scholarly journals Training associated alterations in equine respiratory immunity using a multiomics comparative approach

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Anna E. Karagianni ◽  
Dominic Kurian ◽  
Eugenio Cillán-Garcia ◽  
Samantha L. Eaton ◽  
Thomas M. Wishart ◽  
...  
Keyword(s):  
Airway Inflammation ◽  
Methodological Approach ◽  
Strong Association ◽  
Comparative Approach ◽  
Biological Processes ◽  
Period Gene ◽  
Thoroughbred Racehorses ◽  
Gene And Protein Expression ◽  
Pulmonary Immune System ◽  
Insight Into

AbstractNeutrophilic airway inflammation is highly prevalent in racehorses in training, with the term mild to moderate equine asthma (MMEA) being applied to the majority of such cases. Our proposed study is largely derived from the strong association between MMEA in racehorses and their entry into a race training program. The objectives of this study are to characterise the effect of training on the local pulmonary immune system by defining the gene and protein expression of tracheal wash (TW) derived samples from Thoroughbred racehorses prior to and following commencement of race training. Multiomics analysis detected 2138 differentially expressed genes and 260 proteins during the training period. Gene and protein sets were enriched for biological processes related to acute phase response, oxidative stress, haemopoietic processes, as well as to immune response and inflammation. This study demonstrated TW samples to represent a rich source of airway cells, protein and RNA to study airway immunity in the horse and highlighted the benefits of a multiomics methodological approach to studying the dynamics of equine airway immunity. Findings likely reflect the known associations between race-training and both airway inflammation and bleeding, offering further insight into the potential mechanisms which underpin training associated airway inflammation.

scholarly journals Training Associated Alterations in Equine Respiratory Immunity Using a Multiomics Comparative Approach

2021 ◽  
Author(s):  
Anna Eleonora Karagianni ◽  
Dominic Kurian ◽  
Eugenio Cillán-Garcia ◽  
Samantha L. Eaton ◽  
Thomas M. Wishart ◽  
...  
Keyword(s):  
Airway Inflammation ◽  
Methodological Approach ◽  
Strong Association ◽  
Comparative Approach ◽  
Biological Processes ◽  
Period Gene ◽  
Thoroughbred Racehorses ◽  
Gene And Protein Expression ◽  
Pulmonary Immune System ◽  
Insight Into

Abstract Neutrophilic airway inflammation is highly prevalent in racehorses in training, with the term mild to moderate equine asthma (MMEA) being applied to the majority of such cases. Our proposed study is largely derived from the strong association between MMEA in racehorses and their entry into a race training program. The objectives of this study are to characterise the effect of training on the local pulmonary immune system by defining the gene and protein expression of tracheal wash (TW) derived samples from Thoroughbred racehorses prior to and following commencement of race training. Multiomics analysis detected 2,138 differentially expressed genes and 260 proteins during the training period. Gene and protein sets were enriched for biological processes related to acute phase response, oxidative stress, haemopoietic processes, as well as to immune response and inflammation. This study demonstrated TW samples to represent a rich source of airway cells, protein and RNA to study airway immunity in the horse and highlighted the benefits of a multiomics methodological approach to studying the dynamics of equine airway immunity. Findings likely reflect the known associations between race-training and both airway inflammation and bleeding, offering further insight into the potential mechanisms which underpin training associated airway inflammation.

scholarly journals Training-Associated Alterations In Equine Respiratory Immunity – A Multi-Omics Comparative Approach.

2021 ◽  
Author(s):  
Anna Eleonora Karagianni ◽  
Dominic Kurian ◽  
Eugenio Cillán-Garcia ◽  
Samantha Eaton ◽  
Thomas M. Wishart ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Expression ◽  
Airway Inflammation ◽  
Cell Function ◽  
Immune Cell ◽  
Methodological Approach ◽  
Training Period ◽  
Comparative Approach ◽  
Thoroughbred Racehorses ◽  
Gene And Protein Expression

