A Reduction Active Theranostic Nanoparticle for Enhanced Near-Infrared Imaging and Phototherapy by Reducing Glutathione Level in Cancer Cells

2021 ◽  
Vol 21 (12) ◽  
pp. 5965-5971
Author(s):  
Xiaofang Song ◽  
Lifo Ruan ◽  
Tianyu Zheng ◽  
Jun Wei ◽  
Jiayu Zhang ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Disulfide Bonds ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Fluorescent Imaging ◽  
Self Monitoring ◽  
Fluorescent Intensity ◽  
Turn On ◽  
Hyperbranched Poly ◽  
One Step

Facile preparation of a tumoral-stimuli-activated theranostic nanoparticle with simple constituents remains a challenge for tumor theranostic nanosystems. Herein we design a simple reductionresponsive turn-on theranostic nanoparticle for achieving fluorescent imaging and phototherapy combination. The theranostic nanoparticle is prepared by a simple one-step dialysis method of reduction active amphiphilic hyperbranched poly(β-amidoamines) and a near-infrared (NIR) dye indocyanine green (ICG). The fluorescence of ICG is quenched by the aggregation-caused quenching (ACQ) effect. The fluorescent intensity of free ICG at 816 nm was ∼40 times as high as that of particulate ICG. After reductive nanoparticles incubated with dithiothreitol (DTT), the size of the nanoparticles increased from 160 nm to 610 nm by Dynamic light scattering (DLS). As nanoparticles were internalized by cancer cells, the disulfide bonds would be cleaved by intracellular reduction agents like glutathione (GSH), leading to the release of entrapped ICG. The released ICG regained its fluorescence for self-monitoring the release and therapeutic effect of ICG by fluorescence spectra and the quantitative evaluation of NIR fluorescence intensity. Remarkably, nanoparticles can also reinforce antitumor efficacy through photodynamic therapy and GSH depletion property. This study provides new insights into designing turn-on theranostic systems.

scholarly journals Designing the nanobiointerface of fluorescent nanodiamonds: highly selective targeting of glioma cancer cells

Nanoscale ◽  
2015 ◽  
Vol 7 (2) ◽  
pp. 415-420 ◽  
Author(s):  
Jitka Slegerova ◽  
Miroslav Hajek ◽  
Ivan Rehor ◽  
Frantisek Sedlak ◽  
Jan Stursa ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Polymer Coating ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Glioma Cells ◽  
Near Infrared Imaging ◽  
Rgd Peptides ◽  
Selective Targeting ◽  
Highly Effective ◽  
Fluorescent Nanodiamonds

Highly effective targeting and background-free, near-infrared imaging of glioma cells was achieved using new nanoparticles based on fluorescent nanodiamonds. The used polymer coating with attached cyclic RGD peptides ensured unusually selective targeting.

A near-infrared light-controlled, ultrasensitive one-step photoelectrochemical detection of dual cell apoptosis indicators in living cancer cells

2020 ◽  
Vol 56 (60) ◽  
pp. 8488-8491
Author(s):  
Ying-Ning Zheng ◽  
Cheng-Yi Xiong ◽  
Ying Zhuo ◽  
Ya-Qin Chai ◽  
Wen-Bin Liang ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cell Apoptosis ◽  
Adenosine Triphosphatase ◽  
Near Infrared ◽  
Infrared Light ◽  
Sodium Potassium ◽  
Near Infrared Light ◽  
Nir Light ◽  
One Step

The proposed near-infrared (NIR) light-controlled, one-step photoelectrochemical (PEC) strategy could simultaneously detect cell apoptosis indicators, phosphatidylserine (Pho) and sodium potassium adenosine triphosphatase (Sat), on living cancer cells.

scholarly journals Methylene Blue—Current Knowledge, Fluorescent Properties, and Its Future Use

2020 ◽  
Vol 9 (11) ◽  
pp. 3538
Author(s):  
Tomasz Cwalinski ◽  
Wojciech Polom ◽  
Luigi Marano ◽  
Giandomenico Roviello ◽  
Alberto D’Angelo ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Methylene Blue ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Current Knowledge ◽  
Early Stage ◽  
Surgical Techniques ◽  
Fluorescent Imaging ◽  
Pancreatic Tumors ◽  
Fluorescent Properties

