Gibberellic Acid Biosynthesis During Dehydration Phase of Priming Increases Seed Vigour of Tomato

2021 ◽  
Author(s):  
Seethalakshmi S ◽  
Umarani R ◽  
Djanaguiraman Maduraimuthu
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Seed Priming ◽  
Seedling Vigour ◽  
Seed Vigour ◽  
Seedling Traits ◽  
Physiological Processes ◽  
Germination Process ◽  
Priming Process ◽  
Subsequent Effect

Abstract Priming of seed is intended to reduce the time to germination through activation of pre-germinative processes. Seed priming is controlled hydration and followed by a drying (dehydration) process. The physiological processes during hydration (imbibition) were studied in detail in tomato. In contrast, gibberellic acid (GA) changes during the dehydration phase were not studied in detail. We hypothesize that there would be a change in the GA concentration during the dehydration phase of the seed priming process, which may influence the vigour characteristics of the resultant seedling. The objective was to understand the influence dehydration phase of seed priming on GA biosynthesis and its subsequent effect on seed germination and seedling traits of tomato. First, the hydroprimed and unprimed seeds were re-imbibed for 3, 6, 9, and 12 h to initiate the germination process, and the GA concentration and seedling vigour associated parameters were recorded. In the second experiment, the imbibed seeds were dehydrated for 3, 6, 9, and 12 h, then re-imbibed for 3, 6, 9, and 12 h to understand the effect of dehydration on the GA concentration and its associated traits. Results revealed that hydroprimed seeds had a higher GA concentration and seedling vigour than unprimed seeds. The seeds that are completely dehydrated for 12 h had the highest GA and seed vigour parameters. Therefore, increased vigour of hydroprimed seeds is due to the higher levels of GA accumulated during the dehydration phase of seed priming, which can improve seed germination and seedling vigour of tomato.

Micro-nutrients Seed Priming and rhizobium Coating on Seed Vigour, Crop Growth and Seed Yield in Blackgram (Vigna mungo L.)

2021 ◽  
Author(s):  
S. Kavitha ◽  
P. Srimathi
Keyword(s):  
Seed Germination ◽  
Seed Yield ◽  
Field Experiments ◽  
Seed Quality ◽  
Seed Priming ◽  
Quality Parameters ◽  
Crop Growth ◽  
Seedling Vigour ◽  
Seed Vigour ◽  
Vigour Index

Background: Black gram is the important pulses crop, grown throughout the country; gives low seed yield mainly due to poor management and low soil fertility. Development of suitable technology is essential to enhance productivity. Seed priming is one such effective technology which enhance rapid and uniform emergence to achieve high vigour, leading to better stand establishment and yield. The current research aimed to study micronutrient seed priming and rhizobium coating on seed germination, seedling vigour, crop growth and seed yield in black gram.Methods: Blackgram seeds were primed with 10 different micronutrient solutions with different concentrations and evaluated for its quality parameters to find out suitable seed priming technique. Seeds from two best priming treatments were coated with rhizobium @ 30g/kg of seed and evaluated for its production potential and resultant seed quality under field condition.Result: Seed priming with 0.5% ZnSO4 at 1/3rd seed to solution ratio for 3 hours recorded higher germination (96%) and vigour index (3811) than control which recorded 84% seed germination and 2528 of vigour index under laboratory experiment. Field experiments revealed that, seeds primed with 0.5% ZnSO4 and coated with rhizobium @ 30g per kg of seeds improved seed yield by 13.2% over control. 

scholarly journals EFFECT OF PRIMING WITH SILVER FIR AND OREGANO ESSENTIAL OILS ON SEED GERMINATION AND VIGOUR OF SILENE SENDTNERI

2021 ◽  
Vol 50 (1) ◽  
pp. 27-32
Author(s):  
Erna Karalija ◽  
Fatima Pustahija ◽  
Adisa Parić
Keyword(s):  
Essential Oils ◽  
Water Content ◽  
Germination Rate ◽  
Photosynthetic Pigment ◽  
Dry Weight ◽  
Seed Priming ◽  
Silver Fir ◽  
Seedling Vigour ◽  
Seed Vigour ◽  
Oregano Oil

UDK: 582.661.51:631.53.027]:547.913 In order to investigate the effects of seed priming with silver fir and oregano essential oils on certain important seedling characteristic and seed vigour of interesting endemic and horticultural species Silene sendtneri, an experiment was conducted based on randomized completely design with three replications. Traits such as germination rate, water content, dry weight, vigour index and photosynthetic pigments were analysed. Results revealed that the seedlings obtained with priming seeds showed increased growth, water content, vigour and photosynthetic pigment contents but decreased germination rate and dry weight compared with that obtained with non-primed seeds. Analysis of seed priming effects had demonstrated even germination rate is smaller the seedling vigour is slightly higher especially with all oregano oil treatments. We suggest that oregano oil has a potential as a priming agent for improvement of seedling synchrony, although at lower rate of germination.

