Pseudomonas fluorescens metabolites as biopriming agent for systemic resistance induction in tomato against Fusarium wilt

Rhizosphere ◽  
2019 ◽  
Vol 11 ◽  
pp. 100168
Author(s):  
Abdulrahman Ali Alzandi ◽  
Deyala M. Naguib
Keyword(s):  
Pseudomonas Fluorescens ◽  
Fusarium Wilt ◽  
Systemic Resistance ◽  
Resistance Induction

scholarly journals Tea Tree Oil Induces Systemic Resistance against Fusarium wilt in Banana and Xanthomonas Infection in Tomato Plants

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1137
Author(s):  
Ronaldo J. D. Dalio ◽  
Heros J. Maximo ◽  
Rafaela Roma-Almeida ◽  
Janaína N. Barretta ◽  
Eric M. José ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Priming Effect ◽  
Xanthomonas Campestris ◽  
Plant Pathogens ◽  
Systemic Resistance ◽  
Marker Genes ◽  
Tea Tree Oil ◽  
Tomato Plants ◽  
Resistance Induction ◽  
Tea Tree

The essential tea tree oil (TTO) derived from Melaleuca alternifolia plant is widely used as a biopesticide to protect crops from several plant-pathogens. Its activity raised queries regarding its ability to, not only act as a bio-fungicide or bio-bactericide, but also systemically inducing resistance in plants. This was examined by TTO application to banana plants challenged by Fusarium oxysporum f. sp. cubense (Foc, Race 1) causing Fusarium wilt and to tomato plants challenged by Xanthomonas campestris. Parameters to assess resistance induction included: disease development, enzymatic activity, defense genes expression correlated to systemic acquired resistance (SAR) and induced systemic resistance (ISR) and priming effect. Spraying TTO on field-grown banana plants infected with Foc and greenhouse tomato plants infected with Xanthomonas campestris led to resistance induction in both hosts. Several marker genes of salicylic acid, jasmonic acid and ethylene pathways were significantly up-regulated in parallel with symptoms reduction. For tomato plants, we have also recorded a priming effect following TTO treatment. In addition to fungicidal and bactericidal effect, TTO can be applied in more sustainable strategies to control diseases by enhancing the plants ability to defend themselves against pathogens and ultimately diminish chemical pesticides applications.

scholarly journals Activity of plant growth promoting rhizobacteria (PGPRs) in the biocontrol of tomato Fusarium wilt

2017 ◽  
Vol 53 (No. 2) ◽  
pp. 78-84 ◽  
Author(s):  
Boukerma Lamia ◽  
Benchabane Messaoud ◽  
Charif Ahmed ◽  
Khélif Lakhdar
Keyword(s):  
Fusarium Wilt ◽  
Plant Growth Promoting Rhizobacteria ◽  
Significant Inhibition ◽  
Systemic Resistance ◽  
In Situ Experiment ◽  
Fluorescent Pseudomonas ◽  
Split Root ◽  
Plant Growth Promoting ◽  
Severity Of The Disease

The potential of Pseudomonas fluorescens PF15 and Pseudomonas putida PP27 to protect tomato plants against Fusarium wilt under greenhouse conditions was evaluated. In vitro antagonism showed a significant inhibition of the pathogen growth (47%) revealed by PF15. However, PP27 presented a 10% rate of the mycelium inhibition. An in situ experiment was conducted with split-root design for induced systemic resistance (ISR) and without split-root design to measure both ISR and antagonistic activities. Fluorescent Pseudomonas revealed a delay in the onset of symptoms and slower kinetics of disease progression compared to the pathogen control. McKinney’s index, which measures the severity of the disease, was reduced by 37–72%, and the levels of infection (incidence) by 7–36%.

scholarly journals Perception of Biocontrol Potential of Bacillusinaquosorum KR2-7 against Tomato Fusarium Wilt through Merging Genome Mining with Chemical Analysis

Biology ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 137
Author(s):  
Maedeh Kamali ◽  
Dianjing Guo ◽  
Shahram Naeimi ◽  
Jafar Ahmadi
Keyword(s):  
Chemical Analysis ◽  
Plant Growth ◽  
Fusarium Wilt ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Genome Mining ◽  
Gene Clusters ◽  
Systemic Resistance ◽  
Plant Colonization ◽  
Biocontrol Potential

