Ruminal fermentation, methanogenesis and nitrogen utilization of sheep receiving tropical grass hay-concentrate diets offered with Sapindus saponaria fruits and Cratylia argentea foliage

2004 ◽  
Vol 79 (1) ◽  
pp. 177-189 ◽  
Author(s):  
H. D. Hess ◽  
R. A. Beuret ◽  
M. Lötscher ◽  
I. K. Hindrichsen ◽  
A. Machmüller ◽  
...  
Keyword(s):  
Latin Square ◽  
Basal Diet ◽  
Open Circuit ◽  
Ruminal Fermentation ◽  
Body Protein ◽  
Protein Retention ◽  
Methane Release ◽  
Grass Hay ◽  
Sapindus Saponaria ◽  
Tropical Grass

AbstractThe effects of supplementing a tropical, low-quality grass hay (Brachiaria dictyoneura) with legume foliage (Cratylia argentea) or fruits of the multipurpose tree Sapindus saponaria on ruminal fermentation, methane release and nitrogen (N) utilization were evaluated. Six Swiss White Hill lambs were used in a 6 ✕ 6 Latin-square design with a 3 ✕ 2 factorial arrangement of treatments with measurements of energy metabolism being conducted using open-circuit respiratory chambers. Treatments consisted of three basal diets, either grass alone or legume: grass ratios of 1: 2 or 2: 1. These basal diets were supplemented (1: 3) with a control concentrate or with a concentrate containing 250 g/kg dry matter of S. saponaria fruits. The apparent total tract digestibilities of organic matter (OM) and neutral-detergent fibre (NDF) were reduced and the proportionate crude protein (CP) losses through faeces were increased (P 0·01) by supplementation with S. saponaria, and digestibilities of OM and NDF were linearly reduced (P 0·001) with increasing legume proportion. Body energy retention, however, was similar in all diets. Along with CP intake, the proportionate CP losses through faeces decreased (P 0·001) with increasing legume proportion which was associated with improved (P 0·001) body protein retention and reduced (P 0·1) fat retention. Ruminal fluid ammonia concentration was not significantly affected (P > 0·1) by the inclusion of S. saponaria in the concentrate, but increased linearly (P 0·001) as dietary legume proportion was elevated. Supplementation with fruits of S. saponaria increased (P 0·01) total bacteria count, and decreased (P 0·001) total ciliate protozoa count by more than proportionately 0·50. Daily methane release was reduced (P 0·01) by S. saponaria supplementation in all basal diet types. Although being not clearly affected on a daily basis, methane release relative to body protein retention decreased linearly (P 0·05) with increasing legume proportion. The fact that interactions were mostly non-significant (P > 0·05) indicates that supplementation with S. saponaria fruits is a useful means to reduce methane emission from sheep given both tropical grass-based and grass-legume-based diets. Likewise, including legumes in N-limited tropical diets seems to represent an environmentally friendly way to improve animal productivity.

scholarly journals Partial substitution of fish oil for linseed oil enhances beneficial fatty acids from rumen biohydrogenation but reduces ruminal fermentation and digestibility in growing goats

2021 ◽  
Author(s):  
Lam Phuoc Thanh ◽  
Noppharat Phakachoed ◽  
Wisitiporn Suksombat ◽  
Juan J Loor ◽  
Tran Thi Thuy Hang
Keyword(s):  
Fish Oil ◽  
Linseed Oil ◽  
Latin Square ◽  
Basal Diet ◽  
Partial Substitution ◽  
Partial Replacement ◽  
Minimal Risk ◽  
Ruminal Fermentation ◽  
Growing Goats

