CYTOCHEMICAL AND CYTOGENETIC FINDINGS IN A CASE OF CHRONIC NEUTROPHILIC LEUKÆMIA OF MATURE CELL TYPE

The Lancet ◽  
1964 ◽  
Vol 283 (7329) ◽  
pp. 387-388 ◽  
Author(s):  
J. Tanzer ◽  
P. Harel ◽  
M. Boiron ◽  
Jean Bernard
Keyword(s):  
Cell Type ◽  
Mature Cell ◽  
Mature Cell Type

Multipotential stem cells in adult mouse gastric epithelium

2002 ◽  
Vol 283 (3) ◽  
pp. G767-G777 ◽  
Author(s):  
Matthew Bjerknes ◽  
Hazel Cheng
Keyword(s):  
Stem Cells ◽  
Adult Mouse ◽  
Cell Lineage ◽  
Cell Types ◽  
Lineage Tracing ◽  
Gastric Epithelium ◽  
Chemical Mutagenesis ◽  
Cell Type ◽  
Mature Cell ◽  
Mature Cell Type

Previous studies of chimeric animals demonstrate that multipotential stem cells play a role in the development of the gastric epithelium; however, despite much effort, it is not clear whether they persist into adulthood. Here, chemical mutagenesis was used to label random epithelial cells by loss of transgene function in adult hemizygous ROSA26 mice, a mouse strain expressing the transgene lacZ in all tissues. Many clones derived from such cells contained all the major epithelial cell types, thereby demonstrating existence of functional multipotential stem cells in adult mouse gastric epithelium. We also observed clones containing only a single mature cell type, indicating the presence of long-lived committed progenitors in the gastric epithelium. Similar results were obtained in duodenum and colon, showing that this mouse model is suitable for lineage tracing in all regions of the gastrointestinal tract and likely useful for cell lineage studies in other adult renewing tissues.

CYTOCHEMICAL AND CYTOGENETIC FINDINGS IN CHRONIC NEUTROPHILIC LEUKÆMIA OF MATURE CELL TYPE

The Lancet ◽  
1964 ◽  
Vol 284 (7369) ◽  
pp. 1123 ◽  
Author(s):  
N Gingold ◽  
Carmen Dulgheru Oproiu ◽  
Natalia Comanescu
Keyword(s):  
Cell Type ◽  
Mature Cell ◽  
Mature Cell Type

scholarly journals Histone acetylation in chicken erythrocytes. Rates of acetylation and evidence that histones in both active and potentially active chromatin are rapidly modified

1988 ◽  
Vol 250 (1) ◽  
pp. 233-240 ◽  
Author(s):  
D E Zhang ◽  
D A Nelson
Keyword(s):  
Beta Globin ◽  
Cell Type ◽  
Mature Cell ◽  
Active Chromatin ◽  
Rate Analysis ◽  
Histone Lysine ◽  
Quantitative Results ◽  
Chicken Erythrocytes ◽  
Immature Cells ◽  
Rule Out

Of the modifiable histone lysine sites, 2-4% participate in dynamic acetylation in chicken erythrocytes, suggesting the involvement of no more than 1-2% of the total genome. The rates and chromatin locality of this dynamic acetylation were studied in both chicken mature and immature red blood cells. In mature erythrocytes, two rates of acetylation of radiolabelled, monoacetylated H4 are observed, with half-lives of approximately 12 and approximately 300 min. In contrast, only one rate with a half-life (t1/2) of 12 min is observed in immature cells, and further experiments rule out the possibility of a slow rate of acetylation (with a t1/2 of approximately 300 min) for any form of H4 in this cell type. The simplest interpretation of these quantitative results, taken together with the behaviour of H3, H2B and H4 observed on the fluorograms used for rate analysis, is that a portion of the rapidly acetylated histone is converted to a more slowly acetylated form during erythrocyte maturation. The transcriptionally active adult beta-globin and H5 nucleohistone, which are presumably converted to potentially active chromatin during the maturation process, remain of the rapidly acetylated form in the mature cell.

scholarly journals Implications of DPP4 modification of proteins that regulate stem/progenitor and more mature cell types

Blood ◽  
2013 ◽  
Vol 122 (2) ◽  
pp. 161-169 ◽  
Author(s):  
Xuan Ou ◽  
Heather A. O’Leary ◽  
Hal E. Broxmeyer
Keyword(s):  
Blood Cells ◽  
Progenitor Cell ◽  
Potential Role ◽  
Cell Function ◽  
Cell Types ◽  
Regulatory Proteins ◽  
Cell Type ◽  
Mature Cell ◽  
Hematopoietic Stem