Abstract Background: Neutrophilic airway inflammation is highly prevalent in racehorses in training, with the term mild to moderate equine asthma (MMEA) being applied to the majority of such cases. Our proposed study is largely derived from the strong association between MMEA in racehorses and their entry into a race training program; this has led to our primary aim of measuring the effect of race training on pulmonary immune cell function. The objectives of this study are to characterise the effect of training on the local pulmonary immune system and quantify the magnitude of effect by defining (a) the gene expression of tracheal wash (TW) derived cells and (b) the protein expression of TW samples derived from Thoroughbred racehorses prior to (T0) and following commencement of race training (T1). Results: Tracheal wash samples were obtained at both time points (T0, T1) from the same Thoroughbred horses (n=16). Gene expression of TW derived cells, determined by RNAseq and analysed by DEseq2 detected 2,138 differentially expressed (DE) genes during the training period; 1,122 of these were upregulated. The proteome of TW samples was also evaluated and contained 260 DE proteins during the training period; 103 of these were upregulated and the rest downregulated. Gene and protein sets were enriched for biological processes related to acute phase response and oxidative stress, as well as to immune response and inflammation. Many genes, including ISG15, ISG20, IFI35, SOCS1 and TRIM21, were highly enriched for IFN signaling. Interestingly, pathway analysis also highlighted genes and proteins related to haemopoietic processes.Conclusions: This study demonstrated TW samples to represent a rich source of airway cells, protein and RNA to study airway immunity in the horse and highlighted the benefits of a multi-omics methodological approach to studying the dynamics of equine airway immunity. Intense training induced quantifiable alterations in both gene and protein expression of airway derived cells is consistent with deregulation of airway immunity and haemopoietic abnormalities. Respectively, these findings likely reflect the known associations between race training and both airway inflammation and bleeding, in particular offering further insight into the potential mechanisms which underpin training associated airway inflammation.

An Insight into Oligopeptide Transporter 3 (OPT3) family proteins

2020 ◽  
Vol 27 ◽  
Author(s):  
Fırat Kurt
Keyword(s):  
Stress Responses ◽  
Chelating Agent ◽  
Biological Processes ◽  
Biotic And Abiotic Stress ◽  
Oligopeptide Transporter ◽  
Binding Residues ◽  
Fe Homeostasis ◽  
Proteins Interactions ◽  
Insight Into ◽  
Selection Of

: Oligopeptide transporter 3 (OPT3) proteins are one of the subsets of OPT clade, yet little is known about these transporters. Therefore, homolog OPT3 proteins in several plant species were investigated and characterized using bioinformatical tools. Motif and co-expression analyses showed that OPT3 proteins may be involved in both biotic and abiotic stress responses as well as growth and developmental processes. AtOPT3 usually seemed to take part in Fe homeostasis whereas ZmOPT3 putatively interacted with proteins involved in various biological processes from plant defense system to stress responses. Glutathione (GSH), as a putative alternative chelating agent, was used in the AtOPT3 and ZmOPT3 docking analyses to identify their putative binding residues. The information given in this study will contribute to the understanding of OPT3 proteins’ interactions in various pathways and to the selection of potential ligands for OPT3s.

scholarly journals Detecting Convergence Trends among EU Universities

Economies ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 42
Author(s):  
Maria Adamakou ◽  
Dimitris Kallioras ◽  
George Petrakos
Keyword(s):  
Methodological Approach ◽  
Basic Research ◽  
Valuable Insight ◽  
Traditional Role ◽  
University Ranking ◽  
Convergence Clubs ◽  
Development Processes ◽  
The Impact ◽  
The Empirical Analysis ◽  
Insight Into

Universities are emerging growth determinants. This is so as, in addition to the fulfillment of their traditional role in teaching and (basic) research, universities, as aptly described within the helix framework, are expected to engage in regional development processes. The paper aims to detect trends of convergence among EU universities in terms of performance. To the best of our knowledge, this topic has not hitherto been examined. The empirical analysis of the paper covers the period 2014–2021, utilizes data obtained from URAP (University Ranking by Academic Performance), and employs the methodological approach of convergence clubs. The findings of the paper provide valuable insight into both theory and policy-making. We conclude that despite the unification of EU educational space, trends of divergence among EU universities are still present, and notable divisions still remain. Consequently, this indicates that the impact of EU universities on the formation of spatial disparities across EU space is not neutral.

