scholarly journals The effect of vitamin C on the gene expression profile of sperm protamines in the male partners of couples with recurrent pregnancy loss: A randomized clinical trial

2020 ◽  
Vol 47 (1) ◽  
pp. 68-76
Author(s):  
Saeideh Hamidian ◽  
Ali Reza Talebi ◽  
Farzaneh Fesahat ◽  
Mohammad Bayat ◽  
Ali Mohammad Mirjalili ◽  
...  
Keyword(s):  
Gene Expression ◽  
Vitamin C ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Male Partners ◽  
Expression Levels ◽  
Before And After ◽  
Vitamin C Supplementation

Objective: Since sperm abnormalities are known to be a major reason for recurrent pregnancy loss (RPL), any defects in DNA structure and chromatin condensation can place embryos at risk in the early stage of development and implantation. As antioxidants such as vitamin C may play a protective role against the destruction of protamine genes in sperm chromatin, this study was conducted to evaluate the effects of vitamin C on chromatin and the expression of protamine genes in the male partners of couples with RPL.Methods: Twenty male partners of couples with RPL were selected as the intervention group and received vitamin C supplementation (250 mg daily for 3 months). Healthy fertile men (n=20) were included as controls. Sperm chromatin, DNA integrity, and the expression levels of protamine genes were evaluated before and after treatment.Results: Significant differences were found in sperm morphology, protamine deficiency, and apoptosis between the two groups and before and after vitamin C administration. A significant change was found in mRNA levels of <i>PRM1, PRM2</i>, and the <i>PRM1/PRM2</i> ratio after treatment.Conclusion: Daily oral administration of vitamin C may improve human sperm parameters and DNA integrity by increasing protamine gene expression levels in the male partners of couples with RPL. The beneficial effects of vitamin C supplementation as an antioxidant for the male partners of couples with RPL could lead to improved pregnancy outcomes in these cases.

Effect of High Dose Vitamin C Supplementation on Muscle Soreness, Damage, Function, and Oxidative Stress to Eccentric Exercise

2006 ◽  
Vol 16 (3) ◽  
pp. 270-280 ◽  
Author(s):  
S.C. Bryer ◽  
A.H. Goldfarb
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Eccentric Exercise ◽  
Muscle Function ◽  
Muscle Soreness ◽  
Damage Function ◽  
High Dose ◽  
Before And After ◽  
Vitamin C Supplementation ◽  
Glutathione Oxidation

This study investigated if vitamin C supplementation before and after eccentric exercise could reduce muscle soreness (MS), oxidative stress, and muscle function. Eighteen healthy men randomly assigned to either a placebo (P) or vitamin C (VC) (3 g/d) treatment group took pills for 2 wk prior and 4 d after performing 70 eccentric elbow extensions with their non-dominant arm. MS increased in both groups with significantly reduced MS for the first 24 h with VC. Range of motion was reduced equally in both groups after the exercise (P ≥ 0.05). Muscle force declined equally and was unaffected by treatment. VC attenuated the creatine kinase (CK) increase at 48 h after exercise with similar CK after this time. Gluta-thione ratio (oxidized glutathione/total glutathione) was significantly increased at 4 and 24 h with P but VC prevented this change. These data suggest that vitamin C pretreatment can reduce MS, delay CK increase, and prevent blood glutathione oxidation with little influence on muscle function loss.

scholarly journals Gene Expression Profiling Reveals Progesterone-Mediated Cell Cycle and Immunoregulatory Roles of Hoxa-10 in the Preimplantation Uterus

2003 ◽  
Vol 17 (4) ◽  
pp. 610-627 ◽  
Author(s):  
Mylene W. M. Yao ◽  
Hyunjung Lim ◽  
Daniel J. Schust ◽  
Sung E. Choe ◽  
Anna Farago ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Pregnancy Loss ◽  
Stromal Cell ◽  
Recurrent Pregnancy Loss ◽  
Kinase Inhibitor ◽  
Cyclin Dependent Kinase Inhibitor ◽  
Local Immunosuppression

Abstract Human infertility and recurrent pregnancy loss caused by implantation defects are poorly understood. Hoxa-10-deficient female mice have severe infertility and recurrent pregnancy loss due to defective uterine implantation. Gene expression profiling experiments reveal that Hoxa-10 is an important regulator of two critical events in implantation: stromal cell proliferation and local immunosuppression. At the time of implantation, Hoxa-10 mediates the progesterone-stimulated proliferation of uterine stromal cells. Hoxa-10 mutants express a stromal cell proliferation defect that is accompanied by quantitative or spatial alterations in the expression of two cyclin-dependent kinase inhibitor genes, p57 and p15. Hoxa-10 deficiencyFS also leads to a severe local immunological disturbance, characterized by a polyclonal proliferation of T cells, that occurs in place of the normal progesterone-mediated immunosuppression in the periimplantation uterus.

scholarly journals Increased expression of pentraxin 3 in placental tissues from patients with unexplained recurrent pregnancy loss

