The Role of Collagen, Fibronectin, Chondronectin and Laminin in Cell Adhesion

1982 ◽  
Vol 40 ◽  
pp. 102-105
Author(s):  
Hynda K. Kleinman ◽  
George R. Martin
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Blood Cells ◽  
Basement Membranes ◽  
Extracellular Matrices ◽  
Structural Support ◽  
Tissue Organization ◽  
Attachment Proteins ◽  
Surrounding Extracellular Matrix

Most cells other than blood cells interact with extracellular matrices composed of various types of collagens, proteoglycans and a newly described group of glycoproteins termed the attachment proteins. Variations in the composition of the extracellular matrix determine the uniqueness of tissues, such as cartilage, bone, dermis and basement membranes. These matrices have numerous functions, including providing structural support to rigid tissues (cartilage, tooth and bone), regulating the passage of macromolecules (kidney basement membrane), and allowing tissues to stretch (blood vessels and skin). In addition, these matrices have potent influences upon the cells which populate them. The anchorage, growth, differentiation, and motility of the resident cells are all determined by their surrounding extracellular matrix. Such matrices are critically important in embryonic development, tissue organization and wound healing.

scholarly journals Roles of Proteoglycans and Glycosaminoglycans in Wound Healing and Fibrosis

2015 ◽  
Vol 2015 ◽  
pp. 1-20 ◽  
Author(s):  
Shibnath Ghatak ◽  
Edward V. Maytin ◽  
Judith A. Mack ◽  
Vincent C. Hascall ◽  
Ilia Atanelishvili ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Cells ◽  
White Blood Cells ◽  
Cell Types ◽  
Repair Process ◽  
Epithelial Layer ◽  
Extracellular Molecules ◽  
Living Tissues ◽  
Type Of Injury

A wound is a type of injury that damages living tissues. In this review, we will be referring mainly to healing responses in the organs including skin and the lungs.Fibrosisis a process of dysregulated extracellular matrix (ECM) production that leads to a dense and functionally abnormal connective tissue compartment (dermis). In tissues such as the skin, the repair of the dermis after wounding requires not only thefibroblaststhat produce the ECM molecules, but also the overlying epithelial layer (keratinocytes), theendothelial cells, andsmooth muscle cellsof the blood vessel and white blood cells such asneutrophilsandmacrophages, which together orchestrate the cytokine-mediated signaling and paracrine interactions that are required to regulate the proper extent and timing of the repair process. This review will focus on the importance of extracellular molecules in the microenvironment, primarily the proteoglycans and glycosaminoglycan hyaluronan, and their roles in wound healing. First, we will briefly summarize the physiological, cellular, and biochemical elements of wound healing, including the importance of cytokine cross-talk between cell types. Second, we will discuss the role of proteoglycans and hyaluronan in regulating these processes. Finally, approaches that utilize these concepts as potential therapies for fibrosis are discussed.

scholarly journals Lung development, repair and cancer: A study on the role of MMP20 gene in adenocarcinoma

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250552
Author(s):  
Pooi Ling Mok ◽  
Arun Neela Kumar Anandasayanam ◽  
Hernandez Maradiaga Oscar David ◽  
Jiabei Tong ◽  
Aisha Farhana ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wound Healing ◽  
Lung Adenocarcinoma ◽  
Lung Development ◽  
A549 Cells ◽  
Control Group ◽  
Wound Healing Assay ◽  
Transfected Cells ◽  
Tissue Organization

Multiple matrix metalloproteinases have significant roles in tissue organization during lung development, and repair. Imbalance of proteinases may lead to chronic inflammation, changes in tissue structure, and are also highly associated to cancer development. The role of MMP20 is not well studied in lung organogenesis, however, it was previously shown to be present at high level in lung adenocarcinoma. The current study aimed to identify the functional properties of MMP20 on cell proliferation and motility in a lung adenocarcinoma in vitro cell model, and relate the interaction of MMP20 with other molecular signalling pathways in the lung cells after gaining tumoral properties. In this study, two different single guide RNA (sgRNAs) that specifically targeted on MMP20 sites were transfected into human lung adenocarcinoma A549 cells by using CRISPR-Cas method. Following that, the changes of PI3-K, survivin, and MAP-K mRNA gene expression were determined by Real-Time Polymerase Chain Reaction (RT-PCR). The occurrence of cell death was also examined by Acridine Orange/Propidium Iodide double staining. Meanwhile, the motility of the transfected cells was evaluated by wound healing assay. All the data were compared with non-transfected cells as a control group. Our results demonstrated that the transfection of the individual sgRNAs significantly disrupted the proliferation of the A549 cell line through suppression in the gene expression of PI3-K, survivin, and MAP-K. When compared to non-transfected cells, both experimental cell groups showed reduction in the migration rate, as reflected by the wider gaps in the wound healing assay. The current study provided preliminary evidence that MMP20 could have regulatory role on stemness and proliferative genes in the lung tissues and affect the cell motility. It also supports the notion that targeting MMP20 could be a potential treatment mode for halting cancer progression.

scholarly journals LncRNA XIST promotes extracellular matrix synthesis, proliferation and migration by targeting miR-29b-3p/COL1A1 in human skin fibroblasts after thermal injury

2019 ◽  
Vol 52 (1) ◽  
Author(s):  
Wei Cao ◽  
Youping Feng
Keyword(s):  
Extracellular Matrix ◽  
Cell Proliferation ◽  
Wound Healing ◽  
Western Blot ◽  
Thermal Injury ◽  
Human Skin Fibroblasts ◽  
Cell Proliferation And Migration ◽  
And Migration ◽  
Proliferation And Migration

