Genetic Studies of the Relationship of Tumour–Host Cells: Effect of Tumour Cells killed by X-rays upon the Growth of Admixed Viable Cells

Pannexins (Panx) are proteins that form functional single membrane channels, but they have not yet been described in dogs. The aim of the present study was to detect Panx1, Panx2 and Panx3 in frozen–thawed dog spermatozoa using flow cytometry and immunofluorescence analyses, evaluating the relationship of these proteins with propidium iodide (PI) in frozen–thawed spermatozoa. Fresh and frozen–thawed dog spermatozoa from eight dogs were preincubated with 3 μM PI with or without 15 μM carbenoxolone (CBX) or 1 mM probenecid (PBD), two Panx channel inhibitors, and then incubated with rabbit anti-Panx1, anti-Panx2 and anti-Panx3 antibodies (1 : 200). Panx immunolocalisation was assessed by fluorescence microscopy. Flow cytometry data were evaluated by analysis of variance. All three Panx proteins were found in dog spermatozoa: Panx1 was mostly localised to the acrosomal and equatorial segment, Panx2 was found in the posterior region of the head and tail and Panx3 was localised to the equatorial and posterior head segment. The percentage of PI-positive cells determined by flow cytometry was reduced (P < 0.05) in the presence of Panx inhibitors. These results show that Panx proteins are present in dog spermatozoa and increase PI permeability in frozen–thawed dog sperm, suggesting that the percentage of PI-positive spermatozoa used as an indicator of non-viable cells may lead to overestimation of non-viable cells.

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Difficulties with Positioning Robertshaw Double Lumen Tubes

Anaesthesia and Intensive Care ◽

10.1177/0310057x7500300404 ◽

1975 ◽

Vol 3 (4) ◽

pp. 299-311 ◽

Cited By ~ 15

Author(s):

A. M. S. Black ◽

G. A. Harrison

Keyword(s):

Tracheobronchial Tree ◽

Precise Definition ◽

X Rays ◽

Double Lumen ◽

Definition Of ◽

Relationship Of ◽

The Relationship

Difficulties in positioning Robertshaw tubes have been reviewed in a retrospective and a prospective series. Pre-operative chest X-rays did not help in predicting difficulties. The performance of the Robertshaw tube was carefully recorded for each malposition in the prospective series, and the relationship of the malplaced tube to the tracheobronchial tree was reconstructed. This exercise allowed a more precise definition of the dangers of malpositions and formulation of procedure to minimize their incidence.

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Family and genetic studies on the relationship of schizophrenia to affective disorders

European Archives of Psychiatry and Clinical Neuroscience ◽

10.1007/pl00014186 ◽

1999 ◽

Vol 249 (S4) ◽

pp. S57-S61 ◽

Cited By ~ 29

Author(s):

W. Maier ◽

Marcella Rietschel ◽

Dirk Lichtermann ◽

Dieter B. Wildenauer

Keyword(s):

Affective Disorders ◽

Genetic Studies ◽

Relationship Of ◽

The Relationship

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Mammographic density as marker of increased risk of breast cancer

Medical alphabet ◽

10.33667/2078-5631-2021-19-41-48 ◽

2021 ◽

pp. 41-48

Author(s):

P. G. Labazanova ◽

M. V. Budanova ◽

I. I. Burdina ◽

S. B. Zapirova ◽

M. L. Mazo ◽

...

Keyword(s):

Breast Cancer ◽

Mammographic Density ◽

Molecular Genetic ◽

Breast Cancer Risk Factor ◽

Develop Breast Cancer ◽

Reproductive Age ◽

Genetic Studies ◽

Increased Risk ◽

Relationship Of ◽

The Relationship

‘Mammographic density’ (MD) is a concept that has entered medical practice since 2017. as a marker of breast cancer risk factor (BC) according to the international classifiation of NCCN. The term reflcts the degree of severity of benign diffuse breast dysplasia in women of post-reproductive age. MD is determined by the ratio of stromal, epithelial, and adipose tissue. According to the literature, in young women, high MD limits the possibilities of X-ray mammography, reducing its effectiveness in oncomammoscreening, leading to the detection of advanced forms of breast cancer. Post-reproductive women with high MD are more likely to develop breast cancer than those with low MD. In this regard, MD is of particular interest for studying its role in oncogenesis. Recent molecular genetic studies of the differences between high and low MD explain the main biological reasons why post-reproductive women with dense breast structure are at a higher risk of developing breast cancer. The aim is to identify the factors that inflence the relationship of MD with the risk of developing breast cancer based on a comparative analysis of molecular genetic studies and radiological manifestations of MD of different severity and to identify the factors that contribute to the formation of MD variants.

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Hsa-miR-196a2 Rs 11614913 C>T polymorphism and CRC susceptibility: A case-control study.

Journal of Clinical Oncology ◽

10.1200/jco.2020.38.15_suppl.e16066 ◽

2020 ◽

Vol 38 (15_suppl) ◽

pp. e16066-e16066

Author(s):

Li-zhu Chen ◽

Yu Chen ◽

Wei-feng Tang ◽

Lin Chen ◽

Jin Lin ◽

...

