Effects of osmotic priming on dormancy and storability of tomato (Lycopersicon esculentumMill.) seeds

AbstractFreshly harvested tomato (Lycopersicon esculentumMill, cv. Moneymaker) seeds were osmotically primed for 8 d in −1.0 MPa PEG-6000 solution and dried to about 6% water content for storage. Such so–called ‘fresh PEG priming’ enhanced seed germination and improved seedling performance as compared with the untreated control. Fresh PEG priming neither alleviated seed dormancy nor promoted DNA replication as was the case when seeds were dried upon harvest and subsequently primed in PEG (normal PEG priming). However, the addition of 10 μM GA4+7to the osmotic priming solution triggered replicative DNA synthesis of fresh-priming seeds and further enhanced the germination process. After 5 months of storage in ambient temperature conditions, fresh PEG-primed seeds maintained more positive effects gained from priming, whereas, normal PEG-primed seeds had lost the promoting effects on germination. Normal PEG-primed seeds were much more susceptible to controlled deterioration than fresh PEG-primed seeds. It is suggested that the advancement of germination is negatively correlated with seed storability. The mechanisms of seed priming in relation to nuclear replication activities and physical changes are discussed.

Download Full-text

The efficacy of different seed priming agents for promoting sorghum germination under salt stress

PLoS ONE ◽

10.1371/journal.pone.0245505 ◽

2021 ◽

Vol 16 (1) ◽

pp. e0245505

Author(s):

Xiaofei Chen ◽

Ruidong Zhang ◽

Yifan Xing ◽

Bing Jiang ◽

Bang Li ◽

...

Keyword(s):

Salt Stress ◽

Seed Germination ◽

Germination Rate ◽

Principal Component ◽

Dry Weight ◽

Seed Priming ◽

Nacl Stress ◽

Future Research ◽

Fresh Weight ◽

Positive Effects

Sorghum [Sorghum bicolor (L.) Moench] seed germination is sensitive to salinity, and seed priming is an effective method for alleviating the negative effects of salt stress on seed germination. However, few studies have compared the effects of different priming agents on sorghum germination under salt stress. In this study, we quantified the effects of priming with distilled water (HP), sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2), and polyethylene glycol (PEG) on sorghum seed germination under 150 mM NaCl stress. The germination potential, germination rate, germination index, vigor index, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight were significantly reduced by salt stress. Different priming treatments alleviated the germination inhibition caused by salt stress to varying degrees, and 50 mM CaCl2 was the most effective treatment. In addition, the mitigation effect of priming was stronger on root traits than on shoot traits. Mitigation efficacy was closely related to both the type of agent and the concentration of the solution. Principal component analysis showed that all concentrations of CaCl2 had higher scores and were clearly distinguished from other treatments based on their positive effects on all germination traits. The effects of the other agents varied with concentration. The priming treatments were divided into three categories based on their priming efficacy, and the 50, 100, and 150 mM CaCl2 treatments were placed in the first category. The 150 mM KCl, 10% PEG, HP, 150 mM NaCl, 30% PEG, and 50 mM KCl treatments were placed in the second category, and the 100 mM NaCl, 100 mM KCl, 20% PEG, and 50 mM NaCl treatments were least effective and were placed in the third category. Choosing appropriate priming agents and methods for future research and applications can ensure that crop seeds germinate healthily under saline conditions.

Download Full-text

Cryopreservation of primed tobacco seeds

Journal of Seed Science ◽

10.1590/2317-1545v40n4207216 ◽

2018 ◽

Vol 40 (4) ◽

pp. 415-421

Author(s):

Camila Aparecida Lopes ◽

Maria Laene Moreira de Carvalho ◽

Ana Cristina de Souza ◽

João Almir Oliveira ◽

Dayliane Bernardes de Andrade

Keyword(s):

