Application of a Validated Method for the Determination of Provitamin A Carotenoids in Indonesian Foods of Different Maturity and Origin

1997 ◽  
Vol 45 (4) ◽  
pp. 1174-1179 ◽  
Author(s):  
Paul J. M. Hulshof ◽  
Chao Xu ◽  
Peter van de Bovenkamp ◽  
Muhilal ◽  
Clive E. West
Keyword(s):  
Provitamin A ◽  
Provitamin A Carotenoids

Vitamin A Value of Plant Food Provitamin A - Evaluated by the Stable Isotope Technologies

2014 ◽  
Vol 84 (Supplement 1) ◽  
pp. 25-29 ◽  
Author(s):  
Guangwen Tang
Keyword(s):  
Stable Isotope ◽  
Vitamin A ◽  
Provitamin A ◽  
Animal Origin ◽  
Plant Food ◽  
Plant Foods ◽  
A Value ◽  
Provitamin A Carotenoids ◽  
Β Carotene

Humans need vitamin A and obtain essential vitamin A by conversion of plant foods rich in provitamin A and/or absorption of preformed vitamin A from foods of animal origin. The determination of the vitamin A value of plant foods rich in provitamin A is important but has challenges. The aim of this paper is to review the progress over last 80 years following the discovery on the conversion of β-carotene to vitamin A and the various techniques including stable isotope technologies that have been developed to determine vitamin A values of plant provitamin A (mainly β-carotene). These include applications from using radioactive β-carotene and vitamin A, depletion-repletion with vitamin A and β-carotene, and measuring postprandial chylomicron fractions after feeding a β-carotene rich diet, to using stable isotopes as tracers to follow the absorption and conversion of plant food provitamin A carotenoids (mainly β-carotene) in humans. These approaches have greatly promoted our understanding of the absorption and conversion of β-carotene to vitamin A. Stable isotope labeled plant foods are useful for determining the overall bioavailability of provitamin A carotenoids from specific foods. Locally obtained plant foods can provide vitamin A and prevent deficiency of vitamin A, a remaining worldwide concern.

scholarly journals Carotenoid Composition of Cionosicyos macranthus Fruit

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986264 ◽  
Author(s):  
Enrique Murillo ◽  
Moises Watts ◽  
Gabriel Reyna ◽  
Daniele Giuffrida ◽  
Armando A. Durant-Archibold
Keyword(s):  
Provitamin A ◽  
Total Carotenoid ◽  
Good Source ◽  
Provitamin A Carotenoids ◽  
Complete Determination ◽  
Carotenoid Composition ◽  
First Time ◽  
Derivatives Of ◽  
Edible Parts

A complete determination of the carotenoid composition of the edible aril and mesocarp of Cionosicyos macranthus fruit is reported for the first time. The carotenoids present in the fruit were identified and quantified using high-purity carotenoid standards. The fruit contained several rare carotenoids like zeaxanthin, β-cryptoxanthin, and cryptocapsin epoxides. Various keto-κ end-ring carotenoids, derivatives of their corresponding epoxides, ie cryptocapsin, capsanthin, capsanthin 5,6-epoxide, and capsoneoxanthin, were also identified. The total carotenoid contents for the aril and mesocarp were 226.0 μg/g and 83.4 µg/g, respectively. β-Cryptoxanthin was the most abundant carotenoid in both edible parts (58.3 μg/g in the aril and 29.5 μg/g in the mesocarp). Cryptocapsin was the primary keto-κ end-ring carotenoid both in the aril (41.6 μg/g) and in the mesocarp (13.2 μg/g). The fruit provitamin A activity was also determined. Considering the high β-cryptoxanthin and cryptocapsin contents, C. macranthus can be considered a good source of provitamin A carotenoids.