Abstract This study was performed to investigate effects of partial replacement of fish oil (FO) for linseed oil (LO) on digestibility, ruminal fermentation and biohydrogenation in growing goats. Experiment 1 was carried out in four growing male goats aged 6 months in a 4 × 4 Latin square design. Goats were fed a basal diet supplemented with 25 g/kg dry matter either LO alone or in combination with tuna FO. Treatments were developed by replacing FO for LO at ratios of 0, 5, 10 and 15 g/kg DM corresponding to FO-0, FO-5, FO-10 and FO-15, respectively. Experiment 2 was carried out in an in vitro incubation system including 12 fermenters with the same four treatments. Each fermenter consisted of 40 mL goat ruminal fluid, 160 mL warm buffer, 2 g mixed substrates, and 50 mg FO-0, FO-5, FO-10 or FO-15. Fish oil inclusion reduced (P < 0.05) digestibility and nitrogen retention in Exp. 1. Increasing doses of FO in the diet induced a strong drop (P < 0.001) in ruminal total volatile fatty acid (VFA) concentration and protozoa population at 3 h post incubation, but did not affect individual VFA proportions. Substitution of FO for LO decreased mean concentrations of C18:0 (P = 0.057), c-9,c-12 C18:2 and C18:3n-3 (P < 0.001), but increased (P < 0.001) C20:5n-3 and C22:6n-3. Feeding FO-10 enhanced formation of ruminal c-9,t-11 conjugated linoleic acid (CLA) concentration compared with FO-0. Overall, combined data suggest that to improve ruminal concentrations of C20:5n-3, C22:6n-3, and c-9,t-11 CLA for deposition in tissues or milk with minimal risk of affecting digestibility and ruminal fermentation, a dietary supplementation of 15 g/kg LO and 10 g/kg FO would be suitable.

Effect of Tween 80 and salinomycin on ruminal fermentation and nutrient digestion in steers fed a diet containing 70% barley

2000 ◽  
Vol 80 (2) ◽  
pp. 363-372 ◽  
Author(s):  
A. N. Hristov ◽  
T. A. McAllister ◽  
M. E. Olson ◽  
K.-J. Cheng ◽  
L. J. Yanke ◽  
...  
Keyword(s):  
Volatile Fatty Acids ◽  
Latin Square ◽  
Fluid Phase ◽  
Tween 80 ◽  
Basal Diet ◽  
Barley Grain ◽  
Nutrient Digestibility ◽  
Ruminal Fermentation ◽  
Acid Detergent Fibre ◽  
Alfalfa Silage

Ten Jersey steers (484 ± 55 kg) were fed a basal diet of 70% rolled barley grain and 30% alfalfa silage [dry matter (DM) basis] with or without additives, in a replicated 5 × 5 Latin square experiment. Treatments (additives per kilogram dietary DM) were: C, no additives (control); T, Tween 80 (2 g kg−1); SH, salinomycin (13 mg kg−1); TSM, Tween 80 (2 g kg−1) plus salinomycin (6.5 mg kg−1); and TSL, Tween 80 (2 g kg−1) plus salinomycin (3.25 mg kg−1). Ruminal pH, concentrations of ammonia, total free amino acids, reducing sugars and total volatile fatty acids (VFA), and fluid phase viscosity were unaffected (P > 0.05) by treatment. Ruminal carboxymethylcellulase, xylanase and amylase activities, numbers of protozoa, and outflow rates of the liquid and solid phases of ruminal contents did not differ (P > 0.05) among treatments. Ruminal lactic acid bacteria populations tended (P < 0.1) to be smaller with SH compared with C, but counts were unaffected (P > 0.05) by T, TSL and TSM. Partitioning of ruminal digesta and microbial protein flow were similar (P > 0.05) among treatments. Compared with C, the ruminal rate of degradation (in situ) of alfalfa DM tended to be higher (P < 0.10) with SH, and the rate of barley grain DM degradation was higher (P < 0.05) with T. Intake and apparent digestibilities of DM, neutral detergent fibre (NDF), acid detergent fibre (ADF) and crude protein were unaffected (P > 0.05) by treatment, as were blood glucose and urea levels. Tween 80 and salinomycin did not affect ruminal fermentation or nutrient digestibility in steers fed a barley grain/alfalfa silage diet. Key words: Tween 80, salinomycin, ruminal fermentation, digestibility, steer

scholarly journals Effects of xylanase supplementation on feed intake, digestibility and ruminal fermentation in Rambouillet sheep

