scholarly journals Antioxidant and antimicrobial activities of Spirulina platensis extracts and biogenic selenium nanoparticles against selected pathogenic bacteria and fungi

2021 ◽  
Author(s):  
Abdel-Moneim Eid Abdel-Moneim ◽  
Mohamed T. El-Saadony ◽  
Abdelrazeq M. Shehata ◽  
Ahmed M. Saad ◽  
Sami Ali Aldhumri ◽  
...  
Keyword(s):  
Spirulina Platensis ◽  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Selenium Nanoparticles ◽  
Bacteria And Fungi ◽  
Antioxidant And Antimicrobial Activities ◽  
Biogenic Selenium Nanoparticles

scholarly journals Evaluation of Vitamin Contents, Antioxidant and Antimicrobial Activities of Different Leaf Extracts of Taraxacum officinale (Dandelion)

2021 ◽  
pp. 13-26
Author(s):  
I. E. Daniel ◽  
K. N. Mathew ◽  
P. L. John
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Taraxacum Officinale ◽  
Antimicrobial Activities ◽  
Leaf Extracts ◽  
Phytochemical Compounds ◽  
Bacteria And Fungi ◽  
Antioxidant And Antimicrobial Activities

Methanol and ethyl acetate leaf extracts of Taraxacum officinale (dandelion) were evaluated for phytochemical compounds, vitamins, antioxidant and antimicrobial activities. Phytochemical compounds and vitamins were determined using standard procedures while antioxidant activity was determined using 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) and Ferric reducing/antioxidant power (FRAP) Assay.  Antimicrobial activity against various pathogenic bacteria and fungi were screened using disc diffusion method. The results indicated that the bioactive compounds (total phenol, flavonoids, saponins, tannins and alkaloids) determined quantitatively were present in appreciable concentration in both extracts. The result also showed that both extracts contain a variety of vitamins (A, B complex, C and E), with vitamins C and A having the highest concentration while the B-vitamins (B1, B2 and B3) and vitamin E were present in moderate concentrations. Both extracts showed significant scavenging and reducing ability comparable to the reference antioxidant, ascorbic acid in a dose dependent manner, with methanol exhibiting the highest scavenging and reducing capacity. The antimicrobial activity of both extracts showed appreciable broad spectrum activity against the pathogenic bacteria and fungi strains tested at various concentrations. Methanol extract was found to be most effective compared to ethyl acetate extract. These results indicated that the leaf extracts of dandelion possess antioxidant and antibacterial activity against the tested bacteria possibly due to the presence of bioactive compounds and other nutrients.

scholarly journals Antimicrobial Activities of Marine Sponge-Associated Bacteria

2021 ◽  
Vol 9 (1) ◽  
pp. 171
Author(s):  
Yitayal S. Anteneh ◽  
Qi Yang ◽  
Melissa H. Brown ◽  
Christopher M. M. Franco
Keyword(s):  
Pathogenic Bacteria ◽  
South Australia ◽  
Multidrug Resistant ◽  
Antimicrobial Activities ◽  
Marine Sponges ◽  
Bioactive Metabolites ◽  
Human Pathogens ◽  
Sponge Associated Bacteria ◽  
Microbial Symbionts ◽  
Bacteria And Fungi

The misuse and overuse of antibiotics have led to the emergence of multidrug-resistant microorganisms, which decreases the chance of treating those infected with existing antibiotics. This resistance calls for the search of new antimicrobials from prolific producers of novel natural products including marine sponges. Many of the novel active compounds reported from sponges have originated from their microbial symbionts. Therefore, this study aims to screen for bioactive metabolites from bacteria isolated from sponges. Twelve sponge samples were collected from South Australian marine environments and grown on seven isolation media under four incubation conditions; a total of 1234 bacterial isolates were obtained. Of these, 169 bacteria were tested in media optimized for production of antimicrobial metabolites and screened against eleven human pathogens. Seventy bacteria were found to be active against at least one test bacterial or fungal pathogen, while 37% of the tested bacteria showed activity against Staphylococcus aureus including methicillin-resistant strains and antifungal activity was produced by 21% the isolates. A potential novel active compound was purified possessing inhibitory activity against S. aureus. Using 16S rRNA, the strain was identified as Streptomyces sp. Our study highlights that the marine sponges of South Australia are a rich source of abundant and diverse bacteria producing metabolites with antimicrobial activities against human pathogenic bacteria and fungi.