scholarly journals Geo-Spatial Analysis of Population Density and Annual Income to Identify Large-Scale Socio-Demographic Disparities

2021 ◽  
Vol 10 (7) ◽  
pp. 432
Author(s):  
Nicolai Moos ◽  
Carsten Juergens ◽  
Andreas P. Redecker
Keyword(s):  
Population Density ◽  
Large Scale ◽  
Methodological Approach ◽  
Spatial Relationship ◽  
Postal Code ◽  
Annual Income ◽  
Choropleth Maps ◽  
Clustering Approach ◽  
Relationship Of ◽  
Insight Into

This paper describes a methodological approach that is able to analyse socio-demographic and -economic data in large-scale spatial detail. Based on the two variables, population density and annual income, one investigates the spatial relationship of these variables to identify locations of imbalance or disparities assisted by bivariate choropleth maps. The aim is to gain a deeper insight into spatial components of socioeconomic nexuses, such as the relationships between the two variables, especially for high-resolution spatial units. The used methodology is able to assist political decision-making, target group advertising in the field of geo-marketing and for the site searches of new shop locations, as well as further socioeconomic research and urban planning. The developed methodology was tested in a national case study in Germany and is easily transferrable to other countries with comparable datasets. The analysis was carried out utilising data about population density and average annual income linked to spatially referenced polygons of postal codes. These were disaggregated initially via a readapted three-class dasymetric mapping approach and allocated to large-scale city block polygons. Univariate and bivariate choropleth maps generated from the resulting datasets were then used to identify and compare spatial economic disparities for a study area in North Rhine-Westphalia (NRW), Germany. Subsequently, based on these variables, a multivariate clustering approach was conducted for a demonstration area in Dortmund. In the result, it was obvious that the spatially disaggregated data allow more detailed insight into spatial patterns of socioeconomic attributes than the coarser data related to postal code polygons.

Transition Experiences of Indian Nurses Into Australian Mental Health System

2021 ◽  
pp. 104365962110263
Author(s):  
Bindu Joseph ◽  
Michael Olasoji ◽  
Cheryl Moss ◽  
Wendy Cross
Keyword(s):  
Mental Health ◽  
Thematic Analysis ◽  
Mental Health System ◽  
Methodological Approach ◽  
Hermeneutic Phenomenology ◽  
Dual Culture ◽  
The Past ◽  
Transition Experience ◽  
Depth Interview ◽  
Insight Into

Introduction: In the past decade, there has been an influx of migrant nurses from India to Australia. Migrant professionals have specific transition needs associated with working in Mental Health (MH). This study aimed to explore the transition experience of overseas trained nurses from India working in Australian MH settings. Method: Hermeneutic phenomenology was the methodological approach used in the study. The participants ( N = 16) were overseas trained nurses from India. Data were collected through in-depth interview and analysed using thematic analysis. Results: The findings resulted in the identification of the following themes: (1) Living in dual culture, (2) Loneliness, (3) Discrimination, and (4) Feeling incomplete. Discussion: It is evident that the transition to work in MH in Australia was a journey of mixed experiences. While certain findings of this study are comparable with experiences of migrant nurses in other settings, it provides insight into those that are working in MH.

scholarly journals Student engagement with sustainability: understanding the value–action gap

2014 ◽  
Vol 15 (4) ◽  
pp. 404-417 ◽  
Author(s):  
Gareth Chaplin ◽  
Paul Wyton
Keyword(s):  
Design Methodology ◽  
Strong Association ◽  
Structured Interviews ◽  
Online Questionnaire ◽  
Sustainable Living ◽  
Content Type ◽  
Lack Of Information ◽  
Significant Barrier ◽  
The Uk ◽  
Insight Into

