Effects of correcting for microbial contamination and the use of sodium sulphite in neutral detergent fibre analyses on the ruminal fibre degradability of several feeds

2014 ◽  
Vol 153 (2) ◽  
pp. 361-370 ◽  
Author(s):  
J. A. GUEVARA-GONZÁLEZ ◽  
R. MOUHBI ◽  
J. M. ARROYO ◽  
M. R. ALVIR ◽  
J. GONZÁLEZ
Keyword(s):  
Microbial Contamination ◽  
Palm Kernel ◽  
Rapeseed Meal ◽  
Sodium Sulphite ◽  
Neutral Detergent Fibre ◽  
Protein Contamination ◽  
Acid Detergent Fibre ◽  
Beet Pulp ◽  
Gluten Feed

SUMMARYSodium sulphite is used in an optional way to remove insoluble proteins from neutral detergent fibre (NDF) residue. To determine whether the recovery of both NDF and insoluble nitrogen (N) in NDF solution (NDIN) are altered by its use, both parameters were measured in a set of 12 feeds, including cereal grains: maize (MG), rye (RG) and wheat (WG); cereal co-products: maize gluten feed (MGF), distilled dried grains from barley (DDGB) and wheat (DDGW) and wheat bran (WB); protein concentrates: rapeseed meal (RSM) and expeller palm kernel (EPK); dehydrated sugar beet pulp (DBP) and oat (OH) and ryegrass (RGH) hays. Associated effects on thein situeffective degradability (ED) of both NDF and NDIN were also studied in DDGW, WB, RSM, EPK, DBP, OH and RGH. Also, ED of acid detergent fibre (ADF) and its N (ADIN) were studied in hays. Errors due to microbial contamination in the rumen on the ED of NDF, ADF, NDIN and ADIN were also established in these last seven samples using15N infusion methods. Three rumen and duodenum cannulated wethers were used in the study. The sulphite use in NDF solution led to reductions (DDGB, DDGW, RSM and OH) and increases (RG, WG, WB and DBP) of the NDIN proportion, as well as the contribution of crude protein to NDF. These variations were associated with irregular effects on NDF residues and on ED of both NDIN and NDF. As a consequence, sulphite use does not assure the reduction of the insoluble protein contamination and it may even increase it. This methodology may also alter the degradability estimates of NDIN or NDF. Mean ruminal microbial contamination in NDF was 7·0, 10·8, 13·3, 5·4, 12·0, 35·3 and 20·0 g/kg in WB, DDGW, RSM, EPK, DBP, OH and RGH, respectively. The associated contents of microbial N in NDIN were: 59·3, 29·9, 26·2, 19·8, 37·3, 441 and 150 g/kg, respectively. Microbial contamination in ADF and ADIN (g/kg) was 3·6 and 94·5 in OH and 1·7 and 41·2 in RGH. Not correcting this contamination led to consistent undervaluations of ED of NDIN and NDF in all tested feeds, although errors only reached significance for NDIN in hays and DBP. Microbial-corrected ED of NDIN was 0·685, 0·826, 0·481, 0·389, 0·166, 0·718 and 0·425 in WB, DDGW, RSM, EPK, DBP, OH and RGH, respectively, whereas values for ADIN were 0·504 (OH) and 0·469 (RGH).

Effects of the comminution rate and microbial contamination of particles in the rumen on in situ estimates of protein and amino acid digestion of expeller palm kernel and rapeseed meal

2013 ◽  
Vol 94 (7) ◽  
pp. 1291-1298 ◽  
Author(s):  
Javier González ◽  
José M Arroyo ◽  
Rabiaa Mouhbi ◽  
Jesús A Guevara-González ◽  
Vicente J Moya ◽  
...  
Keyword(s):  
Amino Acid ◽  
Microbial Contamination ◽  
Palm Kernel ◽  
Rapeseed Meal ◽  
Acid Digestion

scholarly journals In situstudy of the relevance of bacterial adherence to feed particles for the contamination and accuracy of rumen degradability estimates for feeds of vegetable origin

