scholarly journals Effect of Fermented Lime Waste Flour on the pH of the Small Intestine and Microbes of Broiler Chicken Ileum

2021 ◽  
Vol 16 (4) ◽  
pp. 328-333
Author(s):  
H. D. Shihah ◽  
D. Sunarti ◽  
S. Sumarsih
Keyword(s):  
Small Intestine ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Digestive Tract ◽  
Broiler Chickens ◽  
Pathogenic Bacteria ◽  
Ph Value ◽  
Bacteria Growth ◽  
Randomized Design ◽  
Range Test

The balancing of digestive tract microbe can improve the digestive health of broiler chickens. Fermented lime waste flour (FLWF) contains citric acid, which can decrease digestive tract pH value to suppress pathogenic bacteria development and improve lactic acid bacteria growth in the small intestine of the broiler. The purpose of the study was to evaluate the effect of using FLWF on pH value and small intestine microbial of broiler chickens. This study used 200 female broiler chickens. The research used a completely randomized design with four treatment levels of FLWF by 0%, 1%, 2%, and 3% in every ration, with each treatment replicated five times. The parameters observed are the pH value of the small intestine, lactic acid bacteria ileum, and Coliform ileum. Data were calculated using the analysis of variance and difference test with Duncan's Multiple Range Test using the SPSS 19.0 program. The result indicates that using FLWF decreased (p<0.05) Coliform in the ileum, while pH value of small intestine and ileum lactic acid bacteria among treatments were not influenced (p>0.05). It concludes that adding FLWF at a 1% level could decrease ileum Coliform. Still, it could not decrease the pH value of the small intestine and increase the total lactic bacteria ileum. 

scholarly journals PENGARUH KONSENTRASI STARTER BAKTERI Lactobacillus plantarum TERHADAP BEBERAPA KOMPONEN MUTU TEPUNG PORANG (Amorphophallus oncophyllus)

Pro Food ◽  
2021 ◽  
Vol 6 (2) ◽  
pp. 673
Author(s):  
Ade Irma Juliana ◽  
Moegiratul Amaro ◽  
Nazaruddin Nazaruddin
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Protein Content ◽  
Lactobacillus Plantarum ◽  
Analysis Of Variance ◽  
Ph Value ◽  
Significance Level ◽  
Randomized Design ◽  
Significant Difference ◽  
Polynomial Orthogonal

ABSTRACT               This study aims to determine the effect of Lactobacillus plantarum bacterial starter concentration on some quality of the porang flour. This study used experimental design one-factor Completely Randomized Design (CRD) consisted of BAL concentration (KB) of Lactobacillus plantarum with 6 treatments which is 0%, 5%, 10%, 15%, 20% and 25%. Each of treatment were repeated three times to obtain 18 unit samples. Data from observations were analyzed using analysis of variance (Analysis of Variance) at 5% significance level using Co-stat software. If there are significant differences, a further Polynomial Orthogonal and Honestly Significant Difference (BNJ) test is performed at a level of 5%. The parameters observed included pH value, protein content, water content, yield, total lactic acid bacteria, organoleptic parameters of color and aroma (hedonic and scoring). The results showed that the concentration of Lactobacillus plantarum bacterial 20% was the best treatment in producing porang flour, pH value 5.72, protein content 6.49%, yield 9.33%, total lactic acid bacteria 6.66 log CFU / g and color rather brown and slightly acidic aroma and somewhat preferred by panelists. Keywords: Porang flour, starter concentration, Lactobacillus plantarum   ABSTRAK             Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi starter bakteri Lactobacillus plantarum terhadap beberapa komponen mutu tepung porang. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor yakni konsentrasi BAL (KB) jenis Lactobacillus plantarum dengan 6 perlakuan yaitu 0%, 5%, 10%, 15%, 20% dan 25%. Masing-masing perlakuan diulang sebanyak 3 kali sehingga diperoleh 18 unit percobaan. Data hasil pengamatan dianalisis menggunakan analisis keragaman (Analysis of Variance) pada taraf nyata 5% menggunakan software Co-stat. Apabila terdaapat beda nyata, dilakukan uji lanjut Polynomial Orthogonal dan Beda Nyata Jujur (BNJ) pada taraf 5%. Adapun parameter yang diamati meliputi nilai pH, kadar protein, kadar air, rendemen, total bakteri asam laktat, parameter organoleptik warna dan aroma (hedonik dan scoring). Hasil penelitian menunjukkan bahwa konsentrasi strater bakteri Lactobacillus plantarum 20% merupakan perlakuan terbaik dalam menghasilkan tepung porang nilai pH 5,72, kadar protein 6,49%, rendemen 9,33%, total bakteri asam laktat 6,66 log CFU/g serta warna agak coklat dan aroma agak asam serta agak disukai panelis.    Kata Kunci: Tepung porang, konsentrasi starter, Lactobacillus plantarum

