Biological indicators to estimate the prevalence of gray mold and hairline cracks on table grapes cv. Thompson Seedless after cold storage

Potassium sorbate, a program of four fungicides, or one of three chitosan formulations were applied to clusters of ‘Thompson Seedless’ grape berries at berry set, pre-bunch closure, veraison, and 2 or 3 weeks before harvest. After storage at 2°C for 6 weeks, the natural incidence of postharvest gray mold was reduced by potassium sorbate, the fungicide program, or both together in a tank mixture, in 2009 and 2010. In 2011, the experiment was repeated with three chitosan products (OII-YS, Chito Plant, and Armour-Zen) added. Chitosan or fungicide treatments significantly reduced the natural incidence of postharvest gray mold among grape berries. Berries harvested from vines treated by two of the chitosan treatments or the fungicide program had fewer infections after inoculation with Botrytis cinerea conidia. None harmed berry quality and all increased endochitinase activity. Chitosan decreased berry hydrogen peroxide content. One of the chitosan formulations increased quercetin, myricetin, and resveratrol content of the berry skin. In another experiment, ‘Princess Seedless’ grape treated with one of several fungicides before 4 or 6 weeks of cold storage had less decay than the control. Fenhexamid was markedly superior to the other fungicides for control of both the incidence and spread of gray mold during storage.

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Control of postharvest gray mold of ‘BRS Nubia’ table grape under cold storage

Semina Ciências Agrárias ◽

10.5433/1679-0359.2020v41n6supl2p3457 ◽

2020 ◽

Vol 41 (6supl2) ◽

pp. 3457-3465

Author(s):

Ronan Carlos Colombo ◽

◽

Deived Uilian de Carvalho ◽

Maria Aparecida da Cruz ◽

Ciro Hideki Sumida ◽

...

Keyword(s):

Shelf Life ◽

Cold Storage ◽

Room Temperature ◽

Gray Mold ◽

Soluble Solids ◽

Experimental Unit ◽

Table Grapes ◽

Randomized Design ◽

Commercial Vineyard ◽

Solids Content

The demand for high-quality nutritional products has increased fruit consumption, as grapes, for this reason postharvest techniques are required to prevent losses, to preserve quality, to extend shelf life, and to attend to consumer needs. In this way, the objective of this study was to evaluate strategies to control gray mold caused by Botrytis cinerea in ‘BRS Nubia’ grapes during cold storage and shelf life periods. Grape bunches were harvested from a commercial vineyard in Marialva, Parana, Brazil. Grapes were subjected to the following treatments: cold storage at 2 ºC (control), cold storage at 2 ºC with SO2-generating pads, cold storage at 2 ºC and inoculated with B. cinerea suspension, and cold storage at 2 ºC with SO2-generating pads and inoculated with B. cinerea suspension. The experiment was conducted in a complete randomized design with five replications per treatment using four bunches per experimental unit. A factorial arrangement (absence/presence of SO2 pads × absence/presence of Botrytis inoculation) was applied. At the end of 30 days of cold storage and 7 days of shelf life (22 ºC), gray mold incidence, shattered berries, and physicochemical parameters were evaluated. The gray mold incidence on ‘BRS Nubia’ grapes decreased when SO2-generating pads were used during cold storage. Berry weight loss was greater in the treatments without SO2-generating pads after 30 days of cold storage followed by 7 days of shelf life. Berry firmness, soluble solids content (SS), total acidity (TA), SS/TA ratio, and anthocyanins concentration were not negatively affected by SO2-generating pad treatments. However, a slight increase in the shattered berries percentage was recorded for the SO2-generating pad treatments. No significant quality loss of ‘BRS Nubia’ grape was evident after 30 days of cold storage followed by 7 days of exposure at room temperature. In this context, SO2-generating pads can be used to control the gray mold incidence on ‘BRS Nubia’ table grapes during cold storage.

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Control of Gray Mold on Clamshell-Packaged ‘Benitaka’ Table Grapes Using Sulphur Dioxide Pads and Perforated Liners

Pathogens ◽

10.3390/pathogens8040271 ◽

2019 ◽

Vol 8 (4) ◽

pp. 271 ◽

Cited By ~ 3

Author(s):

Osmar Jose Chaves Junior ◽

Khamis Youssef ◽

Renata Koyama ◽

Saeed Ahmed ◽

Allan Ricardo Dominguez ◽

...

