Pre-eclampsia and eclampsia associated with increased lipid peroxidation and decreased serum vitamin E levels

This study was carried out in 140 healthy subjects who were divided into three subgroups of age: young (21–40 years), mature (41–60 years), and elderly ( 61–85 years) to investigate lipid peroxides and the antioxidant system in serum and low-density lipoproteins (LDL). Serum levels of cholesterol and LDL-cholesterol increased with age. The elderly group was found to have higher polyunsaturated fatty acid (PUFA) levels, thiobarbituric acid reactive substances (TBARS), diene conjugates, and lower cholesterol-adjusted vitamin E levels and antioxidant activity (AOA) as compared to the young group. No age-related difference was detected in serum vitamin C levels. Age correlated positively with serum cholesterol, LDL-cholesterol, PUFA, TBARS, diene conjugates, and negatively with cholesterol-adjusted vitamin E levels and AOA. In addition, endogenous LDL diene conjugate levels and the susceptibility of LDL to copper-induced lipid peroxidation increased in elderly subjects as compared with young subjects. In addition, positive correlations were detected between age and LDL endogenous diene conjugate levels and TBARS formation after copper incubation. However, the susceptibility of whole serum to copper-induced lipid peroxidation did not change in young and elderly subjects. Our results show that endogenous lipid peroxide levels in serum and LDL, and the susceptibility of LDL to copper-induced oxidation, increased with aging in humans.

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Lipid Peroxidation and Antioxidant Status in Preeclampsia

Journal of Enam Medical College ◽

10.3329/jemc.v1i2.11462 ◽

2012 ◽

Vol 1 (2) ◽

pp. 56-59 ◽

Cited By ~ 1

Author(s):

Rokeya Begum

Keyword(s):

Lipid Peroxidation ◽

Vitamin E ◽

Pregnant Women ◽

Serum Vitamin ◽

Normal Pregnancy ◽

Cellular Damage ◽

Control Group ◽

Medical Complication ◽

Lipid Peroxidation Product ◽

Peroxidation Product

Background: Preeclampsia is the most common and major medical complication of pregnancy with a high incidence of maternal and foetal morbidity and mortality. During pregnancy abnormally increased lipid peroxidation and free radical formation as well as significantly decreased antioxidants production in maternal blood may lead to pathogenesis of preeclampsia. So, we designed this study as little information is known about lipid peroxidation and antioxidant level in preeclampsia.Objectives: To assess the serum malondialdehyde (MDA) level as a lipid peroxidation product and vitamin E (antioxidant) level in women with preeclampsia as well as in normal pregnancy and to compare the values.Materials and Methods: The study was conducted on 60 women aged from 25 to 35 years in the department of Biochemistry, Budi Kemuliaan Maternity Hospital (BKMH) in Jakarta during the period April to July 2004. Twenty were normal pregnant women and 20 were preeclamptic patients. For comparison age matched 20 apparently healthy nonpregnant women were included in the study. The study subjects were selected from outpatient department (OPD) of Obstetrics and Gynaecology of BKMH in Jakarta. Serum MDA (lipid peroxidation product) level was measured by thiobarbituric acid reactive substances assay (TBRAS) method and vitamin E was estimated spectroflurometrically. Data were analyzed by unpaired Students t test between the groups by using SPSS version 12.Results: The mean serum MDA levels were significantly higher in normal pregnancy and also in preeclampsia than that of nonpregnant control group women (p<0.001). Again the serum MDA levels were significantly higher in preeclampsia than that of normal pregnant women (p<0.001). The serum vitamin E levels were significantly lower in preeclampsia and also in normal pregnancy than that of nonpregnant control women (p<0.001). Moreover, the serum vitamin E levels were significantly lower in preeclampsia compared to that of normal pregnancy which was also statistically significant (p<0.001)Conclusion: As in preeclamptic group level of oxidation product is high and antioxidant level is low, it can be assumed that in preeclampsia, serum antioxidants are excessively utilized to counteract the cellular damage mediated by free radicals production and deficient antioxidants as well as increased lipid peroxidations may be the important factors in the pathogenesis of preeclampsia. DOI: http://dx.doi.org/10.3329/jemc.v1i2.11462 J Enam Med Col 2011; 1(2): 56-59

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Dietary omega-3 fatty acid supplementation does not impair vitamin E status or promote lipid peroxidation in growing horses

Journal of Animal Science ◽

10.1093/jas/skab177 ◽

2021 ◽

Author(s):