scholarly journals The effects of low-temperature storage on the viability of Lactobacillus casei and the stability of antibacterial activity in green grass jelly synbiotic drinks

2020 ◽  
Vol 21 (8) ◽  
Author(s):  
SAMSUL RIZAL ◽  
Suharyono Suharyono ◽  
Fibra Nuariny ◽  
Julfi Restu Amelia
Keyword(s):  
Antibacterial Activity ◽  
Lactic Acid ◽  
Low Temperature ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Casei ◽  
Pathogenic Bacteria ◽  
Ph Value ◽  
Low Temperature Storage ◽  
The Stability ◽  
Temperature Storage

Abstract. Rizal S, Suharyono, Nurainy F, Amela JR. 2020. The effects of low-temperature storage on the viability of Lactobacillus casei and the stability of antibacterial activity in green grass jelly synbiotic drinks. Biodiversitas 21: 3826-3831. Synbiotic drinks from green grass jelly have shown antibacterial activity against pathogenic bacteria. These are usually stored at low temperatures to maintain their characteristics. The aim of this study was to determine the effect of storage at low temperature of 10°C on the viability of lactic acid bacteria (Lactobacillus casei) and the stability of the antibacterial activity in synbiotic drinks made of green grass jelly. Antibacterial activity of green grass jelly synbiotic drink was conducted against pathogenic bacteria (Staphylococcus aureus, Salmonella sp., Bacillus cereus, and Escherichia coli). The products were stored for 28 days at 10°C temperature. Observations on the antibacterial activity, pH value, total acid, and total lactic acid bacteria were carried out every 7 days. Antibacterial activity was evaluated using agar well diffusion method. The results showed that storage at low temperature (10 ± 2°C) for 28 days decreased the antibacterial activity and pH value but sharply increased total lactic acid bacteria (at 0 to 7 days of storage) in green grass jelly synbiotic drinks. Salmonella sp. showed the highest inhibition caused by the antibacterial agents in green grass jelly synbiotic drinks while the lowest inhibition was found on Staphylococcus aureus. During storage at low temperature, green grass jelly synbiotic drinks had a total of lactic acid bacteria that ranged from 9.51 to 10.10 (Log CFU/mL) or equal to 3.24x109-1.26x1010 CFU/mL; a total of lactic acid that ranged from 0.48% to 0.87%; and pH values that ranged from 3.78 to 4.08.

scholarly journals Bacteriocin Activity of Lactic Acid Bacteria Isolated from Rumen Fluid of Thin Tail Sheep

2019 ◽  
Vol 43 (3) ◽  
Author(s):  
Okti Widayati ◽  
Zaenal Bachruddin ◽  
Chusnul Hanim ◽  
Lies Mira Yusiati ◽  
Nafiatul Umami
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Ph Value ◽  
Heating Temperature ◽  
Rumen Fluid ◽  
Bacteriocin Activity ◽  
Significant Difference ◽  
Sheep Rumen ◽  
The Stability ◽  
Range Test

