The effect of yeast and distillery by-products on the fermentation in the rumen simulation technique (rusitec)

1992 ◽  
Vol 1992 ◽  
pp. 210-210
Author(s):  
C.J. Newbold ◽  
R.J. Wallace
Keyword(s):  
Rumen Fermentation ◽  
Simulation Technique ◽  
Yeast Cells ◽  
Feed Additive ◽  
Yeast Culture ◽  
Microbial Protein ◽  
Ruminant Diets ◽  
Commercial Yeast ◽  
Low Levels ◽  
By Products

The practice of adding low levels of non-commensal yeast and fungi to ruminant diets is increasingly gaining acceptance as a means of manipulating rumen fermentation to benefit production. Reported benefits include an increased degradability of forages in the rumen and an improved flow of microbial protein from the rumen (Williams and Newbold, 1990).Distillery by-products, such as pot ale syrup, are commonly included in ruminant diets as an energy source. However, pot ale syrup contains a substantial number of yeast cells. The aim of the present study was to establish if different yeasts and yeast-containing by-products had similar effects on rumen fermentation to those found with a commercial yeast culture feed additive.Two commercial Saccharomyces cerevisiae preparations (Alkosel, Alko Biotechnology, Finland and Yea-sacc, Alltech, UK), active dried baker's yeast (United Distillers, UK) and two pot ale syrups from the Inchgower and Dailuaine distilleries were compared for their effects on the fermentation in the rumen simulation technique (Rusitec).

The effect of yeast culture on rumen fermentation: Growth of the yeast in the rumen and the requirement for viable yeast cells

1993 ◽  
Vol 1993 ◽  
pp. 175-175 ◽  
Author(s):  
S.M. El Hassan ◽  
C.J. Newbold ◽  
R.J. Wallace
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rumen Fermentation ◽  
Bacterial Activity ◽  
Yeast Cells ◽  
Yeast Culture ◽  
Current Experiment ◽  
Microbial Protein ◽  
Live Yeast

Yeast culture (YC) based on Saccharomyces cerevisiae has been reported to stimulate bacterial activity within the rumen, leading to increases in ruminal fibre digestion and microbial protein flow from the rumen (Wallace and Newbold, 1992). Dawson (1987) suggested that S. cerevisiae might grow in the rumen. Newbold et al (1990) found no evidence for the growth of S. cerevisiae in the rumen of sheep when the numbers of live yeast in the rumen were measured at various times after a diet contain YC had been consumed. The current experiment was designed to investigate further the possibility that S. cerevisiae grows in the rumen and to establish the importance of viable yeast cells in the action of YC in the rumen.

Effects of yeast culture on rumen fermentation and liveweight gain in bulls fed isonitrogenous diets of barley/urea or barley/soya

1992 ◽  
Vol 1992 ◽  
pp. 211-211
Author(s):  
S.M. Elhassan ◽  
R.J. Wallace ◽  
C.J. Newbold ◽  
X.B. Chen ◽  
I.E. Edwards ◽  
...  
Keyword(s):  
Microbial Growth ◽  
Rumen Fluid ◽  
Rumen Fermentation ◽  
Feed Additive ◽  
Yeast Culture ◽  
Purine Derivatives ◽  
Stomach Tube ◽  
Cane Molasses ◽  
Microbial Nitrogen ◽  
Soyabean Meal

Yeast culture (YC) based on Saccharomyces cerevisiae is gaining increasing acceptance as a feed additive for ruminants. Production responses to YC have been reported in growing and lactating animals. It has been suggested that these responses are at least partly due to enhanced microbial growth in and microbial nitrogen flow from the rumen (Williams and Newbold, 1990). The aim of the present study was to investigate the effect of YC on rumen fermentation, microbial growth and liveweight gain in bulls fed isonitrogenous diets containing either urea or soya.Thirty two Limousin cross Friesian bulls (liveweight 344 kg) were allocated to treatments on the basis of previous growth rate and initial liveweight. Animals received mixed diets of barley/urea (92% rolled barley, 5% cane molasses, 1.5% urea and 1.5% minerals/vitamin mix) or barley/soya (86.75% rolled barley, 5% cane molasses, 0.75% urea, 6% soyabean meal and 1.5% minerals/vitamin mix) plus or minus 1.5 kg/ tonne YC (Yea-sacc1026 , Alltech, UK) (Table 1). YC was added to the diet prior to mixing. All rations were offered ad libitum twice daily until slaughter (460 kg). Rumen fluid samples were withdrawn by stomach tube 5 and 10 weeks after the beginning of the trial. The outflow of microbial nitrogen from the rumen was estimated from the appearance of purine derivatives in the urine as described previously (Chen et al., 1990).