Methylene blue is a fluorescent dye discovered in 1876 and has since been used in different scientific fields. Only recently has methylene blue been used for intraoperative fluorescent imaging. Here, the authors review the emerging role of methylene blue, not only as a dye used in clinical practice, but also as a fluorophore in a surgical setting. We discuss the promising potential of methylene blue together with the challenges and limitations among specific surgical techniques. A literature review of PubMed and Medline was conducted based on the historical, current and future usage of methylene blue within the field of medicine. We reviewed not only the current usage of methylene blue, but we also tried to grasp its’ function as a fluorophore in five main domains. These domains include the near-infrared imaging visualization of ureters, parathyroid gland identification, pancreatic tumors imaging, detection of breast cancer tumor margins, as well as breast cancer sentinel node biopsy. Methylene blue is used in countless clinical procedures with a relatively low risk for patients. Usage of its fluorescent properties is still at an early stage and more pre-clinical, as well as clinical research, must be performed to fully understand its potentials and limitations.

Preparation of Composite Cypate Nanoparticles and Its Application in the Treatment of Pediatric Bladder Tumors

2021 ◽  
Vol 21 (2) ◽  
pp. 868-877
Author(s):  
Ling Wang ◽  
Hong Tao ◽  
Zhigang Li ◽  
Yi Lei ◽  
Bing Bai ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Negative Impact ◽  
Particle Diameter ◽  
Photothermal Effect ◽  
Average Particle ◽  
Bladder Cancer Cells

Cypate is an organic material with tumor treatment function, which has photothermal effect. Based on the characteristics of this material, this study adopted the coupling method to obtain FB-Cypate nano-microspheres, BDI-1-FB-Cypate albumin nano-microsphere diad and CPPs-BDI-1-FB-Cypate albumin nano-microsphere triad. Cypate and FB-Cypate materials were characterized by scanning TEM, particle size analyzer, and spectrophotometer. An injection-ureter introduction device was used to perfuse pediatric bladder cancer cells into the bladder cavity of nude mice, and different Cypate-type material solutions were perfused into the bladder cavity at the same time. Then, the anti-bladdertumor performance of different materials on tumor cells was compared. The test proves that the average particle diameter of FB-Cypate material is (102.3 ±8.6) nm, which can be used for near-infrared imaging, and the temperature rise is obvious under light conditions. In the test of inhibiting bladder cancer cells in children, the use of Cypate type materials can significantly inhibit the survival of bladder tumor cells, and it has less negative impact on physiological functions after surgery.

Targeted Near-Infrared Fluorescent Turn-on Nanoprobe for Activatable Imaging and Effective Phototherapy of Cancer Cells

2015 ◽  
Vol 8 (24) ◽  
pp. 15013-15023 ◽  
Author(s):  
Na Li ◽  
Tingting Li ◽  
Chao Hu ◽  
Xiaomin Lei ◽  
Yunpeng Zuo ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Near Infrared ◽  
Turn On

Bioinformatic inspired nanoparticles engineered for enhanced delivery to the bone.

2019 ◽  
Vol 37 (15_suppl) ◽  
pp. e14637-e14637
Author(s):  
Andrew Gdowski ◽  
Amalendu Ranjan ◽  
Jana Lampe ◽  
Victor Lin ◽  
Yu-Chieh Wang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Polymeric Nanoparticles ◽  
Extrusion Process ◽  
In Vitro Testing ◽  
Physico Chemical

e14637 Background: We have engineered a programmable-bioinspired nanoparticle (P-BiNP) delivery system to simultaneously target the bone and increase uptake in homotypic tumor cells by coating polymeric nanoparticles with programmed cancer cell membranes. This approach is unique in that we have incorporated relevant clinical bioinformatics data to guide the design of these nanoparticles. Methods: A gene ontology search identified potential homotypic cell-cell adhesion genes. These were cross referenced with RNAseq expression levels from patients with metastatic prostate cancer to various organ locations using the Cbioportal database. C4-2B prostate cancer cells were grown and stimulated to express membrane ɑVβ3 by treatment with recombinant human CXCL12. The cell membrane from these cancer cells were used to coat polymeric nanoparticles by an extrusion process. Physico-chemical optimization and characterization of the P-BiNPs was performed. In vitro testing was done with flow cytometry and MTT assays. A murine model was used with high resolution near infrared imaging to identify nanoparticle localization. Results: Analysis of RNAseq from mCRPC samples (n = 118) identified ITGB3 as increased in bone metastatic lesions compared to metastases from other sites (P < 0.0001). ITGB3 is a subunit of integrin ɑVβ3 and was selected as a target for enhancement to improve P-BiNP homotypic targeting and bone localization. After optimization, P-BiNPs showed optimal physico-chemical characteristics for drug delivery. Both in vitro testing and in vivo testing showed the P-BiNPs to be superior in homotypic uptake and localization to the bone. Conclusions: This platform of identifying clinically relevant targets for dual homotypic and bone targeting has potential as a strategy for treatment and imaging modalities in cancers that affects the bone as well as implications for delivering nanoparticles to other organs of interest.

