Experimental and Modeling Analysis of the Cell-Wall Fracture of Nannochloropsis Oculata

2020 ◽  
Vol 36 (6) ◽  
pp. 789-797
Author(s):  
Wei-Hsuan Hsu ◽  
Yin-Hsuan Chien ◽  
Hung-Yin Tsai

ABSTRACTIn this study, a spherical indenter mounted on an atomic force microscope (AFM) was used to compress a Nannochloropsis oculata (N. oculata) cell on a poly-l-lysine coated slide. A mathematical model of the cell, which was derived by considering a fluid-filled spherical shell with axisymmetric compression between a sphere and an infinite flat plate, is proposed. In the construction of this mathematical model, the spherical shell was assumed to be a homogenous, isotropic, and elastic material. Thin-film theory was applicable to the spherical shell because the thickness of the shell was nearly negligible compared with its diameter. The governing equations of the contact and noncontact regions were converted from a boundary condition problem to an initial value problem. Then, the fourth-order Runge–Kutta method was applied to solve the transformed governing equations. The force curve obtained from the compression experiment was compared with the theoretical results derived from the proposed model. Furthermore, the numerical solution of the proposed model was verified to be consistent with the experimental data. The mechanical properties of cell walls were confirmed by applying the least square error method. Subsequently, the contact radius, inner pressure and tension distribution of the cell wall could be determined using the proposed model. The models proposed in other studies are suitable for analyzing the compression characteristics of cells whose size is of the order of tens of micrometers and millimeters. By contrast, the model proposed in this study can analyze the compression characteristics of N. oculata, which is only a few micrometers in diameter. Furthermore, a force curve that accurately describes the deformation behavior of N. oculata under strain levels of 25% was established.

Author(s):  
Kenyu Uehara ◽  
Takashi Saito

Abstract We have modeled dynamics of EEG with one degree of freedom nonlinear oscillator and examined the relationship between mental state of humans and model parameters simulating behavior of EEG. At the IMECE conference last year, Our analysis method identified model parameters sequentially so as to match the waveform of experimental EEG data of the alpha band using one second running window. Results of temporal variation of model parameters suggested that the mental condition such as degree of concentration could be directly observed from the dynamics of EEG signal. The method of identifying the model parameters in accordance with the EEG waveform is effective in examining the dynamics of EEG strictly, but it is not suitable for practical use because the analysis (parameter identification) takes a long time. Therefore, the purpose of this study is to test the proposed model-based analysis method for general application as a neurotechnology. The mathematical model used in neuroscience was improved for practical use, and the test was conducted with the cooperation of four subjects. model parameters were experimentally identified approximately every one second by using least square method. We solved a binary classification problem of model parameters using Support Vector Machine. Results show that our proposed model-based EEG analysis is able to discriminate concentration states in various tasks with an accuracy of over 80%.


Author(s):  
Olga Mikhaylovna Tikhonova ◽  
Alexander Fedorovich Rezchikov ◽  
Vladimir Andreevich Ivashchenko ◽  
Vadim Alekseevich Kushnikov

The paper presents the system of predicting the indicators of accreditation of technical universities based on J. Forrester mechanism of system dynamics. According to analysis of cause-and-effect relationships between selected variables of the system (indicators of accreditation of the university) there was built the oriented graph. The complex of mathematical models developed to control the quality of training engineers in Russian higher educational institutions is based on this graph. The article presents an algorithm for constructing a model using one of the simulated variables as an example. The model is a system of non-linear differential equations, the modelling characteristics of the educational process being determined according to the solution of this system. The proposed algorithm for calculating these indicators is based on the system dynamics model and the regression model. The mathematical model is constructed on the basis of the model of system dynamics, which is further tested for compliance with real data using the regression model. The regression model is built on the available statistical data accumulated during the period of the university's work. The proposed approach is aimed at solving complex problems of managing the educational process in universities. The structure of the proposed model repeats the structure of cause-effect relationships in the system, and also provides the person responsible for managing quality control with the ability to quickly and adequately assess the performance of the system.


1986 ◽  
Vol 18 (7-8) ◽  
pp. 239-248 ◽  
Author(s):  
Sung Ryong Ha ◽  
Dwang Ho Lee ◽  
Sang Eun Lee

Laboratory scale experiments were conducted to develop a mathematical model for the anaerobic digestion of a mixture of night soil and septic tank sludge. The optimum mixing ratio by volume between night soil and septic tank sludge was found to be 7:3. Due to the high solids content in the influent waste, mixed-liquor volatile suspended solids (MLVSS) was not considered to be a proper parameter for biomass concentration, therefore, the active biomass concentration was estimated based on deoxyribonucleic acid (DNA) concentration in the reactor. The weight ratio between acidogenic bacteria and methanogenic bacteria in the mixed culture of a well-operated anaerobic digester was approximately 3:2. The proposed model indicates that the amount of volatile acid produced and the gas production rate can be expressed as a function of hydraulic residence time (HRT). The kinetic constants of the two phases of the anaerobic digestion process were determined, and a computer was used to simulate results using the proposed model for the various operating parameters, such as BOD5 and volatile acid concentrations in effluent, biomass concentrations and gas production rates. These were consistent with the experimental data.


2021 ◽  
Vol 9 (2) ◽  
pp. 118
Author(s):  
Xinqing Zhuang ◽  
Keliang Yan ◽  
Pan Gao ◽  
Yihua Liu

Anchor dragging is a major threat to the structural integrity of submarine pipelines. A mathematical model in which the mechanical model of chain and the bearing model of anchor were coupled together. Based on the associated flow rule, an incremental procedure was proposed to solve the spatial state of anchor until it reaches the ultimate embedding depth. With an indirect measurement method for the anchor trajectory, a model test system was established. The mathematical model was validated against some model tests, and the effects of two parameters were studied. It was found that both the ultimate embedding depth of a dragging anchor and the distance it takes to reach the ultimate depth increase with the shank-fluke pivot angle, but decrease as the undrained shear strength of clay increases. The proposed model is supposed to be useful for the embedding depth calculation and guiding the design of the pipeline burial depth.


