Polyunsaturated fatty acids in the blood of spontaneously or induced muricidal male Wistar rats

The consumption of prebiotics is known to affect the balance of gut microbiota. The purpose of this study was to explore how a galactomannan-rich effervescent drink can affect the population of Lactobacillus, Bifidobacterium, E. coli, and the concentration of short-chain fatty acids in the cecum of rats. Twenty-eight male Wistar rats (aged 2 months) were divided equally into 7 groups and treated orally each day for 15 days with 2 mL effervescent drinks with increasing levels of prebiotic galactomannan. The dosage of 500 mg galactomannan increased the growth of Lactobacillus spp. and Bifidobacterium spp. with inhibition of the growth of E.coli with increased formation of short-chain fatty acids such as acetate, propionate, and butyrate in the cecum of rats.

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Modulation of Systemic and Aortic Nitric Oxide by Melatonin and n-3 Polyunsaturated Fatty Acids in Isoproterenol Affected Spontaneously Hypertensive and Normotensive Wistar Rats

Physiological Research ◽

10.33549/physiolres.933400 ◽

2016 ◽

pp. S109-S118 ◽

Cited By ~ 1

Author(s):

K. K. CHAUDAGAR ◽

C. VICZENCZOVA ◽

B. SZEIFFOVA BACOVA ◽

T. EGAN BENOVA ◽

M. BARANCIK ◽

...

Keyword(s):

Nitric Oxide ◽

Fatty Acids ◽

Polyunsaturated Fatty Acids ◽

Wistar Rats ◽

Dependent Manner ◽

Pufa Supplementation ◽

Pufa Intake ◽

Spontaneously Hypertensive ◽

No Release ◽

Strain Dependent

We aimed to explore the effects of melatonin and n-3 polyunsaturated fatty acids (PUFA) supplementation on plasma and aortic nitric oxide (NO) levels in isoproterenol (Iso) affected spontaneously hypertensive (SHR) and Wistar rats. Untreated control rats were compared with Iso injected (118 mg/kg, s.c.) rats, and Iso injected plus supplemented with melatonin (10 mg/kg, p.o.) or PUFA (1.68 g/kg, p.o.) for two months. Plasma and aortic basal, L-NAME inhibited, adrenaline and acetylcholine stimulated NO were determined using Griess method. Plasma NO levels were lower in SHR versus Wistar rats. Iso decreased NO in Wistar while not in SHR. PUFA but not melatonin intake of Iso treated SHR increased plasma NO along with a decrease in systolic blood pressure. Basal aortic NO level was higher in SHR than Wistar rats and not altered by Iso. Intake of melatonin increased but PUFA decreased basal NO levels in Wistar+Iso and did not affect in SHR+Iso rats. Acetylcholine and adrenaline induced aortic NO release was significantly increased in Wistar+Iso but not SHR+Iso group. Melatonin intake increased Ach induced aortic NO in Wistar+Iso and SHR+Iso groups, whereas there was no effect of PUFA intake. Findings suggest that PUFA modulates plasma and melatonin aortic NO levels of isoproterenol affected rats in a strain-dependent manner.

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Regulation of Hypothalamic-Pituitary-Adrenal Axis by Circulating Free Fatty Acids in Male Wistar Rats: Role of Individual Free Fatty Acids

Endocrinology ◽

10.1210/en.2013-1700 ◽

2014 ◽

Vol 155 (3) ◽

pp. 923-931 ◽

Cited By ~ 18

Author(s):

Young Taek Oh ◽

Jinyub Kim ◽

Insug Kang ◽

Jang H. Youn

Keyword(s):

Fatty Acids ◽

Free Fatty Acids ◽

Wistar Rats ◽

Hypothalamic Pituitary Adrenal Axis ◽

Hypothalamic Pituitary Adrenal ◽

Adrenal Axis ◽

Male Wistar Rats ◽

Pituitary Adrenal Axis

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A Mixture of Algae and Extra Virgin Olive Oils Attenuates the Cardiometabolic Alterations Associated with Aging in Male Wistar Rats

Antioxidants ◽

10.3390/antiox9060483 ◽

2020 ◽

Vol 9 (6) ◽

pp. 483 ◽

Cited By ~ 2

Author(s):

Daniel González-Hedström ◽

Sara Amor ◽

María de la Fuente-Fernández ◽

Antonio Tejera-Muñoz ◽

Teresa Priego ◽

...

