The Past, Present, and Future of Tissue Regeneration

2013 ◽  
pp. 311-328
Author(s):  
M. Natalia Vergara ◽  
Panagiotis A. Tsonis
Keyword(s):  
Tissue Regeneration ◽  
The Past

scholarly journals A Morphological and Histological Investigation of Imperfect Lungfish Fin Regeneration

2021 ◽  
Vol 9 ◽  
Author(s):  
Vivien Bothe ◽  
Igor Schneider ◽  
Nadia B. Fröbisch
Keyword(s):  
Tissue Regeneration ◽  
Molecular Mechanisms ◽  
Limb Regeneration ◽  
Body Parts ◽  
Serial Sections ◽  
Fin Regeneration ◽  
X Ray ◽  
The Past ◽  
Great Progress ◽  
Made In

Regeneration, the replacement of body parts in a living animal, has excited scientists for centuries and our knowledge of vertebrate appendage regeneration has increased significantly over the past decades. While the ability of amniotes to regenerate body parts is very limited, members of other vertebrate clades have been shown to have rather high regenerative capacities. Among tetrapods (four-limbed vertebrates), only salamanders show unparalleled capacities of epimorphic tissue regeneration including replacement of organ and body parts in an apparently perfect fashion. The closest living relatives of Tetrapoda, the lungfish, show regenerative abilities that are comparable to those of salamanders and recent studies suggest that these high regenerative capacities may indeed be ancestral for bony fish (osteichthyans) including tetrapods. While great progress has been made in recent years in understanding the cellular and molecular mechanisms deployed during appendage regeneration, comparatively few studies have investigated gross morphological and histological features of regenerated fins and limbs. Likewise, rather little is known about how fin regeneration compares morphologically to salamander limb regeneration. In this study, we investigated the morphology and histology of regenerated fins in all three modern lungfish families. Data from histological serial sections, 3D reconstructions, and x-ray microtomography scans were analyzed to assess morphological features, quality and pathologies in lungfish fin regenerates. We found several anomalies resulting from imperfect regeneration in regenerated fins in all investigated lungfish species, including fusion of skeletal elements, additional or fewer elements, and distal branching. The similarity of patterns in regeneration abnormalities compared to salamander limb regeneration lends further support to the hypothesis that high regenerative capacities are plesiomorphic for sarcopterygians.

scholarly journals Bioprinting of Vascularized Tissue Scaffolds: Influence of Biopolymer, Cells, Growth Factors, and Gene Delivery

2019 ◽  
Vol 2019 ◽  
pp. 1-20 ◽  
Author(s):  
M. D. Sarker ◽  
Saman Naghieh ◽  
N. K. Sharma ◽  
Liqun Ning ◽  
Xiongbiao Chen
Keyword(s):  
Growth Factors ◽  
Gene Delivery ◽  
Tissue Regeneration ◽  
Research Direction ◽  
Future Research ◽  
Biological Factors ◽  
The Past ◽  
Organ Regeneration ◽  
Recent Advancement ◽  
Biological Performance

Over the past decades, tissue regeneration with scaffolds has achieved significant progress that would eventually be able to solve the worldwide crisis of tissue and organ regeneration. While the recent advancement in additive manufacturing technique has facilitated the biofabrication of scaffolds mimicking the host tissue, thick tissue regeneration remains challenging to date due to the growing complexity of interconnected, stable, and functional vascular network within the scaffold. Since the biological performance of scaffolds affects the blood vessel regeneration process, perfect selection and manipulation of biological factors (i.e., biopolymers, cells, growth factors, and gene delivery) are required to grow capillary and macro blood vessels. Therefore, in this study, a brief review has been presented regarding the recent progress in vasculature formation using single, dual, or multiple biological factors. Besides, a number of ways have been presented to incorporate these factors into scaffolds. The merits and shortcomings associated with the application of each factor have been highlighted, and future research direction has been suggested.

scholarly journals Antimicrobial Films based on Chitosan, Collagen, and ZnO for Skin Tissue Regeneration

