scholarly journals Immunology, Health and Disease The effects of dietary supplementation with lotus leaf extract on the immune response, and intestinal microbiota composition of broiler chickens

2020 ◽  
Author(s):  
Lei Cheng ◽  
Wei Zhang ◽  
Qing Jin ◽  
Yiling Zhu ◽  
Rong Chen ◽  
...  
Keyword(s):  
Immune Response ◽  
Intestinal Microbiota ◽  
Broiler Chickens ◽  
Leaf Extract ◽  
Dietary Supplementation ◽  
Microbiota Composition ◽  
Lotus Leaf ◽  
Health And Disease

Effects of feeding Isomaltooligosaccharides on the growth performance, carcass traits and immune response of broiler chickens

2014 ◽  
Vol 3 (1) ◽  
pp. 150-157
Author(s):  
Khalid M. Gaafar
Keyword(s):  
Immune Response ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Carcass Traits ◽  
Dietary Supplementation ◽  
Control Group ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Flu Vaccine ◽  
Lipids Profile

The research was conducted to study the effect of feeding broiler chickens on diets containing isomaltooligosaccharides on the growth performance, carcass traits and immune response. 90-one day old broiler chicks were used according to completely randomized two treatment groups and one control, 30 birds each. Birds fed ad-libitum on basal starter and grower-finisher diets for 35 day. Diets of treatment`s groups contained 0.5 g/Kg and 1 g/Kg of Isomaltooligosaccharides, while the control group fed on the basal diets without Isomaltooligosaccharides supplementation. Dietary supplementation of broiler chickens with Isomaltooligosaccharides improved body weight, feed conversion, carcass traits, two lymphoid organs weight and log antibody titer against avian flu vaccine. Most of the highest values were for birds fed low levels of Isomaltooligosaccharides. Feed intake decreases as Isomaltooligosaccharides level increases. Dietary supplementation with Isomaltooligosaccharides did not affect the lipids profile (triglycerides, total cholesterol, LDL and HDL), however the blood VLDL levels decreased with increased levels of Malondialdehyde and Glutathione reductase. Collectively, Dietary supplementation of broiler chickens with 0.5 g/Kg diet of Isomaltooligosaccharides improved growth performance, carcass traits and immune status.

scholarly journals Comparison of Different Invasive and Non-Invasive Methods to Characterize Intestinal Microbiota throughout a Production Cycle of Broiler Chickens

2019 ◽  
Vol 7 (10) ◽  
pp. 431 ◽  
Author(s):  
Jannigje Kers ◽  
Egil Fischer ◽  
J. Stegeman ◽  
Hauke Smidt ◽  
Francisca Velkers
Keyword(s):  
Longitudinal Data ◽  
Intestinal Microbiota ◽  
Broiler Chickens ◽  
Amplicon Sequencing ◽  
Production Cycle ◽  
Microbiota Composition ◽  
Cecal Content ◽  
Non Invasive ◽  
Longitudinal Data Collection ◽  
Similar Development

In the short life of broiler chickens, their intestinal microbiota undergoes many changes. To study underlying biological mechanisms and factors that influence the intestinal microbiota development, longitudinal data from flocks and individual birds is needed. However, post-mortem collection of samples hampers longitudinal data collection. In this study, invasively collected cecal and ileal content, cloacal swabs collected from the same bird, and boot sock samples and cecal droppings from the litter of the broilers’ poultry house, were collected on days 0, 2, 7, 14 and 35 post-hatch. The different sample types were evaluated on their applicability and reliability to characterize the broiler intestinal microbiota. The microbiota of 247 samples was assessed by 16S ribosomal RNA gene amplicon sequencing. Analyses of α and β measures showed a similar development of microbiota composition of cecal droppings compared to cecal content. Furthermore, the composition of cecal content samples was comparable to that of the boot socks until day 14 post-hatch. This study shows that the value of non-invasive sample types varies at different ages and depends on the goal of the microbiota characterization. Specifically, cecal droppings and boot socks may be useful alternatives for cecal samples to determine intestinal microbiota composition longitudinally.

scholarly journals Supplemental Bacillus subtilis DSM 29784 and enzymes, alone or in combination, as alternatives for antibiotics to improve growth performance, digestive enzyme activity, anti-oxidative status, immune response and the intestinal barrier of broiler chickens

2020 ◽  
pp. 1-14 ◽  
Author(s):  
Yuanyuan Wang ◽  
Chianning Heng ◽  
Xihong Zhou ◽  
Guangtian Cao ◽  
Lei Jiang ◽  
...  
Keyword(s):  
Immune Response ◽  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Growth Performance ◽  
Intestinal Microbiota ◽  
Broiler Chickens ◽  
Digestive Enzyme ◽  
Intestinal Barrier ◽  
Digestive Enzyme Activity ◽  
Jejunal Mucosa

