Effect of increased levels of dietary alpha-linolenic acid on the n-3 polyunsaturated fatty acid bioavailability and oxidative stress in rat

2021 ◽  
pp. 1-43
Author(s):  
Leslie Couëdelo ◽  
Benjamin Buaud ◽  
Hélène Abrous ◽  
Ikram Chamekh-Coelho ◽  
Didier Majou ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Fatty Acids ◽  
Linolenic Acid ◽  
Unsaturated Fatty Acids ◽  
Dietary Lipids ◽  
Alpha Linolenic Acid ◽  
Lipid Fractions ◽  
The Impact ◽  
And Oxidative Stress

Abstract We investigated the impact of increased alpha-linolenic acid (ALA) dietary levels on its plasma bioavailability and its bioconversion in n-3 long chain poly unsaturated fatty acids (LC-PUFA) during a 60-day kinetics and the oxidative stress potentially associated. Rats were submitted to a normolipidic diet providing 0, 3, 10 and 24% ALA of dietary lipids during a kinetics of 0, 15, 30 and 60 days. The lipid peroxidation and oxidative stress (nitric oxide (NO) contents and catalase (CAT), superoxide dismutase (SOD), gluthation peroxidase (GPx) activities) were studied in the liver and plasma. Data demonstrated that when the diet was deprived in n-3 PUFAs, ALA, (eicosanoic acid) EPA and docosahexaenoic acid (DHA) levels decreased in all lipid fractions of plasma and in red blood cell (RBC) lipids. However when ALA was added in the diet, its bioavailability and its bioconversion in EPA was linearly correlated with the ALA intake (R2=0.98). When the diet provided 10 to 24% ALA in dietary lipids (LA/ALA, 1.6 and 5.5 respectively), ALA and EPA were more broadly packaged in all lipid fractions (triglyceride (TG), cholesterol ester (CE) and free fatty acids (FFA)) of plasma from 15 to 30 days timeframe. However only 3% ALA in the lipid diet was sufficient to promote the maximal bioconversion of ALA in DHA in phospholipid (PL) and TG fractions. Additionally, the improvement of ALA bioconversion in EPA and DHA did not impact the oxidative stress markers wich can limit lipid peroxidation. To conclude, this study demonstrated that 10% ALA in the diet for 15-30 days is the gold diet to promote its bioavailability and its bioconversion in n-3 PUFAs in rat and allowed the greatest levels in plasma and RBCs.

scholarly journals Hyperglycemia and Hyperlipidemia Induced Inflammation and Oxidative Stress in Human T Lymphocytes and Salutary Effects of ω- 3 Fatty Acid.

2020 ◽  
pp. 1-9
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Fatty Acids ◽  
T Cells ◽  
Fatty Acid ◽  
T Lymphocytes ◽  
Palmitic Acid ◽  
Linolenic Acid ◽  
High Glucose ◽  
Human T Lymphocytes

Abstract Type 2 Diabetes conditions are associated with hyperglycemia and hyperlipidemia; however, the role of Saturated Fatty Acids (SFA) vs. Unsaturated Fatty Acids (UFA) and high glucose on human T lymphocytes (T cells) is not known. We investigated the salutary effect of the UFA ω-3 fatty acid, α- linolenic acid, on glucose and SFA, palmitic acid, induced activation on T cells as a cause of the inflammatory process with high glucose and SFA foods. These cells in the presence of palmitic acid and/or high glucose but not linolenic acid exhibited a concentration and time-dependent emergence of insulin receptors (INSR), expression, generation of ROS, lipid peroxidation, cytokines and NF-kB p65 translocation to the nucleus. Whereas, activation of the cells by elevated levels of glucose and palmitic acid were additive, addition of linolenic acid in a dose-related manner inhibited activation of cells by glucose and palmitic acid and reduced markers of oxidative stress, lipid peroxidation and cytokines. We propose that UFAs such as α-linolenic acid may serve as a protective mechanism against the deleterious effects of hyperglycemia and hyperlipidemia of high sugar and SFA foods as in diabetes.