2019 ◽  
Vol 22 (1) ◽  
pp. 21-28 ◽  
Author(s):  
S Zeybek ◽  
E Tepeli ◽  
GO Cetin ◽  
V Caner ◽  
H Senol ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Pentraxin 3 ◽  
Specific Expression ◽  
Expression Levels ◽  
Messenger Ribonucleic Acid ◽  
Live Births ◽  
Protein Levels ◽  
Mrna And Protein Expression

AbstractPentraxin 3 (PTX3), a prototypical member of the long pentraxin subfamily, is a evolutionarily conserved multimeric pattern recognition receptor involved in the humoral component of the innate immune system. Pentraxin 3 is released when tissue is stressed or damaged, and interacts with many different ligands. Pentraxin 3 exerts a pivotal role both as a regulator and as an indicator of inflammatory response in the pathogenesis of many diseases such as sepsis, vasculitis and preeclampsia. Uncontrolled inflammatory response is considered a major cause of unexplained recurrent pregnancy loss (URPL). We determined the PTX3 messenger ribonucleic acid (mRNA) and protein expression levels in placentai tissues from 50 women with URPL, and made comparison with those in 50 age-matched control subjects. In quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry analyses, PTX3 mRNA and protein levels, respectively, were significantly increased in URPL patients compared with their respective controls (p = 0.0001). Although no significant correlations were identified between PTX3 expression levels and clinical parameters such as maternal age, numbers of previous pregnancy losses, and gestational age at miscarriage, PTX3 mRNA expression was significantly higher in patients with no live births than in women with previous live births (p = 0.0001). Our study suggests that tissue-specific expression of PTX3 is associated with URPL. Further larger studies are required to determine whether PTX3 expression can be used as a biomarker to manage URPL in routine clinical practice.

scholarly journals Effect of vitamin C supplementation on gingival bleeding

2018 ◽  
Vol 30 (3) ◽  
pp. 178
Author(s):  
Indra Mustika Setia Pribadi ◽  
Nunung Rusminah ◽  
Yanti Rusyanti ◽  
Anne Agustina Suwargiani
Keyword(s):  
Vitamin C ◽  
Chronic Periodontitis ◽  
Host Defence ◽  
Periodontal Pathogens ◽  
Defence Mechanisms ◽  
Periodontal Health ◽  
High Category ◽  
Gingival Bleeding ◽  
Before And After ◽  
Vitamin C Supplementation

Introduction: Periodontitis is a multifactorial disease caused by a disproportion between environmental factors such as periodontal pathogens and the host defence. Genetic factors, hormones, and nutrition may influence host defence mechanisms. One of the nutrition needed for periodontal health is vitamin C. This study was aimed to evaluate the effect of vitamin C supplementation on gingival bleeding. Methods: As much as ninety-eight patients with chronic periodontitis consisted of 42 males and 56 females, aged 30 – 59-years-old participated in this study. Gingival bleeding was assessed before and after 3 months administered with vitamin C supplementation. Gingival bleeding criteria was based on Van der Velden with tooth selection based on Ramfjörd. Result: Gingival bleeding before the administration of vitamin C supplementation was found in the high category at 4 patients (4.1%), medium at 36 patients (36.7%), and low at 58 patients (59.2%).  After 3 months administration of vitamin C supplementation, high category gingival bleeding was found in 2 patients (2%), medium in 16 patients (16.3%), and low in 80 patients (81.6%). Conclusion: There was an effect of vitamin C supplementation on gingival bleeding.Keywords: Chronic periodontitis, vitamin C supplementation, gingival bleeding.

scholarly journals OR18-5 Elevated Semen Oxidative Stress in Male Partners as Novel Marker of Recurrent Pregnancy Loss

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Anastasia Dimakopoulou ◽  
Channa Jayasena ◽  
Utsav Radia ◽  
Monica Figueiredo ◽  
Larisa Franklin Revill ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Male Partners

scholarly journals The effect of grape seed extract and vitamin C feed supplementation on some blood parameters and HSP70 gene expression of broiler chickens suffering from chronic heat stress

2015 ◽  
Vol 14 (3) ◽  
Author(s):  
Hosna Hajati ◽  
Ahmad Hassanabadi ◽  
Abolghasem Golian ◽  
Hassan Nassiri-Moghaddam ◽  
Mohammad Reza Nassiri
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Vitamin C ◽  
Broiler Chickens ◽  
Stress Condition ◽  
Grape Seed ◽  
Blood Parameters ◽  
Feed Supplementation ◽  
Vitamin C Supplementation ◽  
Heat Stress Condition

In this experiment, the effect of hydroalcoholic grape seed extract (GSE) and vitamin C feed supplementation on some blood parameters and heat shock protein 70 (<em>HSP70</em> gene) expression of broiler chickens suffering from chronic heat stress was investigated. Experimental diets included control diet (with no additive), 3 levels of GSE (150, 300, 450 mg/kg), and one level of vitamin C (300 mg/kg). Each diet was fed to 5 replicates of 12 male chicks each, from d 1 to 42. The birds suffered from chronic daily heat stress under 34±1°C temperature with 65 to 70% relative humidity for 5 h from 29 to 42 d of age. Results showed that 300 mg/kg GSE supplementation increased body weight of broilers both before and after heat stress condition (at 28 and 42 d, respectively). Also, birds fed 300 mg GSE/kg diet had higher European production efficiency factor during the whole period of the experiment. Supplementation of GSE decreased the concentration of serum glucose at 28 and 42 d; at 42 d (during heat stress condition) and at 450 mg/kg diet it decreased cholesterol, triglyceride, lowand very low density lipoprotein concentration of serum blood. Vitamin C supplementation decreased serum cholesterol concentration of broilers suffering from heat stress. <em>HSP70</em> gene expression in heart and liver of broilers reduced by GSE and vitamin C supplementation pre- and during chronic heat stress condition.