Seed Germination ◽

Ambient Temperature ◽

Silica Gel ◽

Saline Solution ◽

Seed Priming ◽

Potassium Nitrate ◽

Water Bath ◽

Tobacco Seeds ◽

Physiological Quality

Abstract: Priming is a commercial technique used to increase the speed and uniformity of seed germination. However, the physiological quality of primed seeds is usually negatively affected during storage. Tobacco seeds of the cultivar BAT 2101 were used to investigate whether primed seeds could be cryopreserved. The most suitable substances, type of drying, and reheating during priming and cryopreservation of tobacco seeds were studied. Seed priming was performed with water, spermidine, and potassium nitrate, and drying was carried out with silica gel and a saturated saline solution. Seeds were cryopreserved in liquid nitrogen for 24 h and reheated in a water bath for two and five minutes and at ambient temperature. Tobacco seeds primed with spermidine and water can be cryopreserved without loss of physiological quality when quickly dried on silica gel and reheated in a water bath for two minutes.

Download Full-text

Effect of Priming Techniques on Seed Germination Characteristics of C. Maxima Duch

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.474-476.36 ◽

2011 ◽

Vol 474-476 ◽

pp. 36-39 ◽

Cited By ~ 1

Author(s):

Yong Dong Sun ◽

Xin Zheng Li ◽

He Lian Yang ◽

Li Sun

Keyword(s):

Polyethylene Glycol ◽

Seed Germination ◽

Seed Priming ◽

Distilled Water ◽

Nacl Solution ◽

Peg 6000 ◽

Germination Process ◽

Germination Characteristics ◽

Successful Technique

The present study was conducted to investigate the effect of seed priming techniques on germination characteristics of C. maxima Duch. cultivar (Beiguan). Treatments were combinations of 3 levels of priming (distilled water, NaCl and PEG6000) and non-priming (control) with 3 replications. Concentrations of NaCl solution were 50, 100, 150, 200 mmol•L-1, and polyethylene glycol (PEG) 6000 were 10%, 20%, 30%, 40%, respectively. Seeds were primed using the above priming materials for 24 hours at 20°C in the dark, respectively. The results showed that different priming techniques could have various effects on germination of Beiguan seeds. Hydropriming (distilled water), NaCl priming and PEG6000 priming (10%) all improved the germination characteristics of Beiguan, compared to the control. NaCl priming was more effective than hydropriming and PEG6000 priming and was the most successful technique in this study. These findings indicated that seed priming techniques could accelerate germination process and were simple and cheap, we should propose these methods to farmers.

Download Full-text

The Effect of Osmopriming on Seed Germination and Early Seedling Characteristics of Carum carvi L.

Agriculture ◽

10.3390/agriculture10040094 ◽

2020 ◽

Vol 10 (4) ◽

pp. 94

Author(s):

Iman Mirmazloum ◽

Attila Kiss ◽

Éva Erdélyi ◽

Márta Ladányi ◽

Éva Zámboriné Németh ◽

...

Keyword(s):

Polyethylene Glycol ◽

Seed Germination ◽

Germination Rate ◽

Petri Dish ◽

Seed Priming ◽

Germination Percentage ◽

Positive Effects ◽

Carum Carvi ◽

Early Seedling ◽

Seedling Characteristics

Two experiments (in Petri dishes and in soil) were conducted to investigate the effects of osmopriming on seed germination and the early seedling characteristics of caraway (Carum carvi L. var. annua). The priming treatments in the Petri dish experiment were: polyethylene glycol (5%, 10% and 20%), KNO3 (0.5%, 1% and 2%) and KCL (1%, 2% and 4%) with three different soaking times (12, 24 and 36 h) along with control (non-primed seeds). Only polyethylene glycol and H2O were applied in the cell tray experiment, which were then compared with the non-primed seeds. In the Petri dish experiment, all three priming reagents significantly enhanced seedling length, with the most effective treatments being 5% PEG, 2% KNO3 and 1% KCL for 12 h. The plumule dry weights were also increased significantly after PEG (20% for 36 h), KNO3 (2% for 24 and 36 h) and KCL (1% for 12 h) treatments in comparison with the control. In the soil experiment, osmopriming with PEG significantly improved the germination rate (GR) and percentage, the plumule dry and fresh weights and the plumule length of caraway seedlings when compared with the control. A 23% higher germination percentage was recorded for the seeds treated with 5% PEG for 24 h as compared with the non-primed seeds. The PEG-primed seeds produced significantly longer seedlings when treated with 5% PEG for 24 h. All of the applied PEG treatments significantly enhanced the plumule fresh and dry weights, with the best outcomes being after 5% PEG (24 h) and 10% PEG (36 h) treatments, respectively. The 12-h hydro-priming also significantly enhanced all of the studied germination parameters when compared to the control. The results of the presented experiments show the significant positive effects of seed priming on caraway germination and how early seedling performance can easily be adopted by producers.