scholarly journals Identification and Analysis of the FAD Gene Family in Walnuts (Juglans regia L.) Based on Transcriptome Data

2020 ◽  
Author(s):  
Kai Liu ◽  
Shugang Zhao ◽  
Shuang Wang ◽  
Hongxia Wang ◽  
Zhihua Zhang
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Gene Family ◽  
Linolenic Acid ◽  
Betula Pendula ◽  
Significant Positive Correlation ◽  
Unsaturated Fatty Acids ◽  
Acid Synthesis ◽  
Transcriptome Data ◽  
Alpha Linolenic Acid

Abstract Background: Walnut fatty acids, the main component of walnut kernels, contain a large amount of unsaturated fatty acids, such as linoleic acid and linolenic acid, which are essential fatty acids in humans and have important effects on human growth and health. Fatty acid desaturase (FAD) is widely distributed throughout the biological world. Its main function is to remove hydrogen from carbon chains in the biosynthesis of unsaturated fatty acids to synthesize C=C double bonds. Results: In the current research, 24 members of the JrFAD gene family were identified by bioinformatics analysis; the expression of fatty acid synthesis genes in walnut kernels at different developmental stages was analysed by transcriptome sequencing, and the expression of JrFAD3-1 , an enzyme gene for linolenic acid synthesis, was particularly prominent. The results showed that the relative expression level of FAD3-1 changed dramatically with the kernel development stage, and the expression changes showed a "bell shape". There was a significant positive correlation between the expression of JrFAD3-1 from 90-100 DAF (days after flower) and the content of alpha-linolenic acid from 100-130 DAF, with a correlation coefficient of 0.991. JrFAD3-1 can be considered closely related to Betula pendula and Corylus heterophylla . Conclusion: 24 members of the JrFAD gene family consist of four subfamilies: the SAD desaturase subfamily, Δ7/Δ9 desaturase subfamily, Δ12/ω-3 desaturase subfamily and "front-end" desaturase subfamily. A key gene Jr FAD3-1 was obtained based on transcriptome data and there was a significant positive correlation between the expression of JrFAD3-1 from 90-100 DAF and the content of alpha-linolenic acid from 100-130 DAF. JrFAD3-1 can be considered closely related to Betula pendula and Corylus heterophylla .

Using nutritional and oxidative stress to increase content of healthbeneficial fatty acids in oleaginous and non-oleaginous yeasts

2016 ◽  
Vol 70 (10) ◽  
Author(s):  
Karolína Pádrová ◽  
Irena Kolouchová ◽  
Tomáš Řezanka ◽  
Alena Čejková
Keyword(s):  
Oxidative Stress ◽  
Fatty Acids ◽  
Lipid Content ◽  
Unsaturated Fatty Acids ◽  
Yeast Species ◽  
Marine Organisms ◽  
Stress Conditions ◽  
Oleaginous Yeasts ◽  
Biotechnological Production ◽  
And Oxidative Stress

AbstractYeast responses to stress conditions include an increase in lipid content and concomitant changes in content of saturated and unsaturated fatty acids. Some fatty acids are among the dietetically important fatty acids and new possibilities are sought for their biotechnological production in addition to those already exploited from marine organisms, nuts and other sources. The possibility of the production of palmitoleic and linoleic acids resulting from new approaches to traditional biotechnologically useful yeast species (

scholarly journals Decrease of Atherosclerosis Markers and Oxidative Stress After 1 Year Administration of Omega-3 Fatty Acids Supplements in Patients with Metabolic Syndrome

2014 ◽  
Vol 21 (3) ◽  
pp. 175-183
Author(s):  
Andreea Dragomir ◽  
Emila Rusu ◽  
Mihaela Posea ◽  
Gabriela Radulian
Keyword(s):  
Oxidative Stress ◽  
Metabolic Syndrome ◽  
Fatty Acids ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Atherosclerosis Progression ◽  
Group A ◽  
Group B ◽  
The Impact ◽  
And Oxidative Stress

