IN VITRO SCREENING OF LANNEA COROMANDELICA FOR ANTIFILARIAL ACTIVITY ON CATTLE FILARIAL PARASITE SETARIA CERVI

INDIAN DRUGS ◽  
2014 ◽  
Vol 51 (08) ◽  
pp. 28-34
Author(s):  
T. Kumar ◽  
◽  
A Sahu ◽  
V Jain
Keyword(s):  
Ethyl Acetate ◽  
Aqueous Extract ◽  
Ethyl Acetate Extract ◽  
Muscle Preparation ◽  
Setaria Cervi ◽  
Lannea Coromandelica ◽  
Nerve Muscle ◽  
Antifilarial Activity ◽  
Ethyl Acetate Extracts

The current study was aimed to investigate the antifilarial activity of aqueous and ethyl acetate extracts of the leaves of Lannea coromandelica. Both extracts were screened for their total flavonoid content chromatographically. The total amount of flavonoid present in ethyl acetate is much greater than in aqueous extract. Aqueous extract produced initial stimulation effect in whole worm followed by reversible paralysis, whereas on the nerve muscle preparation, the effect was depressant. In the same manner ethyl acetate extract produces irreversible paralysis. The concentrations required to inhibit the movement of the whole worm and nerve muscle preparation for aqueous extract of leaves of L. coromandelica were 300 and 60 µg/mL, respectively, whereas ethyl acetate extract caused inhibition of the whole worm and nerve muscle preparation at 160 and 15 µg/mL, respectively. LC50 and LC90 were 21 and 30 ng/mL, respectively for aqueous, which were 16 and 24 ng/mL for ethyl acetate extracts.

scholarly journals Effects of different extracts of Cremastra appendiculata (D. Don) Makino Cremastra appendiculata (D. Don) Makino on apoptosis of A549 cells

2020 ◽  
Vol 19 (8) ◽  
pp. 1653-1659
Author(s):  
Haifeng Zhang ◽  
Ziying Zhang ◽  
Hua Du ◽  
Xiaoli Su ◽  
Xiaorong Li ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Ethyl Acetate ◽  
Mtt Assay ◽  
High Performance ◽  
Ethyl Acetate Extract ◽  
A549 Cells ◽  
Butanol Extract ◽  
Cremastra Appendiculata ◽  
Ethyl Acetate Extracts

Purpose: To investigate the effect of different extracts of Cremastra appendiculata (D. Don) Makino onapoptosis of A549 cells, and the underlying mechanism.Methods: The contents of colchicine in ethyl acetate and n-butanol extracts of Cremastra appendiculata(D. Don) Makino were determined using high performance liquid chromatography (HPLC). Lung cancerA549 cells cultured in vitro were divided into blank control, standard colchicine and Cremastra appendiculata (D. Don) Makino extract groups. The effect of different extract concentrations on proliferation of the cells was determined using methyl thiazolyl diphenyl-tetrazolium (MTT) assay, while apoptosis of A549 cells induced by the extracts was evaluated by flow cytometry (FC).Results: Compared with the standard colchicine group, there was no colchicine in the n-butanol and ethyl acetate extracts of Cremastra appendiculata. Results from MTT assay showed that the extract inhibited the proliferation of A549 cells (p < 0.05). Flow cytometry results showed that ethyl acetate extract significantly enhanced apoptosis in A549 cells (p < 0.05). However, n-butanol extract had no significant effect on the apoptosis of A549 cells (p < 0.05).Conclusion: The ethyl acetate extract of Cremastra appendiculata (D. Don) Makino induces apoptosis in lung cancer A549 cells. Therefore, there is a need for further research and development of antitumor drugs from the extract of Cremastra appendiculata (D. Don) Makino. Keywords: Cremastra appendiculata (D. Don) Makino, Colchicine, A549 cells, Apoptosis

scholarly journals <i>In-vitro</i> antibacterial sensitivity of <i>Usnea barbata</i> lichen extracted with methanol and ethyl-acetate against selected <i>Staphylococcus</i> species from milk of cows with mastitis

