Azide Conjugatable and pH Responsive Near-Infrared Fluorescent Imaging Probes

2009 ◽  
Vol 11 (23) ◽  
pp. 5386-5389 ◽  
Author(s):  
Julie Murtagh ◽  
Daniel O. Frimannsson ◽  
Donal F. O’Shea
Keyword(s):  
Near Infrared ◽  
Fluorescent Imaging ◽  
Ph Responsive ◽  
Imaging Probes

pH-Responsive near-infrared fluorescent cyanine dyes for molecular imaging based on pH sensing

2017 ◽  
Vol 53 (55) ◽  
pp. 7792-7795 ◽  
Author(s):  
Koji Miki ◽  
Kentaro Kojima ◽  
Kazuaki Oride ◽  
Hiroshi Harada ◽  
Akiyo Morinibu ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Near Infrared ◽  
Cyanine Dyes ◽  
Living Cells ◽  
Ph Sensing ◽  
Ph Responsive ◽  
Imaging Probes ◽  
Intracellular Compartments

pH-Responsive near-infrared cyanine dyes were synthesized and applied as imaging probes of acidic intracellular compartments of living cells.

Degradable pH-responsive NIR-II imaging probes based on a polymer-lanthanide composite for chemotherapy

2020 ◽  
Vol 49 (27) ◽  
pp. 9444-9453
Author(s):  
Miao Feng ◽  
Yanxing Wang ◽  
Bi Lin ◽  
Xiangrong Peng ◽  
Ying Yuan ◽  
...  
Keyword(s):  
Rare Earth ◽  
Near Infrared ◽  
Ph Sensitive ◽  
Ph Responsive ◽  
Imaging Probes ◽  
Rare Earth Nanoparticles

A pH-sensitive nanoprobe was proposed by combining hydrophobic rare earth nanoparticles with biocompatible nanomicelles for near infrared-II (NIR-II) imaging-guided chemotherapy.

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

Lysosomal targeted NIR photosensitizer for photodynamic therapy and two-photon fluorescent imaging

2021 ◽  
Author(s):  
Ruiyuan Liu ◽  
Yuping Zhou ◽  
Di Zhang ◽  
Genghan He ◽  
Chuang Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
High Fidelity ◽  
Design And Synthesis ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Design and synthesis of near-infrared (NIR) emissive fluorophore for imaging of organelle and photodynamic therapy has received enormous attention. Hence, NIR emissive fluorophore of high-fidelity lysosome targeting, two-photon fluorescence imaging,...

scholarly journals Bicyclic 1,3a,6a-Triazapentalene Chromophores: Synthesis, Spectroscopy and Their Use as Fluorescent Sensors and Probes

Chemosensors ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 16
Author(s):  
Yingchun Wang ◽  
Tomas Opsomer ◽  
Wim Dehaen
Keyword(s):  
Stokes Shift ◽  
Substituent Effects ◽  
Visible Spectrum ◽  
Fluorescent Sensors ◽  
Biological Imaging ◽  
Fluorescent Imaging ◽  
Fluorescence Properties ◽  
Imaging Probes ◽  
Synthetic Methodologies ◽  
Aromatic Heterocyclic

The 1,3a,6a-triazapentalene (TAP) is an aromatic heterocyclic fluorescent dye with interesting features such as its small size, large Stokes shift, solvatochromism, and emission wavelengths that are spread across the visible spectrum. TAPs have been synthesized via different synthetic strategies involving click−cyclization−aromatization domino reactions, gold-catalyzed cyclization of propargyl triazoles or triazolization of acetophenones. As a result, TAPs with diverse substitution patterns were obtained, showing varying fluorescence properties. Based on these properties, several TAPs have been selected and studied as fluorescent imaging probes in living cells and as sensors. This mini review provides an overview of the research on the bicyclic TAPs and does not comment on the literature about benzo or otherwise fused systems. The synthetic methodologies for the preparation of TAPs, the substituent effects on the fluorescence properties, and the behavior of the TAP core as an element of biological imaging probes and sensors are discussed.