scholarly journals Compositional Features of the “Kweli” Red Raspberry and Its Antioxidant and Antimicrobial Activities

Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1522
Author(s):  
Ana Luísa Vara ◽  
José Pinela ◽  
Maria Inês Dias ◽  
Jovana Petrović ◽  
António Nogueira ◽  
...  
Keyword(s):  
Nutritional Value ◽  
Unsaturated Fatty Acids ◽  
Thiobarbituric Acid ◽  
Antimicrobial Activities ◽  
Rubus Idaeus ◽  
Health Claims ◽  
Official Method ◽  
Red Raspberry ◽  
Bacteria And Fungi ◽  
Antioxidant And Antimicrobial Activities

Red raspberries (Rubus idaeus L.) are increasingly popular foods in contemporary diets due to their freshness, nutritional value and health claims. Among the existing cultivars, “Kweli” is one of the most productive and widely cultivated. In this study, the nutritional value and chemical composition of “Kweli” red raspberry were characterized by the official method of food analysis and chromatographic techniques, and its antioxidant and antimicrobial activities were tested against biological/biochemical oxidizable substrates and foodborne bacteria and fungi strains, respectively. Carbohydrates (including fructose and glucose, 14.3 and 12.6 g/100 g dw, respectively), proteins (6.8 g/100 g dw), and ashes (3.90 g/100 g dw) were major constituents. The fat content was quite low and constituted mainly by unsaturated fatty acids (58.3%), with a predominance of oleic acid. Fresh red raspberry also contained high levels of citric (2.7 g/100 g) and ascorbic (17 mg/100 g) acids. The anthocyanins (4.51 mg/g extract) cyanidin-O-hexoside and mostly cyanidin-O-sophoroside were identified in the red raspberry hydroethanolic extract, which was able to inhibit thiobarbituric acid reactive substances (TBARS) formation (EC50 of 122 µg/mL), oxidative hemolysis (IC50 of 298 µg/mL), and β-carotene bleaching (EC50 of 18.7 µg/mL). In turn, the extract was more effective than the food additive E224 against Bacillus cereus. All these results highlighted the nutritional quality of “Kweli” red raspberry and showed some compositional differences in relation to other cultivars. Therefore, its inclusion in a daily diet can be helpful to obtain nutrients and antioxidants and bring health benefits.

scholarly journals Isolation and Identification of Saccharomyces cerevisiae from Caterpillar Frass and their Probiotic Characterization

2019 ◽  
Vol 16 (1) ◽  
pp. 179-186 ◽  
Author(s):  
Ujwala Vinayak Khisti ◽  
Suyash Arun Kathade ◽  
Mayur Arjun Aswani ◽  
Pashmin Kaur Anand ◽  
Nirichan Kunchirman Bipinraj
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Yeast Cells ◽  
Isolation And Identification ◽  
Yeast Cultures ◽  
Isolation And Characterization ◽  
Wide Range ◽  
Probiotic Yeast ◽  
Bacteria And Fungi

Probiotics are live microorganisms which upon ingestion confer health benefits to the host and are widely applied for human and animal welfare. The present study reports the isolation of yeast cells from caterpillar frasses and its probiotic characterization. Out of four yeast cultures isolated, all found to be non-hemolytic and cultures designated as CV-I, CV-II CV-III and CV-IV showed good bile tolerance at 1.2%. These cultures possessed the ability to grow pH range of 1.5 – 10, exhibited auto-aggregation and co-aggregation capabilities, which are essential for growth in alimentary canal and reduction of pathogen adherence on the intestinal epithelial cells. All cultures exhibited good tolerance to temperature up to 42°C. Isolate CV-I showed wide range of antimicrobial activities against pathogenic bacteria and fungi. This study is the first report of isolation and characterization of probiotic yeast from caterpillar frass. The isolate CV-I has been identified as Saccharomyces cerevisiae by molecular methods. This culture is an ideal candidate for further probiotic exploration.