Abstract Dipeptidylpeptidase (DPP) 4 has the potential to truncate proteins with a penultimate alanine, proline, or other selective amino acids at the N-terminus. DPP4 truncation of certain chemokines, colony-stimulating factors, and interleukins have recently been linked to regulation of hematopoietic stem/progenitor cells, more mature blood cells, and other cell types. We believe that the potential role of DPP4 in modification of many regulatory proteins, and their subsequent effects on numerous stem/progenitor and other cell-type functions has not been adequately appreciated. This review addresses the potential implications of the modifying effects of DPP4 on a large number of cytokines and other growth-regulating factors with either proven or putative DPP4 truncation sites on hematopoietic cells, and subsequent effects of DPP4-truncated proteins on multiple aspects of steady-state and stressed hematopoiesis, including stem/progenitor cell, and more mature cell, function.

Leukemia Cell Differentiation upon Targeted Therapy Revealed By a Systems Biology Approach

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 5202-5202
Author(s):  
Yulin Li ◽  
Jun Seita ◽  
Dean W Felsher ◽  
David Dill
Keyword(s):  
Gene Expression ◽  
Systems Biology ◽  
Expression Profiles ◽  
Hematopoietic Cells ◽  
Leukemia Cell ◽  
Gene Expression Profiles ◽  
Human Leukemia ◽  
Cell Type ◽  
Mature Cell ◽  
Hematopoietic Stem

Abstract Accurately determining the differentiation status of hematopoietic cells is crucial for understanding tumorigenesis and developing novel therapies for human leukemia. However, most current methods are subject to various biases. In this paper, we have developed a novel systems biology method, called the Lineage Maturation Index, to define the changes in differentiation state of hematopoietic cells based on comprehensive gene expression profiles. The changes in gene expression profiles of a specific hematopoietic lineage are shown asthe “lineage vector” from a point representing the gene expression of a hematopoietic stem cell (HSC) to another point representing a mature cell. By projecting the gene expression profile of a specific hematopoietic cell type onto the “lineage vector”, we can compute the LMI and define the relative differentiation state of this cell type. A shift in LMI from HSCs towards mature cell types indicates differentiation. We have confirmed the LMI method can track the differentiation and transdifferentiation of normal hematopoietic cells. Applying this method, we have shown that differentiation is a major consequence of various targeted therapies against leukemia irrespective of the driving oncogenes. Furthermore, we have used the method to screen a drug response library and discovered several drugs currently in clinical use for other purposes have potent differentiation activities in leukemia cells. Therefore, the LMI method is a robust computational approach to characterize differentiation of the hematopoietic system and discover novel differentiation therapy for leukemia. Disclosures No relevant conflicts of interest to declare.

scholarly journals Induced pluripotent stem cell–derived human platelets: one step closer to the clinic

2010 ◽  
Vol 207 (13) ◽  
pp. 2781-2784 ◽  
Author(s):  
Christos Gekas ◽  
Thomas Graf
Keyword(s):  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Cell Types ◽  
Ips Cells ◽  
Human Platelets ◽  
Cell Type ◽  
Mature Cell ◽  
High Demand ◽  
One Step ◽  
Induced Pluripotent

The era of induced pluripotent stem (iPS) cells carries with it the promise of virtually unlimited sources of autologous cells for regenerative medicine. However, efficiently differentiating iPS cells into fully functional mature cell types remains challenging. A new study reporting the formation of fully functional platelets from human iPS (hiPS) cells improves upon recent efforts to generate this enucleated cell type, which remains in high demand for therapeutic transfusions. Notably, their lack of nucleus renders platelets unable to retain the pluripotent or tumorigenic properties of iPS cells.

Ultrastructural Correlations between Clonal Human Tumor Colonies and in Vivo Neoplasms

1982 ◽  
Vol 40 ◽  
pp. 210-211
Author(s):  
M.J. Murphy ◽  
R.R. Price ◽  
J.C. Sloman
Keyword(s):  
Cultured Cells ◽  
Human Tumor ◽  
Cell Type ◽  
Tumor Mass ◽  
Initial Cell ◽  
Cloning Assay ◽  
Human Tumor Cloning ◽  
The Relationship

The in vitro human tumor cloning assay originally described by Salmon and Hamburger has been applied recently to the investigation of differential anti-tumor drug sensitivities over a broad range of human neoplasms. A major problem in the acceptance of this technique has been the question of the relationship between the cultured cells and the original patient tumor, i.e., whether the colonies that develop derive from the neoplasm or from some other cell type within the initial cell population. A study of the ultrastructural morphology of the cultured cells vs. patient tumor has therefore been undertaken to resolve this question. Direct correlation was assured by division of a common tumor mass at surgical resection, one biopsy being fixed for TEM studies, the second being rapidly transported to the laboratory for culture.