2016 ◽  
Vol 154 (6) ◽  
pp. 1110-1117 ◽  
Author(s):  
L. H. VALLEJO ◽  
A. Z. M. SALEM ◽  
L. M. CAMACHO ◽  
A. M. KHOLIF ◽  
M. D. MARIEZCURRENA ◽  
...  
Keyword(s):  
Feed Intake ◽  
Latin Square ◽  
Basal Diet ◽  
Experimental Period ◽  
Blood Parameters ◽  
Ruminal Fermentation ◽  
Maize Stover ◽  
Post Feeding ◽  
Feed Digestibility ◽  
Xylanase Enzyme

SUMMARYThe present study aimed to investigate the effects of adding xylanase enzyme (XY) to a basal diet containing 300 g maize stover and 700 g concentrate/kg dry matter (DM) on feed intake, ruminal fermentation, total tract and ruminal digestibility, as well as some blood parameters. Four male Rambouillet sheep (39 ± 1·8 kg body weight), with permanent rumen and duodenum cannulae were used in a 4 × 4 Latin square design. Sheep were fed a basal diet without xylanase addition (control, XY0), or with the addition of xylanase at 1 (XY1), 3 (XY3) or 6 (XY6) μl/g of diet DM for 84 days, with four 21-day experimental periods. Feed intake, digestibility and rumen fermentation parameters were determined on days 16–21 in each experimental period, and the apparent ruminal neutral detergent fibre (NDF) digestibility was determined on days 16 and 17. Treatments XY1 and XY3 increased feed intake, whereas digestibility was increased with XY6. Ruminal NDF digestibility increased when sheep were fed diets treated with xylanase. Ruminal pH, ammonia-N and acetic acid increased with xylanase treated diets. Propionic acid concentration increased with diet XY1 at 3 h post-feeding, but after 9 h post-feeding its concentration decreased in the rumen of sheep fed xylanase treated diets. Xylanase had no effect on blood urea, phosphorus and triglycerides. Addition of xylanase at 6 µl/g DM in a diet containing 300 g maize stover and 700 g concentrate/kg DM and fed to Rambouillet sheep improved feed digestibility and ruminal fermentation without affecting blood parameters.

scholarly journals PSXII-20 Effects of association between monensin, calcium nitrate and tannin on enteric methane production in cannulated beef cattle

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 420-421
Author(s):  
Alice H Assumpçao ◽  
Cristiane Tropaldi ◽  
Guilherme Sene ◽  
Ramos Tseu ◽  
Flávio Perna Junior ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Methane Production ◽  
Calcium Nitrate ◽  
Latin Square ◽  
Basal Diet ◽  
Feed Additives ◽  
Ex Situ ◽  
Experimental Unit ◽  
Ruminal Fermentation ◽  
Ch4 Production

Abstract The objective of this experiment was to evaluate the association between different feed additives on ruminal fermentation parameters especially ruminal methane on cannulated beef cattle. Five Nellore cows, 480 ± 55 kg, ruminally cannulated were used. The experimental was a 5 x 5 Latin square design and the experimental unit was the animal within each period (n = 25 experimental units). The diets offered differed only by the inclusion or not of monensin, calcium nitrate or tannin, totaling five treatments: Control (without addition of additives), Monensin (30 ppm in the diet), Nitrate (3.0% of DM), Tannin (1.5% of DM) and Pool (combination of the three additives), being supplied in a basal diet with a concentrate: roughage (corn silage) ratio of 60:40. The study was divided into five periods, each one with 26 days. Sixteen days were used for adaptation to the experimental diets; ruminal fluid samples were collected at 0, 3, 6, 9 and 12h after feeding at 22nd day. To evaluate CH4 production and ammoniacal nitrogen balance, samples were incubated ex situ on equality ruminal conditions and then analyzed by gas chromatography and UV spectrophotometric, respectively. Data were analyzed using PROC MIXED of SAS and differences were declared significant at 5%. There was interaction between sampling time and treatment (P &lt; 0001) and all the additives were efficient in decreasing the methane production in mol/g/hour (P &lt; 0001), mol/kg/day (P &lt; 0001) and g/kg/day (P &lt; 0001). Comparing to the Control all additives decreased CH4 production, and decreases were 9.5% for monensin, 18.75% for tannin, 19.8% for calcium nitrate and 28.8% for the Pool (monensin, nitrate and tannin). Concentration and balance of rumen NH3-N was not affected by any treatments. It was possible to conclude that association of additives of different mechanism of action presented a partial additive effect.

