Fatty liver in dairy cows post partum is associated with decreased concentration of plasma triacylglycerols and decreased activity of lipoprotein lipase in adipocytes

2005 ◽  
Vol 72 (2) ◽  
pp. 129-137 ◽  
Author(s):  
A Marc Van den Top ◽  
Arie Van Tol ◽  
Hans Jansen ◽  
Math JH Geelen ◽  
Anton C Beynen
Keyword(s):  
Adipose Tissue ◽  
Fatty Liver ◽  
Dairy Cows ◽  
Lipoprotein Lipase ◽  
Post Partum ◽  
Free Access ◽  
Lipid Transfer ◽  
Dry Period ◽  
Plasma Lcat ◽  
Ad Libitum

Cholesterol and phospholipid concentrations in serum lipoproteins, plasma activities of lecithin:cholesterol acyltransferase (LCAT) and phospholipid transfer protein (PLTP) and lipoprotein lipase (LPL) activity in adipose tissue biopsies were measured ante and post partum in dairy cows given either free or restricted access to feed during the dry period. After parturition, all cows were fed ad libitum. The purpose of this study was to try to understand the earlier observed marked drop post partum in plasma triacylglycerol (TAG) in terms of lipoprotein metabolism in cows developing fatty liver post partum. As would be expected, free access to feed during the dry period induced a rise of hepatic TAG concentrations post partum associated with a decrease in plasma TAG levels. Total and free cholesterol concentrations in the VLDL, IDL, LDL and HDL2 fractions fell immediately after parturition. VLDL and IDL cholesterol concentrations remained at a constant, low level during the entire sampling period post partum, whereas the drop in LDL and HDL2 cholesterol post partum was followed by a rebound rise. Plasma LCAT and PLTP activities decreased by on average 19% and 33%, respectively, after parturition and then rose to values seen before parturition, but there was no effect of feeding regimen during the dry period. Activities of LCAT and PLTP were significantly correlated with cholesterol and phospholipid concentrations in LDL and HDL2. Plasma LCAT activity, as measured with exogenous substrate, and PLTP activity were both positively correlated with HDL3 phospholipid levels. LPL activity in adipose tissue dropped after parturition, the drop being smaller after feeding ad libitum during the dry period. It is concluded that the drop in adipose tissue LPL activity post partum is at variance with the simultaneous fall in plasma TAG. Possibly, the decrease in adipose tissue LPL activity helps to channel fatty acids away from adipose tissue into the udder. The post-partum changes in lipid transfer proteins in the blood are in line with the changes observed in the levels of the lipoproteins.

scholarly journals Effect of increasing digestible undegraded protein supply to dairy cows in late gestation on the yield and composition of milk during the subsequent lactation

1996 ◽  
Vol 63 (2) ◽  
pp. 201-213 ◽  
Author(s):  
J. M. Moorby ◽  
R. J. Dewhurst ◽  
S. Marsden
Keyword(s):  
Dairy Cows ◽  
Milk Protein ◽  
Barley Straw ◽  
Protein Supplement ◽  
Dry Period ◽  
Body Protein ◽  
Grass Silage ◽  
Management Regime ◽  
Protein Pool ◽  
Ad Libitum

AbstractEffects of feeding a protein supplement to dairy cows during the dry period on performance during the following lactation were investigated in two experiments. Holstein-Friesian cows were paired towards the end of lactation, and, after drying off, one of each pair received a typical dry cow management regime of ad libitum grass silage (experiment 1), or a mix of grass silage and distillers' grains or pressed beet pulp (experiment 2). The other cows were offered restricted access to the same basal diet, together with ad libitum access to barley straw and 0·5 kg/day high protein maize gluten meal. During the following lactation, animals from both groups were treated without reference to dry period treatment, and were offered equal access to the same lactation diet. Data were analysed by analysis of variance of experiment means and by parallel curve analysis using sample means. In experiment 1, milk yields were similar (27·2 v. 27·9 (s.e.d. 2·12) kg/day for control and supplemented animals respectively) but milk protein yields, and hence concentrations, were significantly higher (P < 0·001) from supplemented animals (28·9 v. 31·8 (s.e.d. 0·58) g/kg). In experiment 2, milk yields were significantly higher (P < 0·001) from supplemented animals (mean 33·3 v. 35·4 (s.e.d. 1·66) kg/day; however, milk protein yields were also significantly increased (P < 0·001) and the change in milk protein concentration was small. No difference in dry-matter intake was recorded in a subset of animals during early lactation in experiment 2. It is hypothesized that the maternal labile body protein pool was maintained or replenished during the dry period by the provision of the protein supplement, and that this had a significant effect on subsequent lactation performance.

