Hypertension aggravates glomerular dysfunction with oxidative stress in a rat model of diabetic nephropathy

Background. Diabetic nephropathy is the most common cause of end-stage renal disease. Traditional therapy for diabetic nephropathy has focused on supportive treatment, and there is no significant effective therapy. We investigated the effect of low-energy extracorporeal shock wave therapy on a diabetic nephropathy rat model. Methods. Streptozotocin-induced diabetic nephropathy rats were treated with six sessions of low-energy extracorporeal shock wave therapy (weekly for six consecutive weeks) or left untreated. We assessed urinary creatinine and albumin, glomerular volume, renal fibrosis, podocyte number, renal inflammation, oxidative stress, and tissue repair markers (SDF-1 and VEGF) six weeks after the completion of treatment. Results. The six-week low-energy extracorporeal shock wave therapy regimen decreased urinary albumin excretion as well as reduced glomerular hypertrophy and renal fibrosis in the rat model of diabetic nephropathy. Moreover, low-energy extracorporeal shock wave therapy increased podocyte number in diabetic nephropathy rats. This was likely primarily attributed to the fact that low-energy extracorporeal shock wave therapy reduced renal inflammation and oxidative stress as well as increased tissue repair potency and cell proliferation. Conclusions. Low-energy extracorporeal shock wave therapy preserved kidney function in diabetic nephropathy. Low-energy extracorporeal shock wave therapy may serve as a novel noninvasive and effective treatment of diabetic nephropathy.

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Oleanolic acid andN-acetylcysteine ameliorate diabetic nephropathy through reduction of oxidative stress and endoplasmic reticulum stress in a type 2 diabetic rat model

Nephrology Dialysis Transplantation ◽

10.1093/ndt/gfv377 ◽

2015 ◽

Vol 31 (3) ◽

pp. 391-400 ◽

Cited By ~ 35

Author(s):

Eun Soo Lee ◽

Hong Min Kim ◽

Jeong Suk Kang ◽

Eun Young Lee ◽

Dhananjay Yadav ◽

...

Keyword(s):

Oxidative Stress ◽

Endoplasmic Reticulum ◽

Diabetic Nephropathy ◽

Endoplasmic Reticulum Stress ◽

Oleanolic Acid ◽

Rat Model ◽

Diabetic Rat ◽

Diabetic Rat Model ◽

Type 2 Diabetic

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Fenugreek Prevents the Development of STZ-Induced Diabetic Nephropathy in a Rat Model of Diabetes

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2014/259368 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 10

Author(s):

Yingli Jin ◽

Yan Shi ◽

Yinggang Zou ◽

Chunsheng Miao ◽

Bo Sun ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetic Nephropathy ◽

Growth Factor ◽

Rat Model ◽

Transforming Growth Factor ◽

Tissue Growth ◽

Diabetic Rat ◽

Dimethyl Formamide ◽

Interventional Treatment ◽

Morphological Alterations

The present study aims to examine the protective effect of fenugreek and the underlying mechanism against the development of diabetic nephropathy (DN) in streptozotocin- (STZ-) induced diabetic rats. A rat model of diabetes was successfully established by direct injection of STZ and then the rats were administered an interventional treatment of fenugreek. Parameters of renal function, including blood glucose, albuminuria, hemoglobin A1c (HbA1c), dimethyl formamide (DMF), blood urine nitrogen (BUN), serum creatinine (Scr), and kidney index (KI), were detected in the three groups (Con, DN, and DF). Oxidative stress was determined by the activity of antioxidase. Extracellular matrix (ECM) accumulation and other morphological alterations were evaluated by means of immunohistochemistry and electron microscope. Quantitive (q)PCR was employed to detect the mRNA expression of transforming growth factor-β1 (TGF-β1) and connective tissue growth factor (CTGF) and protein expression was determined with western blot analysis. DN rats in the present study demonstrated a significant renal dysfunction, ECM accumulation, pathological alteration, and oxidative stress, while the symptoms were evidently reduced by fenugreek treatment. Furthermore, the upregulation of TGF-β1 and CTGF at a transcriptional and translational level in DN rats was distinctly inhibited by fenugreek. Consequently, fenugreek prevents DN development in a STZ-induced diabetic rat model.

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Simvastatin ameliorates diabetic nephropathy by attenuating oxidative stress and apoptosis in a rat model of streptozotocin-induced type 1 diabetes

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2018.05.130 ◽

2018 ◽

Vol 105 ◽

pp. 290-298 ◽

Cited By ~ 12

Author(s):

Nawal M. Al-Rasheed ◽

Nouf M. Al-Rasheed ◽

Yieldez A. Bassiouni ◽

Iman H. Hasan ◽

Maha A. Al-Amin ◽

...