2014 ◽  
Vol 57 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Emrobowansan Monday Idamokoro ◽  
Patrick Julius Masika ◽  
Voster Muchenje ◽  
Daniel Falta ◽  
Ezekiel Green
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Bovine Milk ◽  
Raw Milk ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Ethyl Acetate Extracts

Abstract. This study aimed at evaluating the antimicrobial potential of Usnea barbata lichen as a medicinal plant against selected Staphylococcus species isolated from raw milk of cows. In-vitro screening of methanol and ethyl-acetate extracts from Usnea barbata lichen were evaluated to determine their antimicrobial activity against thirteen different Staphylococcus species. The selected organisms were isolated from raw bovine milk and identified using several biochemical tests and confirmed with API staph kit. The antimicrobial activity of the extracts were evaluated using both the agar well diffusion method (at 5 mg/ml, 10 mg/ml and 20 mg/ml) and the broth micro-dilution technique to determine the mean zone of inhibition and the minimum inhibitory concentration (MIC), respectively. Both the methanol and ethyl-acetate extracts showed variable antimicrobial activity against the Staphylococcus species with mean zones of inhibition ranging from 0-34 mm in diameter at 5 mg/ml, 10 mg/ml and 20 mg/ml, respectively. Susceptibility by the Staphylococcus species tested in the methanol and the ethyl-acetate extract was 92.31 % and 53.85 %, respectively. The MIC result for the methanol extract ranged from 0.04 to 10 mg/ml, while that of the ethyl-acetate extract ranged from 0.16 to 5 mg/ml. Results from this study revealed the in vitro microbial activity of Usnea barbata extracts which indicate its potential as a medicinal plant.

scholarly journals ISOLASI DAN IDENTIFIKASI SENYAWA ANTIOKSIDAN GOLONGAN FLAVONOID DARI EKSTRAK ETIL ASETAT DAUN PRANAJIWA (Euchresta horsfieldii Lesch Benn.)

2017 ◽  
Vol 5 (1) ◽  
pp. 26
Author(s):  
Ni Wayan Rika Kumara Dewi ◽  
I Wayan Gunawan ◽  
Ni Made Puspawati
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Infrared Spectra ◽  
Ethyl Acetate Extract ◽  
Total Flavonoid ◽  
Separation And Purification ◽  
Ic50 Value ◽  
Ethyl Acetate Extracts ◽  
In Vitro Antioxidant Activity

ABSTRAK: Penelitian ini bertujuan untuk menentukan aktivitas antioksidan dan mengidentifikasi senyawa golongan flavonoid yang terkandung dalam ekstrak etil asetat daun pranajiwa (Euchresta horsfieldii Lesch Benn.). Uji aktivitas antioksidan dilakukan secara invitro dengan metode DPPH (1,1-diphenyl-2-pycrylhidrazyl). Teknik pemisahan dilakukan dengan kromatografi kolom dan diidentifikasi dengan spektrofotometer FTIR dan UV-Vis. Hasil penelitian menunjukkan bahwa ekstrak etil asetat memiliki aktivitas antioksidan dengan nilai IC50 sebesar 393,95 µg/mL dan kadar total flavonoid sebesar 6619,72 mg QE/100g atau 6,62 % QE. Pemisahan dan pemurnian ekstrak etil asetat menggunakan fase diam silika gel 60 dan fase gerak n-heksana:kloroform: etanol (20:1:1) yang menghasilkan isolat aktif yang positif flavonoid (isolat FE) yang diduga merupakan senyawa flavonoid golongan flavonol yaitu 3,5,7,3’,4’-pentahidroksi flavonol.   ABSTRACT: The present study was conducted to determine antioxidant activity and to identify the flavonoid active compounds from ethyl acetate extracts of Pranajiwa leaves. In vitro antioxidant activity was carried out using DPPH (1,1-diphenyl-2-pycrylhidrazyl) method. The separation of the ethyl acetate extracts was conducted by column chromatography and the identification by using FTIR and UV-Vis spectrophotometer. The results showed that ethyl acetate extract has antioxidant activity with IC50 value of 393,95 µg/mL and the total flavonoid of 6619,72 mg QE /100g or 6,62% QE. The separation and purification of  ethyl acetate extract was chromatography column on silica gel 60 and the solvent n-hexane:chloroform:ethanol (20:1:1) based on UV-Vis and Infrared spectra positive flavonoid isolate (isolates FE) was tentaviely identified as 3,5,7,3',4'-pentadihydroxy flavonols.    

