Effect of Priming Techniques on Seed Germination Characteristics of C. Maxima Duch

The present study was conducted to investigate the effect of seed priming techniques on germination characteristics of C. maxima Duch. cultivar (Beiguan). Treatments were combinations of 3 levels of priming (distilled water, NaCl and PEG6000) and non-priming (control) with 3 replications. Concentrations of NaCl solution were 50, 100, 150, 200 mmol•L-1, and polyethylene glycol (PEG) 6000 were 10%, 20%, 30%, 40%, respectively. Seeds were primed using the above priming materials for 24 hours at 20°C in the dark, respectively. The results showed that different priming techniques could have various effects on germination of Beiguan seeds. Hydropriming (distilled water), NaCl priming and PEG6000 priming (10%) all improved the germination characteristics of Beiguan, compared to the control. NaCl priming was more effective than hydropriming and PEG6000 priming and was the most successful technique in this study. These findings indicated that seed priming techniques could accelerate germination process and were simple and cheap, we should propose these methods to farmers.

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Effects of Osmopriming on Germination and Vigour Traits of Bersim Clover (Trifolium alexandrinum L.)

Notulae Scientia Biologicae ◽

10.15835/nsb244735 ◽

2010 ◽

Vol 2 (4) ◽

pp. 59-63 ◽

Cited By ~ 3

Author(s):

Hossein Reza ROUHI ◽

Reza Tavakkol AFSHARI ◽

Seyed Amir MOOSAVI ◽

Mohammad Hossain GHARINEH

Keyword(s):

Polyethylene Glycol ◽

Osmotic Potential ◽

Seed Priming ◽

Control Group ◽

Trifolium Alexandrinum ◽

Distilled Water ◽

Peg 6000 ◽

Crop Establishment ◽

Germination Characteristics ◽

Polyethylene Glycol 6000

Previous studies suggested that fast and uniform germination is important for good crop establishment. The present study was conducted to investigate the possibility of increasing germination characteristics by seed priming techniques. An experiment was conducted with three replicates and two treatments including: 2 different priming duration (8 and 12 hours) and 6 osmotic potential of PEG 6000 solutions (-0.8, -1,-1.2,-1.4,-1.6 Mpa) and distilled water as a control group). The priming solutions were prepared using polyethylene glycol 6000 (PEG). Our results showed that the most effective osmotic potential in improving germination characteristics of Trifolium alexandrium is -0.8 MPa for 16 hours.

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Stevia rebaudiana (Bert) Bertoni: influence of osmotic stress and seed priming on seed germination under laboratory conditions

Acta Scientiarum Agronomy ◽

10.4025/actasciagron.v39i3.32951 ◽

2017 ◽

Vol 39 (3) ◽

pp. 379 ◽

Cited By ~ 2

Author(s):

Keila Regina Hossa ◽

José Walter Pedroza Carneiro ◽

Terezinha Aparecida Guedes ◽

Alessandro Lucca Braccini

Keyword(s):

Polyethylene Glycol ◽

Seed Germination ◽

Osmotic Stress ◽

Stevia Rebaudiana ◽

Seed Priming ◽

Initial Time ◽

Peg 6000 ◽

Bedding Plants ◽

Normal Seedling ◽

First Results

The foremost factor necessary for plant growers cultivating large acreages of Stevia rebaudiana (Bert) Bertoni is the production of qualitative bedding plants. The objective of this study was to evaluate the influence of osmotic-priming on the uniformity of seed germination. First, we evaluated the percentage of normal seedlings from two seed samples harvested in 2011 and 2012. The seeds harvested in 2012 produced 71.4% normal seedlings and thus they were used in the next experiments. The seeds were subjected to osmotic stress using five concentrations of polyethylene glycol (PEG-6000) at -0.2, -0.4, -0.6, -0.8, and -1.0 MPa in contrast with distilled water. Based on these first results, only -0.8 and -1.0 MPa were evaluated in the third experiment. The seeds were immersed in both concentrations of polyethylene glycol (PEG-6000) for imbibing at 20ºC for four, five, six, and seven days. Thereafter, we evaluated the time to the first normal seedling (Ti), time to the last normal seedling (Tf), percentage normal seedlings at the initial time (Pi) and percentage of normal seedlings at the end of every treatment (Pf). Osmotic priming increased the percentage of normal seedlings of the Stevia rebaudiana and reduced the time to the first and last germination events.