2006 ◽  
Vol 96 (2) ◽  
pp. 316-325 ◽  
Author(s):  
C. A. Rodríguez ◽  
J. González
Keyword(s):  
Wheat Bran ◽  
Crude Protein ◽  
Microbial Contamination ◽  
Barley Straw ◽  
Cellulose Content ◽  
Asymptotic Values ◽  
Maize Grain ◽  
Gluten Feed ◽  
Single Exponential

Anin situstudy was conducted on four rumen-cannulated wethers to determine (using15N infusion techniques) the microbial contamination (mg bacterial DM or crude protein (CP)/100mg DM or CP) and the associated error on the effective degradability of fourteen feeds: barley and maize grains, soyabean and sunflower meals, full-fat soyabean, maize gluten feed, soyabean hulls, brewers dried grains, sugarbeet pulp, wheat bran, lucerne and vetch-oat hays, and barley and lentil straws. The DM or CP contamination in residues (M) fitted to single exponential or sigmoid curves. A general model (M=m(1−e−ft)j) was proposed to match this fit. Asymptotic values (m) varied from 2·84% to 13·3% and from 2·85% to 80·9% for DM and CP, respectively. Uncorrected results underestimated the effective degradability of both DM (P<0·05) and CP (P<0·01). For CP, this underestimation varied from 0·59% to 13·1%, with a higher but unascertainable error for barley straw. Excluding maize grain, the microbial contamination of both DM and CP, and the associated underestimation of the effective degradability of CP, were positively related to the cellulose content of the feed. The error in the effective degradability of CP was also negatively related to the CP content and its apparent effective degradability (R20·867). This equation allows easier and more accurate estimates of effective degradability, needed to improve protein-rationing systems.

Prediction of crude protein and neutral detergent fibre concentration in residues of in situ ruminal degradation of pasture samples by near-infrared spectroscopy (NIRS)

2016 ◽  
Vol 56 (9) ◽  
pp. 1504 ◽  
Author(s):  
J. P. Keim ◽  
H. Charles ◽  
D. Alomar
Keyword(s):  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Crude Protein ◽  
Near Infrared ◽  
External Validation ◽  
Neutral Detergent Fibre ◽  
Insufficient Amount ◽  
Ruminal Degradation ◽  
Animal Feedstuffs

An important constraint of in situ degradability studies is the need to analyse a high number of samples and often with insufficient amount of residue, especially after the longer incubations of high-quality forages, that impede the study of more than one nutritional component. Near-infrared spectroscopy (NIRS) has been established as a reliable method for predicting composition of many entities, including forages and other animal feedstuffs. The objective of this work was to evaluate the potential of NIRS for predicting the crude protein (CP) and neutral detergent fibre (NDF) concentration in rumen incubation residues of permanent and sown temperate pastures in a vegetative stage. In situ residues (n = 236) from four swards were scanned for their visible-NIR spectra and analysed for CP and NDF. Selected equations developed by partial least-squares multivariate regression presented high coefficients of determination (CP = 0.99, NDF = 0.95) and low standard errors (CP = 4.17 g/kg, NDF = 7.91 g/kg) in cross-validation. These errors compare favourably to the average concentrations of CP and NDF (146.5 and 711.2 g/kg, respectively) and represent a low fraction of their standard deviation (CP = 38.2 g/kg, NDF = 34.4 g/kg). An external validation was not as successful, with R2 of 0.83 and 0.82 and a standard error of prediction of 14.8 and 15.2 g/kg, for CP and NDF, respectively. It is concluded that NIRS has the potential to predict CP and NDF of in situ incubation residues of leafy pastures typical of humid temperate zones, but more robust calibrations should be developed.

