The expression profile of genes involved in osteoclastogenesis detected in whole blood of Arabian horses during 3 years of competing at race track

2019 ◽  
Vol 123 ◽  
pp. 59-64 ◽  
Author(s):  
Monika Stefaniuk-Szmukier ◽  
Katarzyna Ropka-Molik ◽  
Katarzyna Piórkowska ◽  
Monika Bugno-Poniewierska
Keyword(s):  
Expression Profile ◽  
Whole Blood ◽  
Race Track

scholarly journals Circular RNA expression profile in peripheral whole blood of lung adenocarcinoma by high

Medicine ◽  
2019 ◽  
Vol 98 (42) ◽  
pp. e17601 ◽  
Author(s):  
Yinyu Mu ◽  
Fuyi Xie ◽  
YunFei Huang ◽  
Dongdong Yang ◽  
Guodong Xu ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Expression Profile ◽  
Whole Blood ◽  
Circular Rna ◽  
Rna Expression

Gene Expression Profiling of Multiple Patients with Cyclic Thrombocytopenia Reveals Consistent Profile.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 219-219 ◽  
Author(s):  
Ruchira Sood ◽  
Erin Gourley ◽  
Stanley L. Schrier ◽  
Ronald Go ◽  
James L. Zehnder
Keyword(s):  
Gene Expression ◽  
Platelet Count ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Whole Blood ◽  
Gene Clusters ◽  
Underlying Disease ◽  
Multiple Time ◽  
Platelet Counts ◽  
History Of

Abstract Cyclic thrombocytopenia (CTP) is a rare disorder characterized by periodic changes in platelet count. While some previous reports suggest an association with several cytokines, the etiology of this disorder remains poorly characterized. Using DNA microarrays, we examined the gene expression profile in peripheral whole blood at multiple time points encompassing a cycle of platelet counts from two CTP patients. We hypothesized that the variation in gene expression program in whole blood would reflect on the transcriptional changes associated with or perhaps even underlying this disease. Genome-wide cDNA microarray analysis was performed using amplified RNA obtained from 11 and 8 whole blood samples from each patient. The first patient is a 41-year old male with a 2-year history of CTP while the second patient is a 54-year old male with a 3-year history of CTP. The period of both patients’ cycles is roughly 3 weeks. No associated underlying disease has been found in both patients. With a focus on 1500 genes that change 3 fold within each group of samples we observed clusters of gene expression in whole blood that correlate with changing platelet numbers in both patients. Significant variation in expression of a cluster of interferon responsive genes during the platelet count cycle was particularly striking in both samples. Interferon (IFN) therapy is known to suppress platelet counts, and this observation suggests that aberrant IFN levels and signalling could be in part responsible for CTP. At high platelet counts, platelet transcripts were detected in whole blood RNA as inferred by high expression of previously described platelet genes including TBXAS1, TUBB1, OAZ1, SEPT5, several mitochondrial genes, NRGN and F13A1. In addition, gene clusters including known genes as well as previously uncharacterized genes were found to correlate with the peak, increasing or decreasing trends of platelet counts. Briefly, GATA2 and NFE2 expression coincided with the platelet count peak, while Tyk2 and SOCS5 expression was consistent with a rising trend of platelet counts and GATA3 and JAK2 coincided with decreasing trend of platelet counts. These results show gene expression changes associated with CTP in all cell types in whole blood and pave the way for new investigation into regulation of platelet number in a rare and fascinating disease. Gene expression profile of whole blood of two CTP patients with platelet counts ranging from high to low and then increasing again from left to right of each panel Gene expression profile of whole blood of two CTP patients with platelet counts ranging from high to low and then increasing again from left to right of each panel

scholarly journals Comparative MicroRNA Expression Profiles of Cynomolgus Monkeys, Rat, and Human Reveal that miR-182 Is Involved in T2D Pathogenic Processes

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Jinghui Zhou ◽  
Yuhuan Meng ◽  
Shuai Tian ◽  
Junhui Chen ◽  
Mingyu Liu ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Expression Profile ◽  
Whole Blood ◽  
Copy Number ◽  
Target Genes ◽  
Expression Profiles ◽  
Tissue Expression ◽  
Mirna Expression Profile ◽  
Prevalent Disease ◽  
Suppression Of Gene Expression