scholarly journals Effects of essential oils of Cashew and Castor on intake, digestibility, ruminal fermentation and purine derivatives in beef cattle fed high grain diets

2019 ◽  
Vol 40 (5) ◽  
pp. 2057 ◽  
Author(s):  
Sabrina Marcantonio Coneglian ◽  
Roman David Castañeda Serrano ◽  
Olga Teresa Barreto Cruz ◽  
Antonio Ferriani Branco
Keyword(s):  
Beef Cattle ◽  
Essential Oils ◽  
Crude Protein ◽  
Ricinus Communis ◽  
Latin Square ◽  
Basal Diet ◽  
Corn Silage ◽  
Ruminal Fermentation ◽  
Purine Derivatives ◽  
Ruminal Ph

The objective of this study was to determine the effects of essential oils of Anacardium occidentale (Cashew) and Ricinus communis (Castor) on intake, digestibility, ruminal fermentation and excretion of purine derivatives in beef cattle fed high grain diets. Five Nellore steers fitted with ruminal cannula were used in a 5x5 Latin square design (21 days period). The treatments were control MON (0.2 g monensin animal day-1¬) and 1, 2, 4 and 8 g EO animal day-1 (Essential oils - Oligobasics®). All the animals had a basal diet, corn silage-based total mixed ration (TMR) of 80:20 concentrate:forage ratio. Intake, digestibility, ruminal fermentation and excretion of purine derivatives were determined over five consecutive days in each period. Intake and digestibility of dry matter (DM), neutral detergent fibre (NDF) and crude protein (CP) were not influenced by treatments (P > 0.05). However, increasing levels of EO showed a quadratic effect (P < 0.014) above 2 g animal day-1 on the ruminal pH. The excretion of allantoin and uric acid were not influenced by the treatments (P > 0.05), but levels above 2 g day decreased the synthesis of microbial proteins (P < 0.05). It is concluded that the EO of A. occidentale and R. comunnis effectively controlled ruminal fermentation as well as sodium monensin at the studied levels. EOs have the potential to be used in place of monensin in the studied levels.

Effects of Sapindus saponaria fruits on ruminal fermentation and duodenal nitrogen flow of sheep fed a tropical grass diet with and without legume1

2004 ◽  
Vol 82 (5) ◽  
pp. 1392-1400 ◽  
Author(s):  
A. Abreu ◽  
J. E. Carulla ◽  
C. E. Lascano ◽  
T. E. Díaz ◽  
M. Kreuzer ◽  
...  
Keyword(s):  
Nitrogen Flow ◽  
Ruminal Fermentation ◽  
Sapindus Saponaria ◽  
Tropical Grass

scholarly journals PSII-6 Comparative effects of two multi-species direct-fed microbial products on rumen fermentation, bacterial community and metabolome of beef steers

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 398-398
Author(s):  
Ibukun M Ogunade ◽  
Devan M Compart ◽  
Andres A Pech-Cervantes
Keyword(s):  
Bacillus Subtilis ◽  
Bacterial Community ◽  
Relative Abundance ◽  
Isotope Labeling ◽  
Latin Square ◽  
Basal Diet ◽  
Beef Steers ◽  
Ruminal Fermentation ◽  
Metabolome Analysis ◽  
Fermentation Products