Tomato Fusarium wilt, caused by Fusarium oxysporum f. sp. lycopersici (Fol), is a destructive disease that threatens the agricultural production of tomatoes. In the present study, the biocontrol potential of strain KR2-7 against Fol was investigated through integrated genome mining and chemical analysis. Strain KR2-7 was identified as B. inaquosorum based on phylogenetic analysis. Through the genome mining of strain KR2-7, we identified nine antifungal and antibacterial compound biosynthetic gene clusters (BGCs) including fengycin, surfactin and Bacillomycin F, bacillaene, macrolactin, sporulation killing factor (skf), subtilosin A, bacilysin, and bacillibactin. The corresponding compounds were confirmed through MALDI-TOF-MS chemical analysis. The gene/gene clusters involved in plant colonization, plant growth promotion, and induced systemic resistance were also identified in the KR2-7 genome, and their related secondary metabolites were detected. In light of these results, the biocontrol potential of strain KR2-7 against tomato Fusarium wilt was identified. This study highlights the potential to use strain KR2-7 as a plant-growth promotion agent.

scholarly journals Plant growth-promoting rhizobacteria induced resistance in Jatropha curcas through phenyl propanoid metabolism against Rhizoctoniabataticola

2017 ◽  
Vol 9 (1) ◽  
pp. 121-128
Author(s):  
S. Kumar ◽  
M. Singh ◽  
Sushil Sharma
Keyword(s):  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Jatropha Curcas ◽  
Root Rot ◽  
Plant Growth Promoting Rhizobacteria ◽  
Systemic Resistance ◽  
Total Phenols ◽  
Phenyl Propanoid ◽  
Plant Growth Promoting ◽  
Growth Promoting

The root rot disease in Jatropha curcas L. caused by Rhizoctonia. bataticola (Taub.) Butler has been recorded in causing 10-12 per cent mortality of 20-30 days old seedlings of Jatropha curcasin southern Haryana. The incidence of this disease has also been observed from other parts of Haryana too. Induction of systemic resistance in host plants through microbes and their bioactive metabolites are attaining popularity in modern agricultural practices. Studies on the plant growth-promoting rhizobacteria induced resistance in Jatropha curcas through phenyl propanoid metabolism against Rhizoctoniabataticola were undertaken at Chaudhary Charan Singh, Haryana Agricultural University, Regional Research Station, Bawal. Three plant growth-promoting rhizobacteria (PGPRs) viz., Pseudomonas maltophila, Pseudomonas fluorescens and Bacillus subtilis were evaluated for their potential to induce systemic resistance in Jatropha against root rot. The maximum increase of 97 per cent in total phenols, 120 per cent in peroxidase, 123 per cent in polyphenol oxidase, 101 per cent in phenylalanine ammonia lyase and 298 per cent in tyrosine ammonia lyase was detected in plants raised with Pseudomonas fluorescens+ Rhizoctoniaba-taticola inoculation in Jatropha curcas at 10 days post inoculation against control except total phenols where it was maximum (99%) at 30 DPI. There was slight or sharp decline in these parameters with age irrespective of inoculations. The pathogen challenged plants showed lower levels of total phenols and enzymes. The observations revealed that seed bacterization with Pseudomonas fluorescens results in accumulation of phenolics and battery of enzymes in response to pathogen infection and thereby induce resistance systemically.

scholarly journals The sss Colonization Gene of the Tomato-Fusarium oxysporum f. sp. radicis-lycopersici Biocontrol Strain Pseudomonas fluorescens WCS365 Can Improve Root Colonization of Other Wild-type Pseudomonas spp. Bacteria

2000 ◽  
Vol 13 (11) ◽  
pp. 1177-1183 ◽  
Author(s):  
Linda C. Dekkers ◽  
Ine H. M. Mulders ◽  
Claartje C. Phoelich ◽  
Thomas F. C. Chin-A-Woeng ◽  
André H. M. Wijfjes ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Pseudomonas Fluorescens ◽  
Pathogenic Fungus ◽  
Cell Types ◽  
Disease Suppression ◽  
Systemic Resistance ◽  
Root Tip ◽  
Wild Type ◽  
Tomato Root ◽  
Colonization Ability

We show that the disease tomato foot and root rot caused by the pathogenic fungus Fusarium oxysporum f. sp. radicis-lycopersici can be controlled by inoculation of seeds with cells of the efficient root colonizer Pseudomonas fluorescens WCS365, indicating that strain WCS365 is a bio-control strain. The mechanism for disease suppression most likely is induced systemic resistance. P. fluorescens strain WCS365 and P. chlororaphis strain PCL1391, which acts through the production of the antibiotic phenazine-1-carboxamide, were differentially labeled using genes encoding autofluorescent proteins. Inoculation of seeds with a 1:1 mixture of these strains showed that, at the upper part of the root, the two cell types were present as microcolonies of either one or both cell types. Microcolonies at the lower root part were predominantly of one cell type. Mixed inoculation tended to improve biocontrol in comparison with single inoculations. In contrast to what was observed previously for strain PCL1391, mutations in various colonization genes, including sss, did not consistently decrease the biocontrol ability of strain WCS365. Multiple copies of the sss colonization gene in WCS365 improved neither colonization nor biocontrol by this strain. However, introduction of the sss-containing DNA fragment into the poor colonizer P. fluorescens WCS307 and into the good colonizer P. fluorescens F113 increased the competitive tomato root tip colonization ability of the latter strains 16- to 40-fold and 8- to 16-fold, respectively. These results show that improvement of the colonization ability of wild-type Pseudomonas strains by genetic engineering is a realistic goal.

