Amelioration of Diethylnitrosamine (DEN) induced renal oxidative stress and inflammation by Carissa carandas embedded silver nanoparticles in rodents

Carissa carandas L. is traditionally used as antibacterial medicine and accumulates many antioxidant phytochemicals. Here, we expand this traditional usage with the green biosynthesis of silver nanoparticles (AgNPs) achieved using a Carissa carandas L. leaf extract as a reducing and capping agent. The green synthesis of AgNPs reaction was carried out using 1mM silver nitrate and leaf extract. The effect of temperature on the synthesis of AgNPs was examined using room temperature (25 °C) and 60 °C. The silver nanoparticles were formed in one hour by stirring at room temperature. In this case, a yellowish brown colour was developed. The successful formation of silver nanoparticles was confirmed by UV–Vis, Fourier transform infrared (FT-IR) and X-ray diffraction (XRD) analysis. The characteristic peaks of the UV-vis spectrum and XRD confirmed the synthesis of AgNPs. The biosynthesised AgNPs showed potential antioxidant activity through DPPH assay. These AgNPs also exhibited potential antibacterial activity against human pathogenic bacteria. The results were compared with the antioxidant and antibacterial activities of the plant extract, and clearly suggest that the green biosynthesized AgNPs can constitute an effective antioxidant and antibacterial agent.

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Pharmacological Properties of Biogenically Synthesized Silver Nanoparticles using Endophyte Bacillus cereus extract of Berberis lyceum against Oxidative Stress and Pathogenic Multidrug-Resistant Bacteria

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2021.07.009 ◽

2021 ◽

Author(s):

Neelam Mujaddidi ◽

Sobia Nisa ◽

Samha Al Ayoubi ◽

Yamin Bibi ◽

Salman Khan ◽

...

Keyword(s):

Oxidative Stress ◽

Silver Nanoparticles ◽

Bacillus Cereus ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Pharmacological Properties ◽

Multidrug Resistant Bacteria

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Oxidative Stress and Apoptotic Responses Elicited by Nostoc-Synthesized Silver Nanoparticles against Different Cancer Cell Lines

Cancers ◽

10.3390/cancers12082099 ◽

2020 ◽

Vol 12 (8) ◽

pp. 2099 ◽

Cited By ~ 3

Author(s):

Reham Samir Hamida ◽

Gadah Albasher ◽

Mashael Mohammed Bin-Meferij

Keyword(s):

Oxidative Stress ◽

Silver Nanoparticles ◽

Cancer Cells ◽

Hepg2 Cells ◽

Caspase 3 ◽

Mammalian Target Of Rapamycin ◽

B Cell Lymphoma ◽

Phosphorylated Akt ◽

Hct 116 ◽

Mcf 7

Green nanoparticles represent a revolution in bionanotechnology, providing opportunities to fight life-threatening diseases, such as cancer, with less risk to the environment and to human health. Here, for the first time, we systematically investigated the anticancer activity and possible mechanism of novel silver nanoparticles (N-SNPs) synthesized by Nostoc Bahar M against the MCF-7 breast cancer cells, HCT-116 colorectal adenocarcinoma cells, and HepG2 liver cancer cells, using cell viability assays, morphological characterization with inverted light and transmission electron microscopy, antioxidants and enzymes (glutathione peroxidase (GPx), glutathione (GSH), adenosine triphosphatase (ATPase), and lactate dehydrogenase (LDH)), and western blotting (protein kinase B (Akt), phosphorylated-Akt (p-Akt), mammalian target of rapamycin (mTOR), B-cell lymphoma 2 (Bcl-2), tumor suppressor (p53), and caspase 3). N-SNPs decreased the viability of MCF-7, HCT-116, and HepG2 cells, with half-maximal inhibitory concentrations of 54, 56, and 80 µg/mL, respectively. They also significantly increased LDH leakage, enhanced oxidative stress via effects on antioxidative markers, and caused metabolic stress by significantly decreasing ATPase levels. N-SNPs caused extensive ultrastructural alterations in cell and nuclear structures, as well as in various organelles. Furthermore, N-SNPs triggered apoptosis via the activation of caspase 3 and p53, and suppressed the mTOR signaling pathway via downregulating apoptosis-evading proteins in MCF-7, HCT-116, and HepG2 cells. Ultrastructural analysis, together with biochemical and molecular analyses, revealed that N-SNPs enhanced apoptosis via the induction of oxidative stress and/or through direct interactions with cellular structures in all tested cells. The cytotoxicity of Nostoc-mediated SNPs represents a new strategy for cancer treatment via targeting various cell death pathways. However, the potential of N-SNPs to be usable and biocompatible anticancer drug will depend on their toxicity against normal cells.

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Silver nanoparticles mitigate Aeromonas hydrophila-induced immune suppression, oxidative stress, and apoptotic and genotoxic effects in Oreochromis niloticus

Aquaculture ◽

10.1016/j.aquaculture.2021.736430 ◽

2021 ◽

Vol 535 ◽

pp. 736430

Author(s):

Walaa El-Houseiny ◽

Mohamed Fouad Mansour ◽

Wafaa A.M. Mohamed ◽

Naif A. Al-Gabri ◽

Ahmed A. El-Sayed ◽

...