Download Full-text

Effect of Treating Acid Sulfate Soils with Phosphate Solubilizing Bacteria on Germination and Growth of Tomato (Lycopersicon esculentum L.)

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18178919 ◽

2021 ◽

Vol 18 (17) ◽

pp. 8919

Author(s):

Jae Hwan Kim ◽

So-Jeong Kim ◽

In-Hyun Nam

Keyword(s):

Seed Germination ◽

Lycopersicon Esculentum ◽

Untreated Control ◽

Phosphate Solubilizing Bacteria ◽

Control Soil ◽

Acid Sulfate Soils ◽

Bacterial Strains ◽

Acid Sulfate ◽

Phosphate Solubilizing ◽

Sulfate Soils

Acid sulfate soils contain sulfide minerals that have adverse environmental effects because they can lead to acidic drainage and prevent the establishment of vegetation. The current study examined the effect of a novel method for the restoration of these soils and the promotion of germination and plant growth. Thus, we isolated two strains of phosphate solubilizing bacteria, Methylobacterium sp. PS and Caballeronia sp. EK, characterized their properties, and examined their effects in promoting the growth of tomato plants (Lycopersicon esculentum L.) in acid sulfate soil. Compared with untreated control soil, treatment of acid sulfate soils with these bacterial strains led to increased seed germination, growth of plants with more leaves, and plants with greater levels of total-adenosine tri-phosphate (tATP). Relative to the untreated control soil, the addition of Caballeronia sp. EK led to a 60% increase in seed germination after 52 days, growth of plants with more than 3 times as many leaves, and a 45.2% increase in tATP after 50 days. This strain has potential for use as a plant biofertilizer that promotes vegetation growth in acid sulfate soils by improving the absorption of phosphorous.

Download Full-text

Gibberellic Acid Biosynthesis During Dehydration Phase of Priming Increases Seed Vigour of Tomato

10.21203/rs.3.rs-522160/v1 ◽

2021 ◽

Author(s):

Seethalakshmi S ◽

Umarani R ◽

Djanaguiraman Maduraimuthu

Keyword(s):

Seed Germination ◽

Gibberellic Acid ◽

Seed Priming ◽

Seedling Vigour ◽

Seed Vigour ◽

Seedling Traits ◽

Physiological Processes ◽

Germination Process ◽

Priming Process ◽

Subsequent Effect

Abstract Priming of seed is intended to reduce the time to germination through activation of pre-germinative processes. Seed priming is controlled hydration and followed by a drying (dehydration) process. The physiological processes during hydration (imbibition) were studied in detail in tomato. In contrast, gibberellic acid (GA) changes during the dehydration phase were not studied in detail. We hypothesize that there would be a change in the GA concentration during the dehydration phase of the seed priming process, which may influence the vigour characteristics of the resultant seedling. The objective was to understand the influence dehydration phase of seed priming on GA biosynthesis and its subsequent effect on seed germination and seedling traits of tomato. First, the hydroprimed and unprimed seeds were re-imbibed for 3, 6, 9, and 12 h to initiate the germination process, and the GA concentration and seedling vigour associated parameters were recorded. In the second experiment, the imbibed seeds were dehydrated for 3, 6, 9, and 12 h, then re-imbibed for 3, 6, 9, and 12 h to understand the effect of dehydration on the GA concentration and its associated traits. Results revealed that hydroprimed seeds had a higher GA concentration and seedling vigour than unprimed seeds. The seeds that are completely dehydrated for 12 h had the highest GA and seed vigour parameters. Therefore, increased vigour of hydroprimed seeds is due to the higher levels of GA accumulated during the dehydration phase of seed priming, which can improve seed germination and seedling vigour of tomato.

