scholarly journals Assessment of Antioxidant Potential, Total Phenolics and Flavonoids of Different Solvent Fractions of Monotheca Buxifolia Fruit

2013 ◽  
Vol 4 (5) ◽  
pp. 246-254 ◽  
Author(s):  
Shumaila Jan ◽  
Muhammad Rashid Khan ◽  
Umbreen Rashid ◽  
Jasia Bokhari
Keyword(s):  
Total Phenolics ◽  
Antioxidant Potential ◽  
Monotheca Buxifolia

Investigating the Antioxidant Potential of Garlic (Allium sativum) Extracts Through Different Extraction Modes

2019 ◽  
Vol 15 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Kanza A. Awan ◽  
Masood S. Butt ◽  
Iahtisham Ul Haq ◽  
Hafiz A.R. Suleria
Keyword(s):  
Allium Sativum ◽  
Total Phenolics ◽  
Methanolic Extract ◽  
Antioxidant Potential ◽  
Flavonoid Content ◽  
Time Intervals ◽  
Methanolic Extracts ◽  
Maximum Extraction ◽  
Acid Potential ◽  
The Ideal

Background: Garlic (Allium sativum) possesses health enhancing abilities due to the presence various phytoceutics moities. The current research was deigned to explore the phytochemicals and antioxidant capacity of Pakistani garlic. Methods: Garlic extracts were obtained using methanol, hexane and ethyl acetate at different time intervals (35, 50 and 65 min) followed by their polyphenols and flavonoid content determination. Afterwards, the antioxidant potential was also determined. Results: The outcomes revealed that the methanolic extracts obtained at 50 min extraction time showed maximum total phenolics as 60.38±0.23 mg GAE/100g and flavonoids as 58.45±1.24 mg/100g. Similarly, the highest DPPH activity (61.59±1.58%) and β-carotene and linoleic acid potential (64.96±1.72%) were also observed for methanolic extract. Conclusion: Inferences were made that Pakistani garlic contains myriad of phenolics and flavonoids but the extraction of these components depends upon the solvent/time combination. In this study, methanol proved to be the ideal solvent for the maximum extraction of phytochemicals from garlic.

scholarly journals Antioxidant Potential of Flower Extracts from Centaurea spp. Depends on Their Content of Phenolics, Flavonoids and Free Amino Acids

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7465
Author(s):  
Piotr Salachna ◽  
Anna Pietrak ◽  
Łukasz Łopusiewicz
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Reducing Sugars ◽  
Total Phenolics ◽  
Raw Materials ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Strong Positive Correlation ◽  
Important Health

Scientists intensely search for new sources of antioxidants, perceived as important health-promoting agents. Some species of the large genus Centaurea provide raw materials for the pharmaceutical and cosmetic industries, as well as produce edible flowers. This is the first study that determines the content of total polyphenols, flavonoids, reducing sugars, free amino acids and the antioxidant potential in the flower extracts of C. nigra L., C. orientalis L. and C. phrygia L. The total polyphenol and flavonoid content is the highest in the extract of C. orientalis, and the lowest in that of C. phrygia. Similarly, C. orientalis shows the greatest scavenging activity on DPPH (1,1-diphenyl-2-picryl-hydrazyl), ABTS [2,2′-azobis(3-ethylbenzothiazoline-6-sulfonate)] and Fe3+ reducing power assays, whereas the lowest activity is found for C. phrygia. The highest content of reducing sugars is found in C. nigra, while C. orientalis has the highest levels of free amino acids. We find a strong positive correlation between total phenolics and flavonoids and the antioxidant capacity of all three Centaurea species. Moreover, the content of free amino acids strongly and positively correlates with the levels of total phenolics and flavonoids, antioxidant activity assessed by DPPH and ABTS assays and Fe3+ reducing power. Summing up, C. orientalis exhibits the strongest antioxidant potential of the investigated Centaurea species. This species could potentially be a natural source of antioxidant substances for the pharmacy, cosmetics and food industries. The content of free amino acids may be used as a marker of the antioxidant status of Centaurea species.

scholarly journals Antioxidant Potential and Stabilization Studies of Sunflower Oil Using Sorbaria tomentosa Extract and its Cu(II)/Zn(II) Chelates

