scholarly journals Effect of some Nigerian traditional alcoholic beverages on the estrous cycle and histological assessment of the ovaries and uterus of albino rats

2021 ◽  
Vol 14 (1) ◽  
pp. 027-036
Author(s):  
Olusayo Moritiwon ◽  
Timothy Olugbenga Ogundeko ◽  
James Bitrus ◽  
David Oyebode ◽  
Olufunmilayo Ibiyemi Abobarin
Keyword(s):  
Corpus Luteum ◽  
Estrous Cycle ◽  
Ovarian Follicle ◽  
Alcoholic Beverages ◽  
Albino Rats ◽  
Histological Image ◽  
Follicular Cyst ◽  
Regular Consumption ◽  
Alcoholic Drinks ◽  
Corpus Luteum Cyst

Alcohol related health challenges have lingered over the years. Such associated with locally brewed alcoholic beverages have received little attention. The urgency to explore this becomes imperative. This study aimed at evaluating the effect of regular consumption of local alcoholic beverages on the estrous cycle and histological image of the ovaries and uterus thus fertility of female albino rats. Thirty screened female albino rats weighing 180-220g were divided into five groups and daily administered each with 10ml/kg of pito, burukutu, ogogoro, goskolo and 0.5ml/kg normal saline respectively for 21 days. Effect of the beverages on the estrous cycle as well as histopathological evaluation was carried out on the isolated ovaries and uterus. Results showed significant increase proestrous phase of the estrous cycle with ingestion of pito, burukutu, ogogorogo and goskolo. Also the histology of the ovary was basically without obvious pathological changes with pito, while there was alteration of histological parameters by burukutu, marked with formation of fibrosis, corpus luteum cyst resulting immature ovarian follicle, ogogoro marked with formation of fibrosis, corpus luteum cyst resulting immature ovarian follicle and goskolo marked with formation of multiple follicular cyst resulting in complete collapse of the ovarian section. The effect of traditional alcoholic beverages revealed the classical effects of alcoholic drinks by way of significant alteration in the estrous cycle of albino rats with marked alteration of the histological architecture of ovarian tissues. Pito, burukutu, ogogoro, and goskolo have a reprotoxic effect on the ovaries and uterus thus a deleterious effect on fertility of female albino rats.

scholarly journals Subacute Toxicity Effect of Psidium guajava Leaves Ethanolic Extract on Gaster in Wistar Rat

2018 ◽  
Vol 16 (1) ◽  
pp. 61
Author(s):  
Esti Dyah Utami ◽  
Hanif Nasiatul Baroroh ◽  
Nuryanti Nuryanti
Keyword(s):  
Toxic Effect ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Wistar Rat ◽  
Psidium Guajava ◽  
Albino Rats ◽  
Respiratory Disorder ◽  
Subchronic Toxicity ◽  
Toxicity Effect ◽  
Test Materials

Psidium guajava leaves have been proven be antiallergic. From the results of previous research, the ethanol extract of leaves is included in the class materials "Practically Non-Toxic." However, repeated administration may cause toxic effects on body organs, one of which was gaster. Subchronic toxicity had been carried out on 20 male Wistar rats and 20 female wistar albino rats. The test materials were administered orally, every day for 28 days. There are 3 (three) doses of test materials which were 250 mg, 500 mg, and 750 mg/kg BW. Observation of the general toxic effect was performed everyday, then organ sampling was performed on day 29 to examined gaster histology and score using Barthel-Manja methods. The result showed that administration of Psidium guajava leaves extract for 28 days caused physical and behavioral changes, caused soft and mucous feces, and also respiratory disorder. But the extract at dose 250 mg, 500 mg and 750 mg/kg BW did not caused changes on gaster histology profile and score. In conclusion, the extract did not have subchronic toxicity effect on gaster rat, but might caused general toxic effect in the higher dose.

scholarly journals Cardioprotective Potential of Ethanol Extract of Sonchus Arvensis L. Leaves on Isoproterenol-Induced Myocardial Infarction in Rat