Sarah H White-Springer ◽

Kelly R Vineyard ◽

Jan Kivipelto ◽

Lori K Warren

Keyword(s):

Lipid Peroxidation ◽

Body Weight ◽

Vitamin E ◽

Repeated Measures ◽

Antioxidant Status ◽

Serum Vitamin ◽

Omega 3 ◽

Potential Health ◽

Increased Risk ◽

Vitamin E Status

Abstract Omega-3 (n-3; ω-3) fatty acids (FA) are often included in the diet for their potential health benefits. However, because oxidative potential is increased with the degree of unsaturation in vitro, polyunsaturated FA such as eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) may be at increased risk of lipid peroxidation. We aimed to determine the effects of dietary n-3 FA supplementation on antioxidant status and lipid peroxidation in yearling horses. Quarter Horses (mean ± SEM; 14.6 ± 0.2 mo) were randomly assigned to receive no n-3 FA supplementation (CON; n = 6) or 60 mg n-3/kg body weight from milled flaxseed (FLAX; n = 6) or encapsulated fish oil (FISH; n = 6). All horses received a basal diet of mixed grain concentrate fed individually at 1.5% body weight (dry matter basis) and ad libitum bahiagrass pasture forage. Blood samples were obtained before and after 70 d of supplementation to evaluate vitamin E, selenium, lipids, antioxidant status, and oxidative stress. Data were analyzed using a mixed model ANOVA with repeated measures. Supplementation with n-3 FA did not reduce serum vitamin E or Se and, in fact, elevated (P ≤ 0.0003) vitamin E status in FISH horses. At d 70, serum triglycerides were lower in FISH and FLAX horses than CON horses (P ≤ 0.02) and F2-isoprostanes were lower in FISH than CON horses (P = 0.0002). Dietary n-3 FA had no effect on cholesterol, reduced and oxidized glutathione, glutathione peroxidase, and thiobarbituric acid-reactive substances. In growing horses fed to meet their vitamin E requirements, supplementation with 60 mg n-3/kg body weight did not negatively affect vitamin E status or promote lipid peroxidation. Elevated vitamin E status in horses fed FISH, coupled with lower serum F2-isoprostanes, further suggest that the longer-chain, highly unsaturated n-3 FA, EPA and DHA, may actually attenuate lipid peroxidation.

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Protective role of supplemental vitamin E on lipid peroxidation, vitamins E, A and some mineral concentrations of broilers reared under heat stress

Veterinární Medicína ◽

10.17221/7870-vetmed ◽

2001 ◽

Vol 46 (No. 5) ◽

pp. 140-144 ◽

Cited By ~ 41

Author(s):

K. Sahin ◽

N. Sahin ◽

M. Onderci ◽

S. Yaralioglu ◽

O. Kucuk

Keyword(s):

Lipid Peroxidation ◽

Heat Stress ◽

Vitamin E ◽

Management Practice ◽

Serum Vitamin ◽

Basal Diet ◽

Dietary Vitamin ◽

Broiler Chicks ◽

Supplemental Vitamin ◽

Vitamin E Supplementation

An experiment utilizing Cobb-500 male broilers was conducted to evaluate the effects of vitamin E supplementation at various concentrations on malonyldialdehyde (MDA) as an indicator of lipid peroxidation, serum and liver concentrations of antioxidant vitamins and some minerals of broilers reared under heat stress (32°C). One day-old 150 male broilers were randomly assigned to 5 treatment groups, 3 replicates of 10 birds each. The birds received either a basal diet or basal diet supplemented with vitamin E (dl-a-tocopherol acetate) at 62.5, 125, 250, or 500 mg/kg of diet. Increased supplemental vitamin E linearly increased serum vitamin E and A, but decreased (P = 0.001) MDA concentrations. Increasing dietary vitamin E supplementation also resulted in linear increases in liver vitamin E and A concentrations, but linear decreases in MDA concentrations (P = 0.01). Increasing dietary vitamin E caused a linear increase in serum concentrations of Fe and Zn (P= 0.001), but a decrease in serum concentration of Cu (P = 0.001). Results of the present study conclude that in broiler chicks reared under heat stress a 250 mg of vitamin E supplementation can be considered as a protective management practice in a broiler diet, reducing the negative effects of heat stress.