Effects of osmotic priming on dormancy and storability of tomato (Lycopersicon esculentumMill.) seeds

1996 ◽  
Vol 6 (2) ◽  
pp. 49-55 ◽  
Author(s):  
Yongqing Liu ◽  
R. J. Bino ◽  
W. J. van der Burg ◽  
S. P. C. Groot ◽  
H. W. M. Hilhorst
Keyword(s):  
Seed Germination ◽  
Lycopersicon Esculentum ◽  
Ambient Temperature ◽  
Untreated Control ◽  
Seed Priming ◽  
Positive Effects ◽  
Germination Process ◽  
Controlled Deterioration ◽  
Physical Changes ◽  
Seed Storability

AbstractFreshly harvested tomato (Lycopersicon esculentumMill, cv. Moneymaker) seeds were osmotically primed for 8 d in −1.0 MPa PEG-6000 solution and dried to about 6% water content for storage. Such so–called ‘fresh PEG priming’ enhanced seed germination and improved seedling performance as compared with the untreated control. Fresh PEG priming neither alleviated seed dormancy nor promoted DNA replication as was the case when seeds were dried upon harvest and subsequently primed in PEG (normal PEG priming). However, the addition of 10 μM GA4+7to the osmotic priming solution triggered replicative DNA synthesis of fresh-priming seeds and further enhanced the germination process. After 5 months of storage in ambient temperature conditions, fresh PEG-primed seeds maintained more positive effects gained from priming, whereas, normal PEG-primed seeds had lost the promoting effects on germination. Normal PEG-primed seeds were much more susceptible to controlled deterioration than fresh PEG-primed seeds. It is suggested that the advancement of germination is negatively correlated with seed storability. The mechanisms of seed priming in relation to nuclear replication activities and physical changes are discussed.

Effect of Priming Techniques on Seed Germination Characteristics of C. Maxima Duch

2011 ◽  
Vol 474-476 ◽  
pp. 36-39 ◽  
Author(s):  
Yong Dong Sun ◽  
Xin Zheng Li ◽  
He Lian Yang ◽  
Li Sun
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Seed Priming ◽  
Distilled Water ◽  
Nacl Solution ◽  
Peg 6000 ◽  
Germination Process ◽  
Germination Characteristics ◽  
Successful Technique

The present study was conducted to investigate the effect of seed priming techniques on germination characteristics of C. maxima Duch. cultivar (Beiguan). Treatments were combinations of 3 levels of priming (distilled water, NaCl and PEG6000) and non-priming (control) with 3 replications. Concentrations of NaCl solution were 50, 100, 150, 200 mmol•L-1, and polyethylene glycol (PEG) 6000 were 10%, 20%, 30%, 40%, respectively. Seeds were primed using the above priming materials for 24 hours at 20°C in the dark, respectively. The results showed that different priming techniques could have various effects on germination of Beiguan seeds. Hydropriming (distilled water), NaCl priming and PEG6000 priming (10%) all improved the germination characteristics of Beiguan, compared to the control. NaCl priming was more effective than hydropriming and PEG6000 priming and was the most successful technique in this study. These findings indicated that seed priming techniques could accelerate germination process and were simple and cheap, we should propose these methods to farmers.

scholarly journals The Effect of Hormone Seed Priming Using Gibberellic Acid on Seed Germination Characteristics and Seedling Growth of Coneflower (Echinacea purpurea)

2021 ◽  
Vol 8 (1) ◽  
pp. 173-188
Author(s):  
Farshid Yousefi ◽  
Abdolreza Sihampoosh ◽  
Abdolmahdi Bakhshandeh ◽  
Seyyed Amir Mousavi ◽  
◽  
...  
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Seedling Growth ◽  
Seed Priming ◽  
Echinacea Purpurea ◽  
Germination Characteristics