Keyword(s):

Alcohol Use ◽

Smoking Status ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Genetic Studies ◽

Incidence And Mortality ◽

Genes Encoding ◽

Pcr Method ◽

Relationship Of ◽

The Relationship

e16066 Background: The incidence and mortality rate of Colorectal cancer (CRC) is high worldwide. Many genetic studies have suggested that single nucleotide polymorphisms (SNPS) in genes encoding small molecule RNAs were associated with CRC risk, but the results were different in different studies. In our study, we investigated the general demographic characteristics and the relationship of hsa-mir-196a2 rs11614913 C > T polymorphism and CRC susceptibility in Chinese CRC. Methods: Our study included 1,003 CRC patients and 1,303 controls. Restriction fragment length polymorphism (RFLP-PCR) method was used for genotyping and SAS version 9.4 software for statistical analysis. Results: The incidence of smoking status,alcohol use and BMI in the CRC group (25.82%,17.35%,66.80%) were much higher than that in the control subjects (20.34%,10.44%,52.80%) respectively(P < 0.05). After adjusting age and other factors, hsa-mir-196a2 rs11614913 C > T genotypes were not statistically correlated with CRC risk and tumor location.But the TT genotype in the hsa-mir-196a2 rs11614913 C > T polymorphism reduced the risk of CRC in women (OR = 0.64, 95 CI: 0.42-0.97, P = 0.036). Conclusions: Smoking status,alcohol use and BMI may be main risk factors for CRC development in our study population.The polymorphism of hsa-mir-196a2 rs11614913 C > T gene may affect the risk of CRC in women, which requires further investigation in a larger cohort in the future.

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The influence of the host plant in inducing parasitism in lucerne and clover nodules

Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character ◽

10.1098/rspb.1930.0015 ◽

1930 ◽

Vol 106 (742) ◽

pp. 110-122 ◽

Cited By ~ 35

Keyword(s):

Host Plant ◽

Vascular Supply ◽

Host Cells ◽

Nodule Bacteria ◽

Normal Functioning ◽

Single Strain ◽

Energy Material ◽

Relationship Of ◽

The Relationship ◽

Inoculation Group

The relationship of the nodule organism to its host plant has been much discussed, some authors regarding it as an instance of true symbiosis, while others regard the organism as a parasite to which the host plant offers a certain resistance. Failures to obtain inoculation of legumes with strains of nodule bacteria belonging to a different inoculation group may be regarded as examples of such resistance. Even where nodules are formed, the fixation of nitrogen and the benefit derived by the host plant varies according to the strain of the nodule organism concerned (Stevens (1) and Wright (2) ). Some strains, while producing nodules, cause no increase in growth or nitrogen content in the host plant (3) and (4). It is uncertain whether the resistance of the host plant prevents the normal functioning of such strains, or whether they are actively parasitic on the nodule tissue. Strains of the nodule organism thus differ in their relationship to the host plant. The behaviour of a single strain in the tissues may also be altered by the condition of the host plant. Thus, when Vicia faba is grown in a boron-deficient solution, the conducting tissue develops abnormally, so that the vascular supply to the nodules is either absent or incomplete. In such nodules the bacteria fix but little nitrogen and destroy the host cells in which they lie, although the same strain in healthy plants behaves normally and fixes appreciable amounts of nitrogen (Brenchley and Thornton (5) ). It is thus possible experimentally to alter the relationship between the host plant and the bacteria, so that a strain of the latter which is normally beneficial to its host becomes actively parasitic. It was suggested that, in normal nodules, the bacteria derive their energy material from the carbohydrates conveyed to them along the vessels, but that in boron-deficient plants they are to a large extent deprived of their carbohydrates, owing to failure of the vascular supply, and derive energy by attacking the host protoplasm. If this hypothesis be correct, it should be possible to induce the change from symbiosis to parasitism by cutting off the carbohydrate supply in other ways, for example by keeping the plants in darkness.

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THE MAXIMUM ENERGY OF ħω INDUCED BY N+, N2+ AND N3+ IONS

International Journal of PIXE ◽

10.1142/s0129083596000077 ◽

1996 ◽

Vol 06 (01n02) ◽

pp. 59-63

Author(s):

C.C. Hsu ◽

T.C. Chu ◽

Y.C. Chang ◽

H.H. Hsu

Keyword(s):

Charge State ◽

Atomic Number ◽

Maximum Energy ◽

Target Atom ◽

X Rays ◽

X Ray ◽

Relationship Of ◽

The Relationship

The continuous X-ray of Be, Ni, and Cd targets induced by N +, N 2+, N 3+ ions were measured at the projectile energies of 50-, 70-, 100-, 150-, and 200-keV. Since the energy of the projectile is so low and the detecting limit of the detector is about one keV, the continuous X-ray would be the X-rays of nuclear and atomic bremsstrahlung (NB, AB). From the result of maximum energy of ħω. the relationship of a maximum energy ħω of below: [Formula: see text] Which indicates that the maximum energy of ħω depends on (-3/5) power of the atomic number of projectile, Zp. and on (-1/3q) power of the atomic number of target atom, Zt, and on the energy of projectile. Where q expresses the number of charge state of the projectile.

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On the Thermal Interpretation of Hard X-Ray Bursts from Solar Flares

Symposium - International Astronomical Union ◽

10.1017/s0074180900234554 ◽

1974 ◽

Vol 57 ◽

pp. 395-412 ◽

Cited By ~ 1

Author(s):

John C. Brown

Keyword(s):

Solar Flares ◽

Thermal Conduction ◽

Temperature Structure ◽

X Rays ◽

X Ray ◽

Time Variations ◽

Relationship Of ◽

First Time ◽

The Relationship ◽

Continuous Temperature

The possible validity of thermal bremsstrahlung models of flare hard X-ray bursts is investigated quantitatively. In particular, the problem of rapid thermal conduction in ‘multi-temperature’ models is adequately examined for the first time by using a continuous temperature distribution consistent with the observed X-ray spectrum. This distribution is obtained from a general analytic solution for the temperature structure required to mimick any ‘non-thermal’ spectrum, the method being equally applicable to cosmic sources.It is concluded that the thermal interpretation might extend to X-rays of hundreds of keV, a result with important consequences for flare energetics. The relationship of such a model to observations of X-ray polarization and rapid time variations is also considered.

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