The objective of this study was to determine the activity and the stability of bacteriocin from lactic acid bacteria (BAL) isolated from rumen fluid of thin-tail sheep under the temperature (80, 100, and 121°C), pH (3, 7, and 10), and the length of storage (for 2 weeks under the temperature -8, 11, and 29°C). Lactic acid bacteria obtained by isolation, selection, and identification of thin-tailed sheep rumen fluid were used for bacteriocin production. The crude bacteriocin was partially purified using 70% ammonium sulfate, then was dialysis for 12 hours. The obtained bacteriocin then tested its inhibitory activity against E.coli (representing Gram-negative) and S. aureus (representing Gram-positive) under temperature (80, 100, and 121°C), pH (3, 7, and 10), and the length of storage (for 2 weeks under the temperature -8, 11, and 29°C). The data of bacteriocin activity based on pH, temperature, and the length of storage were analyzed with factorial, then when there was a significant difference of variable because treatment was continued with Duncan's Multiple Range Test (DMRT) test. The results showed that the bacteriocin activity of the three types of BAL against S.aureus is greater than E.coli. The highest activity was shown in pH 3, while the lowest activity was shown at pH 10 (P<0.01). The highest activity was shown at a heating temperature of 100°C, while the lowest activity was shown at a heating temperature of 80°C (P<0.01). The activity of bacteriocin produced by BAL 0 A, BAL 1 A, and BAL 4 C tended to be stable to the heating temperature of 80, 100, and 121°C but decreased with increasing pH value (pH 3, 7, and 10). The best of bacteriocin activity was found at pH 3 (acid), heating at 100°C, and stored at -8°C for 14 days.

Isolation of lactic acid bacteria from chickens that demonstrate probiotic properties of autoaggregation and coaggregation with Salmonella enteritidis

2007 ◽  
Vol 2007 ◽  
pp. 104-104
Author(s):  
S Savvidou ◽  
J. Beal ◽  
P. Brooks
Keyword(s):  
Small Intestine ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Salmonella Enteritidis ◽  
Pathogenic Bacteria ◽  
Probiotic Properties ◽  
Pathogenic Microorganisms ◽  
Poultry Products ◽  
Salmonella Infections ◽  
Live Bird

The association of salmonella infections with the consumption of poultry products and the fact that in the live bird the Salmonella carriage is mainly asyptomatic have led to a demand for finding ways of preventing infection of commercially reared poultry and product contamination (Revolledo et al., 2006). One approach is the use of probiotics. The probiotic properties of lactic acid bacteria have been widely studied. Their capacity for adhesion to mucus, ability to autoaggregate and potential for coaggregation with pathogenic bacteria are potential mechanisms for providing a competitive advantage in the intestinal microbiota (Ghadban et al, 2002) and forming a barrier that prevents colonization of pathogenic microorganisms (Kos et al, 2003). In this study, a total of 53 lactic acid bacteria (LAB) were isolated from the contents of the crop, caecum and small intestine, and from the mucosa of the crop, jejunum and ileum of three organically farmed chickens, were examined for autoaggregation and coaggregation with Salmonella enteritidis.

scholarly journals SIFAT KIMIA DAN MIKROBIOLOGI BAKASANG IKAN OCI (Rastrelliger sp) DENGAN LAMA FERMENTASI YANG BERBEDA

Pro Food ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 397
Author(s):  
Anto Anto ◽  
Deyvie Xyzquolyna ◽  
Viene Valentine H. Ali
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Protein Content ◽  
Water Content ◽  
Design Pattern ◽  
Research Method ◽  
Ph Value ◽  
Fermentation Time ◽  
Fish Market ◽  
Randomized Design

ABSTRACT This study aimed to determine the effect of oci fermentation time on total microbes, total lactic acid bacteria, water content, protein content, and pH value. The oci fish used are fresh oci fish from the fish market in Gorontalo, then fermented for 0, 3, 10 and 15 days. The research method used the experimental method through a complete randomized design pattern using variance analysis followed by the Duncan test. For 15 days fermented, the pH value, water content and microbial total decreased, but the protein content and the number of lactic acid bacteria increased. The bakasang of oci at the end of fermentation has a total lactic acid bacteria of 2.51x107 CFU/g, the water content of 65.56%, the protein content of 5.98%, and pH value of 6.0. Keywords: oci fish, fermentation, bakasang, lactic acid bacteria   ABSTRAK Tujuan penelitian ini adalah untuk menganalisa sifat kimia dan mikrobiologi bakasang ikan oci dengan lama fermentasi yang berbeda. Ikan oci yang digunakan adalah ikan oci segar dari pasar ikan di Gorontalo, lalu difermentasi selama 3, 10 dan 15 hari. Parameter pengamatannya meliputi sifat kimia yang terdiri dari kadar air, kadar protein, nilai pH. Sedangkan sifat mikrobiologi yang diamati yaitu total mikroba dan total bakteri asam laktat. Metode penelitian ini menggunakan rancangan acak lengkap dan analisis sidik ragam dilanjutkan dengan uji Duncan. Selama 15 hari difermentasi, nilai pH, kadar air dan total mikroba menurun, namun kadar protein dan jumlah bakteri asam laktat mengalami peningkatan. Bakasang ikan oci pada akhir fermentasi mempunyai total bakteri asam laktat 2,51x107 cfu/g, kadar air 65,56%, kadar protein 5,98%, dan nilai pH 6,0. Kata kunci: ikan oci, fermentasi, bakasang, bakteri asam laktat