AbstractBackground and Aims: To assess the impact of 1 year administration of omega-3 fatty acids supplements on oxidative stress parameters and atherosclerosis progression. Material and Methods: A total of 284 patients with metabolic syndrome, aged 61±6.7 years, without clinical evidence of atherosclerosis were allocated to 2 groups, matched by sex and age: group A (140 patients) - diet according to ESC/EASD recommendations; group B (144 patients) - the same diet + capsules of fish oil (1g eicosapentanoic acid, 1g docosahexanoic acid, 0,1g a-tocopherol acetate). Body fat (BF) was measured by bioimpedance analysis. For oxidative stress evaluation we used the FormOx systems monitor on a blood drop and for progression of atherosclerosis the intima-media thickness (IMT) at common carotid artery. Patients were evaluated at baseline, after 6 months and 1 year. Results: IMT significantly decreased in group B vs group A at 1 year (p<0.0001) and was correlated with %BF (p<0.001), waist-to-hip ratio (WHR) (p=0.002), leptin (p< 0.001), adiponectin (p<0.05), leptin/adiponectin ratio (p< 0.001) and oxidative stress (p<0.001). Conclusions: One year administrations of omega-3 PUFA enriched diet reduces cardiovascular risk of metabolic syndrome patients, resulting in a significant decrease of oxidative stress and atherosclerosis progression.

scholarly journals Sea Buckthorn (Hippophae Rhamnoides) Lipids and their Functionality on Health Aspects

2016 ◽  
Vol 4 (3) ◽  
pp. 182-194 ◽  
Author(s):  
George Zakynthinos ◽  
Theodoros Varzakas ◽  
Dimitrios Petsios
Keyword(s):  
Oxidative Stress ◽  
Fatty Acids ◽  
Cardiovascular Health ◽  
Unsaturated Fatty Acids ◽  
Natural Antioxidants ◽  
Sea Buckthorn ◽  
Glucose Response ◽  
Beneficial Effects ◽  
Orange Yellow ◽  
And Oxidative Stress

Hippophae rhamnoides, also named as Sea Buckthorn(SB) is a spiny bush with long and narrow leaves, and orange yellow berries. It is native to Europe and mainly to Asia. The leaves, fruits and the seeds of sea bucHippophae rhamnoides, also named as Sea Buckthorn(SB) is a spiny bush with long and narrow leaves, and orange yellow berries. It is native to Europe and mainly to Asia. The leaves, fruits and the seeds of sea buckthorn contain many bioactive compounds. These compounds are various natural antioxidants such as ascorbic acid, tocopherols, carotenoids, flavonoids, whereas besides them all we can find macronutrients such as proteins, lipids (mainly unsaturated fatty acids and phytosterols), vitamins (especially vitamin C), minerals, small amounts of sugars and organic acids . The present review focuses on the functionality of SB lipids and refers to those studies that suggest the sea buckthorn could have various beneficial effects on cardiovascular health, insulin and glucose response, renal health, hepatic health, Gastric health, epidermis health, cancer, ophthalmologic health and oxidative stress and toxicity.kthorn contain many bioactive compounds. These compounds are various natural antioxidants such as ascorbic acid, tocopherols, carotenoids, flavonoids, whereas besides them all we can find macronutrients such as proteins, lipids (mainly unsaturated fatty acids and phytosterols), vitamins (especially vitamin C), minerals, small amounts of sugars and organic acids . The present review focuses on the functionality of SB lipids and refers to those studies that suggest the sea buckthorn could have various beneficial effects on cardiovascular health, insulin and glucose response, renal health, hepatic health, Gastric health, epidermis health, cancer, ophthalmologic health and oxidative stress and toxicity.