Keyword(s):

Mass Loss ◽

Cold Storage ◽

Storage Period ◽

Storage Conditions ◽

International Markets ◽

Gray Mold ◽

Additional Treatment ◽

Table Grapes ◽

Randomized Design ◽

Dual Release

The use of vented clamshells has become popular in the packaging of grapes for local and international markets. The aim of this study is to evaluate the postharvest preservation of ‘Benitaka’ table grapes individually packaged in vented clamshells using different types of SO2-generating pads and perforated plastic liners during cold storage. A completely randomized design with four replications in a two-factor arrangement with an additional treatment [(4 × 3) + 1] was used. The trials were carried out under two situations: Artificial or natural infections with Botrytis cinerea, which is the causal agent of gray mold on table grapes. The incidence of gray mold, shattered berries, and stem browning were evaluated at 30 and 45 days of cold storage at 1 ± 1 °C and 3 days of shelf-life at 22 ± 1 °C after the period of cold storage. Mass loss and berry firmness were also examined at the end of the cold storage period. The use of dual-release SO2-generating pads containing 5 or 8 g of a.i. and slow-release pads with 7 g of a.i. was effective in controlling the incidence of gray mold in grapes packaged in vented clamshells and kept under cold storage for up to 45 days. Under these storage conditions, perforated plastic liners with 0.3% ventilation area or micro-perforated liners with 1.0% ventilation area reduced the percentage of mass loss and shattered berries.

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BiofumigationwithMuscodoralbus Pads for Controlling Decay in Commercial Table Grape Cartons

HortScience ◽

10.21273/hortsci.40.4.1144d ◽

2005 ◽

Vol 40 (4) ◽

pp. 1144D-1144 ◽

Cited By ~ 4

Author(s):

Julien Mercier ◽

Paul Walgenbach ◽

Jorge I. Jiménez

Keyword(s):

Isobutyric Acid ◽

Gray Mold ◽

Fruit Weight ◽

Table Grape ◽

Fungal Decay ◽

Isobutyl Alcohol ◽

Thompson Seedless ◽

Significant Control ◽

Table Grapes ◽

And Control

The volatile-producing fungus Muscodoralbus is being developed as a biological fumigant for postharvest use, as it can kill storage pathogens and control fungal decay in various commodities. A wettable pad or sachet system made of teabag paper containing desiccated rye grain culture of M. albus was designed for the biofumigation of individual fruit containers. The fungus is reactivated before use by a brief immersion of the pad in water. This research was conducted to determine the potential of the pad system for controlling decay of table grapes in commercial cartons. Individual pads containing 24 or 86 g of grain culture (to achieve a 1:10 ratio of fumigant to box volume or a 1:100 ratio of fumigant to fruit weight, respectively) were added to Styrofoam cartons containing 8.6 kg of freshly harvested `Thompson Seedless' (TS) or `Ruby Seedless' (RS) grapes, which were then placed in cold storage at –1 to 0 °C. Control cartons exposed to SO2 were placed in a separate storage room and SO2 fumigation was performed once for TS and weekly for RS. After 8 to 9 weeks, the grapes were taken out of storage and rated for decay. In the experiment with TS, the 24-g and 86-g pads provided significant control of gray mold rot when compared to untreated cartons and were not statistically different from cartons exposed to a single SO2 fumigation. In the experiment with RS, only the 86-g pads provided significant decay control. Measurements of the three most abundant volatile compounds in empty cartons containing 10 g of the biofumigant revealed that partial coverage of holes mimicking obstruction by packed fruit achieved levels of isobutyl alcohol, 2-methyl-1-butanol, and isobutyric acid of 0.7, 1.6, and 11.2 ppb, respectively.

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Effects of Modified Atmosphere Packaging and Chitosan Treatment on Quality and Sensorial Parameters of Minimally Processed cv. ‘Italia’ Table Grapes

Agronomy ◽

10.3390/agronomy11020328 ◽

2021 ◽

Vol 11 (2) ◽

pp. 328

Author(s):

Giorgia Liguori ◽

Giuseppe Sortino ◽

Gregorio Gullo ◽

Paolo Inglese

Keyword(s):

Cold Storage ◽

Modified Atmosphere Packaging ◽

Gray Mold ◽

Table Grape ◽

Modified Atmosphere ◽

Minimally Processed ◽

High Co2 ◽

Table Grapes ◽

Climacteric Fruit ◽

Negative Effect

Table grape is a non-climacteric fruit, very sensitive to water loss and gray mold during postharvest handling and storage. The aim of this work was to evaluate the effects of modified atmosphere packaging and chitosan treatment on quality and sensorial parameters of minimally processed cv. ‘Italia’ table grape during cold storage (14 days at 5 °C) and shelf-life (7 and 14 days of cold storage plus 5 days at 20 °C), reproducing a retail sales condition. Our data showed a significant effect of high CO2-modified atmosphere in combination with chitosan and alone on preserving quality, sensorial parameters, and delaying decay of minimally processed table grape. The most effective treatment in terms of preservation of quality, sensory, and nutritional quality was high-CO2 modified atmosphere packaging plus chitosan treatment. The overall results showed that when a proper modified atmosphere packaging is combined with a postharvest chitosan treatment, gray mold growth can effectively be controlled in ready-to-eat cv. ‘Italia’ table grape and that modified atmosphere packaging (MAP) with high levels of CO2 and reduced concentration of O2 in combination with chitosan treatment could prevent the negative effect of high-CO2 in-packages level, like berry browning and rachis browning and dehydration.