Abstract We examined the effects of two direct-fed microbials (DFM) containing multiple microbial species and their fermentation products on ruminal fermentation, bacterial community and metabolome of beef steers. Nine rumen-cannulated Holstein steers were assigned to 3 treatments arranged in a 3 × 3 Latin square design with three 21-d periods. Dietary treatments were (1) CON (basal diet without additive), (2) PROB (basal diet plus 19 g/d of Commence), and (3) SYNB (basal diet plus 28 g/d of RX3). Commence is a blend of active S. cerevisiae, Enterococcus lactis, Bacillus subtilis, Enterococcus faecium, and L. casei, and their fermentation products. RX3 is a blend of active S. cerevisiae and the fermentation products of S. cerevisiae, Enterococcus lactis, Bacillus licheniformis, and Bacillus subtilis. Daily dry matter intake was calculated. On d 21 of each period, ruminal contents were sampled at 3, 6, 12, and 18 h after feeding for analysis of VFA, bacterial community via 16S rRNA sequencing, and metabolome via chemical isotope labeling liquid chromatography mass spectrometry. There was no effect on DMI. Compared to CON, supplementation of either PROB or SYNB increased or tended to increase (P ≤ 0.10) total VFA, propionate, and valerate concentrations. Compared to CON, PROB reduced (P ≤ 0.05) the relative abundance of Prevotella 1 and Prevotellaceae UCG-001, but increased those of Rikenellaceae RC9, Succinivibrionaceae UCG-001, Succiniclasticum, Ruminococcaceae UCG-014, and Ruminococcaceae UCG-002, whereas SYNB decreased (P ≤ 0.05) the relative abundance of Prevotella 1 and Prevotellaceae UCG-001, and increased those of Prevotella 7, Succinivibrio, Succiniclasticum, and Ruminococcaceae UCG-014. Metabolome analysis revealed that 8 ruminal metabolites, including some amino acids, were increased (P ≤ 0.05) by PROB, whereas no differences were found for SYNB. This study demonstrated that supplemental PROB or SYNB altered the ruminal bacterial community and metabolome differently to achieve a similar ruminal fermentation pattern.

Methane emissions from sheep fed Eragrostis curvula hay substituted with Lespedeza cuneata

2020 ◽  
Vol 60 (15) ◽  
pp. 1777
Author(s):  
C. J. L. du Toit ◽  
W. A. van Niekerk ◽  
H. H. Meissner ◽  
L. J. Erasmus ◽  
R. J. Coertze
Keyword(s):  
Production Systems ◽  
Latin Square ◽  
Basal Diet ◽  
Open Circuit ◽  
Adaptation Period ◽  
Lespedeza Cuneata ◽  
Eragrostis Curvula ◽  
Ch4 Emissions ◽  
Experimental Treatments ◽  
Measurement Period

Context Reducing emissions of greenhouse gases from livestock production systems is a global research priority. Forages that contain condensed tannins, such as the perennial legume Lespedeza cuneata, may help to reduce ruminant methane (CH4) emissions. Aims The objective of this study was to investigate the effect of feeding different levels of L. cuneata hay on feed intake and enteric CH4 emissions of sheep fed a basal diet of subtropical Eragrostis curvula hay. Methods Four adult ruminally cannulated Dohne Merino wethers with initial bodyweight of 65.5 ± 3.5 kg were used in the experiment in a 4 × 4 Latin square design. The four experimental treatments were E. curvula hay substituted with 0%, 30%, 60% and 90% L. cuneata hay. Each of four experimental periods lasted 27 days, which consisted of a 14-day adaptation period, a 7-day digestibility trial, and a 6-day CH4-measurement period. During the 6-day CH4-measurement period, CH4 emissions were measured continuously over a 24-h period by using an open circuit respiration system. Key results Dry matter intake (DMI, g/kg W0.75) was higher (P &lt; 0.05) for sheep receiving 60% and 90% L. cuneata than 0% and 30% L. cuneata (77.33 and 84.67 g/kg W0.75 vs 62.96 and 62.71 g/kg W0.75). The increase in DMI corresponded with a linear increase in DM digestibility of the experimental treatments from 38% to 45% as the level of L. cuneata substitution increased. Methane yield was not influenced (P &gt; 0.05) by 30% inclusion of L. cuneata (17.6 g CH4/kg DMI) but decreased (P &lt; 0.05) as the inclusion level increased to 60% and 90% (13.8 and 14.3 g CH4/kg DMI). Conclusions Inclusion of L. cuneata hay in a diet based on E. curvula hay improved diet digestibility, and led to increased concentrations of crude protein, neutral detergent fibre and non-fibre carbohydrates. Substituting E. curvula hay with 60% L. cuneata on a DM basis resulted in the greatest reduction in CH4 yield of 21.4% compared with a diet of 100% E. curvula. Implications The results suggest that L. cuneata has the potential to reduce CH4 yield and possibly increase production from sheep by improving diet DM digestibility and through improved DMI.