A Specific Method for the Determination of Provitamin A Carotenoids in Orange Juice

1975 ◽  
Vol 58 (3) ◽  
pp. 595-598 ◽  
Author(s):  
Samuel K Reeder ◽  
Gary L Park
Keyword(s):  
Liquid Chromatography ◽  
Orange Juice ◽  
High Speed ◽  
Provitamin A ◽  
Specific Method ◽  
Limits Of Detection ◽  
Provitamin A Carotenoids ◽  
Β Carotene

Abstract A method has been developed for rapidly determining the amounts of ∝-carotene, β-carotene, and cryptoxanthin in orange juice. The procedure includes extraction, saponification, and high-speed liquid chromatography. Limits of detection for the 3 carotenoids are 0.04, 0.02, and 0.04 μg/ml, respectively.

Determination of Carotenoids in Yellow Maize, the Effects of Saponification and Food Preparations

2008 ◽  
Vol 78 (3) ◽  
pp. 112-120 ◽  
Author(s):  
Muzhingi ◽  
Yeum ◽  
Russell ◽  
Johnson ◽  
Qin ◽  
...  
Keyword(s):  
High Performance ◽  
Provitamin A ◽  
Carotenoid Content ◽  
Carotenoid Concentration ◽  
Eye Health ◽  
Provitamin A Carotenoids ◽  
C30 Column ◽  
Β Carotene ◽  
Food Consumed

Maize is an important staple food consumed by millions of people in many countries. Yellow maize naturally contains carotenoids which not only provide provitamin A carotenoids but also xanthophylls, which are known to be important for eye health. This study was aimed at 1) evaluating the effect of saponification during extraction of yellow maize carotenoids, 2) determining the major carotenoids in 36 genotypes of yellow maize by high-performance liquid chromatography with a C30 column, and 3) determining the effect of cooking on the carotenoid content of yellow maize. The major carotenoids in yellow maize were identified as all-trans lutein, cis-isomers of lutein, all-trans zeaxanthin, α- and β-cryptoxanthin, all-trans β-carotene, 9-cis β-carotene, and 13-cis β-carotene. Our results indicated that carotenoid extraction without saponification showed a significantly higher yield than that obtained using saponification. Results of the current study indicate that yellow maize is a good source of provitamin A carotenoids and xanthophylls. Cooking by boiling yellow maize at 100° C for 30 minutes increased the carotenoid concentration, while baking at 450° F for 25 minutes decreased the carotenoid concentrations by almost 70% as compared to the uncooked yellow maize flour.

High Performance Liquid Chromatographic Determination of Provitamin A in Orange Juice

1977 ◽  
Vol 60 (1) ◽  
pp. 132-136 ◽  
Author(s):  
Ivan Stewart
Keyword(s):  
Orange Juice ◽  
High Performance ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Provitamin A ◽  
Provitamin A Carotenoids ◽  
Single Column ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A quantitative method has been developed for the determination of α- and β-carotenes and β-cryptoxanthin, the provitamin A carotenoids in orange juice. The carotenoids were separated by high performance liquid chromatography on a single column in approximately 30 min. The procedure may also be used to measure zeta-carotene and α-cryptoxanthin.

scholarly journals CD36 and SR-BI Are Involved in Cellular Uptake of Provitamin A Carotenoids by Caco-2 and HEK Cells, and Some of Their Genetic Variants Are Associated with Plasma Concentrations of These Micronutrients in Humans

2013 ◽  
Vol 143 (4) ◽  
pp. 448-456 ◽  
Author(s):  
Patrick Borel ◽  
Georg Lietz ◽  
Aurélie Goncalves ◽  
Fabien Szabo de Edelenyi ◽  
Sophie Lecompte ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Genetic Variants ◽  
Plasma Concentrations ◽  
Provitamin A ◽  
Provitamin A Carotenoids ◽  
Hek Cells

Vitamin A and the provitamin A carotenoids

1998 ◽  
pp. 115-161
Author(s):  
G. F. M. Ball
Keyword(s):  
Vitamin A ◽  
Provitamin A ◽  
Provitamin A Carotenoids

scholarly journals Content and Retention of Provitamin A Carotenoids Following Ripening and Local Processing of Four Popular Musa Cultivars from Eastern Democratic Republic of Congo