Abstract The present study investigated the effect of Bacillus subtilis DSM 29784 (Ba) and enzymes (xylanase and β-glucanases; Enz), alone or in combination (BE) as antibiotic replacements, on the growth performance, digestive enzyme activity, immune response and the intestinal barrier of broiler chickens. In total, 1200 1-d-old broilers were randomly assigned to five dietary treatments, each with six replicate pens of forty birds for 63 d as follows: (a) basal diet (control), supplemented with (b) 1 × 109 colony-forming units (cfu)/kg Ba, (c) 300 mg/kg Enz, (d) 1 × 109 cfu/kg Ba and 300 mg/kg Enz and (e) 250 mg/kg enramycin (ER). Ba, Enz and BE, similar to ER, decreased the feed conversion rate, maintained intestinal integrity with a higher villus height:crypt depth ratio and increased the numbers of goblet cells. The BE group exhibited higher expression of claudin-1 and mucin 2 than the other four groups. BE supplementation significantly increased the α-diversity and β-diversity of the intestinal microbiota and markedly enhanced lipase activity in the duodenal mucosa. Serum endotoxin was significantly decreased in the BE group. Compared with those in the control group, increased superoxide dismutase and glutathione peroxidase activities were observed in the jejunal mucosa of the Ba and BE groups, respectively. In conclusion, the results suggested that dietary treatment with Ba, Enz or BE has beneficial effects on growth performance and anti-oxidative capacity, and BE had better effects than Ba or Enz alone on digestive enzyme activity and the intestinal microbiota. Ba or Enz could be used as an alternative to antibiotics for broiler chickens.

scholarly journals Bacillus subtilis DSM 32315 Supplementation Attenuates the Effects of Clostridium perfringens Challenge on the Growth Performance and Intestinal Microbiota of Broiler Chickens

2019 ◽  
Vol 7 (3) ◽  
pp. 71 ◽  
Author(s):  
Cristiano Bortoluzzi ◽  
Bruno Serpa Vieira ◽  
Juliano Cesar de Paula Dorigam ◽  
Anita Menconi ◽  
Adebayo Sokale ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Clostridium Perfringens ◽  
Intestinal Microbiota ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Dietary Supplementation ◽  
Necrotic Enteritis ◽  
Feed Conversion ◽  
Negative Effects ◽  
Infected Control

The objective of this study was to evaluate the effects of the dietary supplementation of Bacillus subtilis DSM 32315 (probiotic) on the performance and intestinal microbiota of broiler chickens infected with Clostridium perfringens (CP). One-day-old broiler chickens were assigned to 3 treatments with 8 replicate pens (50 birds/pen). The treatments were: non-infected control; infected control; and infected supplemented with probiotic (1 × 106 CFU/g of feed). On day of hatch, all birds were sprayed with a coccidia vaccine based on the manufacturer recommended dosage. On d 18–20 the infected birds were inoculated with CP via feed. Necrotic enteritis (NE) lesion score was performed on d 21. Digestive tract of 2 birds/pen was collected on d 21 to analyze the ileal and cecal microbiota by 16S rRNA sequencing. Performance was evaluated on d 28 and 42. On d 21, probiotic supplementation reduced (p < 0.001) the severity of NE related lesion versus infected control birds. On d 28, feed efficiency was improved (p < 0.001) in birds supplemented with probiotic versus infected control birds. On d 42, body weight gain (BW gain) and feed conversion ratio (FCR) were improved (p < 0.001) in probiotic supplemented birds versus infected control birds. The diversity, composition and predictive function of the intestinal microbial digesta changed with the infection but the supplementation of probiotic reduced these variations. Therefore, dietary supplementation of Bacillus subtilis DSM 32315 was beneficial in attenuating the negative effects of CP challenge on the performance and intestinal microbiota of broilers chickens.

scholarly journals The Effect of Dietary Mushroom Agaricus bisporus on Intestinal Microbiota Composition and Host Immunological Function

Nutrients ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1721 ◽  
Author(s):  
Gloria Solano-Aguilar ◽  
Saebyeol Jang ◽  
Sukla Lakshman ◽  
Richi Gupta ◽  
Ethiopia Beshah ◽  
...  
Keyword(s):  
Immune Response ◽  
Intestinal Microbiota ◽  
Mononuclear Cells ◽  
Host Immune Response ◽  
Proximal Colon ◽  
Microbiota Composition ◽  
Peripheral Blood Mononuclear ◽  
Linear Discriminant ◽  
Freeze Dried ◽  
And Function

A study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function. Thirty-one six-week-old pigs were fed a pig grower diet alone or supplemented with either three or six servings of freeze-dried white button (WB)-mushrooms for six weeks. Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with Salmonella typhymurium-Lipopolysaccharide (LPS). Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function. Pigs gained weight with no difference in body composition or intestinal permeability. Feeding mushrooms reduced LPS-induced IL-1β gene expression in AM (P < 0.05) with no change in LPS-stimulated PBMC or the intestinal mucosa transcriptome. LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs. These results suggested that feeding WB mushrooms significantly reduced the LPS-induced inflammatory response in AM and positively modulated the host microbiota metabolism by increasing the abundance of Clostridiales taxa that are associated with improved intestinal health.

scholarly journals Effects of inulin and di-d-fructose dianhydride-enriched caramels on intestinal microbiota composition and performance of broiler chickens

animal ◽  
2013 ◽  
Vol 7 (11) ◽  
pp. 1779-1788 ◽  
Author(s):  
M.J. Peinado ◽  
A. Echávarri ◽  
R. Ruiz ◽  
E. Suárez-Pereira ◽  
C. Ortiz Mellet ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Broiler Chickens ◽  
Microbiota Composition ◽  
And Performance

Effects of β-mannanase supplementation on the intestinal microbiota composition of broiler chickens challenged with a coccidiosis vaccine

2019 ◽  
Vol 228 ◽  
pp. 187-194 ◽  
Author(s):  
C. Bortoluzzi ◽  
L.B. Scapini ◽  
M.V. Ribeiro ◽  
M.R. Pivetta ◽  
R. Buzim ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Broiler Chickens ◽  
Microbiota Composition
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