scholarly journals Induced Systemic Resistance in Arabidopsis thaliana Against Pseudomonas syringae pv. tomato by 2,4-Diacetylphloroglucinol-Producing Pseudomonas fluorescens

2012 ◽  
Vol 102 (4) ◽  
pp. 403-412 ◽  
Author(s):  
David M. Weller ◽  
Dmitri V. Mavrodi ◽  
Johan A. van Pelt ◽  
Corné M. J. Pieterse ◽  
Leendert C. van Loon ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Pseudomonas Fluorescens ◽  
Induced Resistance ◽  
Pseudomonas Syringae ◽  
Induced Systemic Resistance ◽  
Signal Transduction Pathway ◽  
Systemic Resistance ◽  
Wild Type ◽  
Challenge Inoculation ◽  
Dependent Signal

Pseudomonas fluorescens strains that produce the polyketide antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG) are among the most effective rhizobacteria that suppress root and crown rots, wilts, and damping-off diseases of a variety of crops, and they play a key role in the natural suppressiveness of some soils to certain soilborne pathogens. Root colonization by 2,4-DAPG-producing P. fluorescens strains Pf-5 (genotype A), Q2-87 (genotype B), Q8r1-96 (genotype D), and HT5-1 (genotype N) produced induced systemic resistance (ISR) in Arabidopsis thaliana accession Col-0 against bacterial speck caused by P. syringae pv. tomato. The ISR-eliciting activity of the four bacterial genotypes was similar, and all genotypes were equivalent in activity to the well-characterized strain P. fluorescens WCS417r. The 2,4-DAPG biosynthetic locus consists of the genes phlHGF and phlACBDE. phlD or phlBC mutants of Q2-87 (2,4-DAPG minus) were significantly reduced in ISR activity, and genetic complementation of the mutants restored ISR activity back to wild-type levels. A phlF regulatory mutant (overproducer of 2,4-DAPG) had ISR activity equivalent to the wild-type Q2-87. Introduction of DAPG into soil at concentrations of 10 to 250 μM 4 days before challenge inoculation induced resistance equivalent to or better than the bacteria. Strain Q2-87 induced resistance on transgenic NahG plants but not on npr1-1, jar1, and etr1 Arabidopsis mutants. These results indicate that the antibiotic 2,4-DAPG is a major determinant of ISR in 2,4-DAPG-producing P. fluorescens, that the genotype of the strain does not affect its ISR activity, and that the activity induced by these bacteria operates through the ethylene- and jasmonic acid-dependent signal transduction pathway.

Abiotic Compounds as Inducers of Resistance to Fusarium Wilt in Tomatoes

2018 ◽  
Vol 10 (9) ◽  
pp. 373
Author(s):  
Jose R. M. Campos Neto ◽  
Leonardo de J. M. G. de Oliveira ◽  
Nathalia B. Diniz ◽  
Ivaneide de O. Nascimento ◽  
Antonia Alice C. Rodrigues
Keyword(s):  
Polyphenol Oxidase ◽  
Fusarium Wilt ◽  
Mycelial Growth ◽  
Rating Scale ◽  
Systemic Resistance ◽  
Activity Levels ◽  
Neem Oil ◽  
In Vivo Experiments

To study the effects of resistance inducers used to control fusarium wilt in tomatoes due to the fungus Fusarium oxysporum Schlecht f. sp. lycopersici, we evaluated the effects of ASM (acibenzolar-S-methyl), Agro-Mos, chitosan, Biopirol and neem oil on F. oxysporum f. sp. lycopersici mycelial growth and sporulation and systemic resistance in tomatoes. In vitro experiments comprised evaluations of the products’ effects on the mycelial growth and sporulation of the PDA (potato dextrose agar) growth medium-cultured pathogen. In vivo experiments included product application to tomato plants of the Santa Cruz cultivar that were grown for 25 days on autoclaved soil, followed by determinations of disease severity and peroxidase, polyphenol oxidase and Beta-1,3-glucanase enzyme activity levels, which are related to the process of resistance induction. Pathogen inoculation occurred after 5 days at a concentration of 106 conidia/mL. We evaluated the disease according to a rating scale. Enzymatic activity was determined according to specific protocols. Neem oil controlled pathogen mycelial growth and sporulation, while ASM influenced sporulation. The products reduced the severity of wilt in the plants. We highlight neem oil, Agro-Mos and Biopirol due to their ability to induce significant peroxidase, polyphenol oxidase and Beta-1,3-glucanase expression, respectively.