Type 2 diabetes (T2D) is a prevalent disease that happens around the world and usually happens with insulin resistance. MicroRNAs (miRNAs) represented important roles in the suppression of gene expression and were proven to be related to human diseases. In this study, we used cynomolgus monkey fed with normal and high fatty diet (HFD), respectively, to analyze the miRNA expression profile in whole blood by deep sequencing. Finally in total 24 miRNAs with differential expression were filtered. Among them, miR-182 related to the insulin resistance by modulatingFOXO1and PI3K/AKT cascade and had the greatest copy number in the whole blood. Decrease of miR-182 in T2D cynomolgus individuals is completely consistent with the previous studies in human and rat. Integrating miR-182 tissue expression profile, target genes, and copy number in blood reveals that miR-182 plays a key role in crucial genes modulation, such asFOXO1andBHLHE22, which leads to potential hyperglycemia and modulates the insulin secretion. In addition, miR-182 might regulate the processes of both cell proliferation and apoptosis that play crucial role in determining the cells’ fate. Therefore, miR-182 can be a biomarker in diagnosis of the potential T2D that has benefits for medical purpose.

scholarly journals Whole Blood Gene Expression Profile Associated with Spontaneous Preterm Birth in Women with Threatened Preterm Labor

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e96901 ◽  
Author(s):  
Yujing Jan Heng ◽  
Craig Edward Pennell ◽  
Hon Nian Chua ◽  
Jonathan Edward Perkins ◽  
Stephen James Lye
Keyword(s):  
Gene Expression ◽  
Preterm Birth ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Whole Blood ◽  
Preterm Labor ◽  
Spontaneous Preterm Birth ◽  
Blood Gene Expression ◽  
Threatened Preterm Labor

scholarly journals Blood Genomic Expression Profile for Neuronal Injury

2003 ◽  
Vol 23 (3) ◽  
pp. 310-319 ◽  
Author(s):  
Yang Tang ◽  
Alex C. Nee ◽  
Aigang Lu ◽  
Ruiqiong Ran ◽  
Frank R. Sharp
Keyword(s):  
Gene Expression ◽  
Brain Injury ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Whole Blood ◽  
Neuronal Injury ◽  
Adult Rats ◽  
Mild Brain Injury ◽  
Microarray Analyses ◽  
Genomic Response

This study determined whether stroke and other types of insults produced a gene expression profile in blood that correlated with the presence of neuronal injury. Adult rats were subjected to ischemic stroke, intracerebral hemorrhage, status epilepticus, and insulin-induced hypoglycemia and compared with untouched, sham surgery, and hypoxia animals that had no brain injury. One day later, microarray analyses showed that 117 genes were upregulated and 80 genes were downregulated in mononuclear blood cells of the “injury” (n = 12) compared with the “no injury” (n = 9) animals. A second experiment examined the whole blood genomic response of adult rats after global ischemia and kainate seizures. Animals with no brain injury were compared with those with brain injury documented by TUNEL and PANT staining. One day later, microarray analyses showed that 37 genes were upregulated and 67 genes were downregulated in whole blood of the injury (n = 4) animals compared with the no-injury (n = 4) animals. Quantitative reverse transcription–polymerase chain reaction confirmed that the vesicular monoamine transporter-2 increased 2.3- and 1.6-fold in animals with severe and mild brain injury, respectively, compared with no-injury animals. Vascular tyrosine phosphatase-1 increased 2.0-fold after severe injury compared with no injury. The data support the hypothesis that there is a peripheral blood genomic response to neuronal injury, and that this blood response is associated with a specific blood mRNA gene expression profile that can be used as a marker of the neuronal damage.

scholarly journals The Blood and Muscle Expression Pattern of the Equine TCAP Gene during the Race Track Training of Arabian Horses

Animals ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 574 ◽  
Author(s):  
Monika Stefaniuk-Szmukier ◽  
Tomasz Szmatoła ◽  
Joanna Łątka ◽  
Bogusława Długosz ◽  
Katarzyna Ropka-Molik
Keyword(s):  
Skeletal Muscle ◽  
Whole Blood ◽  
Gluteus Medius ◽  
Transcript Abundance ◽  
Second Phase ◽  
Cross Sectional ◽  
Physical Effort ◽  
Muscle Plasticity ◽  
Race Track ◽  
Contractile Forces