scholarly journals Microelement Supplementation in Dairy Cows by Mineral Lick

2009 ◽  
Vol 78 (1) ◽  
pp. 29-36
Author(s):  
Štěpán Krys ◽  
Eva Lokajová ◽  
Aleš Podhorský ◽  
Leoš Pavlata
Keyword(s):  
Dairy Cows ◽  
Selenium Concentration ◽  
Control Group ◽  
Dry Period ◽  
Total Consumption ◽  
Zn Concentration ◽  
Ad Libitum ◽  
Mn Concentration ◽  
Experimental Group ◽  
Mineral Lick

The aim of this experiment was to verify the option of providing microelement supplementation to dairy cows in the dry period through supplemented mineral lick, and then to compare the content of microelements in the blood, colostrum and milk. The experiment was carried out on a farm with Czech Fleckvieh dairy cows. We formed an experimental group (E), supplied with mineral lick ad libitum enhanced with organic forms of microelements (Se, Zn, Cu, Mn) for four to five weeks before parturition and 1 week after parturition. The control group (C) did not receive any mineral lick. The use of mineral lick by cows was very uneven, its total consumption oscillated between 0 to 250 g of lick per animal per day. Lick feeding did not result in evidential increase of concentrations of monitored microelements in the cows’ blood, colostrum and milk. Only a tendency to increased concentrations of Se and Cu in the blood of group E of cows was observed; the average Se concentration increased by 60 μg l-1 (Cu by 9 μmol l-1), whereas in group C, Se increased only by 30 μg l-1 (Cu by 6 μg l-1). The concentration of microelements in colostrum after parturition dropped quickly. Significant drops were determined for Se, Cu and Zn on the first (for Mn on the second) day after parturition. Selenium concentration in the first colostrum was 5 times higher than in milk, Cu concentration 3 times higher, Zn concentration 4 times higher and Mn concentration 1.3 times higher.

Body composition and performance of autumn-calving Holstein-Friesian dairy cows during lactation: metabolic activityin vitroof subcutaneous adipose tissue

1992 ◽  
Vol 1992 ◽  
pp. 104-104
Author(s):  
A. Walsh ◽  
J. D. Sutton ◽  
D. E. Beever
Keyword(s):  
Adipose Tissue ◽  
Dairy Cows ◽  
Body Fat ◽  
Post Partum ◽  
Additional Source ◽  
Genetic Potential ◽  
Holstein Friesian ◽  
And Performance ◽  
Lactation Cycle ◽  
Subcutaneous Adipose

In early lactation dairy cows use stored body fat to provide an additional source of energy to support milk production and these reserves are replaced when energy requirements for milk synthesis are reduced. The extent to which body fat is mobilized depends on the nutritional state of the animal and its genetic potential. These changes are accompanied by substantial alterations in the intermediary metabolism of adipose tissue. The aim of this work was to investigate the timing and magnitude of changes in synthetic and lipolytic pathways in adipose tissue from dairy cows during a lactation cycle and at three levels of energy intake.Fifty four cows were fed grass silagead libitumand a concentrate ration at 3(L), 6(M) or 9(H) kg DM/day (Sutton et al 1992). An initial group of six cows was slaughtered at 2 to 4 dayspost partum(week 0) followed by two cows from each treatment at weeks 2, 5, 8, 11, 14, 19, 24 and 29.

Ultrasonographic measurement of liver, portal vein, hepatic vein and perivisceral adipose tissue in high-yielding dairy cows with fatty liver during the transition period

2018 ◽  
Vol 85 (4) ◽  
pp. 431-438 ◽  
Author(s):  
Enrico Fiore ◽  
Laura Perillo ◽  
Massimo Morgante ◽  
Elisabetta Giudice ◽  
Barbara Contiero ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Fatty Liver ◽  
Dairy Cows ◽  
Transition Period ◽  
Body Condition Score ◽  
Liver Lipid ◽  
Perivascular Adipose Tissue ◽  
Liver Lipid Content ◽  
Mesenteric Fat ◽  
Condition Score

The aim of the present study was to evaluate the potential for diagnosis of fatty liver by means of ultrasonographic measurement of liver and perivisceral adipose tissue as an alternative to blood indicators of lipomobilization and liver biopsy in periparturient high-yielding dairy cows. Thirty cows were enrolled and divided into two groups. The evaluation of body condition score (BCS), non-esterified fatty acids (NEFA), β-hydroxybutyrate (BHB), liver and perivisceral adipose tissue ultrasonographic measurement and histological liver lipid content (GdL) was performed at 15 ± 5 d prepartum (T0), 10 ± 2 d postpartum (T1), 30 ± 2 d postpartum (T2) and 50 ± 2 d postpartum (T3). Mesenteric fat thickness (the thickness of the perivascular adipose tissue) measured on ultrasound was shown to be an independent determinant of fatty liver. The cut-off of the ultrasonographic evaluation of the liver may be useful as a first and practical approach in identifying fatty liver. In conclusion, a non-invasive and reliable diagnostic method for predicting the risk of fatty liver in high yielding dairy cows has been demonstrated.