Keyword(s):

Fatty Acids ◽

Insulin Sensitivity ◽

Vascular Function ◽

Wistar Rats ◽

Unsaturated Fatty Acids ◽

Saturated Fatty Acids ◽

Virgin Olive Oil ◽

Mrna Levels ◽

Serum Concentrations ◽

Male Wistar Rats

Aging is one of the major risk factors for suffering cardiovascular and metabolic diseases. Due to the increase in life expectancy, there is a strong interest in the search for anti-aging strategies to treat and prevent these aging-induced disorders. Both omega 3 polyunsaturated fatty acids (ω-3 PUFA) and extra virgin olive oil (EVOO) exert numerous metabolic and cardiovascular benefits in the elderly. In addition, EVOO constitutes an interesting ingredient to stabilize ω-3 PUFA and decrease their oxidation process due to its high content in antioxidant compounds. ω-3 PUFA are commonly obtained from fish. However, more ecological and sustainable sources, such as algae oil (AO) can also be used. In this study, we aimed to study the possible beneficial effect of an oil mixture composed by EVOO (75%) and AO (25%) rich in ω-3 PUFA (35% docosahexaenoic acid (DHA) and 20% eicosapentaenoic acid (EPA)) on the cardiometabolic alterations associated with aging. For this purpose; young (three months old) and old (24 months old) male Wistar rats were treated with vehicle or with the oil mixture (2.5 mL/kg) for 21 days. Treatment with the oil mixture prevented the aging-induced increase in the serum levels of saturated fatty acids (SFA) and the aging-induced decrease in the serum concentrations of mono-unsaturated fatty acids (MUFA). Old treated rats showed increased serum concentrations of EPA and DHA and decreased HOMA-IR index and circulating levels of total cholesterol, insulin and IL-6. Treatment with the oil mixture increased the mRNA levels of antioxidant and insulin sensitivity-related enzymes, as well as reduced the gene expression of pro-inflammatory markers in the liver and in cardiac and aortic tissues. In addition, the treatment also prevented the aging-induced endothelial dysfunction and vascular insulin resistance through activation of the PI3K/Akt pathway. Moreover, aortic rings from old rats treated with the oil mixture showed a decreased response to the vasoconstrictor AngII. In conclusion, treatment with a mixture of EVOO and AO improves the lipid profile, insulin sensitivity and vascular function in aged rats and decreases aging-induced inflammation and oxidative stress in the liver, and in the cardiovascular system. Thus, it could be an interesting strategy to deal with cardiometabolic alterations associated with aging.

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Maternal caloric restriction spares fetal brain polyunsaturated fatty acids in Wistar rats

Brain and Development ◽

10.1016/j.braindev.2008.12.001 ◽

2010 ◽

Vol 32 (2) ◽

pp. 123-129 ◽

Cited By ~ 6

Author(s):

Sonali Agale ◽

Asmita Kulkarni ◽

Prabhakar Ranjekar ◽

Sadhana Joshi

Keyword(s):

Fatty Acids ◽

Polyunsaturated Fatty Acids ◽

Caloric Restriction ◽

Wistar Rats ◽

Fetal Brain

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Prophylactic and therapeutic effects of Mytilus edulis fatty acids on adjuvant-induced arthritis in male Wistar rats

Prostaglandins Leukotrienes and Essential Fatty Acids ◽

10.1016/j.plefa.2009.12.003 ◽

2010 ◽

Vol 82 (2-3) ◽

pp. 97-103 ◽

Cited By ~ 8

Author(s):

Sarah McPhee ◽

Lynn D. Hodges ◽

Paul F.A. Wright ◽

Paul M. Wynne ◽

Nicolette Kalafatis ◽

...

Keyword(s):

Fatty Acids ◽

Mytilus Edulis ◽

Wistar Rats ◽

Therapeutic Effects ◽

Adjuvant Induced Arthritis ◽

Male Wistar Rats

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Hepatoprotective effect of red ginger rhizome extract in deep frying oil-fed male wistar rats

Zinc supplementation improves heme biosynthesis in rats exposed to lead ◽

10.18051/univmed.2017.v36.228-235 ◽

2017 ◽

Vol 36 (3) ◽

pp. 228 ◽

Cited By ~ 1

Author(s):

Sunarti Sunarti ◽

Edy Fachrial ◽

Urip Harahap ◽

Delyuzar Delyuzar ◽

Tri Widyawati ◽

...