Downregulation of Circulating miR-23a-3p, miR-101-3p And miR-let-7c In Women With Idiopathic Recurrent Pregnancy Loss

2021 ◽  
Author(s):  
Mina Tutunfroush ◽  
Saeid Ghorbian ◽  
Jafar Mohseni ◽  
Shahla Danaii
Keyword(s):  
Healthy Subjects ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Diagnostic Value ◽  
Clinicopathological Parameters ◽  
Circulating Micrornas ◽  
Expression Levels ◽  
Non Invasive ◽  
Significant Difference ◽  
History Of

Abstract Recently, circulating microRNAs have attracted much attention because they can serve as reliable non-invasive diagnostic and prognostic biomarkers for pregnancy-related complications. So, this study aimed to quantify miR-23a-3p, miR-101-3p and miR-let-7c expression levels in plasma of patients with idiopathic recurrent pregnancy loss (iRPL) and healthy subjects and to evaluate their potential diagnostic value in iRPL patients. A total of 120 plasma samples were obtained from sixty women with a history of at least two consecutive iRPL and sixty healthy women without a history of miscarriage to evaluate the expression levels of the circulating miR-23a-3p, miR-101-3p and miR-let-7c by quantitative real-time polymerase chain reaction (qPCR) technique. The correlation between studied miRNAs and clinicopathological parameters was also assessed. Receiver operating characteristic (ROC) curve was plotted to determine the diagnostic accuracy of miR-23a-3p, miR-101-3p and miR-let-7c in iRPL. Our results showed that the miR-23a-3p expression level in plasma of iRPL patients was lower than those in healthy controls but without a statistically significant difference (P = 0.113). The expression levels of miR-101-3p and miR-let-7c were significantly downregulated in iRPL patients compared with healthy subjects (P < 0.05). The expression levels of miR-23a-3p and miR-let-7c was negatively correlated with number of abortions in iRPL patients. We observed statistically significant positive correlation between miR-23a-3p and miR-101-3p (r = 0.478, P = 0.001), miR-23a-3p and miR-let-7c (r = 0.561, P = 0.0001), miR-101-3p and miR-let-7c (r = 0.533, P = 0.0001) in patients with iRPL. The current study provides evidence indicating that downregulation of miR-23a-3p, miR-101-3p and miR-let-7c may be associated with iRPL.

P–555 Recurrent pregnancy loss is associated with changes in the pre-pregnant endometrial gland transcriptome

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
J Pearson-Farr ◽  
R Lewis ◽  
J Cleal ◽  
Y Cheong
Keyword(s):  
Gene Expression ◽  
Observational Study ◽  
Differential Gene Expression ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Small Sample ◽  
Specific Cell ◽  
Endometrial Gland ◽  
Egg Donor ◽  
Differential Gene

Abstract Study question Do endometrial gland factors influence recurrent pregnancy loss? Summary answer The endometrial gland transcriptome during the window of implantation is altered in women with recurrent pregnancy loss compared to controls. What is known already Secretions from endometrial glands contribute to the uterine environment that supports the attachment and implantation of the embryo in early pregnancy. Studies have attempted to identify an endometrial gene expression pattern associated with recurrent pregnancy loss however, the cellular heterogeneity within the endometrium may obscure important differences in specific cell populations. Study design, size, duration An observational study comparing controls and women with recurrent pregnancy loss. Participants/materials, setting, methods Endometrial samples were collected during the implantation period of the menstrual cycle from five matched participant egg donor controls and women with recurrent pregnancy loss. Endometrial glands were isolated from fresh endometrial biopsies and RNA sequencing was performed. A differential gene expression analysis and a gene ontology enrichment analysis was performed between egg donor controls and women with recurrent pregnancy loss. Main results and the role of chance This study reports a glandular epithelium specific gene expression profile and demonstrates differential gene expression of endometrial glands from women with recurrent pregnancy loss compared to controls. 18 genes were upregulated and 1 gene was downregulated in the endometrial glands from women with recurrent pregnancy loss compared to controls (5% false discovery rate). Biological processes which contain genes that were differentially expressed in women with recurrent pregnancy loss compared to controls include epithelial cell migration and regulation of secretion by the cell. Limitations, reasons for caution This is an observational study with a relatively small sample size. Wider implications of the findings: This study identified differences in gene expression in women with recurrent pregnancy loss that are specifically associated with endometrial glands rather than endometrium as a whole. These differences could be used to identify a perturbed endometrium, isolate causes of recurrent pregnancy loss and develop targeted therapies. Trial registration number Not applicable

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