2020 ◽  
Vol 70 (12) ◽  
pp. 4193-4200
Keyword(s):  
Sunflower Oil ◽  
Total Phenolics ◽  
Storage Period ◽  
Ethanolic Extract ◽  
Antioxidant Potential ◽  
Emulsion System ◽  
Synthetic Antioxidants ◽  
Good Source ◽  
Linoleic Acid Emulsion ◽  
Β Carotene

Sorbaria tomentosa,, commonly known as “Berre” is native to Himalaya and Hindukush range in Pakistan. Qualitative phytochemical screening as well as quantitative antioxidant potential of its ethanolic extract was evaluated. Antioxidant potential of the extract was determined using standard methods like DPPH, FRAP, total phenolics, total flavonoids, ABTS radical cation scavenging assay and β-carotene linoleic acid emulsion system. Cu(II) and Zn(II) chelates of ,,Sorbaria tomentosa,, were also prepared and their antioxidant potential was compared with the extract as well as with synthetic antioxidants (BHT and BHA). It was observed that ,,Sorbaria tomentosa,, is a good source of natural antioxidant that worked efficiently compared to respective chelates. Keeping in view less efficiency of chelated extracts, stabilization studies of sunflower oil were conducted with ethanolic extract (250, 500, 1000 ppm) of ,,Sorbaria tomentosa,,. Various parameters like PV, FFA and IV were estimated to evaluate stabilization of oil. ,,Sorbaria tomentosa,, extract (1000 ppm) showed almost same role during stabilization like BHA at ambient condition during a storage period of 45 days. Keywords: Sorbaria tomentosa, phytochemicals, antioxidant potential, chelation, stabilization

scholarly journals Metabolic fingerprinting, antioxidant characterization, and enzyme-inhibitory response of Monotheca buxifolia (Falc.) A. DC. extracts

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Joham Sarfraz Ali ◽  
Hammad Saleem ◽  
Abdul Mannan ◽  
Gokhan Zengin ◽  
Mohamad Fawzi Mahomoodally ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
Methanol Extract ◽  
Biological Activities ◽  
Chloroform Extract ◽  
Inhibitory Response ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Ms Analysis ◽  
Monotheca Buxifolia

Abstract Background Ethnobotanical and plant-based products allow for the isolation of active constituents against a number of maladies. Monotheca buxifolia is used by local communities due to its digestive and laxative properties, as well as its ability to cure liver, kidney, and urinary diseases. There is a need to explore the biological activities and chemical constituents of this medicinal plant. Methods In this work, the biochemical potential of M. buxifolia (Falc.) A. DC was explored and linked with its biological activities. Methanol and chloroform extracts from leaves and stems were investigated for total phenolic and flavonoid contents. Ultrahigh-performance liquid chromatography coupled with mass spectrometry (UHPLC–MS) was used to determine secondary-metabolite composition, while high-performance liquid chromatography coupled with photodiode array detection (HPLC–PDA) was used for polyphenolic quantification. In addition, we carried out in vitro assays to determine antioxidant potential and the enzyme-inhibitory response of M. buxifolia extracts. Results Phenolics (91 mg gallic-acid equivalent (GAE)/g) and flavonoids (48.86 mg quercetin equivalent (QE)/g) exhibited their highest concentration in the methanol extract of stems and the chloroform extract of leaves, respectively. UHPLC–MS analysis identified a number of important phytochemicals, belonging to the flavonoid, phenolic, alkaloid, and terpenoid classes of secondary metabolites. The methanol extract of leaves contained a diosgenin derivative and polygalacin D, while kaempferol and robinin were most abundant in the chloroform extract. The methanol extract of stems contained a greater peak area for diosgenin and kaempferol, whereas this was true for lucidumol A and 3-O-cis-coumaroyl maslinic acid in the chloroform extract. Rutin, epicatechin, and catechin were the main phenolics identified by HPLC–PDA analysis. The methanol extract of stems exhibited significant 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical-scavenging activities (145.18 and 279.04 mmol Trolox equivalent (TE)/g, respectively). The maximum cupric reducing antioxidant capacity (CUPRAC) (361.4 mg TE/g), ferric-reducing antioxidant power (FRAP) (247.19 mg TE/g), and total antioxidant potential (2.75 mmol TE/g) were depicted by the methanol extract of stems. The methanol extract of leaves exhibited stronger inhibition against acetylcholinesterase (AChE) and glucosidase, while the chloroform extract of stems was most active against butyrylcholinesterase (BChE) (4.27 mg galantamine equivalent (GALAE)/g). Similarly, the highest tyrosinase (140 mg kojic-acid equivalent (KAE)/g) and amylase (0.67 mmol acarbose equivalent (ACAE)/g) inhibition was observed for the methanol extract of stems. Conclusions UHPLC–MS analysis and HPLC–PDA quantification identified a number of bioactive secondary metabolites of M. buxifolia, which may be responsible for its antioxidant potential and enzyme-inhibitory response. M. buxifolia can be further explored for the isolation of its active components to be used as a drug.