Purpose – The purpose of this paper is to present the findings of research that aimed to determine what university students living in Unite accommodation in the UK understand about the concept of sustainable living. It considers what barriers they perceive to be standing in the way of following sustainable living practices. In particular, the research aimed to explore any value–action gap for the student population with a view to informing future actions to help close any gap. Design/methodology/approach – The study was completed through an online questionnaire survey of students living in halls of residence operated by provider The Unite Group Plc. The survey informed semi-structured interviews and focus groups that explored the issues raised in greater detail. Findings – It was found that students living in Unite properties believed sustainable living to be important, yet levels of understanding were very low and there appeared to be a wide value–action gap. Reasons for this are varied; however, an unexpected theme emerged around the association of effort and importance. There was a very strong association between sustainable living and recycling, which, therefore, saw the lack of adequate recycling facilities as a significant barrier to sustainable living. There were also issues around a lack of information, cost and respondents’ flatmates as further barriers. However, the most significant barrier was the displacement of responsibility for sustainable living to other people or organisations. Originality/value – Gaining an insight into the complexity of attitude and behaviour of students with the sustainability agenda will enable understanding that can be applied to activities that promote sustainability.

scholarly journals The Communication of Ideas across Subfields in Political Science

2014 ◽  
Vol 47 (02) ◽  
pp. 468-476 ◽  
Author(s):  
Erin R. Graham ◽  
Charles R. Shipan ◽  
Craig Volden
Keyword(s):  
Network Analysis ◽  
International Relations ◽  
Political Science ◽  
Comparative Politics ◽  
Diffusion Processes ◽  
Methodological Approach ◽  
Journal Articles ◽  
Insight Into ◽  
Shed Light ◽  
Levels Of Integration

ABSTRACTWhat factors inhibit or facilitate cross-subfield conversations in political science? This article draws on diffusion scholarship to gain insight into cross-subfield communication. Diffusion scholarship represents a case where such communication might be expected, given that similar diffusion processes are analyzed in American politics, comparative politics, and international relations. We identify nearly 800 journal articles published on diffusion within political science between 1958 and 2008. Using network analysis we investigate the degree to which three “common culprits”—terminology, methodological approach, and journal type—influence levels of integration. We find the highest levels of integration among scholars using similar terms to describe diffusion processes, sharing a methodological approach (especially in quantitative scholarship), and publishing in a common set of subfield journals. These findings shed light on when cross-subfield communication is likely to occur with ease and when barriers may prove prohibitive.

scholarly journals Oxidative stress: a common denominator in every pathogenesis

2020 ◽  
pp. 202-203
Author(s):  
PA De Valdoleiros
Keyword(s):  
Oxidative Stress ◽  
Signs And Symptoms ◽  
Common Denominator ◽  
Biological Processes ◽  
Medical Doctors ◽  
Insight Into

As medical doctors, we are expected to be the champions of health and the slayers of disease. Essential to the success of this battle is an understanding of, and insight into, the chemistry that allows you the opportunity of reading this article. It is not sufficient to name the parts. It is imperative that we understand how the parts work, the processes that lead to malfunctions, and how these malfunctions appear as signs and symptoms to which we eventually give a label. We have concentrated on the results of biological processes gone awry. It is time to deal with the causes.

scholarly journals Scientific methods of public administration in migration research

2020 ◽  
Vol 68 (2) ◽  
pp. 109-122
Author(s):  
András László Szabó
Keyword(s):  
Public Administration ◽  
Research Methods ◽  
Methodological Approach ◽  
Point Of View ◽  
Scientific Methods ◽  
Migration Research ◽  
Independent Field ◽  
The Subject ◽  
The Given ◽  
Insight Into

In my study, I list the directions of migration research, all from the point of view of public administration science. The emergence of migration research as an independent field is necessitated by the growth of migration. Researchers are researching the phenomenon of migration from their own field. Public administration uses a multidisciplinary, i.e. methodological approach of several disciplines, all of which is determined by the knowledge and nature of the subject of the given research. In the following, I review and evaluate the methodological directions of the research, which can provide an answer to a segment of the phenomenon of migration. The study gives an insight into research methods for migration research. It is not intended to present specific examples and procedures. At the end of the study, in possible directions, beyond the science of public administration, I list the organizations and faculties that are able to use the methodology of migration research.

Sign in / Sign up

Export Citation Format

Share Document