Australian and Spanish scientists are collaborating in the domestication of tedera: young Merino sheep grazing a monoculture of tedera in autumn showed preference for certain accessions but no signs of ill health

2013 ◽  
Vol 64 (4) ◽  
pp. 399 ◽  
Author(s):  
C. Oldham ◽  
D. Real ◽  
H. J. Bailey ◽  
D. Thomas ◽  
A. Van Burgel ◽  
...  
Keyword(s):  
Selection Index ◽  
Blood Analysis ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
In Vitro Digestibility ◽  
Sheep Grazing ◽  
Neutral Detergent Fibre ◽  
Relative Preference ◽  
Condition Score ◽  
Acid Detergent Fibre

We hypothesised (i) that sheep grazing a monoculture of tedera (Bituminaria bituminosa (L.) C.H. Stirton var. albomarginata and var. crassiuscula) would not show signs of photosensitisation or ill health, and (ii) that when given free grazing choice they would show a repeatable preference for certain accessions of tedera related to their chemical composition. We tested this by grazing a group of young merino wethers on a monoculture containing seven accessions of tedera for 21 days. General health was assessed via daily visual checks for skin pinkness on the nose and ears, weekly measures of liveweight, condition score, and blood analysis compared with a group of control sheep fed wheaten hay ad libitum. The Chesson–Manly selection index was used to examine the relative preference of sheep for the seven accessions of tedera over the 21 days. Each accession of tedera was sampled weekly to estimate the dry matter on offer, and these samples were also analysed for crude protein, neutral detergent fibre, acid detergent fibre, in vitro digestibility, water soluble carbohydrates, minerals, and concentrations of the furanocoumarins psoralen and angelicin. None of the sheep showed any signs of ill health, with all blood parameters being within the normal reference range. All sheep gained weight and body condition over the 21 days. The difference in the rate of gain in condition score in favour of the sheep grazing tedera over the 21 days (0.014 v. 0.002 unit/sheep.day) was significant (P < 0.001). Sheep showed repeated preference for accessions T31 and T43 (α >0.143). Nutritive value of all accessions of tedera was high. However, only acid detergent fibre and neutral detergent fibre affected the relative preference of the sheep (P < 0.05) and they were only weakly correlated (r2 = 0.208 and 0.165, respectively). We conclude that there are accessions of tedera that are preferred by sheep that could be used to fill the autumn feed gap experienced in the south of Western Australia without any risk to the health of the sheep.

Impact of four fiber-rich supplements on nutrient digestibility, colostrum production, and farrowing performance in sows

2021 ◽  
Vol 99 (9) ◽  
Author(s):  
Takele Feyera ◽  
Liang Hu ◽  
Maria Eskildsen ◽  
Thomas S Bruun ◽  
Peter K Theil
Keyword(s):  
Dry Matter ◽  
Palm Kernel ◽  
Nutrient Digestibility ◽  
Extra Energy ◽  
Lower Blood ◽  
Nonstarch Polysaccharides ◽  
Gross Energy ◽  
Soy Hulls ◽  
Beet Pulp ◽  
The Impact

Abstract This study aimed to investigate the impact of dietary fiber (DF) sources on sow and litter performance, and apparent total tract digestibility (ATTD) of gross energy (GE) and nutrients. A total of 48 sows were stratified for body weight at mating and randomly assigned to one of four DF sources (mixed fiber [MF], palm kernel expellers [PKE], sugar beet pulp [SBP], or soy hulls [SH]) and fed the diet from mating until farrowing. Within DF treatments, sows were supplemented with one of two extra energy sources (glycerol or sugar dissolved in water), whereas a third group (control) received water from day 108 of gestation until farrowing. The number of total born, live-born, and stillborn pigs; birth time and birth weight of the pigs; farrowing duration; and farrowing assistance (FA) were recorded. Live-born pigs were weighed again at 12 and 24 h after birth to record weight gain, which was used to estimate intake and yield of colostrum. Blood samples were collected once daily from day −3 relative to farrowing until day 1 after farrowing in sows and once from selected pigs right after birth. Fecal samples were collected on day 114 of gestation and colostrum at 0, 12, 24, and 36 h after onset of farrowing. Intake of soluble and insoluble nonstarch polysaccharides (NSP) was greater for SBP (P &lt; 0.001) and PKE (P &lt; 0.001) supplemented sows, respectively, when compared with other groups. Farrowing duration and stillbirth rate were not affected by DF sources, but PKE and SH supplemented sows had greater FA than SBP and MF supplemented sows (P &lt; 0.001). Extra energy supplement did not improve the farrowing performance. Concentration (P = 0.02) and output (P = 0.04) of dry matter in colostrum, and ATTD of GE (P &lt; 0.001) and crude protein (CP; P &lt; 0.001) were lower for PKE supplemented sows than in sows from the remaining groups. Intake of insoluble NSP correlated negatively with ATTD of GE (P &lt; 0.001) and CP (P &lt; 0.001). Concentrations of glucose (P &lt; 0.001), lactate (P &lt; 0.001), CO2 (P &lt; 0.001), and HCO3 (P &lt; 0.001) in sows blood were increased with time progress relative to farrowing. Newborn pigs from PKE supplemented sows had greater concentration of lactate (P = 0.02) and lower blood pH (P = 0.02) than the remaining treatments. In conclusion, PKE supplement reduced ATTD of GE and CP, and concentration and output of dry matter in colostrum but increased FA. Results of this experiment indicated that the use of PKE as a fiber source for late gestating sows should be avoided.