Horse musculature has been shaped through evolution by environmental and human factors, which has resulted in several extraordinary adaptations to physical effort. Skeletal muscle plasticity results from the response to mechanical stimulation causing hypertrophy, where sarcomeres increase the muscle’s cross-sectional area under the influence of contractile forces. The aim of the present study was the evaluation of transcript abundance of the telethonin (TCAP) gene, which is a part of the sarcomere macromolecular mechanosensory complex in the gluteus medius muscle, and the whole blood of Arabian horses during flat race training. The analysis, performed by quantitative PCR, showed an increase of TCAP transcripts in skeletal muscle. However, in whole blood, the transcript abundance decreased after the first stage of training and further increased after the second phase. The obtained results indicate a lack of similarity of TCAP gene expression in both tissues.

scholarly journals Expression Pattern of Leptin and Its Receptors in Endometrioid Endometrial Cancer

2021 ◽  
Vol 10 (13) ◽  
pp. 2787
Author(s):  
Dariusz Boroń ◽  
Robert Nowakowski ◽  
Beniamin Oskar Grabarek ◽  
Nikola Zmarzły ◽  
Marcin Opławski
Keyword(s):  
Endometrial Cancer ◽  
Cancer Treatment ◽  
Expression Profile ◽  
Whole Blood ◽  
Leptin Receptor ◽  
Treatment Strategies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tissue Samples ◽  
Increased Risk ◽  
Risk Of Cancer

The identification of novel molecular markers and the development of cancer treatment strategies are very important as cancer incidence is still very high. Obesity can contribute to cancer progression, including endometrial cancer. Adipocytes secrete leptin, which, when at a high level, is associated with an increased risk of cancer. The aim of this study was to determine the expression profile of leptin-related genes in the endometrial tissue samples and whole blood of patients. The study material included tissue samples and whole blood collected from 30 patients with endometrial cancer and 30 without cancer. Microarrays were used to assess the expression profile of leptin-related genes. Then, the expression of leptin (LEP), leptin receptor (LEPR), leptin receptor overlapping transcript (LEPROT), and leptin receptor overlapping transcript-like 1 (LEPROTL1) was determined by the Real-Time Quantitative Reverse Transcription Reaction (RT-qPCR). The serum leptin concentration was evaluated using Enzyme-linked immunosorbent assay (ELISA). Leptin and its receptors were overexpressed both at the mRNA and protein levels. Furthermore, there were strong positive correlations between leptin levels and patient Body Mass Index (BMI). Elevated levels of leptin and its receptors may potentially contribute to the progression of endometrial cancer. These observations may be useful in designing endometrial cancer treatment strategies.

scholarly journals Integrating the analysis of transcriptome-wide association study and gene expression profile to identify genes associated with spondyloarthritis

2021 ◽  
Author(s):  
Jiawen Xu ◽  
Haibo Si ◽  
Yi Zeng ◽  
Yuangang Wu ◽  
Shaoyun Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Association Study ◽  
Candidate Genes ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Whole Blood ◽  
Genome Wide Association Study ◽  
Bone Diseases ◽  
Functional Enrichment

Abstract Background Spondyloarthritis(SpA) is a group of multi-factorial bone diseases influenced by genetic factors, environment and lifestyles. However, the genetic and pathogenic mechanism of SpA is still elusive. Methods Firstly, the tissue-specific transcriptome-wide association study (TWAS) of SpA was performed by utilizing the genome-wide association study (GWAS, including 3966 SpA patients and 452264 controls) summary data and gene expression weights of the whole blood and skeletal muscle. Secondly, the SpA-associated genes identified by TWAS were further compared with the differentially expressed genes(DEGs) detected by gene expression profile of SpA acquired from the Gene Expression Omnibus database (GEO, accession number:GSE58667). Finally, FUMA and Metascape tools were used to conduct gene functional enrichment and annotation analysis. Results TWAS detected 28 significant genes associated with SpA both in the whole blood and skeletal muscle, such as CTNNAL1 (PSM=0.0304, PWB=0.0096). Further comparing with gene expression profile of SpA, we identified 20 candidate genes which overlapped in TWAS, such as MCM4 (PTWAS=0.0132, PDEG=0.0275), KIAA1109 (PTWAS=0.0371,PDEG=0.0467). The enrichment analysis of the genes identified by TWAS detected 93 significant GO terms 33 and KEGG pathways, such as mitochondrion organization (GO:0007005, log10(P)= -4.29) and axon guidance(hsa04360, log10(P)= -4.26). Conclusion We identified multiple candidate genes genetically related to SpA. Our study may provide some novel clues for the further study of the genetic mechanism, diagnosis and treatment of SpA.

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