Effects of low ambient temperatures and dietary vitamin C supplementation on pulmonary vascular remodeling and hypoxic gene expression of 21-d-old broilers

Objective: Since sperm abnormalities are known to be a major reason for recurrent pregnancy loss (RPL), any defects in DNA structure and chromatin condensation can place embryos at risk in the early stage of development and implantation. As antioxidants such as vitamin C may play a protective role against the destruction of protamine genes in sperm chromatin, this study was conducted to evaluate the effects of vitamin C on chromatin and the expression of protamine genes in the male partners of couples with RPL.Methods: Twenty male partners of couples with RPL were selected as the intervention group and received vitamin C supplementation (250 mg daily for 3 months). Healthy fertile men (n=20) were included as controls. Sperm chromatin, DNA integrity, and the expression levels of protamine genes were evaluated before and after treatment.Results: Significant differences were found in sperm morphology, protamine deficiency, and apoptosis between the two groups and before and after vitamin C administration. A significant change was found in mRNA levels of <i>PRM1, PRM2</i>, and the <i>PRM1/PRM2</i> ratio after treatment.Conclusion: Daily oral administration of vitamin C may improve human sperm parameters and DNA integrity by increasing protamine gene expression levels in the male partners of couples with RPL. The beneficial effects of vitamin C supplementation as an antioxidant for the male partners of couples with RPL could lead to improved pregnancy outcomes in these cases.

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Effects of low ambient temperatures and dietary vitamin C supplement on growth performance, blood parameters, and antioxidant capacity of 21-day-old broilers

Poultry Science ◽

10.3382/ps.2013-03438 ◽

2014 ◽

Vol 93 (4) ◽

pp. 898-905 ◽

Cited By ~ 15

Author(s):

X. Yang ◽

Y.H. Luo ◽

Q.F. Zeng ◽

K.Y. Zhang ◽

X.M. Ding ◽

...

Keyword(s):

Vitamin C ◽

Antioxidant Capacity ◽

Growth Performance ◽

Blood Parameters ◽

Dietary Vitamin ◽

Ambient Temperatures

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Longitudinal changes in serum paraoxonase-1 activity throughout normal pregnancy

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm.2006.156 ◽

2006 ◽

Vol 44 (7) ◽

Cited By ~ 10

Author(s):

Natàlia Ferré ◽

Jordi Camps ◽

Joan Fernández-Ballart ◽

Victoria Arija ◽

Michelle M. Murphy ◽

...

Keyword(s):

Vitamin C ◽

Normal Pregnancy ◽

Paraoxonase 1 ◽

Dietary Vitamin ◽

Pon1 Activity ◽

Longitudinal Changes ◽

Increased Risk ◽

Direct Association ◽

Vitamin C Supplementation ◽

Serum Paraoxonase

AbstractThe aim of this study was to investigate the longitudinal changes in serum paraoxonase-1 (PON1) activity from preconception throughout normal pregnancy and their relationships with maternal dietary vitamin C and E intake. The study was performed in 35 women (studied at preconception, at 8, 20 and 32weeks of pregnancy, and at labour). PON1 activity decreased significantly from 145.8 (109.8–198.8) U/L at preconception to 111.1 (85.3–179.9) U/L (p<0.01) at 32weeks and 100.4 (54.7–171.4) U/L (p<0.001) at labour. There was a direct association between vitamin C intake and PON1 at week 32 (p=0.018). We conclude that adequate vitamin C intake in pregnant women may merit consideration, since vitamin C supplementation has proved beneficial in the prevention of preeclampsia in women at increased risk of this condition.Clin Chem Lab Med 2006;44:880–2.

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Dietary vitamin C supplementation reduces noise-induced hearing loss in guinea pigs

Hearing Research ◽

10.1016/j.heares.2004.10.011 ◽

2005 ◽

Vol 202 (1-2) ◽

pp. 200-208 ◽

Cited By ~ 54

Author(s):

Sandra L. McFadden ◽

Jenifer M. Woo ◽

Nathan Michalak ◽

Dalian Ding

Keyword(s):

Hearing Loss ◽

Vitamin C ◽

Guinea Pigs ◽

Dietary Vitamin ◽

Noise Induced Hearing Loss ◽

Vitamin C Supplementation

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Vitamin C intake and susceptibility to the common cold

British Journal Of Nutrition ◽

10.1017/s0007114500002889 ◽

1997 ◽

Vol 77 (1) ◽

pp. 59-72 ◽

Cited By ~ 60

Author(s):

Harri Hemilä

Keyword(s):