2012 ◽  
Vol 2 (2) ◽  
pp. 60 ◽  
Author(s):  
Beatrice Nakhauka Ekesa ◽  
Judith Kimiywe ◽  
Inge Van den Bergh ◽  
Guy Blomme ◽  
Claudie Dhuique-Mayer ◽  
...  
Keyword(s):  
Vitamin A ◽  
Palm Oil ◽  
Democratic Republic ◽  
Democratic Republic Of Congo ◽  
Vitamin A Deficiency ◽  
Provitamin A ◽  
Local Processing ◽  
Dietary Intakes ◽  
Republic Of Congo ◽  
Provitamin A Carotenoids

<p>Changes in the concentrations and retention levels of total and individual provitamin A carotenoids (pVACs) during ripening and local processing of the four most popular <em>Musa</em> cultivars of Eastern Democratic Republic of Congo were established through HPLC analysis. The predominant pVACs were all <em>trans</em> ?- and ?-carotene, together constituting about 90% of total pVACs content in raw and processed <em>Musa</em> fruit pulp. The proportion of ?- and ?-carotene was not significantly different in the tested East African Highland Bananas (AAA-EAHB) (‘Nshikazi’ and ‘Vulambya’); in the plantains (‘Musilongo’ and ‘Musheba’), proportion of ?-carotene was almost twice that of ?-carotene. An increase in total pVACs was observed during ripening, with highest levels at ripening stage 3 in all four cultivars. Total pVACs values were as high as 1081µg/100gfw in ‘Vulambya’ and 1819µg/100gfw in ‘Musilongo’. Boiling of the AAA-EAHB and AAB-Plantains resulted to retention of between 40-90% and &gt;95% respectively. Plantains deep-fried in fully-refined palm oil and crude red palm oil for 2 minutes did not seem to lose any pVACs, the levels of total pVACs observed after frying were 100% of what was observed when the fruit was raw. Retinol Activity Equivalents (RAE), in boiled products varied between 22.3 and 173 RAEµg/100gfw, whereas deep fried products had &gt;190 RAEµg/100g edible portion. These results show that the tested AAA-EAHBs and the plantains could meet at least 14% and 30 % of Vitamin A recommended dietary intakes respectively. The findings can therefore guide consumer consumption patterns to maximize vitamin A intake for improved health in these regions and also direct researchers in the selection of <em>Musa</em> cultivars to be incorporated within existing farming systems in the fight against vitamin A deficiency (VAD).</p>

scholarly journals Climate Change Enhanced Carotenoid Pro-Vitamin A Levels of Selected Plantain Cultivars

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 541 ◽  
Author(s):  
Beloved Mensah Dzomeku ◽  
Julian P. Wald ◽  
Jens Norbert Wünsche ◽  
Donatus Nohr ◽  
Hans K. Biesalski
Keyword(s):  
Low Cost ◽  
Dry Season ◽  
Provitamin A ◽  
Poor People ◽  
Carotene Content ◽  
Uv Index ◽  
Provitamin A Carotenoids ◽  
Micronutrient Intake ◽  
Freeze Dried ◽  
Β Carotene

Diet diversification and the exploitation of traditional, micronutrient-rich germplasm of staple crops are generally regarded as sustainable and low-cost approaches to increase the micronutrient intake of resource-poor people. Sun’s UV index was collected daily throughout the year. The study assessed the seasonality of provitamin A carotenoids in three plantain cultivars in response to climatic condition. Fruits were harvested at three maturities and freeze-dried before analysis. The results showed that there were high levels of the sun’s UV-B radiations throughout the year with the highest occurring from November to May when the area experienced clear skies with minimal cloud cover. These high levels of the sun’s UV-B index occurred between 9.00 h GMT and 17.00 h GMT. The study also showed that α-carotene content increased with maturity in “Apantu” during the rainy seasons ranging from 95 to 172 μg/100 g of dry pulp. Similar trends were observed during the dry season with a range of 28 to 489 μg/100 g. The α-carotene contents were very high in the periods of high sun’s UV-B radiations compared to the periods of low sun’s UV-B radiations. The α-carotene levels in the giant French plantains showed similar trends. Intermediate French “Oniaba” and False Horn “Apantu” plantain cultivar showed the highest content of β-carotene during the dry season. The high provitamin A carotenoid levels in the cultivars coincided with the high levels of the sun’s UV index.

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