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Smoking habits and plasma lipid peroxide and vitamin E levels in never-treated first-episode patients with schizophrenia

The British Journal of Psychiatry ◽

10.1192/bjp.176.3.290 ◽

2000 ◽

Vol 176 (3) ◽

pp. 290-293 ◽

Cited By ~ 32

Keyword(s):

Body Mass Index ◽

Lipid Peroxidation ◽

Vitamin E ◽

Body Mass ◽

Smoking Status ◽

Serum Vitamin ◽

Plasma Lipid ◽

Lipid Peroxide ◽

First Episode ◽

Plasma Lipid Peroxide

BackgroundLipid peroxidation may be increased in schizophrenia, due to the illness, lifestyle or medication.AimsTo determine plasma lipid peroxide levels and serum vitamin E and A levels in first-episode never-treated people with schizophrenia and in controls.MethodThirty in-patients with a first episode of schizophrenia or schizophreniform psychosis were recruited, as were controls matched for gender, age, smoking and dietary status. Blood samples were taken, smoking status was recorded and body mass index measured.ResultsThere were no significant differences between patients and controls in plasma lipid peroxide levels. Seventy-three per cent of the patients smoked. Patients who smoked had a higher mean lipid peroxide level than non-smokers. Seventy-seven per cent of patients and 70% of controls had a ratio of vitamin Eto cholesterol of less than 5. Body mass index was lower in patients than in controls.ConclusionsAs a result of the high prevalence of smoking this group shows increased lipid peroxidation. Low serum ratios of vitamin Eto cholesterol in both patients and controls suggest an unsatisfactory diet.

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282 Impact of dietary peroxidized lipids and antioxidant supplementation in feed on growth performance and oxidative status of newly weaned piglets

Journal of Animal Science ◽

10.1093/jas/skz258.243 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 117-118

Author(s):

Ysenia Victoria Silva-Guillen ◽

Gabriela Martinez ◽

Consuelo Arellano ◽

R Dean Boyd ◽

Eric van Heugten

Keyword(s):

Lipid Peroxidation ◽

Vitamin E ◽

Soybean Oil ◽

Growth Performance ◽

Serum Vitamin ◽

Oxidative Status ◽

Dietary Vitamin ◽

Weaned Piglets ◽

Antioxidant Supplementation ◽

Tocopherol Acetate

Abstract The objective of this study was to evaluate the use of dietary vitamin E (VitE) and polyphenols on growth and oxidative status of weaned piglets fed peroxidized lipids. Pigs (n = 192; 21-d of age; 6.62±1.04 kg BW) were assigned within sex and weight blocks to a 2x3 factorial arrangement using 48 pens (4 pigs/pen; 8 replications/treatment). Factors consisted of lipid peroxidation (6% human-grade soybean oil or 6% peroxidized soybean oil), and antioxidant supplementation (control diet containing 25 IU/kg dl-α-tocopherol-acetate; control with 200 IU/kg additional dl-α-tocopherol-acetate; or control with 400 mg/kg polyphenols). Peroxidation was accomplished by heating control oil at 80°C with constant air flow at 50 L/min for 12 d (initial peroxide, anisidine value, hexanal, and 2,4-decadienal concentrations were 4.1 vs. 141.6 meq/kg, 1.7 vs. 106, < 5 vs. 99 mg/kg, and 8 vs. 720 mg/kg for control and peroxidized oil, respectively). Diets were fed in 2 phases (14 and 21 days). Overall, peroxidized lipids decreased (P < 0.001) BW (23.16 vs. 18.74 kg), ADG (473 vs. 346 g/day), ADFI (658 vs. 535 g/day) and G:F (719 vs. 647 g/kg). Lipid peroxidation decreased serum vitamin E (P < 0.001) and this decrease was larger on d35 (1.82 vs. 0.81 mg/kg) than d14 (1.95 vs. 1.38 mg/kg). Supplemental VitE, but not polyphenols, increased (P < 0.002) serum vitamin E by 84 and 22%, for control and peroxidized diets, respectively (interaction, P = 0.001). Serum malondialdehyde decreased (P < 0.001) with peroxidation on d14, but not d35 and protein carbonyl increased (P < 0.001) with peroxidation on d35, but not d14. Serum 8-hydroxydeoxyguanosine was not affected (P > 0.05). Total antioxidant capacity decreased with peroxidation (P < 0.001) and increased with VitE (P = 0.065) and polyphenols (P = 0.046) in the control oil diet only. Peroxidized lipids reduced growth performance, which could not be corrected with supplemental vitamin E or polyphenols in the diet.

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