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Identification of epiphytic yeasts and bacteria with potential for biocontrol of grey mold disease on table grapes caused by Botrytis cinerea

Spanish Journal of Agricultural Research ◽

10.5424/sjar/2018161-11378 ◽

2018 ◽

Vol 16 (1) ◽

pp. e1002 ◽

Cited By ~ 4

Author(s):

Kazem Kasfi ◽

Parissa Taheri ◽

Behrooz Jafarpour ◽

Saeed Tarighi

Keyword(s):

Botrytis Cinerea ◽

Disease Incidence ◽

Gray Mold ◽

Antagonistic Activity ◽

Antagonistic Effect ◽

Bacterial Isolates ◽

Thompson Seedless ◽

Grape Berries ◽

Table Grapes

The objective of this study was to identify grapevine epiphytic yeasts and bacteria for biocontrol of Botrytis cinerea on grapes. Antagonistic yeasts and bacteria were isolated from the epiphytic flora associated with grape berries and leaves cv. ‘Thompson seedless’ from vineyards in Iran and identified by sequencing the conserved genomic regions. A total of 130 yeast and bacterial isolates from the surface of grapevine were screened in vitro for determining their antagonistic effect against B. cinerea and used to control postharvest gray mold. Among the 130 isolates, five yeasts and four bacterial isolates showed the greatest antagonistic activity in vitro against B. cinerea. Two yeasts species including Meyerozyma guilliermondii and Candida membranifaciens had high antagonistic capability against the pathogen. Also, 4 bacterial isolates belonging to Bacillus sp. and Ralstonia sp. showed significant biocontrol effect against B. cinerea. The isolates were capable of producing volatile and non-volatile substances, which suppressed the pathogen growth. The antagonistic activity of selected yeasts and bacteria against the pathogen was investigated on wounded berries of ‘Thompson seedless’. On small clusters with intact berries, all of the antagonistic isolates considerably reduced the decay on grape berries and inhibition of gray mold incidence on fruits treated by these isolates was less than 50%, except for the isolate N1, which had higher capability in inhibiting the disease incidence. These results suggest that antagonist yeasts and bacteria with potential to control B. cinerea on grape can be found in the microflora of grape berries and leaves.

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Efficacy of essential oils against gray mold and effect on fruit quality during cold storage in table grapes

South African Journal of Botany ◽

10.1016/j.sajb.2021.11.046 ◽

2022 ◽

Vol 146 ◽

pp. 481-490

Author(s):

Mohamed I. Elsayed ◽

Adel D. Al-Qurashi ◽

Najeeb Marei Almasaudi ◽

Kamal A.M Abo-Elyousr

Keyword(s):

Essential Oils ◽

Fruit Quality ◽

Cold Storage ◽

Gray Mold ◽

Table Grapes

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Preharvest Chitosan and Postharvest UV Irradiation Treatments Suppress Gray Mold of Table Grapes

Plant Disease ◽

10.1094/pd-90-0445 ◽

2006 ◽

Vol 90 (4) ◽

pp. 445-450 ◽

Cited By ~ 81

Author(s):

G. Romanazzi ◽

F. Mlikota Gabler ◽

J. L. Smilanick

Keyword(s):

Chitinase Activity ◽

Gray Mold ◽

Grape Berry ◽

Thompson Seedless ◽

Berry Skin ◽

Grape Berries ◽

Table Grapes ◽

Ultraviolet C ◽

Berry Skins ◽

Uv C

The effectiveness of chitosan treatment of table grapes, alone or in combination with ultraviolet-C (UV-C) radiation, to control postharvest gray mold caused by Botrytis cinerea, was determined in California, United States. The influence of these treatments on catechin and resveratrol contents and chitinase activity in grape berry skins also was assessed. Clusters of cvs. Thompson Seedless, Autumn Black, and Emperor were sprayed in the vineyard with 1% chitosan, then harvested daily for 5 days. Promptly after harvest, they were inoculated with B. cinerea. Decay incidence and disease severity were significantly reduced by chitosan, which was most effective on berries harvested 1 or 2 days after treatment. In another experiment, grape berries were sprayed in the vineyard with chitosan, harvested 2 days later, irradiated for 5 min with UV-C (0.36 J/cm2), and inoculated with B. cinerea 2 days later. Combined chitosan and UV-C treatments applied to cv. Autumn Black or selection B36-55 were synergistic in reducing gray mold incidence and severity compared with either treatment alone. Preharvest chitosan treatment increased neither concentration of catechin or resveratrol nor activity of chitinase in berry skin. Conversely, UV-C irradiation, alone or combined with chitosan treatment, induced catechin in cv. Autumn Black berries and trans-resveratrol in both cv. Autumn Black and selection B36-55.