Influence of ionophores on in vitro fermentation by rumen fluid from sheep receiving yeast culture (Yeasacc; YC)

1991 ◽  
Vol 1991 ◽  
pp. 78-78
Author(s):  
C.J. Newbold ◽  
R.J. Wallace ◽  
I.M. Nevison
Keyword(s):  
Feed Efficiency ◽  
Rumen Fluid ◽  
Basal Diet ◽  
Rumen Fermentation ◽  
Animal Production ◽  
Yeast Culture ◽  
Microbial Protein ◽  
In Vitro Fermentation ◽  
Wide Range

A wide range of compounds has been described which have the potential to improve animal production by manipulating the rumen fermentation. Prominent among these rumen modifiers are the ionophores. Ionophores, such as monensin and tetronasin, improve feed efficiency, partly by increasing the flow of amino-N from the rumen and partly by stimulating the production of propionate in the rumen with an associated reduction in the production of methane (Russell and Strobel, 1988). Recently there has been increasing interest in the use of yeast culture (YC) and other fungal preparation to modify the rumen fermentation. These products have been shown to increase bacterial numbers within the rumen with an associated increase in the breakdown of fibre and supply of microbial protein (Williams and Newbold, 1990). YC has also been reported to increase the production of propionate in the rumen. Little appears to be known about the effect a combination YC and an ionophore would have on the rumen fermentation. This study describes the effects of the ionophores monensin and tetronasin on the fermentation of hay by rumen fluid from sheep fed a basal diet with or without YC.

Effect of supplementation of sucrose and lactose with sodium bicarbonate on rumen metabolism and microbial protein synthesis in sheep

1999 ◽  
Vol 1999 ◽  
pp. 28-28 ◽  
Author(s):  
A. Hussain ◽  
E. L. Miller
Keyword(s):  
Protein Synthesis ◽  
Sodium Bicarbonate ◽  
Rumen Fermentation ◽  
Bacterial Protein ◽  
Microbial Protein ◽  
Rumen Ph ◽  
Rumen Metabolism ◽  
Ruminant Diets ◽  
Energy Supplements ◽  
Main Factor

Sucrose and lactose are used as energy supplements in ruminant diets. In our previous study (Hussain and Miller, 1998) lactose maintained a higher rumen pH, increased organic matter and neutral detergent fibre digestion in the rumen, reduced the number of rumen protozoa and increased microbial (bacterial) protein flow to the duodenum compared with sucrose. However, it was not clear whether the effect of lactose on pH or on reduction of protozoa was the main factor increasing rumen fermentation and microbial protein flow. The objective of the present study was to examine the effect of isoenergetic amounts of sucrose and lactose as supplements (equivalent to 50 g glucose) on rumen fermentation and metabolism in the presence of sodium bicarbonate to maintain rumen pH.

The response of limousin x friesian steers fed silage and concentrates to the addition of supplemental yeast culture (yea-sacc) and/or antibiotic additive (avotan)

1991 ◽  
Vol 1991 ◽  
pp. 95-95
Author(s):  
I E Edwards ◽  
J H Topps ◽  
M El Sheikh ◽  
G F M Paterson
Keyword(s):  
Growth Medium ◽  
Rumen Fermentation ◽  
Feed Additive ◽  
Yeast Culture ◽  
Animal Performance

Supplementation with Yea-sacc - yeast (Saccharomyces cerivisiae ‘1026’) and its growth medium or with Avotan - an antibiotic feed additive has been found by several workers to alter rumen fermentation patterns and animal performance. This experiment investigated the effect of inclusion of these supplements on their own or in combination in the concentrates of silage fed steers on rumen fermentation and animal performance.

Combined effects of aspergillus oryzae fermentation extract (amaferm; ao) and monensin on fermentation in the rumen simulation technique (rusitec)

1991 ◽  
Vol 1991 ◽  
pp. 112-112 ◽  
Author(s):  
N. McKain ◽  
C.J. Newbold ◽  
R.J. Wallace
Keyword(s):  
Aspergillus Oryzae ◽  
Bacterial Growth ◽  
Methane Production ◽  
Rumen Fermentation ◽  
Simulation Technique ◽  
Feed Additive ◽  
Combined Effects ◽  
Growth Promoters

Monensin, an ionophore, and AO, a fungal feed additive, are growth promoters which modify rumen fermentation. Previous studies have shown that monensin increased propionate, the efficiency of bacterial growth and the flow of protein from the rumen and decreased methane production and fibre digestion (Schelling, 1984), whereas AO increased bacterial numbers and fibre digestion and lowered methane production (Frumholtzet al., 1989; Fondevilaet al., 1990). The aim of the present study was to determine, using Rusitec, the effects on rumen fermentation of combining these additives.