Vigna unguiculata seed priming is related to redox status of plumule, radicle and cotyledons

2019 ◽  
Vol 46 (6) ◽  
pp. 584
Author(s):  
Lilya Boucelha ◽  
Réda Djebbar ◽  
Ouzna Abrous-Belbachir
Keyword(s):  
Seed Germination ◽  
Vigna Unguiculata ◽  
Antioxidant Activities ◽  
Seed Priming ◽  
Redox Status ◽  
Plant Production ◽  
Seed Vigour ◽  
Production Of Hydrogen ◽  
Metabolic Activities ◽  
The One

Pre-germination treatments represent the physiological methods that improve plant production by modulating the metabolic activities of germination before the emergence of the radicle. It was suggested that reactive oxygen species (ROS) play a crucial role in signalling seed germination. Our work consisted in studying changes in the redox status in the embryonic axis (radicle and plumule) and in cotyledons of Vigna unguiculata (L.) Walp. non-primed, osmoprimed (30% PEG6000), hydroprimed or twice hydroprimed seeds, by estimating antioxidant activities and production of ROS. Some antioxidant enzymatic activities as well as total non-enzymatic antioxidant capacity were measured. The production of hydrogen peroxide (H2O2) and superoxide anion (O2–) was also assessed by 3,3′-diaminobenzidine (DAB) and nitroblue tetrazolium (NBT) respectively. The results obtained showed, on the one hand, that priming allows activation of antioxidant enzymes, especially in the plumule. On the other hand, these results showed that priming caused an accumulation of ROS in embryonic tissues. This may explain the improvement of seed germination performance according to the oxidative window model. Priming induced changes in the redox environment at the seed level. These changes were closely related to the pre-germination treatments. Indeed, a double cycle of hydration-rehydration induced the broadest spectrum of modifications of the redox status, which would explain the improvement of the seed vigour.

Influence of Priming with Exogenous Selenium on Seed Vigour of Alfalfa (Medicago sativa L.)

2021 ◽  
Author(s):  
F.S. Xia ◽  
C.C. Wang ◽  
Y.Y. Li ◽  
Y.Y. Yang ◽  
C. Zheng ◽  
...  
Keyword(s):  
Higher Plants ◽  
Seed Priming ◽  
Germination Percentage ◽  
Health Concern ◽  
Global Public Health ◽  
Seedling Vigour ◽  
Seed Vigour ◽  
Vigour Index ◽  
Low Selenium ◽  
Alfalfa Seeds

Background: Selenium (Se) is an essential trace element to higher plants, animals and humans, but low Se levels are a global public health concern. Seed priming has become a basic strategy for the production of Se-riched agricultural products, but its application is still not clear in the production of Se-enrich alfalfa, hence this study was conducted for the production of Se-enriched alfalfa by seed priming with different concentrations and time on seed vigour. Methods: Seeds were primed with 0, 0.5, 1.0, 2.0, 4.0 and 8.0 mmol L-1 of sodium selenite solution for 0, 3, 6, 9 and 12 h at 20°C and their germination percentage, germination index, seedling vigour index and mean germination time were analyzed. Result: Seed vigour of alfalfa was improved by priming with low selenium (Se) concentration (0.5 and 1.0 mmol L-1), but was inhibited by high Se concentration ( greater than 2.0 mmol L-1). Hence, it must be necessary to carefully select appropriate concentration and time for the application of Se priming in alfalfa seeds. The optimal manner of Se priming in alfalfa seeds might be at 1.0 mmol L-1 concentration for 9 h.

scholarly journals Alleviate Seed Ageing Effects in Silybum marianum by Application of Hormone Seed Priming

2015 ◽  
Vol 7 (3) ◽  
pp. 316-321
Author(s):  
Seyed Ata SIADAT ◽  
Seyed Amir MOOSAVI ◽  
Mehran SHARAFIZADEH
Keyword(s):  
Seed Germination ◽  
Seed Quality ◽  
Morphological Structure ◽  
Seed Priming ◽  
Silybum Marianum ◽  
Duration Of Treatment ◽  
Seed Vigour ◽  
Germination Characteristics ◽  
Poor Seed ◽  
Seed Ageing