scholarly journals PSXIII-34 Effect of supplementation with corn DDGS on intake and ruminal fermentation parameters by beef steers fed a tropical grass hay.

2018 ◽  
Vol 96 (suppl_3) ◽  
pp. 437-438
Author(s):  
O Hernández ◽  
A López ◽  
M Nazareno ◽  
M García ◽  
P Reineri ◽  
...  
Keyword(s):  
Beef Steers ◽  
Ruminal Fermentation ◽  
Grass Hay ◽  
Fermentation Parameters ◽  
Tropical Grass ◽  
Corn Ddgs

Influence of zeolite (clinoptilolite) supplementation on characteristics of digestion and ruminal fermentation of steers fed a steam-flaked corn-based finishing diet

2018 ◽  
Vol 58 (7) ◽  
pp. 1239
Author(s):  
J. D. Urías-Estrada ◽  
M. A. López-Soto ◽  
A. Barreras ◽  
J. A. Aguilar-Hernández ◽  
V. M. González-Vizcarra ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Positive Impact ◽  
Latin Square ◽  
Basal Diet ◽  
Ruminal Fermentation ◽  
Faecal Excretion ◽  
Linear Effect ◽  
Molar Proportion ◽  
Volatile Fatty Acid Concentration

Four Holstein steers (328 ± 14 kg) with ruminal and duodenal cannulas were used in a 4 × 4 Latin square design to examine the effect of level of zeolite supplementation (0, 10, 20 or 30 g clinoptilolite-Ca/kg of diet) in a steam-flaked corn-based finishing diet on the characteristics of ruminal fermentation and nutrient digestion. Zeolite was top-dressed with the basal diet at time of feeding; therefore, intake of organic matter (OM), neutral detergent fibre (NDF), starch and N was equal in all treatments. Zeolite supplementation did not affect site and extent of digestion of N or NDF. Zeolite linearly decreased (P < 0.01) NH3-N flow to duodenum, but did not affect duodenal flow of microbial N, feed N, or microbial efficiency. Inclusion of zeolite linearly increased (P = 0.02) ruminal OM digestion and tended (linear, P = 0.08) to increase ruminal digestion of starch. Zeolite supplementation increased (linear effect, P < 0.01) faecal excretion of ash, resulting in decreased total tract DM digestion (linear effect, P = 0.04). However, total tract digestion of OM tended to increase (P = 0.06) with increasing zeolite supplementation. Thus, dilution of the diet with zeolite did not depress dietary digestible energy. There were no treatment effects on ruminal pH. Total ruminal volatile fatty acid concentration tended (P = 0.08) to increase as zeolite increased. Zeolite supplementation decreased ruminal molar proportion of acetate and increased molar proportion of propionate (linear effect, P < 0.01), resulting in decreased (linear effect, P < 0.01) of acetate: propionate ratio. Supplementation with zeolite at levels of up to 30 g of zeolite/kg of diet may enhance ruminal OM digestion, largely due to the tendency of increased ruminal starch digestion. Zeolite supplementation altered ruminal volatile fatty acid molar proportions (decreased acetate : propionate ratio), and therefore may have an positive impact on energy efficiency.

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