Keyword(s):

Oxidative Stress ◽

Silver Nanoparticles ◽

Immune Suppression ◽

Aeromonas Hydrophila ◽

Oreochromis Niloticus ◽

Genotoxic Effects

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Cellular repair mechanisms triggered by exposure to silver nanoparticles and ionic silver in embryonic zebrafish cells

Environmental Science Nano ◽

10.1039/d1en00422k ◽

2021 ◽

Author(s):

Ana C. Quevedo ◽

Iseult Lynch ◽

Eugenia Valsami-Jones

Keyword(s):

Oxidative Stress ◽

Silver Nanoparticles ◽

Cell Death ◽

Zebrafish Embryo ◽

Genetic Damage ◽

Cellular Repair ◽

Embryo Cells ◽

Repair Mechanisms ◽

Toxicity Pathways ◽

Dynamic Interplay

The dynamic interplay between toxicity pathways (oxidative stress, calcium disturbances, genetic damage) caused by nanoparticles and the repair mechanisms of inhibition of cell division and induction of cell death is explored in zebrafish embryo cells.

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The oral administration of silver nanoparticles activates the kynurenine pathway in rat brain independently of oxidative stress

Chemico-Biological Interactions ◽

10.1016/j.cbi.2019.01.034 ◽

2019 ◽

Vol 302 ◽

pp. 22-27 ◽

Cited By ~ 4

Author(s):

Oluyomi Stephen Adeyemi ◽

Rhoda Ananu Uloko ◽

Oluwakemi Josephine Awakan ◽

Anne Adebukola Adeyanju ◽

David Adeiza Otohinoyi

Keyword(s):

Oxidative Stress ◽

Silver Nanoparticles ◽

Oral Administration ◽

Rat Brain ◽

Kynurenine Pathway

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Impact of silver nanoparticles exposure on neuro-behavior, hematology, and oxidative stress biomarkers of African catfish (Clarias gariepinus)

Aquaculture ◽

10.1016/j.aquaculture.2021.737082 ◽

2021 ◽

pp. 737082

Author(s):

Heba H. Mahboub ◽

Mariam H.E. Khedr ◽

Gehad E. Elshopakey ◽

Medhat S. Shakweer ◽

Dalia Ibrahim Mohamed ◽

...

Keyword(s):

Oxidative Stress ◽

Silver Nanoparticles ◽

Clarias Gariepinus ◽

African Catfish ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

And Oxidative Stress

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Role of Oxidative and Nitro-Oxidative Damage in Silver Nanoparticles Cytotoxic Effect against Human Pancreatic Ductal Adenocarcinoma Cells

Oxidative Medicine and Cellular Longevity ◽

10.1155/2018/8251961 ◽

2018 ◽

Vol 2018 ◽

pp. 1-15 ◽

Cited By ~ 17

Author(s):

Ewelina Barcińska ◽

Justyna Wierzbicka ◽

Agata Zauszkiewicz-Pawlak ◽

Dagmara Jacewicz ◽

Aleksandra Dabrowska ◽

...

Keyword(s):

Oxidative Stress ◽

Nitric Oxide ◽

Pancreatic Cancer ◽

Silver Nanoparticles ◽

Oxidative Damage ◽

Pancreatic Ductal Adenocarcinoma ◽

Cytotoxic Effect ◽

Mrna Level ◽

Ductal Adenocarcinoma ◽

Adenocarcinoma Cells

Pancreatic ductal adenocarcinoma is one of the most aggressive human malignancies, where the 5-year survival rate is less than 4% worldwide. Successful treatment of pancreatic cancer is a challenge for today’s oncology. Several studies showed that increased levels of oxidative stress may cause cancer cells damage and death. Therefore, we hypothesized that oxidative as well as nitro-oxidative stress is one of the mechanisms inducing pancreatic cancer programmed cell death. We decided to use silver nanoparticles (AgNPs) (2.6 and 18 nm) as a key factor triggering the reactive oxygen species (ROS) and reactive nitrogen species (RNS) in pancreatic ductal adenocarcinoma cells (PANC-1). Previously, we have found that AgNPs induced PANC-1 cells death. Furthermore, it is known that AgNPs may induce an accumulation of ROS and alteration of antioxidant systems in different type of tumors, and they are indicated as promising agents for cancer therapy. Then, the aim of our study was to evaluate the implication of oxidative and nitro-oxidative stress in this cytotoxic effect of AgNPs against PANC-1 cells. We determined AgNP-induced increase of ROS level in PANC-1 cells and pancreatic noncancer cell (hTERT-HPNE) for comparison purposes. We found that the increase was lower in noncancer cells. Reduction of mitochondrial membrane potential and changes in the cell cycle were also observed. Additionally, we determined the increase in RNS level: nitric oxide (NO) and nitric dioxide (NO2) in PANC-1 cells, together with increase in family of nitric oxide synthases (iNOS, eNOS, and nNOS) at protein and mRNA level. Disturbance of antioxidant enzymes: superoxide dismutase (SOD1, SOD2, and SOD3), glutathione peroxidase (GPX-4) and catalase (CAT) were proved at protein and mRNA level. Moreover, we showed cells ultrastructural changes, characteristic for oxidative damage. Summarizing, oxidative and nitro-oxidative stress and mitochondrial disruption are implicated in AgNPs-mediated death in human pancreatic ductal adenocarcinoma cells.

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