2018 ◽  
Vol 16 (1) ◽  
pp. 20
Author(s):  
Neng Fisheri Kurniati ◽  
Elin Yulinah Sukandar ◽  
Rian Pardilah ◽  
Nova Suliska ◽  
Dhyan Kusuma Ayuningtyas
Keyword(s):  
Myocardial Infarction ◽  
Creatine Kinase ◽  
Ethanol Extract ◽  
Wistar Rat ◽  
Positive Control ◽  
Negative Control ◽  
Albino Rats ◽  
Creatine Kinase Mb ◽  
Wistar Albino Rats ◽  
Sonchus Arvensis

Sonchus arvensis L. leaves have been used traditionally to treat various disease conditions. This study is designed to evaluate cardioprotective potential of ethanol extract of S. arvensis leaves on isoproterenol-induced myocardial infarction in Wistar rat. Male Wistar albino rats were divided into three main groups: negative control (saline only), positive control (isoproterenol only), and S. arvensis extract treated groups. S. arvensis extract was administered in three doses; 50, 100, and 200 mg/kg b.w. p.o for 14 days. On day 13 and 14, isoproterenol (85 mg/kg bw) was given intraperitoneally to positive control and extract treated groups. The parameters studied were cardiac biomarker enzymes which were Creatine Kinase (CK), Creatine Kinase-MB (CK-MB), Aspartate Transaminase (AST), Alanine Transaminase (ALT) and Lactate Dehydrogenase (LDH). The results showed that S. arvensis at dose of 50 mg/kg b.w. could significantly (P<0.05) reduce the level of CK, CK-MB, AST, ALT, and LDH in myocardial infarcted rats compared to positive control. The increase of the dose of S. arvensis extract was not followed by an increase of its cardioprotective activity. In conclusion, Sonchus arvensis L. leaves extract at dose of 50 mg/kg b.w. has potential to be developed as cardioprotective drug.

Effect of Clomiphene Citrate and Letrozole on Body Weight of female Albino Rat for Consecutive 1-4 Estrous Cycles

2021 ◽  
Vol 15 (11) ◽  
pp. 2938-2941
Author(s):  
Fauzia Qureshi ◽  
Syeda Rizwana Jafri ◽  
Hafiza Sadia Ahmad ◽  
Uzma Waseem ◽  
Ursula Akif ◽  
...  
Keyword(s):  
Body Weight ◽  
Estrous Cycle ◽  
Ovulation Induction ◽  
Clomiphene Citrate ◽  
The Body ◽  
Control Group ◽  
Albino Rats ◽  
Albino Rat ◽  
Estrous Cycles ◽  
Experimental Group

Background: Ovulation induction with clomiphene citrate in women with infertility has been practiced more than 40% years but in infertile patients this treatment plan proved to be ineffective with multiple complication. Body weight plays an important role modulating reproductive development and functioning. Aim: To observe the effects on body weight of female albino rat after use of clomiphene citrate and letrozole for consecutive 1-4 estrous cycles Method: Eighty four adult female Albino rats were equally divided into three groups for this research. Body weight of each rat was measured before and after the experiment. Vaginal smear cytology of each rat was performed to study different phases of estrous cycle. Control group A was given normal saline orally , In Experimental group B rats were given letrozole (Femara) at dose 5mg/kg orally and in Experimental group C rats were given clomiphene citrate at dose 100ug/kg orally. Results: Significant weight gain is observed in rats taking clomiphene citrate as compared to letrozole Conclusion : Comiphene citrate directly affects the body weight which indirectly reduces the ovulation induction and pregnancy rate. Letrozole is good alternate for ovulation induction and for CC resistant patients. Keywords: Estrous cycle, body weight, citrate and letrozole

scholarly journals EFFECT OF ETHANOL STEM BARK EXTRACT OF BERLINIA GRANDIFLORA HUTCH AND DALZ ON MARKER LIVER ENZYMES IN RATS TREATED WITH CCl4

2018 ◽  
Vol 10 (10) ◽  
pp. 69
Author(s):  
Martin Ntiamoah Donkor ◽  
Samuel Yawson Ayikanle ◽  
Samuel Adoesomdonkor
Keyword(s):  
Olive Oil ◽  
Liver Enzymes ◽  
Day Treatment ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Bark Extract ◽  
Albino Rats ◽  
Significant Activity ◽  
Stem Bark Extract ◽  
The Impact

