Detoxification of aflatoxin B 1 by lactic acid bacteria and hydrated sodium calcium aluminosilicate in broiler chickens

A comparative test of the viability of the fodder complex «Alhalal», containing in its composition of microalgae and lactic acid bacteria on chickens-broilers. A positive result was obtained, confirmed by a number of Exo-endogenous indicators.

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Effect of Lactic Acid Bacteria Strains on the Growth and Aflatoxin Production Potential of Aspergillus parasiticus, and Their Ability to Bind Aflatoxin B1, Ochratoxin A, and Zearalenone in vitro

Frontiers in Microbiology ◽

10.3389/fmicb.2021.655386 ◽

2021 ◽

Vol 12 ◽

Author(s):

Cleide Oliveira de Almeida Møller ◽

Luisa Freire ◽

Roice Eliana Rosim ◽

Larissa Pereira Margalho ◽

Celso Fasura Balthazar ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Ochratoxin A ◽

Aspergillus Parasiticus ◽

Potassium Phosphate ◽

Aflatoxin Production ◽

Inhibitory Effect ◽

Binding Studies ◽

Aflatoxin B

The increased consumption of plant-based foods has intensified the concern related to mycotoxin intoxication. This study aimed to investigate the effect of selected lactic acid bacteria (LAB) strains on the growth of Aspergillus parasiticus NRRL 2999 and its production of aflatoxin (AF). The ability of the heat-killed (100°C for 1 h) LAB strains to bind aflatoxin M1 (AFM1) in milk and aflatoxin B1 (AFB1), ochratoxin A (OTA), and zearalenone (ZEN) in potassium phosphate buffer (PPB) was also evaluated in vitro. Ten LAB strains were tested individually, by inoculating them simultaneously with the fungus or after incubation of the fungus for 24 or 48 h at 25°C. Double layer yeast extract sucrose (YES) agar, de Man Rogosa and Sharpe (MRS) agar, and YES broth were incubated for 7 days at 25°C to follow the development of the fungus. Levilactobacillus spp. 3QB398 and Levilactobacillus brevis 2QB422 strains were able to delay the growth of A. parasiticus in YES broth, even when these strains were inoculated 24 h after the fungus. The inhibitory effect of these LAB strains was confirmed by the reduction of fungus colony size, suggesting dominance of LAB by competition (a Lotka-Voltera effect). The production of AFB1 by A. parasiticus was inhibited when the fungus was inoculated simultaneously with Lactiplantibacillus plantarum 3QB361 or L. plantarum 3QB350. No AFB1 was found when Levilactobacillus spp. 2QB383 was present, even when the LAB was inoculated 48 h after the fungus. In binding studies, seven inactivated LAB strains were able to promote a reduction of at least 50% the level of AFB1, OTA, and ZEN. This reduction varied depending on the pH of the PPB. In milk, however, only two inactivated LAB strains were able to reduce AFM1, with a reduction of 33 and 45% for Levilactobacillus spp. 3QB398 (Levilactobacillus spp.) and L. brevis 2QB422, respectively. Nevertheless, these results clearly indicate the potential of using LAB for mycotoxin reduction.

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Pengaruh Probiotik Bakteri Asam Laktat dalam Air Minum Terhadap Konversi Pakan Ayam Broiler

Journal of Basic Medical Veterinary ◽

10.20473/jbmv.v9i2.28587 ◽

2021 ◽

Vol 9 (2) ◽

pp. 86

Author(s):

Nanda Kholifatul Azizah ◽

Sarmanu Sarmanu ◽

Budi Utomo ◽

Emy Koestanti Sabdoningrum ◽

Widya Paramitha Lokapirnasari ◽

...

Keyword(s):