Keyword(s):

Oxidative Stress ◽

Type 1 Diabetes ◽

Diabetic Nephropathy ◽

Rat Model

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Protective Effects of Curcumin on Renal Oxidative Stress and Lipid Metabolism in a Rat Model of Type 2 Diabetic Nephropathy

Yonsei Medical Journal ◽

10.3349/ymj.2016.57.3.664 ◽

2016 ◽

Vol 57 (3) ◽

pp. 664 ◽

Cited By ~ 49

Author(s):

Bo Hwan Kim ◽

Eun Soo Lee ◽

Ran Choi ◽

Jarinyaporn Nawaboot ◽

Mi Young Lee ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetic Nephropathy ◽

Lipid Metabolism ◽

Rat Model ◽

Protective Effects ◽

Type 2 Diabetic

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Protective Effect of Swertiamarin on Myocardial Injury Through Activation of Nrf2/HO-1 Pathway in Heart Failure Model of Rats

Current Topics in Nutraceutical Research ◽

10.37290/ctnr2641-452x.18:260-265 ◽

2020 ◽

Vol 18 (3) ◽

pp. 260-265

Author(s):

Xu Lin ◽

Zheng Xiaojun ◽

Lv Heng ◽

Mo Yipeng ◽

Tong Hong

Keyword(s):

Oxidative Stress ◽

Heart Failure ◽

Ejection Fraction ◽

Protective Effect ◽

Infarct Size ◽

Rat Model ◽

Left Ventricular ◽

Related Factor ◽

Nuclear Factor ◽

Internal Dimension

The purpose of this study was to evaluate the protective effect of swertiamarin on heart failure. To this end, a rat model of heart failure was established via left coronary artery ligation. Infarct size of heart tissues was determined using triphenyl tetrazolium chloride staining. Echocardiography was performed to evaluate cardiac function by the determination of ejection fraction, left ventricular internal dimension in diastole and left ventricular internal dimension in systole. The effect of swertiamarin on oxidative stress was evaluated via enzyme-linked immunosorbent assay. The mechanism was evaluated using western blot. Administration of swertiamarin reduced the infarct size of heart tissues in rat models with heart failure. Moreover, swertiamarin treatment ameliorated the cardiac function, increased ejection fraction and fractional shortening, decreased left ventricular internal dimension in diastole and left ventricular internal dimension in systole. Swertiamarin improved oxidative stress with reduced malondialdehyde, while increased superoxide dismutase, glutathione, and GSH peroxidase. Furthermore, nuclear-factor erythroid 2-related factor 2, heme oxygenase and NAD(P)H dehydrogenase (quinone 1) were elevated by swertiamarin treatment in heart tissues of rat model with heart failure. Swertiamarin alleviated heart failure through suppression of oxidative stress response via nuclear-factor erythroid 2-related factor 2/heme oxygenase-1 pathway providing a novel therapeutic strategy for heart failure.

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Theaflavin Enriched Black Tea Extract Alleviates Diabetic Nephropathy by Suppressing Hyperglycaemia-Mediated Oxidative Stress and Inflammation in Streptozotocin-Induced Rats

The Natural Products Journal ◽

10.2174/2210315510999200607182700 ◽

2020 ◽

Vol 10 ◽

Author(s):

Dhrubajyoti Sarkar ◽

Sekhar Kumar Bose ◽

Tania Chakraborty ◽

Souvik Roy

Keyword(s):

Oxidative Stress ◽

Diabetic Nephropathy ◽

Blood Glucose ◽

Blood Urea Nitrogen ◽

Phenolic Content ◽

Black Tea ◽

Total Phenolic ◽

Urea Nitrogen ◽

Black Tea Extract ◽

Tea Extract

Background: Diabetic nephropathy (DN), a microvascular complication of diabetes has been a significant health issue globally. However, theaflavin enriched black tea extract (BTE-TF) could restrain DN. Objective: The main objective of this exploration was to elucidate the effect of BTE-TF on DN, though the underlying mechanism remains unclear and requires further investigation. Method: The tea leaves were fermented to get black tea extract. Total phenolic content and HPLC were carried out to determine the phenolic content and theaflavin in the extract. Streptozotocin induced diabetic rats were treated with 100, 200, and 400 mg/kg/day BTE-TF extract for 12 weeks. Biochemical parameters like blood glucose, creatinine, blood urea nitrogen (BUN), triglyceride and antioxidant parameters of kidney tissue were measured. Histology, immunohistochemistry and TUNEL assay were performed to observe the effect of the extract with comparison to the standard drug (Metformin 200mg/kg/day). Result: Treated animals exhibited reduced blood glucose levels, blood urea nitrogen (BUN), creatinine, and serum triglycerides. Further, BTE-TF restored the histological alterations in the kidney. Chronic hyperglycaemia resulted in a significant increase in oxidative stress and pro-inflammatory cytokines of NF-kβ pathway. BTE-TF attenuated oxidative stress (p<0.01), inflammation (p<0.05) and apoptosis (p<0.05). Conclusion: This study suggests that BTE-TF exerts a protective role against diabetes-induced renal injury by ameliorating oxidative stress, inflammation, and apoptosis.

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