Effects of nitrogen fertilizers on total nitrogen, soluble nitrogen and soluble carbohydrate contents of grass

1962 ◽  
Vol 59 (3) ◽  
pp. 387-392 ◽  
Author(s):  
T. Z. Nowakowski

Italian rye-grass given ammonium sulphate or sodium nitrate at 56 or 112 lb. N/acre was analysed for total nitrogen, soluble nitrogen (non-protein-nitrogen) and soluble carbohydrates.Ten days after applying fertilizer the differences in total-N between the grass receiving 56 and grass receiving 112 lb. N/acre were very small. Total-N in grass decreased with growth, but the effect of the rate of nitrogen on total-N increased. At first the grass given sodium nitrate contained more soluble nitrogen than grass given ammonium sulphate, the difference being greater at 56 lb. N/acre; soluble nitrogen decreased with increasing growth. Ten days after applying fertilizer, the nitrate-N content of grass was very high (ranging from 0·1 to 0·9% in the D.M.) and it gradually decreased. At both levels of nitrogen application, grass given sodium nitrate contained much more nitrate-N than grass given ammonium sulphate. Forty days after applying nitrogen the nitrate-N contents of grass which received 56 and 112 1b. N/acre as ammonium sulphate were 0·039 and 0·222% of the dry matter, respectively; the grass supplied with sodium nitrate gave values of 0·082 and 0·438%.Total soluble carbohydrates in the grass were small early in growth and gradually increased. Nitrogen dressings had little effect on the content of soluble sugars (glucose + fructose + sucrose) but greatly decreased the fructosan. The pattern of changes in the total soluble carbohydrate content followed that in fructosan content. Early in growth, the total soluble carbohydrate/crude protein ratio was very small in grass from all treatments except the ‘control’. This ratio increased with growth and at the last sampling was 2·13 in grass receiving no nitrogen, and in grass supplied with 56 and 112 lb. N/acre as ammonium sulphate it was 1·44 and 0·72 respectively; the corresponding figures for grass receiving sodium nitrate were 1·13 and 0·66. The total soluble carbohydrate carbon/soluble nitrogen ratio in grass with no nitrogen was 18 at the first sampling and it increased gradually, reaching 70 at the last sampling. This ratio was considerably less with all nitrogen treatments than with ‘control’. The values obtained with 112 lb. N/acre were less than those obtained with 561b./acre, irrespective of the form of nitrogen used.The relationship between the soluble carbohydrate carbon content and the soluble nitrogen in grass is illustrated graphically and discussed.

Author(s):  
R.W. Bailey ◽  
R.M. Allison ◽  
K.F. O'Connor

Carbohydrate fractions and total nitrogen were measured in leaves and stems of Wairau lucerne grown in Canterbury. Leaves had lower levels of total soluble carbohydrate (3-5%), cellulose (S-10%) and lignin (a-4(%), and higher levels of total N (3.5-5%) than stems (total soluble carbohydrates l&22%, cellulose 2535%, lignin 4-11% and total N 1.5-4%). During the season, leaves did not change much in composition, whereas stems gradually increased in cellulose and lignin. Wintergrowing lucerne stems contained high total N (up to 4%), low, cellulose (13%) and lignin (4%). Irrigation increased soluble carbohydrate in stems, but not leaves 'in immature luceme. Defoliation of luceme led to a fall in soluble carbohydrate in the standing stems. About 50% of the lucerne total N was easily extracted from the plant as protein. .On a kg/ha basis, a yield of 15, 232 kg of DM (non-irrigated) contained 480 kg of total N, 1,385 kg total soluble carbohydrate and 2,563 kg of cellulose. Over a 70-day summer period, a protein yield of over a metric ton/ha was extracted from irrigated lucerne.