2020 ◽  
Vol 11 (4) ◽  
pp. 11985-11995
Keyword(s):  
Zinc Oxide ◽  
Tissue Regeneration ◽  
Wound Dressing ◽  
Bacterial Infections ◽  
Cell Activity ◽  
The Past ◽  
Major Interest ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Bacterial infections represent a health issue worldwide. Over the past years, major interest has been given to developing new antibacterial and regenerative materials due to the increasing number of infections with pathogenic strains and the alarming antibiotic resistance. Polymer films and membranes with protective or even anti-infectious activity were developed. Some of them were based on nanoparticles with the main advantage that the resistance's development only seldom appears. Considering the Collagenic nature of the skin and the beneficial properties of Chitosan, the two polymers were proposed to be used in developing nanostructured wound dressing loaded with ZnO nanoparticles. These nanostructured materials confer promising characteristics to be used as anti-infectious wound dressing being biocompatible, antimicrobial against C. albicans and S. aureus, and highly hydrophilic able to absorb over 2300% water, which confer the premises of maintaining proper humidity and exudate absorption during wound healing. Fibrillar structures with Chitosan, Collagen, and Zinc Oxide can be an alternative for tissue regeneration. Electrospinning was used to fabricate fibrillar structures consisting of doing Chitosan, Collagen, and Zinc Oxide. The Zinc Oxide was used to defend the wound against infections and the beneficial role of Zn2+ in enhancing cell activity. The morphology of the fibrillar structures was studied by scanning electron microscopy while Collagen integrity by FT-IR spectroscopy.

scholarly journals Past, Present, and Future of Regeneration Therapy in Oral and Periodontal Tissue: A Review

2019 ◽  
Vol 9 (6) ◽  
pp. 1046 ◽  
Author(s):  
Hwa-Sun Lee ◽  
Soo-Hwan Byun ◽  
Seoung-Won Cho ◽  
Byoung-Eun Yang
Keyword(s):  
Tissue Regeneration ◽  
Computer Aided Design ◽  
Alveolar Bone ◽  
Three Dimensional ◽  
Common Disease ◽  
Tissue Destruction ◽  
Functional Layer ◽  
The Past ◽  
Space Maintenance ◽  
Cad Cam

Chronic periodontitis is the most common disease which induces oral tissue destruction. The goal of periodontal treatment is to reduce inflammation and regenerate the defects. As the structure of periodontium is composed of four types of different tissue (cementum, alveolar bone periodontal ligament, and gingiva), the regeneration should allow different cell proliferation in the separated spaces. Guided tissue regeneration (GTR) and guided bone regeneration (GBR) were introduced to prevent epithelial growth into the alveolar bone space. In the past, non-absorbable membranes with basic functions such as space maintenance were used with bone graft materials. Due to several limitations of the non-absorbable membranes, membranes of the second and third generation equipped with controlled absorbability, and a functional layer releasing growth factors or antimicrobials were introduced. Moreover, tissue engineering using biomaterials enabled faster and more stable tissue regeneration. The scaffold with three-dimensional structures manufactured by computer-aided design and manufacturing (CAD/CAM) showed high biocompatibility, and promoted cell infiltration and revascularization. In the future, using the cell sheath, pre-vascularizing and bioprinting techniques will be applied to the membrane to mimic the original tissue itself. The aim of the review was not only to understand the past and the present trends of GTR and GBR, but also to be used as a guide for a proper future of regeneration therapy in the oral region.

scholarly journals Digit Tip Injuries: Current Treatment and Future Regenerative Paradigms

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Travis J. Miller ◽  
Peter L. Deptula ◽  
Gregory M. Buncke ◽  
Zeshaan N. Maan
Keyword(s):  
Clinical Practice ◽  
Regenerative Medicine ◽  
Tissue Regeneration ◽  
Lower Order ◽  
Life Forms ◽  
Current Treatment ◽  
Model Organisms ◽  
Regenerative Capacity ◽  
The Past ◽  
Ability To Regenerate