scholarly journals Effects of Addition Chili Ground to The Characteristics of Bekasam from Cork Fish (Channa striata) During Fermentation

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Normayanti Normayanti ◽  
Dewi Kartika Sari ◽  
Rita Khairina
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Ph Value ◽  
Sensory Properties ◽  
Total Acid ◽  
Acid Ph ◽  
Randomized Design ◽  
Fish Product ◽  
Channa Striata ◽  
Positive Effect

Bekasam is a fermented fish product made from fish, salt, and roasted rice or rice that is mixed and fermented for 7 days. This study aims to determine the effect of the addition of ground chili at different concentrations on total acid, pH value, total lactic acid bacteria, and sensory properties of cork fish bekasam. The research design used was completely randomized design, 3 treatments and 3 replications. The treatments given were IBG without the addition of ground chili, IBGC1 adding 10% ground chili and IBGC2 adding 20% ground chili. Data were collected for total acid, pH, and total lactic acid bacteria fermentation days 1, 3, 5, and 7. The sensory properties were observed on days 1 and 7. The results showed that the addition of ground chili can reduce pH during 7 days fermentation, but the increase in total acid was not significant. The addition of 10% ground chili has a positive effect on the growth of lactic acid bacteria. The addition of 20% ground chili showed an inhibition of the growth of lactic acid bacteria during fermentation. Sensory test results stated that panelists preferred bekasam with the addition of 10% ground chili compared to control brakes and the addition of 20% ground chili.

scholarly journals KUALITAS FISIKOKIMIA DAN MIKROBIA YOGHURT SINBIOTIK YANG DIBERI PATI TERMODIFIKASI UMBI UWI UNGU (Dioscorea alata) DENGAN LEVEL BERBEDA

ZOOTEC ◽  
2019 ◽  
Vol 40 (1) ◽  
pp. 124
Author(s):  
Agnes C. Korengkeng ◽  
A. Yelnetty ◽  
Rahmawati Hadju ◽  
M. Tamasoleng
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Ph Value ◽  
Modified Starch ◽  
Dioscorea Alata ◽  
Microbial Quality ◽  
Randomized Design ◽  
Completely Randomized Design

THE PHYSICOCHEMICAL AND MICROBIAL QUALITY OF SINBIOTIC YOGHURT GIVEN DIFFERENT LEVEL OF MODIFIED STARCH OF PURPLE UWI BULB (Dioscorea alata). An experiment was done in order to determine the level of modified starch of purple uwi bulb (Dioscorea alata) which best for syneresis, pH and total lactic acid bacteria of synbiotic yoghurt.  Completely Randomized Design (CRD) was used in this experiment with 6 treatments as follows P0 = yoghurt without modified starch purple uwi bulb, P1 = yoghurt + 0,1% modified starch purple uwi bulb, P2 = yoghurt + 0,2% modified starch purple uwi bulb, P3 = yoghurt + 0,3% modified starch purple uwi bulb, P4 = yoghurt + 0,4%  modified starch purple uwi bulb dan P5 = yoghurt + 0,5 modified starch purple uwi bulb; and three repetitions   each treatment. Measurements taken were syneresis, pH value, total of acid lactate bacteria. Data obtained were analyzed using ANOVA and Tuckey’s test was used to inspect treatment differences among group means.  It found in this research that application of modified starch of purple uwi bulb in symbiotic yoghurt gave a significant effect (P<0,01) on pH value and total lactic acid bacteria but not on syneresis. However, the syneresis values tendency decreased as the level of modified starch of purple uwi bulb increased.  The pH of symbiotic yoghurts given modified starch were ranged between 5.0 – 5.4 and the lowest pH was found in yoghurt with 0.3% modified starch added, meanwhile the total lactic acid bacteria of symbiotic yoghurts given modified starch were ranged between log 10-78.65 – log10-7 8.87 and the highest total lactic acid bacteria was found in yoghurt with 0.2% modified starch added.  It concluded in this research that the best quality of symbiotic yoghurt was in yoghurt with addition of modified starch of purple uwi bulb 0.3%.  Keywords: Sinbiotic Yoghurt, Modified Starch of Ubi Purple Tuber, Sineresis, pH value, BAL Total

scholarly journals Analisa Keasaman dan Total Bakteri Asam Laktat Yogurt Akibat Bahan Baku dan Persentase Lactobacillus casei yang Berbeda