Objective: The objective of this work was to validate the traditional claim of the use of the stem bark of Berlinia grandiflora in treating liver problems by investigating the effect of its ethanol stem bark extract on maker liver enzymes in vivo.Methods: Groups of Wistar albino rats (6 in each group) were daily treated with CCl4 (2 ml/kg b.w., diluted with olive oil 1:1 v/v, intravenously), followed by oral administration of ethanol stem bark extract of Berlinia grandiflora (ESBG) at doses 100, 300 and 900 mg/kg b.w. respectively, 3 h after the administration of the CCl4. Serum biochemical parameters were measured 24 h at the end of the 7-day treatment period and compared to a group intoxicated with CCl4 (2 ml/kg b.w., diluted with olive oil 1:1 v/v, intravenously) alone.Results: Treatment of rats with ESBG (at doses 100, 300 and 900 mg/kg b.w. orally) reduced the impact of CCl4-induced hepatotoxicity on liver maker enzymes Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP) and total bilirubin (TB), with the 900 mg/kg b.w. showing a significant reduction (p<0.05) compared to the group intoxicated with CCl4. The extract reversed the impact of the CCl4-induced hepatotoxicity by increasing the concentration of the serum Total protein (TP) and albumin (ALB), with the 900 mg/kg b.w. dose showing a significant increment (p<0.05) compared to the group intoxicated with the CCl4.Conclusion: The investigation of the effect of Berlinia grandiflora on CCl4 induced liver damage revealed that the ethanol extract of the stem bark of the plant was able to reverse the hepatotoxicity, with the 900 mg/kg b.w. dose showing a significant activity. 

scholarly journals ANTIDIABETIC AND ANTIHYPERLIPIDAEMIC ACTIVITY OF NELUMBO NUCIFERA GAERTN ETHANOL SEED EXTRACT IN STREPTOZOTOCIN INDUCED DIABETIC RATS

2017 ◽  
Vol 9 (10) ◽  
pp. 197
Author(s):  
Alok Bhardwaj ◽  
Ketan. P. Modi
Keyword(s):  
Antioxidant Activity ◽  
Blood Glucose ◽  
Lipid Peroxides ◽  
Ethanol Extract ◽  
Diabetic Rats ◽  
Seed Extract ◽  
Serum Enzymes ◽  
Nelumbo Nucifera ◽  
Albino Rats ◽  
Intraperitoneal Dose

Objective: The main objective of the study was to investigate the effect of ethanol seed extracts of Nelumbo nucifera Gaertn (Nymphaeaceae) as antihyperglycemic, anti-hyperlipidemic and antioxidant activity in streptozotocin-induced diabetic rats.Methods: Diabetes was induced in wistar albino rats by administration of streptozotocin (single intraperitoneal dose of 60 mg/kg B. W). The ethanol extract of N. nucifera seed at a dose of 200 and 400 mg/kg body weight was administrated at a single dose per day to STZ induced diabetic rats for a period of 42 d. The different pharmacological parameters were evaluated. The effect of ethanol seed extract of N. nucifera on insulin, blood glucose, urea, creatinine, HbA1C, serum protein, albumin, globulin, serum enzymes, serum lipid profiles, lipid peroxides (LPO) and other antioxidant enzymes like catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) and reduced glutathione (GSH) were measured in the diabetic rats.Results: In the acute toxicity study ethanol seed extract of Nelumbo nucifera were non-toxic at 2000 mg/kg in rats. The ethanol extract of Nelumbo nucifera seed showed significant reduction in blood glucose (p<0.05), serum enzymes (SGPT, SGOT, ALP) (p<0.05), lipid parameters (TC, TG, VLDL, LDL) (p<0.05) except HDL and significantly increased insulin (p<0.05), HDL (p<0.05), GPx, GSH, SOD and CAT (p<0.05) at the dose of 400 mg/kg when compared with the diabetic-induced control.Conclusion: The present study suggested that the NNSE has significant (p<0.05) antihyperglycemic, antihyperlipidemic and antioxidant activity in STZ induced diabetic rats. These results clearly indicate that Nelumbo nucifera is effective against free radical-mediated diseases, thus replacing the synthetic ones.

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