scholarly journals Effects of a Fish Oil Rich in Docosahexaenoic Acid on Cardiometabolic Risk Factors and Oxidative Stress in Healthy Rats

Marine Drugs ◽  
2021 ◽  
Vol 19 (10) ◽  
pp. 555
Author(s):  
Bernat Miralles-Pérez ◽  
Lucía Méndez ◽  
Maria Rosa Nogués ◽  
Vanessa Sánchez-Martos ◽  
Àngels Fortuño-Mar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Risk Factors ◽  
Lipid Peroxidation ◽  
Fatty Acids ◽  
Docosahexaenoic Acid ◽  
Fish Oil ◽  
Soybean Oil ◽  
Cardiometabolic Risk ◽  
Coconut Oil ◽  
And Oxidative Stress

Omega-3 polyunsaturated fatty acids are associated with a lower risk of cardiometabolic diseases. However, docosahexaenoic acid (DHA) is easily oxidized, leading to cellular damage. The present study examined the effects of an increased concentration of DHA in fish oil (80% of total fatty acids) on cardiometabolic risk factors and oxidative stress compared to coconut oil, soybean oil, and fish oil containing eicosapentaenoic acid (EPA) and DHA in a balanced ratio. Forty healthy male Sprague–Dawley rats were supplemented with corresponding oil for 10 weeks. Supplementation with the fish oil containing 80% DHA decreased plasma fat, plasma total cholesterol and muscle fat compared to the coconut oil and the soybean oil. Increasing concentrations of DHA induced incorporation of DHA and EPA in cell membranes and tissues along with a decrease in ω-6 arachidonic acid. The increase in DHA promoted lipid peroxidation, protein carbonylation and antioxidant response. Taken together, the increased concentration of DHA in fish oil reduced fat accumulation compared to the coconut oil and the soybean oil. This benefit was accompanied by high lipid peroxidation and subsequent protein carbonylation in plasma and in liver. In our healthy framework, the slightly higher carbonylation found after receiving fish oil containing 80% DHA might be a protecting mechanism, which fit with the general improvement of antioxidant defense observed in those rats.

scholarly journals Maternal dietary omega-3 fatty acids and placental function

Reproduction ◽  
2014 ◽  
Vol 147 (5) ◽  
pp. R143-R152 ◽  
Author(s):  
Megan L Jones ◽  
Peter J Mark ◽  
Brendan J Waddell
Keyword(s):  
Oxidative Stress ◽  
Fatty Acids ◽  
Cellular Growth ◽  
Gestation Length ◽  
Omega 3 ◽  
Pufa Supplementation ◽  
Placental Function ◽  
Placental Inflammation ◽  
The Impact ◽  
And Oxidative Stress

The developing fetus requires substantial amounts of fatty acids to support rapid cellular growth and activity. Although the fatty acid composition delivered to the fetus is largely determined by maternal circulating levels, the placenta preferentially transfers physiologically important long-chain polyunsaturated fatty acids (LC-PUFAs), particularly omega-3 (n-3) PUFAs. Maternal dietary supplementation with n-3 PUFAs during pregnancy has been shown to increase gestation length, enhance fetal growth, and reduce the risk of pregnancy complications, although the precise mechanisms governing these effects remain uncertain. Omega-3 PUFAs are involved in several physiological pathways which could account for these effects, including anti-inflammatory, pro-resolving, and anti-oxidative pathways. Recent studies have shown that maternal dietary n-3 PUFA supplementation during rat pregnancy can reduce placental oxidative damage and increase placental levels of pro-resolving mediators, effects associated with enhanced fetal and placental growth. Because several placental disorders, such as intrauterine growth restriction, preeclampsia, and gestational diabetes mellitus, are associated with heightened placental inflammation and oxidative stress, there is considerable interest in the potential for dietary n-3 PUFAs as a therapeutic intervention for these disorders. In this study, we review the impact of dietary n-3 PUFAs on placental function, with particular focus on placental inflammation, inflammatory resolution, and oxidative stress.

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