scholarly journals Near Infrared Fluorescence Imaging of Intraperitoneal Ovarian Tumors in Mice Using Erythrocyte-Derived Optical Nanoparticles and Spatially-Modulated Illumination

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2544
Author(s):  
Joshua M. Burns ◽  
Elise Shafer ◽  
Raviraj Vankayala ◽  
Vikas Kundra ◽  
Bahman Anvari
Keyword(s):  
Fluorescence Imaging ◽  
Cytoreductive Surgery ◽  
Animal Studies ◽  
Near Infrared ◽  
Gynecological Cancer ◽  
Ovarian Tumors ◽  
Structured Illumination ◽  
Spatial Frequencies ◽  
Imaging Probes ◽  
Intraoperative Fluorescence Imaging

Ovarian cancer is the deadliest gynecological cancer. Cytoreductive surgery to remove primary and intraperitoneal tumor deposits remains as the standard therapeutic approach. However, lack of an intraoperative image-guided approach to enable the visualization of all tumors can result in incomplete cytoreduction and recurrence. We engineered nano-sized particles derived from erythrocytes that encapsulate the near infrared (NIR) fluorochrome, indocyanine green, as potential imaging probes for tumor visualization during cytoreductive surgery. Herein, we present the first demonstration of the use of these nanoparticles in conjunction with spatially-modulated illumination (SMI), at spatial frequencies in the range of 0–0.5 mm−1, to fluorescently image intraperitoneal ovarian tumors in mice. Results of our animal studies suggest that the nanoparticles accumulated at higher levels within tumors 24 h post-intraperitoneal injection as compared to various other organs. We demonstrate that, under the imaging specifications reported here, use of these nanoparticles in conjunction with SMI enhances the fluorescence image contrast between intraperitoneal tumors and liver, and between intraperitoneal tumors and spleen by nearly 2.1, and 3.0 times, respectively, at the spatial frequency of 0.2 mm−1 as compared to the contrast values at spatially-uniform (non-modulated) illumination. These results suggest that the combination of erythrocyte-derived NIR nanoparticles and structured illumination provides a promising approach for intraoperative fluorescence imaging of ovarian tumor nodules at enhanced contrast.

Photothermal liquid release from arrayed Au nanorod/hydrogel composites for chemical stimulation

2021 ◽  
Vol 32 (1) ◽  
pp. 015003
Author(s):  
Sang-Woo Seo ◽  
Youngsik Song ◽  
Hojjat Rostami Azmand
Keyword(s):  
Near Infrared ◽  
Local Heating ◽  
Temperature Response ◽  
Device Fabrication ◽  
Fluorescent Imaging ◽  
Silicon Membrane ◽  
Chemical Release ◽  
Nir Light ◽  
Fluorescein Dye ◽  
Hydrogel Composites

Abstract Controlled photothermal actuation of liquid release is presented using periodically arrayed hydrogel columns in a macroporous silicon membrane. Thermo-responsive hydrogel is mixed with Gold (Au) nanorods, and surface plasmon-induced local heating by near-infrared (NIR) light is utilized as an actuation method. We adopted theoretical modeling, which treats the hydrogel as a poro-viscoelastic medium to understand the mechanical and liquid transport properties of the hydrogel. To demonstrate the feasibility of the liquid release control using NIR light, we first characterized the temperature response of Au nanorod embedded hydrogel in the silicon membrane using its optical transmission behavior to confirm the successful device fabrication. Next, the liquid release characteristics from the structure were studied using fluorescent imaging of fluorescein dye solution while pulsed NIR light was illuminated on the structure. We successfully demonstrate that the liquid release can be controlled using remote NIR illumination from the presented structure. Considering the periodically arrayed configuration with high spatial resolution, this will have a potential prospect for optically-addressable chemical release systems, which benefit retina prosthesis interfaces.