2013 ◽  
Vol 13 (2) ◽  
pp. 31-35
Author(s):  
Yusdar Zakaria ◽  
Yurliasni Yurliasni ◽  
Mira Delima ◽  
Ely Diana
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Casei ◽  
Ph Value ◽  
Acid Value ◽  
Fresh Milk ◽  
Randomized Design ◽  
Significant Difference ◽  
Two Factors ◽  
Completely Randomized Design

Analyze of acidity and amount of lactic acid bacteria of yogurt made from different milk types and lactobacillus casei percentageABSTRACT. The objective of this experiment is to determine the effect of milk types and percentages of Lactobacillus casei as a starter to the amount of Lactic Acid Bacteria in yogurt and its pH value and Lactic Acid value.  Factorial Completely Randomized Design with two factors and three replications was applied.  The A factor was the milk types that were a1 = Powder Milk, a2 = Fresh Milk and a3 = UHT milk.  The B factor was the percentages of Lactobacillus casei  (b1 = 5% and b2 = 10%).  Parameters observed were the level of lactic acid, the pH value of yogurt and the amount of lactic acid bacteria.  The result showed  significantly difference (P0.01) from the use of milk types and Lactobacillus casei percentages on the lactic acid level, and there are no interaction between factors.  There was also significant different (P0.01) showed from pH value and an interaction between both factors was presented.  In addition there was significant difference (P0.05) showed by the amount of lactic acid bacteria, and an interaction between both factors was also existed.  The research concluded that the higher the percentage of starter, the higher the level of lactic acid and the amount of lactic acid bacteria of yogurt that made from all milk types.  On the contrary, the higher the percentage of starter, the lower the pH value of yogurt.

scholarly journals Pengaruh Variasi Konsentrasi Sukrosa Terhadap Total Bakteri Asam Laktat, pH, Kadar Alkohol dan Hedonik Water Kefir Belimbing Manis (Averrhoa carambola)

2021 ◽  
Vol 21 (1) ◽  
pp. 54
Author(s):  
Heni Rizqiati ◽  
Dhea Luthfia Ramadhanti ◽  
Mohammad Ihsan Yahya Prayoga
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Ph Value ◽  
Sucrose Concentration ◽  
Duncan Multiple Range Test ◽  
Alcohol Content ◽  
Averrhoa Carambola ◽  
Parametric Data ◽  
Range Test ◽  
Water Kefir