In-Vitro Cytotoxicity, Antimicrobial activities and DNA binding studies, of Novel Copper (II) complex with Heterocyclic base

2021 ◽  
pp. 3051-3055
Author(s):  
Shalini V. ◽  
Deepika R. ◽  
Arumugham M. N
Keyword(s):  
Dna Binding ◽  
Pathogenic Bacteria ◽  
Spectroscopic Method ◽  
Antimicrobial Activities ◽  
In Vitro Cytotoxicity ◽  
Binding Studies ◽  
Heterocyclic Base ◽  
Heterocyclic Ligand ◽  
Bacteria And Fungi

A novel ternary copper (II) complex have been synthesized by the addition of N, N-heterocyclic ligand with L-amino acid. The copper (II) complex, [Cu(1,10-phen) (L-Thr) Br] (where phen=1,10-phenanthroline and Threo=Threonine) characterized by various spectroscopic method. The intense UV band around 271nm was due to π-π* transition. The DNA binding study of these copper (II) complex are examined by UV–Visible, Emission spectroscopic, cyclic voltammetric and viscosity method. The results revealed that complex shown to be a intercalation mode of binding into DNA. The anticancer activity of Cu (II) complexes has capability to the kill HepG2 liver cancer cell as assessed by the MTT method, The Ic50value was found 21.50µg/ml The biological activity of the complex tested against certain pathogenic bacteria and fungi results revealed it was found to be potent antibacterial agent.

scholarly journals Antimicrobial activities of chicken β-defensin (4 and 10) peptides against pathogenic bacteria and fungi

2015 ◽  
Vol 5 ◽  
Author(s):  
Haitham A. Yacoub ◽  
Ahmed M. Elazzazy ◽  
Osama A. H. Abuzinadah ◽  
Ahmed M. Al-Hejin ◽  
Maged M. Mahmoud ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Bacteria And Fungi

scholarly journals Novel Synthetic Strategy of Cyclic Dithiocarbamates Catalyzed by Triton-B

2019 ◽  
Vol 31 (5) ◽  
pp. 1091-1094 ◽  
Author(s):  
R. Kishore ◽  
M. Kamboj ◽  
M. Shukla ◽  
N. Srivastava
Keyword(s):  
Pathogenic Bacteria ◽  
Phase Transfer ◽  
Antimicrobial Activities ◽  
Spectroscopic Methods ◽  
Mild Conditions ◽  
Synthetic Strategy ◽  
Green Approach ◽  
Bacteria And Fungi ◽  
Ethyl Bromopyruvate

A simple, rapid and green methodology to synthesize cyclic dithiocarbamates (compounds 1-9) was developed by the reaction of 1° amines, CS2 and ethyl 3-bromo-2-oxopropanoate (ethyl bromopyruvate) facilitated by Triton-B as phase transfer catalyst. These compounds (1-9) were characterized with the help of elemental analysis, IR, NMR and mass spectroscopic methods This efficient green approach requires mild conditions and gives good yield of product. In vitro antimicrobial activities of these compounds are reported against the pathogenic bacteria and fungi.

scholarly journals Antimicrobial activities of isolated probiotics and their metabolites against some pathogenic microorganisms

IIUC Studies ◽  
2018 ◽  
Vol 14 (1) ◽  
pp. 21-28
Author(s):  
Mohammed Abu Sayeed ◽  
Altaf Hossen ◽  
Repon Saha ◽  
Md Jakaria
Keyword(s):  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Antibacterial Property ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Maximum Effect ◽  
Zone Of Inhibition ◽  
Agar Media ◽  
Bacteria And Fungi

The study was aimed to finding the antimicrobial activities among probiotics isolated from different yoghurts and their metabolites against some common bacterial pathogens. The nutrient agar media overlay method (Disc diffusion Method) was used to determine the presence of antibacterial effects among the isolated probiotics. Probiotics produced potential antibacterial activities against several pathogenic bacteria and fungi. The maximum antibacterial property (13.5 mm of zone of inhibition) of bacterial strain found against Salmonella paratyphi. Conversly, bacterial metabolites produced maximum effect (10.3 mm of zone of inhibition) against Staphylocuccos aureaus. The antibacterial effect is one of the most important criteria for probiotics selection, and the verified antibacterial activities of the probiotics supports the development of these functional foods as a key to the enhancement of health in the consuming public.IIUC Studies Vol.14(1) June 2017: 21-28

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