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Effects of Different Priming Treatments on Seed Germination Properties, Yield Components and Grain Yield of Lentil (Lens culinaris Medik.)

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha4319832 ◽

2015 ◽

Vol 43 (1) ◽

pp. 153-158 ◽

Cited By ~ 2

Author(s):

Faruk TOKLU

Keyword(s):

Seed Germination ◽

Grain Yield ◽

Yield Components ◽

Germination Rate ◽

Seedling Emergence ◽

Yield Component ◽

Field Conditions ◽

Distilled Water ◽

Peg 6000 ◽

Positive Effects

An experiment was conducted under laboratory and field conditions in order to evaluate the effects of different priming treatments, specifically KNO3 (1%), KCl (2%), KH2PO4 (1%), ZnSO4 (0.05%), PEG-6000 (20%), IBA (100 ppm), Mannitol (4%), GA3 (100 ppm) and distilled water, on seed germination properties and several agro-morphological plant characteristics of red lentil. Seeds not primed were used as a control. GA3 treatment increased shoot length. The control (non-primed seeds) treatment resulted in increased seedling root number and length. Distilled water, ZnSO4 and control treatments increased germination rate and percentage. In the pot experiments, GA3 treatment increased plant height and seedling emergence rate, whereas KCl treatment improved the number of nodules, as well as root and shoot dry weight when compared to the control. ZnSO4 treatment increased yield components and grain yield in field conditions. The results of this study showed that ZnSO4, GA3 and PEG-6000 seed priming treatments may be useful tools due to their positive effects on germination rate, germination percentage, yield component and grain yield of lentil.

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Seed germination, hydrothermal time models and the effects of global warming on a threatened high Andean tree species

Seed Science Research ◽

10.1017/s0960258512000189 ◽

2012 ◽

Vol 22 (4) ◽

pp. 287-298 ◽

Cited By ~ 7

Author(s):

Edgar E. Gareca ◽

Filip Vandelook ◽

Milton Fernández ◽

Martin Hermy ◽

Olivier Honnay

Keyword(s):

Global Warming ◽

Seed Germination ◽

Water Availability ◽

Tree Species ◽

Hydrothermal Time ◽

Germination Process ◽

Germination Characteristics ◽

Wide Range ◽

Maximum Temperatures ◽

Insight Into

AbstractSeed germination is a crucial event in a plant's life cycle. Because temperature and water availability are important regulators of seed germination, this process will likely be influenced by global warming. Insight into the germination process under global warming is thus crucial, and requires the study of a wide range of water availability and temperature conditions. As hydrothermal time (HTT) models evaluate seed germination for any combination of water potential and temperature, they can be suitable to predict global warming effects on seed germination. We studied the germination characteristics of the high Andean endemic tree speciesPolylepis besseri(Rosaceae), using HTT models. We were especially interested in the potential effects of global warming on seed germination. Assembly of HTT models forP. besseriwas fairly straightforward due to the lack of a seed dormancy mechanism. The models allowed prediction ofPolylepisgermination under constant and alternating temperatures. Initially, a global warming induced increase in the field minimum and mean temperature will increaseP. besserigermination, but as maximum temperatures rise above the optimum temperature for the species, seed germination will become jeopardized. Effects of global warming on seed germination are currently considerably underexplored. HTT models prove to be useful tools to study a plant species' general germination characteristics, and how they may become affected under global warming. For the endemic mountain tree speciesP. besseri, we predict an increase, followed by a decrease of seed germination under global warming.

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Effects of osmotic priming on dormancy and storability of tomato (Lycopersicon esculentumMill.) seeds

Seed Science Research ◽

10.1017/s0960258500003020 ◽

1996 ◽

Vol 6 (2) ◽

pp. 49-55 ◽

Cited By ~ 22

Author(s):

Yongqing Liu ◽

R. J. Bino ◽

W. J. van der Burg ◽

S. P. C. Groot ◽

H. W. M. Hilhorst

Keyword(s):