Drinking Water ◽

Lactic Acid ◽

Weight Gain ◽

Lactic Acid Bacteria ◽

Broiler Chickens ◽

Body Weight Gain ◽

Feed Consumption ◽

Feed Conversion ◽

Conversion Rates ◽

Probiotic Lactic Acid Bacteria

This study aimed to determine the effect of probiotics of lactic acid bacteria in drinking water on feed consumption, weight gain and feed conversion ratio of broiler chickens. This research is an experimental research. The experimental animals used in this study were 28 male broiler chickens strain Cobb 700 aged 20 days. P0 was not given the addition of probiotic lactic acid bacteria, P1 was given the addition of probiotic lactic acid bacteria Lactobacillus acidophilus in 1ml/liter drinking water, P2 was given the addition of probiotic lactic acid bacteria Lactobacillus casei in drinking water 1ml/liter, P3 was given the addition of probiotic lactic acid bacteria Lactobacillus fermentum in drinking water 1ml/liter, P4 was given the addition of probiotic lactic acid bacteria Lactobacillus plantarum in drinking water 1ml/liter, P5 was given the addition of probiotic lactic acid bacteria Pediococcus in drinking water 1ml/liter, P6 was given the addition of probiotic mix lactic acid bacteria in drinking water 1ml /liter. Collecting data for the last 1 week by weighing the remaining consumption of feed given and weight gain of broiler chickens. The data obtained were analyzed using the Analysis of Variant (ANOVA) statistical method and continued with Duncan. Based on the results of the calculation of the amount of feed consumption and weight gain in broiler chickens, it showed that the administration of probiotic lactic acid bacteria 1ml/liter of water in broiler chickens could increase body weight gain, reduce feed conversion rates but did not reduce broiler feed consumption (p<0.05).

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Effect of a Synbiotic Mix on Intestinal Structural Changes, and Salmonella Typhimurium and Clostridium Perfringens Colonization in Broiler Chickens

Animals ◽

10.3390/ani9100777 ◽

2019 ◽

Vol 9 (10) ◽

pp. 777 ◽

Cited By ~ 4

Author(s):

Zuamí Villagrán-de la Mora ◽

Karla Nuño ◽

Olga Vázquez-Paulino ◽

Hugo Avalos ◽

Javier Castro-Rosas ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Salmonella Typhimurium ◽

Broiler Chickens ◽

Structural Changes ◽

Duodenal Mucosa ◽

Control Group ◽

Histopathological Analysis ◽

Gastrointestinal Infections ◽

Isolation Frequency

Synbiotics can prevent gastrointestinal infections in broilers. This work studies the effect of a Synbiotic on broilers. One-day-old male broilers were divided into groups: Control; Synbiotic; Synbiotic + S. Typhimurium; Synbiotic + C. perfringens; Synbiotic + S. Typhimurium + C. perfringens; S. Typhimurium; C. perfringens; and S. Typhimurium + C. perfringens. Histopathological analysis revealed that the Synbiotic promoted longer villi, less deep crypts, and better villi-crypt ratio. Broilers treated with the Synbiotic, infected with pathogens or not, had healthier mucosa. In groups infected with pathogens, the frequency and intensity of histopathologic lesions were lessened often in groups treated with the Synbiotic. The Synbiotic group had higher lactic acid bacteria counts than the Control group on day 39, and the isolation frequency of S. Typhimurium was lower (p < 0.05) in the Synbiotic-treated groups. On day 18, mucosa, villi, villi-crypt ratio, crypt, and feed intake were influenced by Enterobacteriaceae. However, on day 39 (end of the trial), those parameters were influenced by lactic acid bacteria. The Synbiotic influenced morphological modifications in the duodenal mucosa, which in turn gave the broilers the ability to resist infections caused by S. Typhimurium and C. perfringens, by inhibiting their growth and decreasing the intensity and frequency of histopathological injuries.

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Suppression on the Aflatoxin-B Production and the Growth of Aspergillus flavus by Lactic Acid Bacteria (Lactobacillus delbrueckii, Lactobacillus fermentum and Lactobacillus plantarum)

Biotechnology(Faisalabad) ◽

10.3923/biotech.2007.257.262 ◽

2007 ◽

Vol 6 (2) ◽

pp. 257-262 ◽

Cited By ~ 8

Author(s):

I. Nyoman Pugeg Aryantha ◽

Arina Tri Lunggani

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Lactobacillus Plantarum ◽

Aspergillus Flavus ◽

Lactobacillus Delbrueckii ◽

Lactobacillus Fermentum ◽

Aflatoxin B

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Effects of Feeding of Two Potentially Probiotic Preparations from Lactic Acid Bacteria on the Performance and Faecal Microflora of Broiler Chickens

The Scientific World JOURNAL ◽

10.1100/2012/562635 ◽

2012 ◽

Vol 2012 ◽

pp. 1-9 ◽

Cited By ~ 15

Author(s):

Paula Fajardo ◽

Lorenzo Pastrana ◽

Jesús Méndez ◽

Isabel Rodríguez ◽

Clara Fuciños ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Lactobacillus Casei ◽