Download Full-text

Seed priming for increased seed germination and enhanced seedling vigor of winter rice

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/756/1/012047 ◽

2021 ◽

Vol 756 (1) ◽

pp. 012047

Author(s):

M P Anwar ◽

R Jahan ◽

M R Rahman ◽

A K M M Islam ◽

F M J Uddin

Keyword(s):

Seed Germination ◽

Seed Priming ◽

Seedling Vigor

Download Full-text

QTL analysis of seed germination traits in tobacco (Nicotiana tabacum L.)

Journal of Applied Genetics ◽

10.1007/s13353-021-00623-6 ◽

2021 ◽

Author(s):

Monika Agacka-Mołdoch ◽

Mian Abdur Rehman Arif ◽

Ulrike Lohwasser ◽

Teresa Doroszewska ◽

Ramsey S. Lewis ◽

...

Keyword(s):

Nicotiana Tabacum ◽

Seed Germination ◽

Doubled Haploid ◽

Mapping Population ◽

Area Under The Curve ◽

Linkage Groups ◽

Polygenic Control ◽

Nicotiana Tabacum L ◽

Controlled Deterioration ◽

Germination Traits

AbstractGenetic mapping of seed germination traits has been performed with many plant species. In tobacco, however, investigations are rare. In the present study, a bi-parental mapping population consisting of 118 doubled haploid lines and derived from a cross between ‘Beinhart-1000’ and ‘Hicks’ was investigated. Four germination-related traits, total germination (TG), normal germination (NG), time to reach 50% of total germination (T50), and the area under the curve after 200 h of germination (AUC) were considered by examining seeds either untreated or after a moderate controlled deterioration (CD). Quantitative trait loci were found for all traits distributed on 11 out of the 24 linkage groups. It was demonstrated that, as in many other species, germination-related traits are very complex and under polygenic control.

Download Full-text

Accelerating Seed Germination and Juvenile Growth of Sorghum (Sorghum bicolor L. Moench) to Manage Climate Variability through Hydro-Priming

Atmosphere ◽

10.3390/atmos12040419 ◽

2021 ◽

Vol 12 (4) ◽

pp. 419

Author(s):

Siaka Dembélé ◽

Robert B. Zougmoré ◽

Adama Coulibaly ◽

John P. A. Lamers ◽

Jonathan P. Tetteh

Keyword(s):

Seed Germination ◽

Climate Variability ◽

Germination Rate ◽

Dry Weight ◽

Seed Priming ◽

Seedling Development ◽

Hot Water ◽

Crop Failure ◽

Simple Method ◽

Early Season

Agriculture in Mali, a country in Sahelian West Africa, strongly depends on rainfall and concurrently has a low adaptive capacity, making it consequently one of the most vulnerable regions to climate change worldwide. Since early-season drought limits crop germination, and hence growth, ultimately yield during rain-fed depending on production is commonly experienced nowadays in Mali. Germination and establishment of key crops such as the staple sorghum could be improved by seed priming. The effects of hydro-priming with different water sources (e.g., distilled, tap, rain, river, well water) were evaluated respectively for three priming time durations in tepid e.g., at 25 °C (4, 8, and 12 h) and by hot water at 70 °C (in contrast to 10, 20, and 30 min.) in 2014 and 2015. Seed germination and seedling development of nine sorghum genotypes were monitored. Compared to non-primed seed treatments, hydro-priming significantly [p = 0.01] improved final germination percentage, germination rate index, total seedling length, root length, root vigor index, shoot length, and seedling dry weight. The priming with water from wells and rivers resulted in significant higher seed germination (85%) and seedling development, compared to the three other sources of water. Seed germination rate, uniformity, and speed were enhanced by hydro-priming also. It is argued that hydro-priming is a safe and simple method that effectively improve seed germination and seedling development of sorghum. If used in crop fields, the above most promising genotypes may contribute to managing early season drought and avoid failure of seed germination and crop failure in high climate variability contexts.

Download Full-text