Influence of Different Levels of Dietary Energy on Performance of Double Suckled Cows and Calves

1986 ◽  
Vol 1986 ◽  
pp. 106-106
Author(s):  
M.A.S. Khan ◽  
J.H. Topps ◽  
P.J. Broadbent
Keyword(s):  
Dairy Cows ◽  
Good Condition ◽  
Beef Cows ◽  
Free Access ◽  
Dietary Energy ◽  
Early Lactation ◽  
Concentrate Mixture ◽  
Soyabean Meal ◽  
Ad Libitum ◽  
Different Levels

The winter feeding of beef cows represents a high, proportion of the costs of calf production. Attempts to minimise cost include the use of beef (♂) x dairy cows (♀) which have the ability to suckle two calves and to mobilise body reserves during periods of underfeeding in early lactation. Khan and Topps (1985) observed that cows in good condition at calving could maintain milk production and raise two good calves when given 110 MJ ME/day in a diet which included a source of undegradable protein. Subsequently a preliminary experiment was carried out with three single suckled and nine double suckled cows to examine the effect of energy level on the performance of cows and calves.Three single suckled cows and nine double suckled cows i.e. six with a natural plus foster calf and three with twin calves produced by embryo transfer were used in this experiment. The levels of energy that were examined were 70 or 90, 80 or 100 and 90 or 110 MJ ME/day for single and double-suckled cows respectively, supplied in diets containing ammonia treated straw, barley and fishmeal. Nine of the cows were placed into trios according to calving date and age, with a trio consisting of a cow with a natural calf, a cow with a natural and foster calf, and a cow with twin calves. Each trio was allocated at random to one of the three levels of energy. Similarly, the remaining three cows with a natural and foster calf were allocated at random to 90, 100 or 110 MJ ME/day. The calves were given good quality hay ad libitum and free access to a concentrate mixture up to a maximum intake of 2 kg/head/day. The concentrate mixture consisted of (g/kg) 800 barley, 150 soyabean meal and 50 molassine meal. The experiment started on the 22nd day of lactation and was of 20 weeks duration for most of the cows.

scholarly journals Prepartum dietary energy intake alters adipose tissue transcriptome profiles during the periparturient period in Holstein dairy cows

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Andrea Minuti ◽  
Massimo Bionaz ◽  
Vincenzo Lopreiato ◽  
Nicole A. Janovick ◽  
Sandra L. Rodriguez-Zas ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Immune System ◽  
Lipid Metabolism ◽  
Dairy Cows ◽  
Antigen Processing ◽  
Fatty Acid Biosynthesis ◽  
Mtor Signaling ◽  
Energy Requirements ◽  
Dry Period ◽  
Periparturient Period