scholarly journals Effects of the comminution rate and microbial contamination of particles in the rumen on accuracy ofin situestimates of digestion of protein and amino acids of dehydrated sugar beet pulp

2013 ◽  
Vol 152 (1) ◽  
pp. 166-174 ◽  
Author(s):  
J. GONZÁLEZ ◽  
J. M. ARROYO ◽  
J. A. GUEVARA-GONZÁLEZ ◽  
R. MOUBI ◽  
O. PIQUER ◽  
...  
Keyword(s):  
Amino Acids ◽  
Sugar Beet ◽  
Crude Protein ◽  
Microbial Contamination ◽  
Negative Impact ◽  
Degradation Kinetics ◽  
Sugar Beet Pulp ◽  
Fresh Weight Basis ◽  
Beet Pulp ◽  
Composite Samples

SUMMARYEffects of the correction of microbial contamination (using15N techniques) and of considering the comminution rate (kc) of particles in the rumen on effective estimates of the ruminally undegraded (RU) fraction and its intestinal effective digestibility (IED) were examined in a sample of dehydrated sugar beet pulp (DBP) generating composite samples (from rumen-incubated residues) representative of the chemical composition of RU. Tested fractions were dry matter (DM), organic matter (OM, tested only for RU), crude protein (CP) and amino acids (AA). The study was performed on three rumen and duodenum cannulated wethers fed with a 2 : 1 (fresh weight basis) chopped oat hay-to-concentrate diet supplied at 40 g DM/kg BW0·75in six equal meals per day. The DBP showed sigmoid degradation kinetics: the fractional degradation rate increased by 5·8 times as time (h) increased from 0 to∞. Thekcrate (measured in the diet concentrate) represented 5·74% of the total rumen retention time of particles. As a result, the RU of DM was over-evaluated by 6·53% whenkcwas not considered. Microbial contamination of RU was high as in DM as in CP. Therefore, the overestimation of RU of DM was increased to 12·2% when this contamination was not corrected. The lack of this correction also led to large over-evaluations of RU and IED of CP and AA. As a result, the overestimation of the intestinal digested fraction was 40·9% for CP and 45·0% for total analysed AA. This overestimation varied largely among AA (from 18·9 to 88·7%). Corrected proportions of RU and IED were also variable among AA. Hypotheses on the causes of this variability are given. Resultant changes in the AA profile of the intestinal digested protein had some negative impact on the supply of essential AA and cysteine without affecting lysine. This problem is limited because the microbial protein synthesized from DBP fermentation in the rumen is largely predominant in the AA supply to the host.