scholarly journals Phenolic Compounds and Bioactivity of Cytisus villosus Pourr.

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1994 ◽  
Author(s):  
Amel Bouziane ◽  
Boulanouar Bakchiche ◽  
Maria Dias ◽  
Lillian Barros ◽  
Isabel Ferreira ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Ethyl Acetate Extract ◽  
Pathogenic Fungus ◽  
Antimicrobial Activities ◽  
Gram Positive ◽  
Aerial Parts ◽  
Ethyl Acetate Extracts

The present study focuses on the chemical composition, antioxidant, antimicrobial, and antiproliferative activities of the ethyl acetate and aqueous extracts obtained from the aerial parts of Cytisus villosus Pourr. HPLC-DAD-ESI/MSn was used to identify the phenolic compounds, being (epi)gallocatechin dimer the major compound (111 ± 5 µg/g·dw) in the aqueous extract, while myricetin-O-rhamnoside (226 ± 9 µg/g·dw) was the main molecule in the ethyl acetate extract. Both extracts exhibited good scavenging activities against DPPH radical (EC50 µg/mL of 59 ± 2 and 31 ± 2 for aqueous and ethyl acetate extracts, respectively). However, the ethyl acetate extract demonstrated more potent quenching activities than the aqueous extract. The antimicrobial activities were assessed on selected Gram-positive (Staphylococcus epidermidis) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria, as well as on pathogenic fungus Candida glabrata. The extracts possessed selective and potent antimicrobial activities against the Gram-positive bacterium (IC50 of 186 ± 9 μg/mL and 92 ± 3 μg/mL for aqueous and ethyl acetate extracts, respectively). Finally, C. villosus extracts were evaluated for their antiproliferative potential on three human cancer cell lines representing breast and colon cancers. Although both extracts demonstrated sufficient growth inhibition of the three different cell lines, the ethyl acetate extract exhibited higher activity (LD50 values of 1.57 ± 0.06 mg/mL, 2.2 ± 0.1 mg/mL, and 3.2 ± 0.2 mg/mL for T47D, MCF-7, and HCT-116 cell lines). Both the extracts obtained from the aerial parts of C. villosus revealed very promising results and could be applied as functional agents in the food, pharmaceutical, and cosmeceutical industries.

scholarly journals Antileishmanial and cytotoxic activities of four Andean plant extracts from Colombia

2020 ◽  
Vol 13 (10) ◽  
pp. 2178-2182
Author(s):  
Wilson Cardona-G ◽  
Sara Robledo ◽  
Fernando Alzate ◽  
Andrés F. Yepes ◽  
Cristian Hernandez ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Plant Extracts ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Cytotoxic Activities ◽  
Moderate Activity ◽  
Leishmania Panamensis ◽  
Ethanol Extracts ◽  
Ethyl Acetate Extracts