A Reduction Active Theranostic Nanoparticle for Enhanced Near-Infrared Imaging and Phototherapy by Reducing Glutathione Level in Cancer Cells

2021 ◽  
Vol 21 (12) ◽  
pp. 5965-5971
Author(s):  
Xiaofang Song ◽  
Lifo Ruan ◽  
Tianyu Zheng ◽  
Jun Wei ◽  
Jiayu Zhang ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Disulfide Bonds ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Fluorescent Imaging ◽  
Self Monitoring ◽  
Fluorescent Intensity ◽  
Turn On ◽  
Hyperbranched Poly ◽  
One Step

Facile preparation of a tumoral-stimuli-activated theranostic nanoparticle with simple constituents remains a challenge for tumor theranostic nanosystems. Herein we design a simple reductionresponsive turn-on theranostic nanoparticle for achieving fluorescent imaging and phototherapy combination. The theranostic nanoparticle is prepared by a simple one-step dialysis method of reduction active amphiphilic hyperbranched poly(β-amidoamines) and a near-infrared (NIR) dye indocyanine green (ICG). The fluorescence of ICG is quenched by the aggregation-caused quenching (ACQ) effect. The fluorescent intensity of free ICG at 816 nm was ∼40 times as high as that of particulate ICG. After reductive nanoparticles incubated with dithiothreitol (DTT), the size of the nanoparticles increased from 160 nm to 610 nm by Dynamic light scattering (DLS). As nanoparticles were internalized by cancer cells, the disulfide bonds would be cleaved by intracellular reduction agents like glutathione (GSH), leading to the release of entrapped ICG. The released ICG regained its fluorescence for self-monitoring the release and therapeutic effect of ICG by fluorescence spectra and the quantitative evaluation of NIR fluorescence intensity. Remarkably, nanoparticles can also reinforce antitumor efficacy through photodynamic therapy and GSH depletion property. This study provides new insights into designing turn-on theranostic systems.

scholarly journals Photothermal-assisted antibacterial application of GO-Ag against clinical isolated MDR E. coli

2019 ◽  
Author(s):  
Yuqing Chen ◽  
Wei Wu ◽  
Zeqiao Xu ◽  
Cheng Jiang ◽  
Shuang Han ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Near Infrared ◽  
Antibacterial Effect ◽  
Luria Bertani ◽  
Fluorescent Imaging ◽  
Photothermal Effect ◽  
Antibacterial Efficiency ◽  
E Coli ◽  
Morphology Characterization

Abstract Background: Treatment of multidrug-resistant (MDR) bacterial infection is a great challenge in public health. Herein, we provide a solution to this problem with the use of graphene oxide-silver (GO-Ag) nanocomposites as anti-bacterial agent. Methods: Following established protocols, silver nanoparticles were grown on graphene oxide sheets. Then, a series of in-vitro studies were conducted to validate the antibacterial efficiency of the GO-Ag nanocomposites against clinical MDR Escherichia coli (E. coli) strains. Firstly, minimum inhibitory concentrations (MICs) of different antimicrobials were tested against MDR E. Coli strains. Then, bacteria viability assessments were conducted with different nanomaterials in Luria-Bertani (LB) broth. Afterwards, photothermal irradiation was conducted on MDR E. coli with lower GO-Ag concentration. At last, fluorescent imaging and morphology characterization using scanning electron microscope (SEM) were done to find the possible cause of antibacterial effect. Results: GO-Ag nanocomposites showed the highest antibacterial efficiency among tested antimicrobials. Synergetic antibacterial effect was observed in GO-Ag nanocomposites treated group. The remained bacteria viabilities were 4.4% and 4.1% respectively for different bacteria strains with GO-Ag concentration at 14.0 µg mL-1. In addition, GO-Ag nanocomposites have strong absorption in the near-infrared field and can convert the electromagnetic energy to heat. With the use of this photothermal effect, effective sterilization could be achieved using GO-Ag nanocomposites concentration as low as 7.0 µg mL-1. Fluorescent imaging and morphology characterization were used to analyze bacteria living status, which uncovered that bacteria integrity was disrupted after GO-Ag nanocomposites treatment. Conclusions: GO-Ag nanocomposites are proved to be efficient antibacterial agent against multi-drug resistant E. coli. Their strong antibacterial effect arises from inherent antibacterial property and photothermal effect that provides aid for bacteria killing.

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