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Control of Postharvest Gray Mold of Table Grapes in the San Joaquin Valley of California by Fungicides Applied During the Growing Season

Plant Disease ◽

10.1094/pdis-94-2-0250 ◽

2010 ◽

Vol 94 (2) ◽

pp. 250-257 ◽

Cited By ~ 25

Author(s):

J. L. Smilanick ◽

M. F. Mansour ◽

F. Mlikota Gabler ◽

D. A. Margosan ◽

J. Hashim-Buckey

Keyword(s):

Growing Season ◽

Gray Mold ◽

Thompson Seedless ◽

Fungicide Sensitivity ◽

Air Blast ◽

Thiophanate Methyl ◽

Table Grapes ◽

Current Maximum ◽

And Storage ◽

Berry Set

Fungicides applied before harvest were evaluated to control postharvest gray mold of table grapes, caused by Botrytis cinerea. The concentrations of thiophanate methyl (THM), iprodione (IPR), cyprodinil (CYP), pyraclostrobin + boscalid (PS+BO), pyrimethanil (PYR), or fenhexamid (FEN) that inhibited the growth of four isolates sensitive to these fungicides by 50% (EC50) were 12.4, 2.5, 0.61, 0.29/0.57, 0.26, or 0.17 mg liter-1, respectively. THM, IPR, CYP, PS+BO, PYR, or FEN were applied to detached ‘Thompson Seedless’ berries at the equivalent of the maximum approved rates of 600, 500, 270, 59/116, 370, or 290 mg liter-1, respectively, except PS+BO, which were used at 54.2% of their current registered maximum rates. The berries were inoculated with B. cinerea 48 or 24 h before treatment or 24 or 48 h after treatment. Gray mold 2 weeks after treatment and storage at 15°C was lowest after FEN application, followed by PYR, CYP, IPR, PS+BO, and THM. In commercial vineyards, one application of FEN, PYR, CYP, or PS+BO, all at their current maximum approved rates, 2 weeks before harvest reduced postharvest gray mold by approximately 50%. When fungicides were applied repeatedly after berry set either in mixtures or alternated with fungicides of different mode of action classes, postharvest gray mold was reduced by about 50% using a commercial air-blast sprayer and by 70 to 87% using a hand-held sprayer that was directed into the clusters. The fungicide sensitivity of isolates collected in numerous vineyards indicated those with reduced sensitivity to all of the tested fungicides, except FEN, were common. The efficacy of preharvest fungicide regimes was not sufficient to replace postharvest sulfur dioxide fumigation.

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Cold Storage and Biocontrol Agents to Extend the Storage Period of ‘BRS Isis’ Seedless Table Grapes

Horticulturae ◽

10.3390/horticulturae4030018 ◽

2018 ◽

Vol 4 (3) ◽

pp. 18 ◽

Cited By ~ 1

Author(s):

Ronan Colombo ◽

Deived Carvalho ◽

Maria Cruz ◽

Ciro Sumida ◽

Saeed Ahmed ◽

...

Keyword(s):

Cold Storage ◽

Water Loss ◽

Biological Control Agent ◽

Storage Period ◽

Titratable Acidity ◽

Gray Mold ◽

Soluble Solids ◽

Postharvest Disease ◽

Table Grapes ◽

Seedless Grape

‘BRS Isis’ is a novel hybrid seedless table grape with large reddish bunches. This cultivar is cultivated in tropical and subtropical areas and has potential to be exported overseas; however, under these growth conditions, grapes can be severely damaged by gray mold (Botrytis cinerea), the main postharvest disease of table grapes. Thus, this study aimed to evaluate different pre- and postharvest strategies to extend the storage period of ‘BRS Isis’ seedless table grapes. The treatments consisted of grapes packed into carton boxes under cold storage (1 °C and high relative humidity), pre- and/or postharvest treatment with potassium bicarbonate or a biological control agent (Bacillus subtilis), all with Botrytis inoculation. Additionally, two controls, with and without Botrytis inoculation, were also evaluated. The experimental design was completely randomized with eight treatments and three replications, and each plot consisted of four bunches. After a 50-day cold storage period, the carton boxes were kept for five days at room temperature (22 °C). Gray mold incidence and water loss, as well as soluble solids (SS), pH, titratable acidity (TA), SS/TA ratio, and color attributes were evaluated during both periods. Cold storage effectively reduced gray mold in ‘BRS Isis’ seedless grape for a period of 50 days, even when grapes were inoculated; however, no difference among treatments was observed. Higher water loss was observed in both non-treated control treatments.

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