Abstract Background Long noncoding RNAs (lncRNAs) have been reported to be associated with dermis process during burn wound healing. This study aimed to investigate the role of lncRNA X-inactive specific transcript (XIST) in human skin fibroblasts (HSF) and extracellular matrix (ECM) as well as the regulatory network of XIST/microRNA-29b-3p (miR-29b-3p)/collagen 1 alpha 1 (COL1A1). Methods The wound samples were collected from 25 patients with deep partial thickness burn at day 5 after burn. The thermal injured model was established using HSF cells. The expressions of XIST, miR-29b-3p and COL1A1 were measured by quantitative real-time polymerase chain reaction and western blot. ECM synthesis, cell proliferation and migration were detected by western blot, cell counting kit-8 and trans-well assays, respectively. The interaction between miR-29b-3p and XIST or COL1A1 was explored by bioinformatics analysis and luciferase reporter assay. Results The expressions of XIST and COL1A1 were enhanced but miR-29b-3p expression was decreased after thermal injury. XIST overexpression promoted ECM synthesis, cell proliferation and migration in thermal injured HSF cells. However, XIST knockdown played an opposite effect. miR-29b-3p overexpression inhibited ECM synthesis, cell proliferation and migration, which was reversed by XIST. COL1A1 silence suppressed ECM synthesis, cell proliferation and migration by miR-29b-3p targeting. Moreover, COL1A1 up-regulation weakened the effect of XIST silence on ECM synthesis and HSF cell function. Conclusion XIST promoted ECM synthesis, cell proliferation and migration by sponging miR-29b-3p and targeting COL1A1 in HSF cells after thermal injury, indicating the promoting role of XIST in wound healing.

The Role of Inflammation and Blood Cells in Wound Healing

2013 ◽  
pp. 73-89 ◽  
Author(s):  
Linda H. Chao ◽  
Martha M. Murray
Keyword(s):  
Wound Healing ◽  
Blood Cells

scholarly journals Leucocyte-Rich Platelet-Rich Plasma Enhances Fibroblast and Extracellular Matrix Activity: Implications in Wound Healing

2020 ◽  
Vol 21 (18) ◽  
pp. 6519
Author(s):  
Jeannie Devereaux ◽  
Narges Dargahi ◽  
Sarah Fraser ◽  
Kulmira Nurgali ◽  
Dimitrios Kiatos ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Proliferation ◽  
Wound Healing ◽  
Blood Cells ◽  
Platelet Rich Plasma ◽  
Autologous Blood ◽  
Human Fibroblasts ◽  
White Blood Cells ◽  
High Concentration ◽  
Gene Expressions

Background: Platelet-rich plasma (PRP) is an autologous blood product that contains a high concentration of platelets and leucocytes, which are fundamental fibroblast proliferation agents. Literature has emerged that offers contradictory findings about leucocytes within PRP. Herein, we elucidated the effects of highly concentrated leucocytes and platelets on human fibroblasts. Methods: Leucocyte-rich, PRP (LR-PRP) and leucocyte-poor, platelet-poor plasma (LP-PPP) were compared to identify their effects on human fibroblasts, including cell proliferation, wound healing and extracellular matrix and adhesion molecule gene expressions. Results: The LR-PRP exhibited 1422.00 ± 317.21 × 103 platelets/µL and 16.36 ± 2.08 × 103 white blood cells/µL whilst the LP-PPP demonstrated lower concentrations of 55.33 ± 10.13 × 103 platelets/µL and 0.8 ± 0.02 × 103 white blood cells/µL. LR-PRP enhanced fibroblast cell proliferation and cell migration, and demonstrated either upregulation or down-regulation gene expression profile of the extracellular matrix and adhesion molecules. Conclusion: LR-PRP has a continuous stimulatory anabolic and ergogenic effect on human fibroblast cells.

scholarly journals The Versatile Role of Matrix Metalloproteinase for the Diverse Results of Fibrosis Treatment

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4188 ◽  
Author(s):  
Hong-Meng Chuang ◽  
Yu-Shuan Chen ◽  
Horng-Jyh Harn
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Cardiovascular Diseases ◽  
De Novo ◽  
Biological Processes ◽  
Tissue Formation ◽  
Ecm Degradation ◽  
Excessive Secretion ◽  
Wound Tissue

Fibrosis is a type of chronic organ failure, resulting in the excessive secretion of extracellular matrix (ECM). ECM protects wound tissue from infection and additional injury, and is gradually degraded during wound healing. For some unknown reasons, myofibroblasts (the cells that secrete ECM) do not undergo apoptosis; this is associated with the continuous secretion of ECM and reduced ECM degradation even during de novo tissue formation. Thus, matrix metalloproteinases (MMPs) are considered to be a potential target of fibrosis treatment because they are the main groups of ECM-degrading enzymes. However, MMPs participate not only in ECM degradation but also in the development of various biological processes that show the potential to treat diseases such as stroke, cardiovascular diseases, and arthritis. Therefore, treatment involving the targeting of MMPs might impede typical functions. Here, we evaluated the links between these MMP functions and possible detrimental effects of fibrosis treatment, and also considered possible approaches for further applications.

The Role of Extracellular Matrix in Corneal Wound Healing

Cornea ◽  
2013 ◽  
Vol 32 ◽  
pp. S43-S45 ◽  
Author(s):  
Osamu Yamanaka ◽  
Takayoshi Sumioka ◽  
Shizuya Saika
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Corneal Wound Healing ◽  
Corneal Wound
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