Author(s):  
X. Binopoulos ◽  
G. Kavazis ◽  
A. G. Sficas

AbstractCigarettes with similar parameters were made from various tobaccos of definite types and sources. These were chemically defined according to nicotine, total reducing substances, soluble carbohydrates, polyphenols, total nitrogen, protein nitrogen, total ash, ethanol extractible substances, resins, ''wax'', and raw fiber. The smoke condensate was also determined. The following results were obtained from 17 different kinds of tobacco:1. A positive correlation between the resin content in the leaf and smoke condensate yield. Calculations gave a coefficient of correlation (r) of 0.69 (statistical significant (99 %)).2.The same positive correlation, with a coefficient of correlation of 0.63 (statistical significant (99 %)), was found in relation to the nicotine content of the leaf.3. Other substances determined in the leaf do not seem to be tied to smoke condensate yield.4. The same conclusions are valid when the smoke yield is measured in tars soluble in chloroform


1976 ◽  
Vol 16 (83) ◽  
pp. 875 ◽  
Author(s):  
GJ Osborne ◽  
RR Storrier

The effects were examined in a glasshouse study of three forms of nitrogen fertilizer (urea, ammonium sulphate and sodium nitrate) on the yield, and nitrogen and phosphorus uptake of ryegrass (Lolium perenne) in six representative farming soils from the southern slopes and plains of New South Wales. The results indicated an average recovery of 82 per cent of applied nitrogen across the three forms of fertilizer and the six soils. Ammonium sulphate led to decreases in soil reaction of the order of 0.8-1.5 pH units and lower recoveries; sodium nitrate increased pH. Ammonium sulphate as a source of nitrogen is considered undesirable as it gave lower dry matter yields and nitrogen recovery values compared with the other fertilizer forms. In addition, because of its effect on soil reaction, it could lead to the loss of nutrients by leaching or fixation processes.


1996 ◽  
Vol 2 (5) ◽  
pp. 335-339 ◽  
Author(s):  
F.C. Ibáñez ◽  
A.I. Ordóñez ◽  
M.S. Vicente ◽  
M.I. Torres ◽  
Y. Barcina

Idiazábal cheeses were made employing brining times of 12 h (batch A) and 36 h (batch B). Proteolytic changes in both batches were examined over 270 d of ripening; proteolysis was low in both batches, but lower in batch B than in batch A. Electrophoretic analysis revealed incom plete breakdown of αs and β-caseins at the end of the ripening period, particularly in batch B. The proportion of soluble nitrogen as a percentage of total nitrogen was 17.55% in batch B and 19.48% in batch A, while the proportion of non-protein nitrogen was 11.78% in batch B and 15.16% in batch A. The proportion of non-protein nitrogen as a percentage of soluble nitrogen was 67.17% in batch B and 77.88% in batch A. The free amino acids, the smallest non-protein nitrogen frac tion, attained values of 1203 mg/100 g of dry matter in batch B and 1902 mg/100 g of dry matter in batch A. After 60 d of ripening, the main free amino acids were glutamic acid, valine, leucine, lysine, and phenylalanine in both batches, although levels were higher in the batch with the shorter brining time. There was no clear trend in the non-protein-forming amino acids with either ripening time or brining time.


1955 ◽  
Vol 8 (2) ◽  
pp. 137 ◽  
Author(s):  
HS Mckee ◽  
RN Robertson ◽  
JB Lee

Pea fruits from two crops were sampled at different times from flowering. Changes in the fresh weight, dry weight, starch, soluble carbohydrate, protein nitrogen, and soluble nitrogen in both seeds and hulls were followed in two seasons and related Jo the changes in cell volume in the seeds. In one season respiration rates and phosphate, pectin, and ascorbic acid contents were also investigated. The seeds gained more carbohydrate and nitrogen than was lost by the hulls. Starch and protein were synthesized rapidly by the seeds. The increase in starch content in the seeds was followed by a decrease in soluble carbohydrate content, after which the seed ceased to accumulate water. These metabolic changes are discussed in the light of recent biochemical knowledge, and in relation to more detailed biochemical investigations in progress.