Broiler Chickens ◽

Body Weight Gain ◽

Live Weight ◽

Feed Consumption ◽

Weaned Piglets ◽

Intestinal Weight ◽

Faecal Microflora

The aim of this study was to evaluate the potential of two probiotic preparations, containing live lactic acid bacteria (Lactococcus lactisCECT 539 andLactobacillus caseiCECT 4043) and their products of fermentation (organic acids and bacteriocins), as a replacement for antibiotics in stimulating health and growth of broiler chickens. The effects of the supplementation of both preparations (with proven probiotic effect in weaned piglets) and an antibiotic (avilamycin) on body weight gain (BWG), feed intake (FI), feed consumption efficiency (FCE), relative intestinal weight, and intestinal microbiota counts were studied in 1-day posthatch chickens. The experiments were conducted with medium-growth Sasso X44 chickens housed in cages and with nutritional stressed Ross 308 broiler distributed in pens. Consumption of the different diets did not affect significantly the final coliform counts in Sasso X44 chickens. However, counts of lactic acid bacteria and mesophilic microorganisms were higher in the animals receiving the two probiotic preparations (P<0.05). In the second experiment, although no differences in BWG were observed between treatments, Ross 308 broilers receiving the probioticLactobacilluspreparation exhibited the lowest FCE values and were considered the most efficient at converting feed into live weight.

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Effect of lactic acid bacteria probiotic culture for the treatment of Salmonella enterica serovar Heidelberg in neonatal broiler chickens and turkey poults

Poultry Science ◽

10.3382/ps.2010-01220 ◽

2011 ◽

Vol 90 (3) ◽

pp. 561-565 ◽

Cited By ~ 44

Author(s):

A. Menconi ◽

A.D. Wolfenden ◽

S. Shivaramaiah ◽

J.C. Terraes ◽

T. Urbano ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Salmonella Enterica ◽

Broiler Chickens ◽

Turkey Poults

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Evaluation of a selected lactic acid bacteria-based probiotic on Salmonella enterica serovar Enteritidis colonization and intestinal permeability in broiler chickens

Avian Pathology ◽

10.1080/03079457.2016.1222808 ◽

2016 ◽

Vol 46 (1) ◽

pp. 90-94 ◽

Cited By ~ 18

Author(s):

Omar F. Prado-Rebolledo ◽

Jaime de Jesus Delgado-Machuca ◽

Rafael J. Macedo-Barragan ◽

Luis J. Garcia-Márquez ◽

Jesus E. Morales-Barrera ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Intestinal Permeability ◽

Salmonella Enterica ◽

Broiler Chickens ◽

Salmonella Enterica Serovar Enteritidis

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Effect of Fermented Lime Waste Flour on the pH of the Small Intestine and Microbes of Broiler Chicken Ileum

Jurnal Sain Peternakan Indonesia ◽

10.31186/jspi.id.16.4.328-333 ◽

2021 ◽

Vol 16 (4) ◽

pp. 328-333

Author(s):

H. D. Shihah ◽

D. Sunarti ◽

S. Sumarsih

Keyword(s):

Small Intestine ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Digestive Tract ◽

Broiler Chickens ◽

Pathogenic Bacteria ◽

Ph Value ◽

Bacteria Growth ◽

Randomized Design ◽

Range Test

The balancing of digestive tract microbe can improve the digestive health of broiler chickens. Fermented lime waste flour (FLWF) contains citric acid, which can decrease digestive tract pH value to suppress pathogenic bacteria development and improve lactic acid bacteria growth in the small intestine of the broiler. The purpose of the study was to evaluate the effect of using FLWF on pH value and small intestine microbial of broiler chickens. This study used 200 female broiler chickens. The research used a completely randomized design with four treatment levels of FLWF by 0%, 1%, 2%, and 3% in every ration, with each treatment replicated five times. The parameters observed are the pH value of the small intestine, lactic acid bacteria ileum, and Coliform ileum. Data were calculated using the analysis of variance and difference test with Duncan's Multiple Range Test using the SPSS 19.0 program. The result indicates that using FLWF decreased (p<0.05) Coliform in the ileum, while pH value of small intestine and ileum lactic acid bacteria among treatments were not influenced (p>0.05). It concludes that adding FLWF at a 1% level could decrease ileum Coliform. Still, it could not decrease the pH value of the small intestine and increase the total lactic bacteria ileum.

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