DNA Protection, Total Phenolics and Antioxidant Potential of the Mushroom Russula Virescens

2013 ◽  
Vol 38 (1) ◽  
pp. 6-17 ◽  
Author(s):  
Md Abul Hasnat ◽  
Mehnaz Pervin ◽  
Trishna Debnath ◽  
Beong Ou Lim
Keyword(s):  
Total Phenolics ◽  
Antioxidant Potential ◽  
Dna Protection

scholarly journals Phytochemical Screening, Phenolic Compounds and Antioxidant Activity of Biomass from Lychnis flos-cuculi L. In Vitro Cultures and Intact Plants

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 206 ◽  
Author(s):  
Michał P. Maliński ◽  
Małgorzata Anna Kikowska ◽  
Agata Soluch ◽  
Mariusz Kowalczyk ◽  
Anna Stochmal ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Plant Material ◽  
Total Phenolics ◽  
In Vitro Cultures ◽  
Antioxidant Potential ◽  
Phytochemical Screening ◽  
Triterpenoid Saponins ◽  
Intact Plants

Lychnis flos-cuculi L., a species with potential medicinal value, contains flavonoids, phenolic acids, triterpenoid saponins and ecdysteroids. In this study, the antioxidant activity of plant material of L. flos-cuculi obtained from in vitro cultures compared to that of intact plants from the natural site has been evaluated for the first time. Phytochemical screening of the in-vitro-derived material by ultra-high-performance liquid chromatography mass spectrometry (UHPLC-MS) confirmed the presence of the aforementioned metabolite classes. The aqueous methanolic extracts from in-vitro-derived plant material and the organs of intact plants were analyzed using spectrophotometric methods to quantify total phenolics, phenolic acids and flavonoids, and determine the preliminary antioxidant activity by ferric reducing antioxidant potential (FRAP) and DPPH radical scavenging activity assays. The results showed that the inflorescence (Ns-F), and flowering herb of both plants gathered from natural habitat (Ns-H) and in-vitro-derived plants from the experimental plot (ExV-H) are the materials richest in polyphenols (195.4, 113.47, 112.1 mg GAE g−1 d.w., respectively), and demonstrate the highest antioxidant activity (20.14, 11.24, and 11.46 mg AAE g−1 d.w.). The extract from callus exhibited the lowest polyphenol content and antioxidant potential. The contents of total phenolics, flavonoids and phenolic acids correlate with the results of the antioxidant capacity of L. flos-cuculi extracts.

scholarly journals HPLC AND ESI-MS ANALYSIS OF VANILLIN ANALOGUE 2-HYDROXY-4-METHOXY BENZALDEHYDE IN SWALLOW ROOT – THE INFLUENCE OF HABITAT HETEROGENEITY ON ANTIOXIDANT POTENTIAL

2019 ◽  
Vol 18 (2) ◽  
pp. 21-28
Author(s):  
Matam Pradeep ◽  
Nandini Prasad Shetty ◽  
Parvatam Giridhar
Keyword(s):  
Habitat Heterogeneity ◽  
Tamil Nadu ◽  
Total Phenolics ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Solvent System ◽  
Antioxidant Potential ◽  
Natural Habitats ◽  
Flavor Compound ◽  
Decalepis Hamiltonii