Background and Aim: Licania salicifolia (L.S) Cuatrec., Persea ferruginea (P.F) Kunth, Oreopanax floribundus (O.F), and Psychotria buchtienii (P.B) belong to the families Chrysobalanaceae, Lauraceae, Araliaceae, and Rubiaceae, respectively, which have been used as medicines by communities in the Andes. This study evaluated the leishmanicidal and cytotoxic activities of alcohol and non-alcohol extracts from four Andean plant extracts (L.S, O.F, P.F, and P.B). Materials and Methods: Extracts were obtained by percolation with solvents of different polarities – hexane, dichloromethane, ethyl acetate, and ethanol. Phytochemical screening was conducted based on reported methods. All products were evaluated in vitro to determine the leishmanicidal activity against amastigotes of Leishmania panamensis and cytotoxicity against U937 cells. Results: Flavonoids, triterpenes, and tannins were the main secondary metabolites found. From the results, dichloromethane extracts from O.F and P.B, ethanol extract from P.B, and ethyl acetate extracts of all plants were active, with EC50 <30 μg/mL. Ethyl acetate was the most active extract, which showed EC50 values of 9.8, 14.1, 23.7, and 25.5 μg/mL, for L.S, P.B, O.F, and P.F, respectively. Hexane extracts from P.B and O.F exhibited moderate activity with EC50 values of 84.8 and 87.4 μg/mL, respectively. Hexane and ethanol extracts from O.F, ethyl acetate, and ethanol extracts from L.S, and all extracts from P.F were not toxic. Alternatively, hexane and dichloromethane extracts from L.S and P.B as well as dichloromethane and ethyl acetate extracts from O.F displayed high toxicity. Conclusion: Based on the activity we observed, ethyl acetate extract can continue in its usage in the search for new antileishmanial drugs, mainly ethyl acetate extract from L.S showed activity comparable to meglumine antimoniate and was not cytotoxic.

Effect of Chroogomphis Rutillus Extracts on Activity of Nitrite Scavenging and Nitrosamine Formation Inhibition

2011 ◽  
Vol 282-283 ◽  
pp. 545-548
Author(s):  
Hong Fang Ji ◽  
Ling Wen Zhang ◽  
Hui Hui Jin ◽  
Ling Yu Wang ◽  
Fang Yuan Cheng ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethyl Acetate Extract ◽  
Water Extract ◽  
Dependent Manner ◽  
In Vitro Systems ◽  
Dose Dependent ◽  
Ethyl Acetate Extracts ◽  
Scavenging Rates

Chroogomphis rutillusextracts were prepared with solvent of petroleum ether, acetic ester, methanol and water. The effects of four different extract on nitrite scavenging (NS) and nitrosamine formation inhibiting (NFI) were evaluated in vitro systems. Results showed that all the extract showed NS and NFI potentials with a dose-dependent manner. With regard to NS activity, at 50μg/mL, the scavenging rates of ethyl acetate and methanol extracts were 96.65% and 93.48%, respectively. While, those of petroleum ether and water extract were only 86.96% and 26.09%, respectively. With regard to NFI activity,at 200μg/mL, both petroleum ether and ethyl acetate extracts showed excellent inhibition abilities of 94.46% and 91.33%, respectively, much higher than those of methanol and water extract (78.57% and 4.06%, respectively) (P<0.05).Chroogomphis rutillusespecially ethyl acetate extract may have a preventive effect against carcinogenesis induced by nitrosamines.

scholarly journals A Comparative Study of in vivo Plant and in vitro Callus Extracts of Centratherum punctatum Cass

2020 ◽  
pp. 1-13
Author(s):  
K. Mercy Madhumitha ◽  
J. Anbumalarmathi ◽  
S. Aruna Sharmili ◽  
G. Nandhini ◽  
G. Shanmuga Priya
Keyword(s):  
Comparative Study ◽  
Ethyl Acetate ◽  
Aqueous Extract ◽  
Plant Extract ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition

The present study aims at comparative study between plant and callus extract, with respect to analysis of phytochemical constituents, antioxidant, antibacterial activity and cytotoxicity properties of Centratherum punctatum using aqueous medium and different solvents such as, methanol and ethyl acetate. In vitro studies in MS media supplemented with BAP 4.5 mg/L + Kn 4.0 mg/L has shown the high callus induction percentage of 92.33% with a maximum callus weight of 1.08 g. The phytochemical analysis of aqueous, methanol and ethyl acetate extract of C. punctatum in vivo plant and in vitro callus showed the presence of alkaloids, flavonoids, phenols and carbohydrates. The aqueous extract of both plant and callus showed the presence of tannins, proteins and steroids whereas the methanol extract showed the presence of tannins, amino acids and terpenoids. The ethyl acetate extract showed terpenoids and protein. FTIR analysis of plant and callus aqueous extract had a maximum characteristic band at 3399.87 cm-1 and 3412.73 cm-1 respectively indicating the presence of N-H stretching. GC-MS analysis revealed the presence of 11 different compounds in ethyl acetate extracts of plant and the callus extract revealed the presence of 15 different compounds which was absent in the plant extract. Plant extract exhibited maximum total phenol content than callus extract. The in vitro callus extract showed higher DPPH radical scavenging activity with lower inhibition percentage than in vivo plant extract. A maximum zone of inhibition was observed in methanol extract of in vivo plant and in vitro callus (15 mm and 14 mm respectively) against Bacillus subtilis. The ethyl acetate extract of in vivo plant and in vitro callus had a zone of 14 mm and 12 mm against E. coli. A maximum zone of inhibition (12 mm and 11 mm respectively) was observed in both methanol and ethyl acetate of in vivo plant and in vitro callus against Staphylococcus aureus. Antiproliferative analysis revealed that in vivo plant has inhibitory percentage of 23.6 whereas callus exhibited 28.5% against HeLa cells.

scholarly journals Antimicrobial and in vitro cytotoxic studies of Acampe praemorsa and Aeridis odarata of Orchidaceae

2018 ◽  
Vol 7 (2) ◽  
pp. 2088
Author(s):  
Jhansi Katta ◽  
Khasim S.M.
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Eastern Ghats ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Mcf 7 ◽  
Ethyl Acetate Extracts

This paper aims at studying antimicrobial efficacy and in vitro cytoxic activity of epiphytic orchids namely Acampe praemorsa, Aeridis odorata distributed in Eastern Ghats of Visakhapatnam district. Plants were collected, identified following literature, shade dried and Methanol, ethyl acetate extracts were prepared for the systematic investigation of antimicrobial activity of plant extracts. Antibacterial activity against three gram positive bacteria Bacillus megaterium, Lactobacillus acidophillus and Enterococcus faecalis, three gram negative bacteria Proteus vulgaris, Klebsiella pneumoniae and Escherichia coli was done using Agar well diffusion method, antifungal activity was carried against Candida albicans, Aspergillus flavus by Cpzapak dox agar media, MIC by broth dilution method, zones of inhibition were recorded. Ethyl acetate extracts showed maximum antimicrobial activity against all bacteria and fungi, Aeridis odarata ethyl acetate extract showed highest zone of inhibition 17mm against Lactobacillus acidophilus in bacteria, Ethyl acetate extract of Acampe praemorsa showed highest zone of inhibition 17mm against Candida albicans in fungi, the leaf extracts were tested for its inhibitory effect on HeLa and MCF-7 cell lines were evaluated by the MTT assay and methanolic extract of Aeridis odarata has significant cytotoxicity effect on MCF-7 cell line in concentration range between 5 to 100µg/ml, with IC 50 (µg/ml) value is 26.2. These plants have good antimicrobial activity, further investigation on the phytochemistry of bioactive compounds of these plants would result in discovery of new drugs and further pharmacological investigation of anti-cancer activity of Aeridis odarata should be done.

In-vitro bactericidal activity of a novel plant source Plumeria pudica against some human and fish pathogenic bacteria

2020 ◽  
Vol 17 ◽  
Author(s):  
Shubhaisi Das ◽  
Sunanda Burman ◽  
Goutam Chandra
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Positive Control ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Ft Ir ◽  
Aldehyde Groups

Background: The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin. Objective: The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica. Methods: Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically. Results: Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract five different bioactive compounds e.g., 2,4-ditert –butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified. Conclusion: Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.

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