Abstract Background The aim of the study was to investigate the effect of energy overfeeding during the dry period on adipose tissue transcriptome profiles during the periparturient period in dairy cows. Methods Fourteen primiparous Holstein cows from a larger cohort receiving a higher-energy diet (1.62 Mcal of net energy for lactation/kg of dry matter; 15% crude protein) for ad libitum intake to supply 150% (OVR) or 100% (CTR) of energy requirements from dry off until parturition were used. After calving, all cows received the same lactation diet. Subcutaneous adipose tissue (SAT) biopsies were collected at − 14, 1, and 14 d from parturition (d) and used for transcriptome profiling using a bovine oligonucleotide microarray. Data mining of differentially expressed genes (DEG) between treatments and due to sampling time was performed using the Dynamic Impact Approach (DIA) and Ingenuity Pathway Analysis (IPA). Results There was a strong effect of over-feeding energy on DEG with 2434 (False discovery rate-corrected P < 0.05) between OVR and CTR at − 14 d, and only 340 and 538 at 1 and 14 d. The most-impacted and activated pathways in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database that were highlighted by DIA analysis at − 14 d in OVR vs. CTR included 9 associated with carbohydrate metabolism, with ‘Pyruvate metabolism’, ‘Glycolysis/gluconeogenesis’, and ‘Pentose phosphate pathway’ among the most-activated. Not surprisingly, OVR led to marked activation of lipid metabolism (e.g. ‘Fatty acid biosynthesis’ and ‘Glycerolipid metabolism’). Unexpected metabolic pathways that were activated at − 14 d in OVR included several related to metabolism of amino acids (e.g. branched chain) and of cofactors and vitamins (thiamin). Among endocrine and immune system pathways, at − 14 d OVR led to marked activation of ‘PPAR signalling’ and ‘Antigen processing and presentation’. Among key pathways affected over time in OVR, a number were related to translation (e.g. mTOR signaling), endocrine/immune signaling (CXCR4 and IGF1), and lipid metabolism (oxidative phosphorylation) with greater activation in OVR vs. CTR specifically at − 14 d. Although statistical differences for several pathways in OVR vs. CTR nearly disappeared at 1 and 14 vs. − 14 d, despite the well-known catabolic state of adipose depots after calving, the bioinformatics analyses suggested important roles for a number of signaling mechanisms at − 14 vs. 14 than 1 vs. -14 d. This was particularly evident in cows fed to meet predicted energy requirements during the dry period (CTR). Conclusions Data underscored a strong activation by overfeeding energy of anabolic processes in the SAT exclusively prepartum. The study confirmed that higher-energy diets prepartum drive a transcriptional cascade of events orchestrated in part by the activation of PPARγ that regulate preadipocyte differentiation and lipid storage in SAT. Novel aspects of SAT biology to energy overfeeding or change in physiologic state also were uncovered, including the role of amino acid metabolism, mTOR signaling, and the immune system.

scholarly journals Continuous milking of dairy cows disrupts timing of peak IgG concentration appearance in mammary secretions

2014 ◽  
Vol 81 (4) ◽  
pp. 403-409 ◽  
Author(s):  
Craig R Baumrucker ◽  
Rahel S Zbinden ◽  
H Anette van Dorland ◽  
Gerrit J Remmelink ◽  
Bas Kemp ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Dairy Cows ◽  
Post Partum ◽  
Experimental Period ◽  
Subsequent Pregnancy ◽  
Main Concern ◽  
Dairy Production ◽  
Dry Period ◽  
Timing Effect

The length of the dry period in commercial dairy production is under close scrutiny. While the main concern is the composition and volume of milk produced, the evaluation of colostrum quality under these new paradigms has suggested a decline in IgG concentrations, while some reports indicate no change. Colostrum quality has been defined as an adequate concentration (>50 mg/ml) of immunoglobulin in the secretions to provide the newborn with maximal disease resistance. We investigated the appearance of IgG in mammary pre- and post partum secretions in cows without a dry period (continuously milked, Dry0) and compared the secretions with cows that experienced a dry period of 60 d (Dry60). Blood was collected during the experimental period and plasma analysed for progesterone (P4) and prolactin (Prl). Approximately −6 d relative to parturition, the Dry0 animals exhibited increased concentration of IgG in their secretions to an average of ∼35 mg/ml that remained rather constant through subsequent pregnancy and following parturition. Dry0 cows were producing an average IgG concentration in parturition colostrum of 44·2±17·6 mg/ml that was not different than that of controls (66·86±16·8 mg/ml). However, Dry0 cows exhibited high variation, different peak times (day) of IgG concentration including times that occurred both pre and post parturition. IgG mass of the Dry0 cows remained rather constant pre- and post partum and did not show the same declining mass following parturition that was shown for the Dry60 cows. The change in plasma P4 and Prl were shown to have no timing effect on colostrum IgG concentration.

Effects of dry period diet on feed intake, body composition and subsequent lactational performance of dairy cows

1996 ◽  
Vol 1996 ◽  
pp. 4-4
Author(s):  
R.J. Dewhurst ◽  
D.W.R. Davies ◽  
W.J. Fisher ◽  
K. Aston
Keyword(s):  
Body Composition ◽  
Dairy Cows ◽  
Feed Intake ◽  
Milk Protein ◽  
Access Time ◽  
Dry Period ◽  
Grass Silage ◽  
Lactational Performance ◽  
Forage Intake ◽  
Ad Libitum

Previous studies (Moorbyet al, 1994) have shown that supplementing grass silage with undegradable protein (UDP) during the dry period can lead to increased yields of milk protein and lactose. These studies have often involved restriction of forage intake through limitation of access time and the feeding of straw. This experiment was conducted to assess the effect of UDP supplementation withad libitumaccess to grass silage as well as the effects of restricting forage intakes by including straw in the diets of dry cows.

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