Decalepis hamiltonii Wight & Arn., is a plant species that is endemic to southern parts of India. The aim of this study is to explore the influence of habitat heterogeneity on total phenolics, flavonoids, flavor compound 2-hydroxy-4-methoxy benzaldehyde (2H4MB) and antioxidant potential of tubers. The flavor metabolite 2H4MB was quantified by HPLC using isocratic solvent system (methanol : acetonitrile : water : acetic acid 47 : 10 : 42 : 1) that indicates obvious difference in 2H4MB content of tubers with a maximum of 96.4 ±2.6 and 92.6 ±1.2 mg 100 g–1 dry weight basis (DW) in samples from B.R. Hills and Mysore area of Karnataka, followed by samples from Tirumalai Hills and Kurnool from Andhra Pradesh (89.02 ±0.9 mg 100 g–1 DW), Tamil Nadu (81.6 ±2.4 mg 100 g–1 DW) and Kerala (80.18 ±1.1 mg 100 g–1 DW) of tubers. There was variation in total phenolics, total flavonoids and 2H4MB content of root samples collected from different habitats. Also significant variation in free radical scavenging potential of methanol root extracts was noticed, which is directly proportional to the phenolics, and flavonoids content. Overall, there was 10–16% difference in content of 2H4MB in D. hamiltonii tubers that were collected from different natural habitats, and this habitat heterogeneity has to be considered vital, while using such tubers for edible purposes and food formulations.

scholarly journals From Harvest To Consumer: Are Grape's Health-Beneficial Properties Modified With Time In Storage?

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 427-427
Author(s):  
Lima Marta ◽  
Sideman Rebecca ◽  
Chandrakala Annasamy ◽  
Hanlon Molly
Keyword(s):  
Cold Storage ◽  
Fruits And Vegetables ◽  
Total Phenolics ◽  
Titratable Acidity ◽  
Training System ◽  
Antioxidant Potential ◽  
Post Harvest ◽  
Training Systems ◽  
Table Grapes ◽  
Cold Hardy

Abstract Objectives Currently, the intake of fruits and vegetables is lower than the recommended amounts, despite the known association between consumption of plant foods and prevention of several chronic diseases. Grapes are a widely accepted and consumed fruit, and one of the richest in phytochemicals that are beneficial to human health. Previously, we reported that growing cold hardy table grapes on two different grapevine training systems, Modified Munson (MM) and Vertical Shoot Positioning (VSP), modulates their amount of total phenolics and antioxidant potential at harvest. However, consumers access to fruit is usually not immediate upon harvest, but could take weeks/months before reaching stores. The objective of this study was to evaluate whether phenolics and antioxidant potential of table grapes grown on different training systems could be maintained post-harvest in cold storage. Methods Cold-hardy table grapes variety Mars were grown on two grapevine training systems, MM and VSP. At harvest, randomly picked grape clusters from each training system were placed in clamshells (2 clusters per clamshell) and placed in cold storage (0 ± 1°C). After 1, 2 and 3 weeks post-harvest, grapes (triplicate clamshells) were homogenized and Brix, titratable acidity, antioxidant potential and total phenolics were analyzed. Statistical analysis was performed using 2-way ANOVA. Results Total phenolics were greater for MM compared with VSP (MM 2.56 vs. VSP 1.84 mM Catechin equivalents; P = 0.0001), but were reduced in cold storage irrespective of training system (P = 0.0002). The MM training system had greater Brix (MM 17.9 vs. VSP 15.8°Bx; P < 0.0001) and antioxidant potential (MM 1.24 vs. VSP 1.01 mM Fe2+ Equivalents; P = 0.0379) than VSP system. The results also showed an effect of training system x time interaction (P = 0.0371) on titratable acidity. Conclusions Our data suggest that greater total phenolics, antioxidant potential and Brix associated with the MM compared with VSP training system are maintained in the post-harvest period. Our data also suggest that nutritional properties of grapes is reduced post-harvest, despite being in cold storage. Thus, grapevine training system and cold-storage play a role in producing grapes with increased health-beneficial properties. Funding Sources USDA NIFA Hatch.

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