Over the past several decades there has been a profound increase in the understanding of tissue regeneration, driven largely by the observance of the tremendous regenerative capacity in lower order life forms, such as hydra and urodeles. However, it is known that humans and other mammals retain the ability to regenerate the distal phalanges of the digits after amputation. Despite the increased knowledge base on model organisms regarding regenerative paradigms, there is a lack of application of regenerative medicine techniques in clinical practice in regard to digit tip injury. Here, we review the current understanding of digit tip regeneration and discuss gaps that remain in translating regenerative medicine into clinical treatment of digit amputation.

scholarly journals Transdifferentiation Meets Next-generation Biotechnologies

StemJournal ◽  
2022 ◽  
pp. 1-11
Author(s):  
Xiaoshan Ke ◽  
Abhimanyu Thakur ◽  
Huanhuan Joyce Chen
Keyword(s):  
Tissue Regeneration ◽  
Disease Modeling ◽  
Directed Differentiation ◽  
Next Generation ◽  
Cell Type ◽  
Promising Alternative ◽  
Cell Lineages ◽  
The Past ◽  
Differentiated Cells ◽  
Cell Sources

Transdifferentiation is the process of converting terminally differentiated cells to another cell type. Being less time-consuming and free from tumorigenesis, it is a promising alternative to directed differentiation, which provides cell sources for tissue regeneration therapy and disease modeling. In the past decades, transdifferentiation was found to happen within or across the cell lineages, being induced by overexpression of key transcription factors, chemical cocktail treatments, etc. Implementing next-generation biotechnologies, such as genome editing tools and scRNA-seq, improves current protocols and has the potential to facilitate discovery in new pathways of transdifferentiation, which will accelerate its application in clinical use.

scholarly journals Cytonemes, Their Formation, Regulation, and Roles in Signaling and Communication in Tumorigenesis

2019 ◽  
Vol 20 (22) ◽  
pp. 5641 ◽  
Author(s):  
Casas-Tintó ◽  
Portela
Keyword(s):  
Cell Signaling ◽  
Cancer Progression ◽  
Tissue Regeneration ◽  
Cell Types ◽  
Tissue Homeostasis ◽  
Adult Tissue ◽  
The Past ◽  
Signaling Mechanisms ◽  
Brain Tumor Cells ◽  
Membrane Protrusions

Increasing evidence during the past two decades shows that cells interconnect and communicate through cytonemes. These cytoskeleton-driven extensions of specialized membrane territories are involved in cell–cell signaling in development, patterning, and differentiation, but also in the maintenance of adult tissue homeostasis, tissue regeneration, and cancer. Brain tumor cells in glioblastoma extend ultralong membrane protrusions (named tumor microtubes, TMs), which contribute to invasion, proliferation, radioresistance, and tumor progression. Here we review the mechanisms underlying cytoneme formation, regulation, and their roles in cell signaling and communication in epithelial cells and other cell types. Furthermore, we discuss the recent discovery of glial cytonemes in the Drosophila glial cells that alter Wingless (Wg)/Frizzled (Fz) signaling between glia and neurons. Research on cytoneme formation, maintenance, and cell signaling mechanisms will help to better understand not only physiological developmental processes and tissue homeostasis but also cancer progression.

Conventional Electrospinning vs. Emulsion Electrospinning: A Comparative Study on the Development of Nanofibrous Drug/Biomolecule Delivery Vehicles

2011 ◽  
Vol 410 ◽  
pp. 118-121 ◽  
Author(s):  
Chong Wang ◽  
Sze Nga Tong ◽  
Yuk Hang Tse ◽  
Min Wang
Keyword(s):  
Tissue Regeneration ◽  
Glycolic Acid ◽  
Fibrous Tissue ◽  
Tissue Engineering Scaffolds ◽  
The Past ◽  
Model Protein ◽  
Delivery Vehicles ◽  
Direct Dissolution ◽  
Biological Performance