Rumen degradation of straw 9. Effect of cellulase and ammonia treatment on different varieties of rice straws and their botanical fractions

1990 ◽  
Vol 50 (2) ◽  
pp. 309-317 ◽  
Author(s):  
Y. Nakashima ◽  
E. R. Ørskov
Keyword(s):  
Propionic Acid ◽  
Rice Straw ◽  
Leaf Blade ◽  
Soluble Fraction ◽  
Leaf Sheath ◽  
Ammonia Treatment ◽  
Neutral Detergent Fibre ◽  
Rumen Degradation ◽  
Significant Difference ◽  
Acid Detergent Fibre

ABSTRACTThe composition and degradability of four varieties of rice straw were examined. The varieties were tall or short and early or late maturing. The straws and their botanical parts (leaf blade plus leaf sheath, internode, node and chaff) varied in acid-detergent fibre, ash and silica contents, but not in nitrogen and neutral-detergent fibre. There were some trends but no significant difference in proportions of parts between varieties. Milled samples of whole straw and its parts were either untreated, treated with a cellulase preparation plus propionic acid, treated with ammonia, or treated with cellulase then ammonia. Rumen degradability was measured by incubating the samples in polyester bags suspended in the rumen of sheep. The soluble fraction and potential degradability were greater in untreated short and early than in tall and late varieties, but rates of degradation did not vary significantly. Internode was more degradable than leaf. Cellulase and/or ammonia treatments increased the soluble fraction, especially in tall and late varieties, and increased potential degradabiiity except in internode. Degradability correlated positively with ash and silica contents.

Odd-chain fatty acids as markers of the microbial colonisation of freshly-ingested grass and microbial contamination of dacron bag residues

2002 ◽  
Vol 2002 ◽  
pp. 50-50 ◽  
Author(s):  
E. J. Kim ◽  
R. T. Evans ◽  
J. K. S. Tweed ◽  
D. R. Davies ◽  
R. J. Merry ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Microbial Contamination ◽  
Technical Problems ◽  
Rumen Microbes ◽  
Rumen Degradation ◽  
Plant Enzymes ◽  
Odd Chain Fatty Acids ◽  
Chain Fatty Acids

The overall objective of our work is to assess the relative contributions of plant enzymes and rumen microbes to rumen degradation of freshly-ingested herbage. In situ techniques have been used extensively to compare rumen degradation characteristics of feeds, though there remain technical problems associated with microbial contamination of residues after incubation. We hypothesised that techniques to study microbial contamination might also provide insights into microbial colonisation. Our earlier studies (Lee et al., 1999) identified distinctive odd-chain fatty acids that could be used as microbial markers. A dacron bag study was conducted to examine the influence of dacron bag rinsing techniques on DM disappearance and microbial contamination in residues from fresh grass, assessed using odd-chain fatty acids as markers.

Evaluation of mathematical models to describe protein fraction degradation kinetics of various oilseed meals

2009 ◽  
Vol 2009 ◽  
pp. 171-171
Author(s):  
M Danesh Mesgaran ◽  
T Tashakkori ◽  
A Heravi Moussavi ◽  
S Danesh Mesgaran ◽  
A Vakili ◽  
...  
Keyword(s):  
Mathematical Models ◽  
Goodness Of Fit ◽  
Degradation Kinetics ◽  
Direct Method ◽  
Rapeseed Meal ◽  
Insoluble Protein ◽  
Degradation Kinetic ◽  
Negative Exponential ◽  
Kinetics Of

In situ procedure is a direct method of measuring the rumen degradation kinetic of a feed nutrient. Data obtained by this technique are generally analysed using an exponential curve (Ørskov and McDonald, 1979). However, very low attention has been paid to choice of mathematical model to fit the curves and goodness-of-fit of the model. Lopez et al. (1999) pointed out that the disappearance of some feed components, particularly structural carbohydrates, exhibits a larger variety of forms than for crude protein (CP). In the present study, two different mathematical models of a straight line or a negative exponential (France et al., 1990; and Ørskov and McDonald, 1979) were selected to evaluate in situ degradation kinetics of protein fractions including true protein (TP), neutral-detergent insoluble protein (NDIP) and acid-detergent insoluble protein (ADIP) of various oilseed meals (cottonseed meal (CSM), soyabean meal (SM) and rapeseed meal (RM)).

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