Seed Germination ◽

Lycopersicon Esculentum ◽

Ambient Temperature ◽

Untreated Control ◽

Seed Priming ◽

Positive Effects ◽

Germination Process ◽

Controlled Deterioration ◽

Physical Changes ◽

Seed Storability

AbstractFreshly harvested tomato (Lycopersicon esculentumMill, cv. Moneymaker) seeds were osmotically primed for 8 d in −1.0 MPa PEG-6000 solution and dried to about 6% water content for storage. Such so–called ‘fresh PEG priming’ enhanced seed germination and improved seedling performance as compared with the untreated control. Fresh PEG priming neither alleviated seed dormancy nor promoted DNA replication as was the case when seeds were dried upon harvest and subsequently primed in PEG (normal PEG priming). However, the addition of 10 μM GA4+7to the osmotic priming solution triggered replicative DNA synthesis of fresh-priming seeds and further enhanced the germination process. After 5 months of storage in ambient temperature conditions, fresh PEG-primed seeds maintained more positive effects gained from priming, whereas, normal PEG-primed seeds had lost the promoting effects on germination. Normal PEG-primed seeds were much more susceptible to controlled deterioration than fresh PEG-primed seeds. It is suggested that the advancement of germination is negatively correlated with seed storability. The mechanisms of seed priming in relation to nuclear replication activities and physical changes are discussed.

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The Effect of Hormone Seed Priming Using Gibberellic Acid on Seed Germination Characteristics and Seedling Growth of Coneflower (Echinacea purpurea)

Iranian Journal of Seed Research ◽

10.52547/yujs.8.1.173 ◽

2021 ◽

Vol 8 (1) ◽

pp. 173-188

Author(s):

Farshid Yousefi ◽

Abdolreza Sihampoosh ◽

Abdolmahdi Bakhshandeh ◽

Seyyed Amir Mousavi ◽

◽

...

Keyword(s):

Seed Germination ◽

Gibberellic Acid ◽

Seedling Growth ◽

Seed Priming ◽

Echinacea Purpurea ◽

Germination Characteristics

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Ameliorative Effect of Seed Priming on Germination, Vigour Index and Tolerance Index against Short Term Moisture Stress in Maize (Zea mays L.)

Indian Journal of Agricultural Research ◽

10.18805/ijare.a-5351 ◽

2020 ◽

Author(s):

V. Singh ◽

M. Sharma ◽

H. Upadhyay ◽

A. Siddique

Keyword(s):

Polyethylene Glycol ◽

Seed Priming ◽

Moisture Stress ◽

Magnesium Nitrate ◽

Tolerance Index ◽

Short Term ◽

Seed Vigour ◽

Peg 6000 ◽

Vigour Index ◽

Polyethylene Glycol 6000

An experiment was carried out to evaluate the effect of seed priming with magnesium nitrate salt against the moisture stress condition induced by Polyethylene glycol-6000 on seed germination, vigour index and tolerance index in root and shoot. Polyethylene glycol-6000 was used to create short term moisture stress under laboratory conditions while the seed priming was carried out by using magnesium nitrate. The percent germination, seed vigour index and tolerance index were started to decline from T1 to T4 (i.e. set first 1.5 and 4.5 % of PEG-6000 solution + none primed seed) while the results of second set (i.e. PEG-6000 + primed seed from T5 to T8) were also following the same decreasing trend along with improvement in every parameters. The germination was recorded highest 100 percent in T5 (i.e. 1.5% PEG-6000+ primed seed) followed by T6, T1 and T7 (T6 and T7 is 2.5% and 3.5 % PEG-6000+ primed seed) while the control set recorded 98.7% at final date of observations i.e. 240 Hrs. vigour index and tolerance index was also decreasing in both the set of treatments but PEG-600 + primed seed showed better results as compared to the treatments PEG-6000 + none primed. Tolerance index percent was recorded maximum in root as compare to shoot at 120 hrs. of intervals while the reverse result was recorded at 240 hrs. of intervals.

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The Effect of Invigoration Using Polyethylene Glycol And Ultra Fine Bubble on Improving of Sengon Seeds (Falcataria Moluccana Miq.) Quality After Two Years Storage

Jurnal Wasian ◽

10.20886/jwas.v8i2.5997 ◽

2021 ◽

Vol 8 (2) ◽

pp. 133-143

Author(s):

Iskandar Siregar ◽

◽

Riki Ramdhani ◽

Evayusvita Rustam ◽

Dede Sudrajat ◽

...