scholarly journals Control of Fusarium Wilt of Radish by Combining Pseudomonas putida Strains that have Different Disease-Suppressive Mechanisms

2003 ◽  
Vol 93 (5) ◽  
pp. 626-632 ◽  
Author(s):  
Marjan de Boer ◽  
Peter Bom ◽  
Frodo Kindt ◽  
Joost J. B. Keurentjes ◽  
Ientse van der Sluis ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Fusarium Wilt ◽  
Plant Pathogens ◽  
Induced Systemic Resistance ◽  
Disease Suppression ◽  
Systemic Resistance ◽  
Single Application ◽  
Multiple Traits ◽  
Additional Mechanism ◽  
Single Strain

Biological control of soilborne plant pathogens in the field has given variable results. By combining specific strains of microorganisms, multiple traits antagonizing the pathogen can be combined and this may result in a higher level of protection. Pseudomonas putida WCS358 suppresses Fusarium wilt of radish by effectively competing for iron through the production of its pseudobactin siderophore. However, in some bioassays pseudobactin-negative mutants of WCS358 also suppressed disease to the same extent as WCS358, suggesting that an, as yet unknown, additional mechanism may be operative in this strain. P. putida strain RE8 induced systemic resistance against fusarium wilt. When WCS358 and RE8 were mixed through soil together, disease suppression was significantly enhanced to approximately 50% as compared to the 30% reduction for the single strain treatments. Moreover, when one strain failed to suppress disease in the single application, the combination still resulted in disease control. The enhanced disease suppression by the combination of P. putida strains WCS358 and RE8 is most likely the result of the combination of their different disease-suppressive mechanisms. These results demonstrate that combining biocontrol strains can lead to more effective, or at least, more reliable biocontrol of fusarium wilt of radish.

scholarly journals Induced Systemic Resistance in Arabidopsis thaliana in Response to Root Inoculation with Pseudomonas fluorescens CHA0

2003 ◽  
Vol 16 (10) ◽  
pp. 851-858 ◽  
Author(s):  
Annalisa Iavicoli ◽  
Emmanuel Boutet ◽  
Antony Buchala ◽  
Jean-Pierre Métraux
Keyword(s):  
Arabidopsis Thaliana ◽  
Jasmonic Acid ◽  
Pseudomonas Fluorescens ◽  
Transgenic Line ◽  
Induced Systemic Resistance ◽  
Antibiotic Activity ◽  
Systemic Resistance ◽  
Peronospora Parasitica ◽  
Molecular Processes ◽  
Molecular Determinants

Root inoculation of Arabidopsis thaliana ecotype Columbia with Pseudomonas fluorescens CHA0r partially protected leaves from the oomycete Peronospora parasitica. The molecular determinants of Pseudomonas fluorescens CHA0r for this induced systemic resistance (ISR) were investigated, using mutants derived from strain CHA0: CHA400 (pyoverdine deficient), CHA805 (exoprotease deficient), CHA77 (HCN deficient), CHA660 (pyoluteorin deficient), CHA631 (2,4-diacetylphloroglucinol [DAPG] deficient), and CHA89 (HCN, DAPG- and pyoluteorin deficient). Only mutations interfering with DAPG production led to a significant decrease in ISR to Peronospora parasitica. Thus, DAPG production in Pseudomonas fluorescens is required for the induction of ISR to Peronospora parasitica. DAPG is known for its antibiotic activity; however, our data indicate that one action of DAPG could be due to an effect on the physiology of the plant. DAPG at 10 to 100 μM applied to roots of Arabidopsis mimicked the ISR effect. CHA0r-mediated ISR was also tested in various Arabidopsis mutants and transgenic plants: NahG (transgenic line degrading salicylic acid [SA]), sid2-1 (nonproducing SA), npr1-1 (non-expressing NPR1 protein), jar1-1 (insensitive to jasmonic acid and methyl jasmonic acid), ein2-1 (insensitive to ethylene), etr1-1 (insensitive to ethylene), eir1-1 (insensitive to ethylene in roots), and pad2-1 (phytoalexin deficient). Only jar1-1, eir1-1, and npr1-1 mutants were unable to undergo ISR. Sensitivity to jasmonic acid and functional NPR1 and EIR1 proteins were required for full expression of CHA0r-mediated ISR. The requirements for ISR observed in this study in Peronospora parasitica induced by Pseudomonas fluorescens CHA0r only partially overlap with those published so far for Peronospora parasitica, indicating a great degree of flexibility in the molecular processes leading to ISR.

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