scholarly journals Comprehensive Effects of Near-Infrared Multifunctional Liposomes on Cancer Cells

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1098 ◽  
Author(s):  
Yiqing Deng ◽  
Huaying Huang ◽  
Mengxiao Chen ◽  
Gang Chen ◽  
Wangcai Zou ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Cancer Cells ◽  
High Frequency ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Alternating Magnetic Field ◽  
Near Infrared Imaging ◽  
Control Drug ◽  
Anti Cancer ◽  
Thermosensitive Liposomes

Multifunctional theranostic systems are a recent important development of medical research. We combined the characteristics of near-infrared luminescent quantum dots and thermosensitive magnetoliposomes to develop a multifunctional nano-diagnostic material. This system is based on near-infrared magnetic thermosensitive liposomes, which encapsulate drugs and can control drug localization and release. After incubating cancer cells with the liposomes, the state of the cells was analyzed in real time by near-infrared imaging. Cell viability was significantly inhibited by heat treatment or alternating magnetic field treatment, which thus improved the anti-cancer properties of the liposomes. In the future, by combining near-infrared imaging technology and an external high-frequency alternating magnetic field, we could not only detect cancer cells noninvasively but also conduct image-guided treatments for cancer.

Targeted biological effect of an affitoxin composed of an HPV16E7 affibody fused with granzyme B (ZHPV16E7-GrB) against cervical cancer in vitro and in vivo

2020 ◽  
Vol 20 ◽  
Author(s):  
Wenhuan Wang ◽  
Xiaochun Tan ◽  
Jie Jiang ◽  
Yiqi Cai ◽  
Fangfang Feng ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Fusion Protein ◽  
Cancer Cells ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Granzyme B ◽  
Tumour Bearing ◽  
Cervical Cancer Cells

Background: High risk type 16 of human papillomavirus (HPV16) is associated with 50% of cervical cancer, for which reliable targeted therapies are lacking. HPV early protein 7 (E7) is an oncoprotein responsible for cell malignant transformation. In our previous work, a highly specific affibody targeting HPV16E7 (ZHPV16E7) was developed. Objective: In order to improve the targeted therapeutic effect, the present study prepared an affitoxin consisting of ZHPV16E7 fused with granzyme B (GrB), namely, ZHPV16E7-GrB, and evaluated its targeting action in vitro and in vivo. Methods: The ZHPV16E7-GrB fusion protein was produced in a prokaryotic expression system. The targeted binding properties of the ZHPV16E7-GrB to the HPV16E7 were confirmed by immunofluorescence assay (IFA) in cervical cancer cell lines, by immunohistochemical assay (IHA) in cervical cancer tissue from clinical specimens and by near-infrared imaging in tumour-bearing mice. The anti-tumour effect on both cervical cancer cells in vitro and tumour-bearing mice in vivo were further evaluated. Results: A 34-kDa ZHPV16E7-GrB fusion protein was produced in E. coli and displayed corresponding immunoreactivity. IFA revealed that ZHPV16E7-GrB bound specifically to HPV16-positive TC-1 and SiHa cells. IHA showed that ZHPV16E7-GrB also bound specifically to HPV16-positive clinical tissue specimens. In addition, the near-infrared imaging results showed that ZHPV16E7-GrB was enriched in tumour tissues. Moreover, both the ZHPV16E7-GrB affitoxin and ZHPV16E7 affibody (without GrB) significantly reduced the proliferation of cervical cancer cells in vitro and tumour-bearing mice in vivo, and the antiproliferative effect of ZHPV16E7-GrB was higher than that of the ZHPV16E7 affibody. Conclusions: The affitoxin by coupling the affibody with GrB is a promising targeted therapeutic agent with the dual advantages of the targeted affibody and the GrB cytotoxin.

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