Electronics ◽  
2021 ◽  
Vol 10 (15) ◽  
pp. 1843
Author(s):  
Jelena Vlaović ◽  
Snježana Rimac-Drlje ◽  
Drago Žagar

A standard called MPEG Dynamic Adaptive Streaming over HTTP (MPEG DASH) ensures the interoperability between different streaming services and the highest possible video quality in changing network conditions. The solutions described in the available literature that focus on video segmentation are mostly proprietary, use a high amount of computational power, lack the methodology, model notation, information needed for reproduction, or do not consider the spatial and temporal activity of video sequences. This paper presents a new model for selecting optimal parameters and number of representations for video encoding and segmentation, based on a measure of the spatial and temporal activity of the video content. The model was developed for the H.264 encoder, using Structural Similarity Index Measure (SSIM) objective metrics as well as Spatial Information (SI) and Temporal Information (TI) as measures of video spatial and temporal activity. The methodology that we used to develop the mathematical model is also presented in detail so that it can be applied to adapt the mathematical model to another type of an encoder or a set of encoding parameters. The efficiency of the segmentation made by the proposed model was tested using the Basic Adaptation algorithm (BAA) and Segment Aware Rate Adaptation (SARA) algorithm as well as two different network scenarios. In comparison to the segmentation available in the relevant literature, the segmentation based on the proposed model obtains better SSIM values in 92% of cases and subjective testing showed that it achieves better results in 83.3% of cases.


Water ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 1888
Author(s):  
Óscar E. Coronado-Hernández ◽  
Ivan Derpich ◽  
Vicente S. Fuertes-Miquel ◽  
Jairo R. Coronado-Hernández ◽  
Gustavo Gatica

The study of draining processes without admitting air has been conducted using only steady friction formulations in the implementation of governing equations. However, this hydraulic event involves transitions from laminar to turbulent flow, and vice versa, because of the changes in water velocity. In this sense, this research improves the current mathematical model considering unsteady friction models. An experimental facility composed by a 4.36 m long methacrylate pipe was configured, and measurements of air pocket pressure oscillations were recorded. The mathematical model was performed using steady and unsteady friction models. Comparisons between measured and computed air pocket pressure patterns indicated that unsteady friction models slightly improve the results compared to steady friction models.


Author(s):  
Xuan Li ◽  
Bingkui Chen ◽  
Yawen Wang ◽  
Guohua Sun ◽  
Teik C. Lim

In this paper, the planar double-enveloping method is presented for the generation of tooth profiles of the internal gear pair for various applications, such as gerotors and gear reducers. The main characteristic of this method is the existence of double contact between one tooth pair such that the sealing property, the load capacity and the transmission precision can be significantly improved as compared to the conventional configuration by the single-enveloping theory. Firstly, the generation principle of the planar double-enveloping method is introduced. Based on the coordinate transformation and the envelope theory, the general mathematical model of the double-enveloping internal gear pair is presented. By using this model, users can directly design different geometrical shape profiles to obtain a double-enveloping internal gear pair with better meshing characteristics. Secondly, to validate the effectiveness of the proposed model, specific mathematical formulations of three double-enveloping internal gear pairs which apply circular, parabolic and elliptical curves as the generating curves are given. The equations of tooth profiles and meshing are derived and the composition of tooth profiles is analyzed. Finally, numerical examples are provided for an illustration.


2016 ◽  
Vol 10 (10) ◽  
pp. 133
Author(s):  
Mohammad Ali Nasiri Khalili ◽  
Mostafa Kafaei Razavi ◽  
Morteza Kafaee Razavi

Items supplies planning of a logistic system is one of the major issue in operations research. In this article the aim is to determine how much of each item per month from each supplier logistics system requirements must be provided. To do this, a novel multi objective mixed integer programming mathematical model is offered for the first time. Since in logistics system, delivery on time is very important, the first objective is minimization of time in delivery on time costs (including lack and maintenance costs) and the cost of purchasing logistics system. The second objective function is minimization of the transportation supplier costs. Solving the mathematical model shows how to use the Multiple Objective Decision Making (MODM) can provide the ensuring policy and transportation logistics needed items. This model is solved with CPLEX and computational results show the effectiveness of the proposed model.


2013 ◽  
Vol 655-657 ◽  
pp. 2262-2265
Author(s):  
Jian Guo Kong

Air traffic flow management is the key to evaluate airspace capacity reasonably and accurately. Based on the flight features of terminal route intersection, this paper builds a mathematical model for scattered flight of departure aircraft, and then evaluates the terminal capacity based on this model. By combining data from Flight Data Recorder (FDR) and flight schedule with the model, an example-runway 02R of Guangzhou Baiyun airport terminal was given to show the effectiveness of the proposed model.


2013 ◽  
Vol 805-806 ◽  
pp. 716-720
Author(s):  
Tao Xu ◽  
Tian Long Shao ◽  
Dong Fang Zhang

Combined with the contents of the study-PSS low-pass link parameter identification. Least-squares method is selected. Using least-square method for PSS low-pass link mathematical model are also deduced. For the results, because of the mathematical model is solving nonlinear equations, cannot used by the Newton method directly. So we choose to use Newton iterations, with this feature, choose to use MATLAB software to solve the equation. Identification of the use of MATLAB software lags after the PSS parameters obtained recognition results compared with national standards, identifying and verifying the practicability.


Sign in / Sign up

Export Citation Format

Share Document