Some Observations on Changes in Denervated Taste Buds of Circumvallate Papillae of Rabbits

1969 ◽  
Vol 27 ◽  
pp. 308-309
Author(s):  
Sunao Fujimoto ◽  
Raymond G. Murray ◽  
Assia Murray
Keyword(s):  
Taste Buds ◽  
Taste Bud ◽  
Cell Type ◽  
Dark Cells ◽  
Type Iii ◽  
Circumvallate Papillae ◽  
Taste Bud Cells ◽  
Light Cells

Taste bud cells in circumvallate papillae of rabbit have been classified into three groups: dark cells; light cells; and type III cells. Unilateral section of the 9th nerve distal to the petrosal ganglion was performed in 18 animals, and changes of each cell type in the denervated buds were observed from 6 hours to 10 days after the operation.Degeneration of nerves is evident at 12 hours (Fig. 1) and by 2 days, nerves are completely lacking in the buds. Invasion by leucocytes into the buds is remarkable from 6 to 12 hours but then decreases. Their extrusion through the pore is seen. Shrinkage and disturbance in arrangement of cells in the buds can be seen at 2 days. Degenerated buds consisting of a few irregular cells and remnants of degenerated cells are present at 4 days, but buds apparently normal except for the loss of nerve elements are still present at 6 days.

Dendritic cells of the human epidermis: immuno-electron microscopic studies of la antigen reactivity

1978 ◽  
Vol 36 (2) ◽  
pp. 644-645
Author(s):  
G. Rowden ◽  
M. G. Lewis ◽  
T. M. Phillips
Keyword(s):  
Dendritic Cells ◽  
Langerhans Cells ◽  
Cell Types ◽  
Cell Type ◽  
Electron Microscopic ◽  
La Antigen ◽  
Microscopic Studies ◽  
A Cell ◽  
C3 Receptors ◽  
Antigen Reactivity

Langerhans cells of mammalian stratified squamous epithelial have proven to be an enigma since their discovery in 1868. These dendritic suprabasal cells have been considered as related to melanocytes either as effete cells, or as post divisional products. Although grafting experiments seemed to demonstrate the independence of the cell types, much confusion still exists. The presence in the epidermis of a cell type with morphological features seemingly shared by melanocytes and Langerhans cells has been especially troublesome. This so called "indeterminate", or " -dendritic cell" lacks both Langerhans cells granules and melanosomes, yet it is clearly not a keratinocyte. Suggestions have been made that it is related to either Langerhans cells or melanocyte. Recent studies have unequivocally demonstrated that Langerhans cells are independent cells with immune function. They display Fc and C3 receptors on their surface as well as la (immune region associated) antigens.

Lymphoproliferative disorders (LPD) involving lungs: T and B cell subsets within pulmonary infiltrates and in peripheral blood

1984 ◽  
Vol 42 ◽  
pp. 700-701
Author(s):  
Irene Stachura ◽  
Milton H. Dalbow ◽  
Michael J. Niemiec ◽  
Matias Pardo ◽  
Gurmukh Singh ◽  
...  
Keyword(s):  
B Cells ◽  
B Cell ◽  
Lymphoproliferative Disorders ◽  
Mixed Population ◽  
Lymphoid Cells ◽  
Lymphomatoid Granulomatosis ◽  
Cell Type ◽  
Cell Surface Antigens ◽  
Pulmonary Infiltrates ◽  
B Cell Subsets

Lymphoid cells were analyzed within pulmonary infiltrates of six patients with lymphoproliferative disorders involving lungs by immunofluorescence and immunoperoxidase techniques utilizing monoclonal antibodies to cell surface antigens T11 (total T), T4 (inducer/helper T), T8 (cytotoxic/suppressor T) and B1 (B cells) and the antisera against heavy (G,A,M) and light (kappa, lambda) immunoglobulin chains. Three patients had pseudolymphoma, two patients had lymphoma and one patient had lymphomatoid granulomatosis.A mixed population of cells was present in tissue infiltrates from the three patients with pseudolymphoma, IgM-kappa producing cells constituted the main B cell type in one patient. In two patients with lymphoma pattern the infiltrates were composed exclusively of T4+ cells and IgG-lambda B cells predominated slightly in the patient with lymphomatoid granulomatosis.

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