Keyword(s):

Fatty Acids ◽

Wistar Rats ◽

Fatty Degeneration ◽

Frying Oil ◽

Deep Frying ◽

Treatment Groups ◽

Whitney Test ◽

Ginger Rhizome ◽

Male Wistar Rats ◽

Red Ginger

BACKGROUND Non-alcoholic steatohepatitis (NASH) is a chronic liver disease associated with increased free fatty acids, trans fatty acids and free radical compounds due to the consumption of deep frying oil (DFO). Ginger has hypolipidemic and antioxidant properties, and acts as an insulin sensitizer. The present study was conducted to evaluate the hepatoprotective effect of an ethanol extract of Zingiber officinale Roxb var rubrum (EEZO) in DFO-fed male Wistar rats. METHODS A study of experimental design was conducted involving 24 non-alcoholic steatohepatitis (NASH) male Wistar rats that were randomly divided into 4 groups. Group K1 (control) was given DFO-3 (3 times heated DFO) at a dose of 10 ìl/g BW/day. The treatment groups (P1, P2, P3) were given DFO-3 and red ginger rhizome extract at doses of 100, 200, and 400 mg/kg BW orally for 30 days. The histopathology examination assessed fatty degeneration, and degree of fibrosis. The data were analyzed with the Kruskal-Wallis and Mann-whitney test. RESULTS The Kruskal-Wallis test showed that fatty degeneration (steatosis, lobular inflammation, hepatocyte ballooning and NASH) were significantly different among the four groups (p<0.05). Mann-Whitney test proved that fatty degeneration was not significantly different between control and treatment groups (p>0.05). CONCLUSION Deep frying oil increased fatty degeneration and NASH, and none of red ginger rhizome extract doses was able to inhibit fatty degeneration significantly. For the future research it is intended to prolong the induction time and the administration of red ginger rhizome extract.

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Dietary supplementation with monounsaturated and long-chain polyunsaturated fatty acids influences the liver structural recovery and hepatocyte binuclearity in female Wistar rats in experimental cirrhosis induced by thioacetamide

Experimental and Toxicologic Pathology ◽

10.1016/j.etp.2005.02.001 ◽

2005 ◽

Vol 57 (1) ◽

pp. 65-75 ◽

Cited By ~ 5

Author(s):

I. Fernández ◽

L. Fontana ◽

A. Gil ◽

A. Ríos ◽

M.I. Torres

Keyword(s):

Fatty Acids ◽

Polyunsaturated Fatty Acids ◽

Wistar Rats ◽

Dietary Supplementation ◽

Experimental Cirrhosis ◽

Structural Recovery ◽

Female Wistar Rats ◽

Long Chain

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Marine n-3 fatty acids promote size reduction of visceral adipose depots, without altering body weight and composition, in male Wistar rats fed a high-fat diet

British Journal Of Nutrition ◽

10.1017/s0007114509353210 ◽

2009 ◽

Vol 102 (7) ◽

pp. 995-1006 ◽

Cited By ~ 40

Author(s):

Merethe H. Rokling-Andersen ◽

Arild C. Rustan ◽

Andreas J. Wensaas ◽

Olav Kaalhus ◽

Hege Wergedahl ◽

...

Keyword(s):

Fatty Acids ◽

Mrna Expression ◽

High Fat Diet ◽

Wistar Rats ◽

Plasma Lipid ◽

Feed Consumption ◽

High Fat ◽

Male Wistar Rats ◽

Similar Body ◽

Visceral Adipose

We evaluated the effects of partly substituting lard with marine n-3 fatty acids (FA) on body composition and weight, adipose tissue distribution and gene expression in five adipose depots of male Wistar rats fed a high-fat diet. Rats were fed diets including lard (19·5 % lard) or n-3 FA (9·1 % lard and 10·4 % Triomar™) for 7 weeks. Feed consumption and weight gain were similar, whereas plasma lipid concentrations were lower in the n-3 FA group. Magnetic resonance imaging revealed smaller visceral (mesenteric, perirenal and epididymal) adipose depots in the n-3 FA-fed animals (35, 44 and 32 % reductions, respectively). n-3 FA feeding increased mRNA expression of cytokines as well as chemokines in several adipose depots. Expression of Adipoq and Pparg was enhanced in the mesenteric adipose depots of the n-3 FA-fed rats, and fasting plasma insulin levels were lowered. Expression of the lipogenic enzymes Acaca and Fasn was increased in the visceral adipose depots, whereas Dgat1 was reduced in the perirenal and epididymal depots. Cpt2 mRNA expression was almost doubled in the mesenteric depot and liver. Carcass analyses showed similar body fat (%) in the two feeding groups, indicating that n-3 FA feeding led to redistribution of fat away from the visceral compartment.

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