Keyword(s):

Polyethylene Glycol ◽

Seed Germination ◽

Germination Rate ◽

Seed Viability ◽

Germination Capacity ◽

Peg 6000 ◽

Vigor Index ◽

Fine Bubble ◽

Initial Seed ◽

Environmental Air

Utilization of seeds storage results in decreased plant productivity. The increase can be used by treating seeds before planting through the technique of invigoration. This purpose of the study is to assess the effectiveness of invigoration methods using polyethylene glycol (PEG 6000) and ultrafine bubbles (UFB) to improve viability and vigor of sengon seeds (Falcataria moluccana). Seed agieng using 96 % ethanol was carried out to obtain the diversity of seedlot viability as the materials for testing the effectiveness of invigoration treatments. A complete random design was used to test the effectiveness of 5 invigoration treatments, i.e., seed without invigoration treatment, soaking in PEG 6000 -0.8 Mpa, soaking in PEG 6000 -1.2 Mpa, soaking in UFB water injected by environmental air, and soaking in UFB water injected by oxygen 99 %, with soaking time is 24 hours for each treatment. Seed agieng resulted three classes of seed viability, i.e. 62 % (initial seed), 83 % (seed agieng for 30 minutes) and 57 % (seed agieng for 60 minutes). In the condition of seed germination before treatment (DBA) 57 % and DBA 62%, invigoration treatments were significantly affected on seed germination capacity, but not significantly different in DBA 83 %. The soaking treatment of UFB injected by oxigen 99 % was able to improve the germination parameters (germination capacity, germination rate, and vigor index) on the sengon seeds with DBA 57 %. For seeds with DBA 62 %, the soaking treatment in UFB injected by environmental air was provided the best germination capacity, T50, and vigor index. The treatment of UFB injected by oxygen 99 % was more effective to improve the seed with very low viability and vigor (DBA 57 %). In general, improving of seed viability and vigor is more effective by using UFB than PEG 6000.

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Pattern of Capsule and Seed Development in Sesame (Sesamum indicum L.) as Influenced by Seed Priming

International Journal of Environment and Climate Change ◽

10.9734/ijecc/2020/v10i430189 ◽

2020 ◽

pp. 1-7

Author(s):

Utpal Biswas ◽

Rupa Das ◽

Amitava Dutta

Keyword(s):

Polyethylene Glycol ◽

Seed Development ◽

Seed Weight ◽

Seed Priming ◽

Quality Parameters ◽

Steady Increase ◽

Peg 6000 ◽

Yield And Quality ◽

Main Plot ◽

Days After Anthesis

Aim: To study the influence of seed priming on the pattern of capsule and seed development in sesame. Place and Duration of Study: The field experiment was conducted during the pre kharif seasons of 2017-2018 and 2018-2019 in sesame variety Savitri at AB Block farm, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, Nadia, West Bengal, India. Methodology: Experiment was laid out in split plot design with 3 replications. Ten schedules of seed priming viz T1 (KNO3 @ 10 mM), T2 (KNO3 @ 20 mM), T3 (KNO3 @ 50 mM), T4 (KH2PO4 @ 50 mM), T5 (KH2PO4 @ 100 mM),T6 (KH2PO4 @ 200 mM), T7 [Polyethylene glycol (PEG) 6000 @ -0.4 MPa], T8 [Polyethylene glycol (PEG) 6000 @ -0.3 MPa], T9 [Polyethylene glycol (PEG) 6000 @ -0.2 MPa], T10 Distilled water (Hydro priming) along with control T11 (Dry seed) were taken as main plot treatment and stage of harvest was considered as sub plot treatment. The pattern of capsule and seed development was studied at 10 days after anthesis (DAA), 20 days after anthesis (DAA), 30 days after anthesis (DAA), 40 days after anthesis (DAA) and 50 days after anthesis (DAA) interval. Ten plants from each replication and in each treatment were selected at random to record data on morphological and physiological characters. Results: Fresh capsule length, fresh capsule breadth, fresh capsule weight, fresh seed weight and dry seed weight showed a steady increase up to 40 days after anthesis (DAA) then decreased slowly up to maturity. Conclusion: Considering seed yield and quality parameters, T7 [Polyethylene glycol (PEG) 6000 @ -0.4 MPa] and T9 [Polyethylene glycol (PEG) 6000 @ -0.2 MPa] appears to be ideal among the treatments for quality seed production in sesame.

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