Vitamin C ◽

Common Cold ◽

Potential Effect ◽

Dietary Vitamin ◽

High Dose ◽

Plasma Vitamin ◽

Vitamin C Supplementation ◽

The Common ◽

The Uk

Although the role of vitamin C in common cold incidence had been studied extensively, the level of vitamin C intake has not been unequivocally shown to affect the incidence of colds. In the present study the six largest vitamin C supplementation (≥ 1 g/d) studies, including over 5000 episodes in all, have been analysed, and it is shown that common cold incidence is not reduced in the vitamin C-supplemented groups compared with the placebo groups (pooled rate ratio (RR) 0·99; 95% CI 0·93, 1·04). Consequently these six major studies give no evidence that high-dose vitamin C supplementation decreases common cold incidence in ordinary people. Nevertheless, the analysis was continued with the hypothesis that vitamin C intake may affect common cold susceptibility in specific groups of people. It was assumed that the potential effect of supplementation might be most conspicuous in subjects with low dietary vitamin C intake. The average vitamin C intake has been rather low in the UK and plasma vitamin C concentrations are in general lower in males than in females. In four studies with British females vitamin C supplementation had no marked effect on common cold incidence (pooled RR 0·95; 95% CI 0·86, 1·04). However, in four studies with British male schoolchildren and students a statistically highly significant reduction in common cold incidence was found in groups supplemented with vitamin C (pooled RR 0·70; 95% CI 0·60, 0·81). Thus, these studies with British males indicate that vitamin C intake has physiological effects on susceptibility to common cold infections, although the effect seems quantitatively meaningful only in limited groups of people and is not very large.

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Delineating the angiogenic gene expression profile before pulmonary vascular remodeling in a lamb model of congenital heart disease

Physiological Genomics ◽

10.1152/physiolgenomics.00135.2010 ◽

2011 ◽

Vol 43 (2) ◽

pp. 87-98 ◽

Cited By ~ 9

Author(s):

Jing Tian ◽

Sohrab Fratz ◽

Yali Hou ◽

Qing Lu ◽

Agnes Görlach ◽

...

Keyword(s):

Gene Expression ◽

Shear Stress ◽

Gene Expression Profile ◽

Expression Profile ◽

Vascular Remodeling ◽

Neutralizing Antibodies ◽

Congenital Heart ◽

Heart Diseases ◽

Pulmonary Vascular Remodeling ◽

Angiogenic Response

Disordered angiogenesis is implicated in pulmonary vascular remodeling secondary to congenital heart diseases (CHD). However, the underlying genes are not well delineated. We showed previously that an ovine model of CHD with increased pulmonary blood flow (PBF, Shunt) has an “angiogenesis burst” between 1 and 4 wk of age. Thus we hypothesized that the increased PBF elicited a proangiogenic gene expression profile before onset of vessel growth. To test this we utilized microarray analysis to identify genes that could be responsible for the angiogenic response. Total RNA was isolated from lungs of Shunt and control lambs at 3 days of age and hybridized to Affymetrix gene chips for microarray analyses ( n = 8/group). Eighty-nine angiogenesis-related genes were found to be upregulated and 26 angiogenesis-related genes downregulated in Shunt compared with control lungs (cutting at 1.2-fold difference, P < 0.05). We then confirmed upregulation of proangiogenic genes FGF2, Angiopoietin2 (Angpt2), and Birc5 at mRNA and protein levels and upregulation of ccl2 at mRNA level in 3-day Shunt lungs. Furthermore, we found that pulmonary arterial endothelial cells (PAEC) isolated from fetal lambs exhibited increased expression of FGF2, Angpt2, Birc5, and ccl2 and enhanced angiogenesis when exposed to elevated shear stress (35 dyn/cm2) compared with cells exposed to more physiological shear stress (20 dyn/cm2). Finally, we demonstrated that blocking FGF2, Angpt2, Birc5, or ccl2 signaling with neutralizing antibodies or small interfering RNA (siRNA) significantly decreased the angiogenic response induced by shear stress. In conclusion, we have identified a “proangiogenic” gene expression profile in a lamb model of CHD with increased PBF that precedes onset of pulmonary vascular remodeling. Our data indicate that FGF2, Angpt2, Birc5, and ccl2 may play important roles in the angiogenic response.