Most of the medicinal plants have seed dormancy or poor seed germination. This is due to their natural compounds or their morphological structure. Existence of such mechanisms makes the seeds able to endure harsh environments, stressful conditions or natural ageing. Different seed enhancement treatments were proposed in order to improve seed germination. In this study, it was examined Silybum marianum seed longevity (0, 48 and 72 hour of ageing) and the response to seed priming when using different concentrations of cytokinin (50, 200, 350 and 500 ppm) and different duration of treatment (8, 12 and 24 hour). Results revealed that ageing could be successfully alleviated using seed priming. Seed ageing significantly affected seed germination and vigour. Application of cytokinin considerably improved seed vigour in aged and non aged seeds. The most effective seed priming treatment was with 500 ppm cytokine, with the duration of 24 hours. In conclusion, it can be recommend the used of plant growth regulators like cytokine, as a good priming agent, to recover losses of seed quality and improve germination characteristics.

Ageing beautifully: can the benefits of seed priming be separated from a reduced lifespan trade-off?

2021 ◽  
Author(s):  
Isabelle Fabrissin ◽  
Naoto Sano ◽  
Mitsunori Seo ◽  
Helen M North
Keyword(s):  
Seed Priming ◽  
Ex Situ ◽  
Negative Effects ◽  
Seedling Vigour ◽  
Priming Effects ◽  
Seed Technology ◽  
Conflicting Evidence ◽  
Priming Process ◽  
Hydration And Dehydration

Abstract Germination performance is affected following seed exposure to a combination of temperature fluctuations and cycles of hydration and dehydration. This has long been exploited in a seed technology termed priming that increases germination speed and seedling vigour, but these benefits have often been associated with effects on seed lifespan, or longevity, with conflicting evidence for positive and negative effects. Seed longevity is a key seed trait influencing not only the storage of commercial stocks, but also in situ and ex situ seed conservation. In the context of increasingly variable environmental conditions faced by both crops and wild species, this has led to renewed interest towards understanding the molecular factors that underlie priming. Here, we give an overview of the literature relating to the effect of priming on seed lifespan and catalogue the different parameters used for priming treatments and their consequences on longevity for a range of species. Our current limited understanding of the molecular basis for priming effects is also outlined with an emphasis on recent advances and promising approaches that should lead towards applying and monitoring the priming process in a less empirical manner.

scholarly journals Seed and Seedling Vigour in Tropical Maize Inbred Lines

2014 ◽  
Vol 67 (1) ◽  
pp. 87-102
Author(s):  
M.A. Adebisi ◽  
T.O. Kehinde ◽  
J.B.O. Porbeni ◽  
O.A. Oduwaye ◽  
K. Biliaminu ◽  
...  
Keyword(s):  
Seed Germination ◽  
Inbred Lines ◽  
Shoot Length ◽  
Direct Selection ◽  
Tropical Maize ◽  
Seedling Vigour ◽  
Seed Vigour ◽  
Maize Inbred Lines ◽  
Group I ◽  
Vigour Index

Abstract Seed and seedling vigour is an aspect of seed quality which affects field establishment and performance. Low maize yields have been reported to be affected by several factors in which poor quality seed with low seed and seedling vigour. Information is scarce on seed and seedling vigour in maize inbred lines developed for tropical environments. Fifteen genotypes of tropical maize (Zea mays L.) inbred lines were evaluated to determine the level of differences in seed and seedling vigour traits, and extent of relationships among traits and their heritability. A substantial amount of genetic variability was found, which suggested that most of the traits under study could be improved through selection and utilized in breeding programs. Positive and significant interrelationship among seed germination and seedling traits and a positive correlation between seed germination and seed vigour and field emergence showed that these could be given due consideration in crop improvement for seed and seedling vigour. High genotypic coefficient of variation, heritability and genetic advance were obtained for seed germination, seed emergence, shoot length and seedling vigour index I and II, revealing the possibility of improvement in these characters through direct selection. The principal component analysis (PCA) identified seed emergence, shoot length, seedling vigour index I and II and tetrazolium vigour as characters that contributed greatly to variation in seed vigour in the maize inbred lines. Cluster analysis partitioned the genotypes into two groups, with group I consisting of seven genotypes and group II comprised the other eight genotypes, which suggested that hybridization between the two groups could lead to high level of heterosis. Genotype V5 had superior seedling vigour traits compared to other genotypes. Seedling emergence, shoot length, seedling vigour index I, seedling vigour index II and tetrazolium vigour are effective characters for good seedling vigour traits in maize inbred lines investigated.

Sign in / Sign up

Export Citation Format

Share Document