Rumen Probiosis: effects of addition of yeast culture (viable yeast (Saccharomyces cerevisiae) plus growth medium) on patterns of rumen fermentation

1989 ◽  
Vol 1989 ◽  
pp. 160-160 ◽  
Author(s):  
P. E. V. Williams ◽  
G. M. Innes
Keyword(s):  
Growth Medium ◽  
Yeast Cells ◽  
Yeast Culture ◽  
Productive Capacity ◽  
Yeast Saccharomyces Cerevisiae ◽  
Beneficial Effects ◽  
Rumen Metabolism ◽  
Micro Organisms ◽  
High Forage ◽  
By Products

The use of brewery by-products which contain varying quantities of live and dead yeast cells as nitrogen supplements in diets for ruminants is well documented.There have been several recent reports of micro-organisms, previously considered as being totally aerobic, multiplying and exhibiting growth in the rumen and in rumen simulators and conferring beneficial effects on cellulolysis and growth or productive capacity of the animal. Dawson (1987) reported that certain strains of yeast (Saccharomyces cerivisae) multiplied when introduced into the rumen. Although at some point extensive lysis occurs with extrusion of the cell contents (Bruning and Yokoyama, 1988). The present experiment was carried out to assess the effects of including yeast culture (YS) (Saccharomyces cerevisae, plus growth medium: 5x106 organisms/g) in diets for ruminants. The responses in terms of effects on rumen metabolism were monitored in three cannulated steers.The aim of the experiment was to determine the effects on the rumen metabolism and the degradability of a forage using the nylon bag technique (Ørskov and McDonald, 1970) when yeast culture was added to two contrasting diets and given to young steers. The diets were either a high forage diet of hay or a mixture of forage plus concentrate given in such a manner as to induce a negative associative effect.

scholarly journals Dose–Response Effects of 3-Nitrooxypropanol Combined with Low- and High-Concentrate Feed Proportions in the Dairy Cow Ration on Fermentation Parameters in a Rumen Simulation Technique

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1784
Author(s):  
Matthias Schilde ◽  
Dirk von Soosten ◽  
Liane Hüther ◽  
Susanne Kersten ◽  
Ulrich Meyer ◽  
...  
Keyword(s):  
Dose Response ◽  
Gas Production ◽  
Hydrogen Gas ◽  
Simulation Technique ◽  
Mitigation Strategies ◽  
Feed Additive ◽  
Future Research ◽  
Dependent Manner ◽  
Dose Dependent

Methane (CH4) from ruminal feed degradation is a major pollutant from ruminant livestock, which calls for mitigation strategies. The purpose of the present 4 × 2 factorial arrangement was to investigate the dose–response relationships between four doses of the CH4 inhibitor 3-nitrooxypropanol (3-NOP) and potential synergistic effects with low (LC) or high (HC) concentrate feed proportions (CFP) on CH4 reduction as both mitigation approaches differ in their mode of action (direct 3-NOP vs. indirect CFP effects). Diet substrates and 3-NOP were incubated in a rumen simulation technique to measure the concentration and production of volatile fatty acids (VFA), fermentation gases as well as substrate disappearance. Negative side effects on fermentation regarding total VFA and gas production as well as nutrient degradability were observed for neither CFP nor 3-NOP. CH4 production decreased from 10% up to 97% in a dose-dependent manner with increasing 3-NOP inclusion rate (dose: p < 0.001) but irrespective of CFP (CFP × dose: p = 0.094). Hydrogen gas accumulated correspondingly with increased 3-NOP dose (dose: p < 0.001). In vitro pH (p = 0.019) and redox potential (p = 0.066) varied by CFP, whereas the latter fluctuated with 3-NOP dose (p = 0.01). Acetate and iso-butyrate (mol %) decreased with 3-NOP dose, whereas iso-valerate increased (dose: p < 0.001). Propionate and valerate varied inconsistently due to 3-NOP supplementation. The feed additive 3-NOP was proven to be a dose-dependent yet effective CH4 inhibitor under conditions in vitro. The observed lack of additivity of increased CFP on the CH4 inhibition potential of 3-NOP needs to be verified in future research testing further diet types both in vitro and in vivo.

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