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Effects of dietary vitamin C supplementation on fatty acid profile in breast meat of broiler chickens

Meso ◽

10.31727/m.22.4.4 ◽

2020 ◽

Vol 22 (4) ◽

pp. 268-273

Author(s):

Masoomeh Tavakoli ◽

Mehrdad Bouyeh ◽

Alireza Seidavi

Keyword(s):

Fatty Acid ◽

Vitamin C ◽

Fatty Acid Profile ◽

Broiler Chickens ◽

Dietary Vitamin ◽

Breast Meat ◽

Vitamin C Supplementation

Ziel der Studie war es, die Auswirkungen der Aufnahme von drei verschiedenen Vitamin C-Mengen (0, 200 und 400 mg / kg) in die Ernährung auf das Fettsäureprofil im Broilerbrustfleisch zu bewerten. Die Studie wurde in 3 Zyklen und 4 Wiederholungen in Dauer von 42 Tagen durchgeführt, an einer vollständig randomisierten Probe von 10 Hühnern pro Käfig, insgesamt 120 männliche Hühner im Alter von einem Tag des kommerziellen Hybrids Ross 308. Die Datenanalyse wurde unter Verwendung des statistischen SAS-Analyseprogramms vorgenommen, während der Vergleich der Mittelwerte mit dem Duncan multiplen Vergleichstest mit einer Fehlerwahrscheinlichkeit von 5% durchgeführt wurde. Die Ergebnisse zeigten, dass die Zugabe von Vitamin C in einer Menge von 200 mg / kg den Prozentsatz an gesättigten Fettsäuren wie Myristinsäure, Palmitinsäure und Stearinsäure verringert, wobei die Menge an ungesättigten Fettsäuren zunahm und die Menge an gesättigten Fettsäuren abnahm

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Differing Relations to Early Atherosclerosis between Vitamin C from Supplements vs. Food in the Los Angeles Atherosclerosis Study: A Prospective Cohort Study

The Open Cardiovascular Medicine Journal ◽

10.2174/1874192401206010113 ◽

2012 ◽

Vol 6 (1) ◽

pp. 113-121 ◽

Cited By ~ 11

Author(s):

Megha Agarwal ◽

Puja K Mehta ◽

James H Dwyer ◽

Kathleen M Dwyer ◽

Anne M Shircore ◽

...

Keyword(s):

Vitamin C ◽

Serum Cholesterol ◽

Critical Role ◽

Dietary Vitamin ◽

Vitamin Supplement ◽

Carotid Imt ◽

Vitamin C Supplementation ◽

The Impact ◽

Supplement Intake ◽

Early Atherosclerosis

Objective:To determine the relationship of vitamin c intake from supplements vs food on early atherosclerosis detected by carotid intima media thickness (IMT).Background:Oxidative stress and endothelial dysfunction play a critical role in the pathogenesis of atherosclerosis. Dietary vitamin C appears to have anti-oxidant properties and beneficial relations to endothelial function, yet vitamin C taken as a vitamin supplement does not appear to protect from cardiovascular events. The impact of vitamin c intake from supplements vs food on progression of atherosclerosis is unknown.Methods:We examined 3-year progression of carotid IMT in a randomly sampled cohort of 573 healthy women and men aged 40-60 years. Progression of carotid IMT was determined bilaterally with B-mode ultrasound at 3 examinations (1.5-year intervals). Intake of dietary vitamin C was measured by six, 24-hour recall interviews. Intake of vitamin C from vitamin supplements was measured by questionnaire in quartiles of supplement intake and no supplement. Vitamin C wasmeasured in plasma as ascorbic acid.Results:Carotid IMT progressed 10.0±16.5 μm/year (mean±SD) among all those with follow-up (n=500; 87%). For those who took vitamin C supplements, carotid IMT progression increased with dose (p-trend=0.0009). Among persons in the highest quartile (857-5000 mg/day) of vitamin C supplement intake compared to those not consuming any vitamin C supplements, carotid IMT progression increased three-fold (20.3±2.6 versus 7.6±1.8 μm/year (mean±SD); p<0.001). The adverse association of vitamin C supplement intake with carotid IMT was two-fold greater in the upper tertile of serum cholesterol compared to the lower two tertiles (p=0.01). In contrast to the adverse association of vitamin C supplements, vitamin C intake from food had a weak protective relationship on carotid IMT progression (reduced progression -5.0±1.9 μm/year; p=0.008).Conclusions:Vitamin C supplementation is associated with accelerated early atherosclerosis measured by carotid IMT compared to a protective association with vitamin C from food. Theadverse association of vitamin C supplementation may be greater in patients with higher serum cholesterol levels. The current results provide a potential mechanistic understanding for the observed differences between Vitamin C in supplements vs food in prior studies. Given these observations,vitamin C supplementation does not appear to be currently advisable for prevention or treatment of atherosclerosis.

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