1972 ◽  
Vol 27 (2) ◽  
pp. 285-298 ◽  
Author(s):  
Robert Moss ◽  
J. A. Parkinson

1. In the wild, red grouse live largely on heather, a high-fibre (25%), low-protein (7%) food. Digestibility trials were carried out under semi-natural conditions, with magnesium as a digestibility marker. Two trials were done, one in autumn and one in spring.2. Digestibility of the dry matter varied from 21 to 30% and metabolizable energy from 1.1 to 1.6 kcal/g. These variations were inversely related to intake and could partly be accounted for by facultative variations in holocellulose and lignin digestion.3. Digestion of soluble carbohydrates, protein (measured as α-amino-nitrogen) and holocellulose varied between trials according to the initial concentration in the food. The digestibility of soluble carbohydrate was high (78–83%) in autumn (16% in food) and low (61–66%) in spring (11% in food) and that of protein was relatively low (24–31%) in autumn (6% in food) and high (42–48%) in spring (7% in food). Digestibility of crude fat was 30–33% for four birds and 20% for one bird.4. By comparison with poultry, voluntary intake of dry matter was very high relative to body-weight and intake of energy appeared to be adequate. None the less, all birds lost weight during the trials, presumably for reasons other than energy shortage.5. Urate excretion increased in parallel with body-weight losses, but formed only 2% of the total N output at low weight losses, in which event the main nitrogenous compounds in the droppings were α-amino N (presumably largely from undigested protein), ammonium salts and ornithuric acid.6. The ornithuric acid was presumably a detoxication product of prolignins and possibly tannins and other polyphenols. Its excretion by grouse corresponds to the excretion of hippuric acid by ruminants.


1980 ◽  
Vol 60 (2) ◽  
pp. 365-372 ◽  
Author(s):  
R. E. KARAMANOS ◽  
D. A. RENNIE

Rather marked variations in δa15N values were obtained in a study carried out on samples taken from four soils belonging to the Weyburn soil association. The δa15N of the total N of well-drained depressional profiles dropped sharply with depth and, in contrast, for upper slope positions was relatively constant to a depth of approximately 5 m. This characteristic enrichment in the heavier isotope of total nitrogen of surface horizons may represent long-term immobilization of partially oxidized ammonium N into the organic N fraction; δa15N of the total N more closely represents past soil-forming processes while that of the nitrate N appears to reflect, in addition, recent N cycle stresses.


1991 ◽  
Vol 71 (4) ◽  
pp. 1167-1180 ◽  
Author(s):  
J. W. G. Nicholson ◽  
R. E. McQueen ◽  
E. Charmley ◽  
R. S. Bush

In the first of two experiments to determine the ensiling characteristics of big bale silage, forage was ensiled in round bales (1.2 m diameter × 1.2 m length) at 350–400 (wetter) or 450–500 (drier) g kg−1 dry matter (DM) content by wrapping in plastic. Initial fermentation was more rapid in the wetter bales, as shown by a more rapid and extensive drop in pH and increase in concentration of lactic and acetic acids. The initial lactic acid bacteria (LAB) log counts ranged from 105 and 107 units and were higher for the wetter bales through day 9. By day 58, counts had declined and there were no differences due to moisture level. Water soluble carbohydrate content was higher (P < 0.01) for the drier bales. Ammonia N was higher (P < 0.01) in the wetter bales. Non-protein nitrogen (NPN) did not differ (P > 0.05) due to moisture level at day 9 or day 58. In the second experiment, bales ensiled at (mean ± SE) 390 ± 90 g kg−1 DM content (bale silage) were compared for ensiling characteristics and nutritive value with similar forage chopped and packed in a plastic bag (bag silage). The bag silage had a faster and more extensive drop in pH and increase in concentrations of lactic and acetic acids. Temperatures were approximately 6 °C higher in the bag than in the bale silages over the first 30 d. LAB counts increased faster in the bag silage and then declined and were lower (P < 0.05) than in the bale silage at day 60. Clostridia counts were low in the bag silage and in some bales but exceeded 106 viable spores in other bales. There were no differences between the two silages in digestibility by sheep. However, beef calves fed the bag silage gained more weight with better feed efficiency than those fed bale silage (P < 0.01). Calves fed the bale silage responded to protein (soybean meal) supplementation with increased gain and improved feed conversion, but there was no response with the bag silage. The bag silage had, on average, less NPN (468 vs. 585 g kg−1 total N) than the bale silage. Key words: Silage, big round bales, bag, protein supplementation, cattle performance


HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 584a-584
Author(s):  
Ellen T. Paparozzi ◽  
M. Elizabeth Conley ◽  
Walter W. Stroup

Three cultivars of poinsettia, Freedom Red, Lilo and Red Sails, were grown in a peat:perlite:vermiculite mix according to a commercial production schedule. Twelve selected nitrogen–sulfur fertilizer combinations were applied (125, 150, 175 ppm N with either 12.5, 25, or 37.5 ppm S, 225 and 275 ppm N with either 37.5 or 75 ppm S). The experimental design was a split plot with cultivars as the whole plot and fertilizer levels as the split-plot factor. Mix samples were taken initially, at production week 7 and at the end of the experiment. Nitrate-nitrogen, sulfate-sulfur and total nitrogen were determined. Data were analyzed using SAS PROC MIXED. Visually all cultivars responded similarly to all treatments and were salable. Thus, levels of N as low as 125 or 150 with 12.5 ppm S produced quality plants. Sulfate-S tended to accumulate in the mix while nitrate-N and total N did not. Both nitrate-N and sulfate-S concentrations were affected by an interaction between the cultivar and the amount of S applied with `Freedom' better able to utilize available sulfur. `Lilo' removed more nitrate-N and total N from the mix than `Freedom' which removed more than `Red Sails', but only at specific levels of sulfur. There was no cultivar by nitrogen interaction for any variable measured.


1938 ◽  
Vol 16c (9) ◽  
pp. 377-390 ◽  
Author(s):  
J. Ansel Anderson ◽  
C. Alan Ayre

Determinations of total nitrogen and nitrogen fractions were made on 144 samples of barley representing 12 varieties grown at each of 12 widely separated experimental stations in Canada.A highly significant positive correlation between alcohol-soluble protein nitrogen and total nitrogen was found both within and between varieties. No correlation between total nitrogen and other nitrogen fractions was found between varieties; but significant positive correlations were found within varieties, that for insoluble protein nitrogen being considerably higher than those for total salt-soluble nitrogen, salt-soluble protein nitrogen, and non-protein nitrogen. With increasing total nitrogen, the proportion in salt-soluble form decreases, that in alcohol-soluble form increases, and that in insoluble form remains relatively constant. The results thus offer further support for Bishop's "Protein regularity principle".Mean varietal differences were found with respect to each nitrogen fraction, but elucidation of differences in nitrogen distribution patterns was complicated by the effect of varietal differences in total nitrogen content. Statistical analyses demonstrated the validity of eliminating this effect by adjusting varietal means for fractions to values corresponding to equal total nitrogen contents. When this was done it was found: that the three two-rowed varieties (Charlottetown 80, Hannchen, and Victory) were higher in alcohol-soluble protein nitrogen and lower in insoluble protein nitrogen than any of the six-rowed varieties; and that the four smooth-awned six-rowed varieties (Nobarb, Regal, Velvet, and Wisconsin 38) were lower in total salt-soluble nitrogen and higher in insoluble nitrogen than any of the rough-awned six-rowed varieties (O.A.C. 21, Mensury, Ott. 60, Olli, Peatland, and Pontiac). Owing to the variation between varieties within classes, and the small number of varieties studied, the average differences between the three classes are not statistically significant. Nevertheless, since by comparison with the rough-awned six-rowed varieties, the two-rowed varieties yield higher malt extracts, and the four smooth-awned varieties yield lower malt extracts and are lower in enzymatic activity, the indications of a possible relation between nitrogen distribution and malting quality are interesting.


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