Penelitian ini bertujuan untuk mengetahui pengaruh penambahan sukrosa dengan beberapa konsentrasi yang berbeda terhadap total bakteri asam laktat, nilai pH, kadar alkohol, dan uji hedonik water kefir belimbing manis. Desain percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 4 kali ulangan, dengan perlakuan penambahan beberapa konsentrasi sukrosa (0%, 3%, 6%, 9%, dan 12%). Belimbing yang digunakan adalah jenis belimbing manis dengan menggunakan grain water kefir sebanyak 5% dan difermentasi selama 24 jam. Kualitas water kefir belimbing yang dianalisis yakni total bakteri asam laktat, nilai pH, kadar alkohol dan kesukaan produk. Data parametrik yang diperoleh kemudian dianalisis dengan aplikasi SPSS 22.0 menggunakan Analysis of Variance (ANOVA) yang dilanjutkan dengan Duncan Multiple Range Test (DMRT), sedangkan data non parametrik dianalisis dengan uji Mann-Whitney yang dilanjutkan dengan uji Kruskall-Wallis. Hasil penelitian menunjukkan bahwa penambahan sukrosa dengan beberapa konsentrasi yang berbeda memberikan pengaruh nyata terhadap total bakteri asam laktat, kadar alkohol, nilai pH dan uji hedonik. Semakin tinggi konsentrasi sukrosa yang ditambahkan maka semakin tinggi total bakteri asam laktat, alkohol, dan hedonik serta menurunkan nilai pH pada produk, perlakuan terbaik terdapat pada perlakuan T3 dengan penambahan sukrosa sebanyak 9% yang menghasilkan total bakteri asam laktat sebesar 8,746, nilai pH sebesar 4,32, kadar alkohol sebesar 1,05%, dan memiliki skor kesukaan sebesar 3,76.Kata kunci : Alkohol; belimbing; pH; water kefir The Effect of Variation Sucrose Concentration on Total Lactic Acid Bacteria, pH, Alcohol Content and Hedonic in Water Kefir from Starfruit (Averrhoa carambola) ABSTRACTThis research was to determine the effect of sucrose addition on total lactic acid bacteria, pH value, alcohol content, and hedonic test in water kefir from starfruit. The experimental design used was Completely Randomized Design (CRD) with 5 treatments and 4 replications, with treatment addition some concentration sucrose (0%, 3%, 6%, 9%, and 12%). Starfruit used is a type of sweet starfruit with a kefir water grain of 5% and fermented for 24 hours. Starfruit water kefir quality analyzed are total lactic acid bacteria, pH value, alcohol content and hedonic properties of the product. The resulted parametric data is then analyzed using Analysis of Variance (ANOVA) followed by Duncan Multiple Range Test (DMRT), while the non parametric data were analyzed using the Mann-whitney test followed by the Kruskall-wallis test.  The results showed that the addition of sucrose with several different concentrations had a significant effect on  total lactic acid bacteria, alcohol content, pH value and hedonic test. The higher sucrose concentration added the higher the resulted level on the total lactic acid bacteria, alcoholc and hedonic level also lower the pH value of the product, the best treatment is T3 with the addition of 9% sucrose which produces a total of 8,746 lactic acid bacteria, pH value of 4,32, alcohol content of 1,05%, and has a favorite score of 3,76.Keywords : Alcohol; starfruit; pH; water kefir

scholarly journals Feeding Of Calcium and Protein Macroparticle Or Microparticle With Bitter Mustard Root On Intestinal Bacteria Population And Nutrient Intake In Quail

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Lilik Krismiyanto ◽  
Nyoman Sutama ◽  
Vitus Dwi Yunianto ◽  
Fajar Wahyono ◽  
Citra Ardelia ◽  
...  
Keyword(s):  
Small Intestine ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Calcium Intake ◽  
Nutrient Intake ◽  
Intestinal Bacteria ◽  
Average Weight ◽  
Feed Ingredients ◽  
Randomized Design ◽  
Protein Feed

This study aims to feeding of protein source feed ingredients in macroparticle or microparticles with the addition of bitter mustard root to the small intestinal bacteria population and nutrient intake in quails. The experimental livestock used by the layer phase quail were 200 birds with an average weight of 149 ± 10.88 g, protein feed ingredients in the form of soybean meal and fish meal, calcium comes from eggshells and mustard root powder as a prebiotic. The research design was a completely randomized design with 4 treatment and 5 replaice (each 10 bird). The treatment were P1= Feed with protein and calcium macroparticles; P2= Feed with protein and calcium microparticles; P3= Feed with protein and calcium macroparticles + bitter mustard root powder 1,2% dan P4= Feed with protein and calcium microparticles + bitter mustard root powder 1,2%. The parameters measured were population of  lactic acid bacteria, coliform, pH, protein and calcium intake. Data were analyzed using analysis of variance (Anova) and different test (Duncan) on 5% level. The results showed that the use of rations with protein and calcium feed ingredients in macroparticles or microparticles with the addition of bitter mustard root powder had a significant effect (p<0,05) on  population of small intestine bacteria and nutrient intake. The use of protein and calcium microparticles with the addition of 1.2% bitter mustard root powder (P4) was able to increase the population of lactic acid bacteria and nutrient intake (protein and calcium) and decreased coliform population and small intestine pH compared to other treatments (P1-P3). The conclusion is feeding of protein and calcium microparticles and addition of 1.2% bitter mustard root powder could increase the population of lactic acid bacteria, protein and calcium intake and reduce the coliform population and the pH of the small intestine.

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