Over the past decade, intensive research has been conducted on electrospinning of fibrous tissue engineering scaffolds and their applications in body tissue regeneration. For providing multifunctions and/or enhancing the biological performance, drugs or biomolecules can be incorporated in electrospun fibers using normally one of these techniques: (1) direct dissolution, (3) emulsion electrospinning, and (3) coaxial electrospinning. In this investigation, for constructing nanofibrous delivery vehicles, conventional electrospinning using polymer solutions with directly dissolved drugs or biomolecules and emulsion electrospinning were studied and compared. Bovine serum albumin (BSA) was used as a model protein and the drug was rifamycin, a hydrophobic antibiotic. A poly (lactic-co-glycolic acid) containing the protein or drug was electrospun into fibers. In these two routes of fabricating drug-or biomolecule-loaded nanofibers, different polymer concentrations and emulsion formulations were investigated. Various aspects of the fibrous delivery vehicles were investigated using several techniques and the in vitro release behaviour was studied.

scholarly journals Evolution of Thoracic Surgery in Canada

2015 ◽  
Vol 22 (2) ◽  
pp. e8-e14
Author(s):  
Jean Deslauriers ◽  
F Griffith Pearson ◽  
Bill Nelems
Keyword(s):  
Intensive Care ◽  
Thoracic Surgery ◽  
Lung Transplantation ◽  
Tissue Regeneration ◽  
Organ Preservation ◽  
Ex Vivo ◽  
The Past ◽  
Canadian Experience ◽  
Local Practices ◽  
Material Form

BACKGROUND: Canada’s contributions toward the 21st century’s practice of thoracic surgery have been both unique and multilayered. Scattered throughout are tales of pioneers where none had gone before, where opportunities were greeted by creativity and where iconic figures followed one another.OBJECTIVE: To describe the numerous and important achievements of Canadian thoracic surgeons in the areas of surgery for pulmonary tuberculosis, thoracic oncology, airway surgery and lung transplantation.METHOD: Information was collected through reading of the numerous publications written by Canadian thoracic surgeons over the past 100 years, interviews with interested people from all thoracic surgery divisions across Canada and review of pertinent material form the archives of several Canadian hospitals and universities.RESULTS: Many of the developments occurred by chance. It was the early and specific focus on thoracic surgery, to the exclusion of cardiac and general surgery, that distinguishes the Canadian experience, a model that is now emerging everywhere. From lung transplantation in chimera twin calves to ex vivo organ preservation, from the removal of airways to tissue regeneration, and from intensive care research to complex science, Canadians have excelled in their commitment to research. Over the years, the influence of Canadian thoracic surgery on international practice has been significant.CONCLUSIONS: Canada spearheaded the development of thoracic surgery over the past 100 years to a greater degree than any other country. From research to education, from national infrastructures to the regionalization of local practices, it happened in Canada.

scholarly journals Hepatocyte generation in liver homeostasis, repair, and regeneration

2022 ◽  
Vol 11 (1) ◽  
Author(s):  
Wenjuan Pu ◽  
Bin Zhou
Keyword(s):  
Cell Fate ◽  
Tissue Regeneration ◽  
Cell Lineage ◽  
Lineage Tracing ◽  
Cellular Reprogramming ◽  
Hepatocyte Proliferation ◽  
Genetic Lineage ◽  
Regenerative Capacity ◽  
The Past ◽  
Genetic Lineage Tracing

AbstractThe liver has remarkable capability to regenerate, employing mechanism to ensure the stable liver-to-bodyweight ratio for body homeostasis. The source of this regenerative capacity has received great attention over the past decade yet still remained controversial currently. Deciphering the sources for hepatocytes provides the basis for understanding tissue regeneration and repair, and also illustrates new potential therapeutic targets for treating liver diseases. In this review, we describe recent advances in genetic lineage tracing studies over liver stem cells, hepatocyte proliferation, and cell lineage conversions or cellular reprogramming. This review will also evaluate the technical strengths and limitations of methods used for studies on hepatocyte generation and cell fate plasticity in liver homeostasis, repair and regeneration.

Sign in / Sign up

Export Citation Format

Share Document