scholarly journals Polyphenolic and molecular variation in Thymus species using HPLC and SRAP analyses

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Danial Sarfaraz ◽  
Mehdi Rahimmalek ◽  
Ghodratollah Saeidi
Keyword(s):  
Hplc Analysis ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Model Systems ◽  
Total Phenolic ◽  
Species Structure ◽  
Components Analysis ◽  
Amova Analysis ◽  
Phenolic And Flavonoid ◽  
Β Carotene

AbstractIn the present research, inter and intra genetic variability of 77 accessions belonging to 11 Thymus species were assessed using eight SRAP primer combinations. High polymorphism (98.3%) was observed in the studied species. The cluster analysis classified Thymus species into five main groups. According to molecular variance (AMOVA) analysis, 63.14% of total genetic variation was obtained within the species, while 36.86% of variation was observed among species. STRUCTURE analysis was also performed to estimate the admixture of species. For instance, T. carmanicus and T. transcaspicus revealed high admixtures. HPLC analysis also demonstrated the presence of rosmarinic acid (32.3–150.7 mg/100 g DW), salvianolic acid (8–90 mg/100 g DW), and cinnamic acid (1.7–32.3 mg/100 g DW) as major phenolic acids, as well as apigenin, epicatechin, and naringenin as the major flavonoids. The highest phenolic and flavonoid contents were detected in T. transcaspicus (37.62 mg gallic acid equivalents (GAE) g−1 DW) and T. vulgaris (8.72 mg quercetin equivalents (QE) g−1 DW), respectively. The antioxidant properties and total phenolic of Thymus species were examined using DPPH and β-carotene-linoleic acid model systems and consequently T. vulgaris and T. pubescens were detected with the highest and the lowest antioxidant activities respectively. Cluster and principal Components Analysis (PCA) of the components classified the species in to three groups. Finally, similarity within some species was observed comparing molecular and phytochemical markers. For instance, T. vulgaris separated from other species according to major polyphenolic profiles and molecular analyses, as well as T. transcaspicus, T. carmanicus, and T. fedtschenkoi that were clustered in the same groups.

scholarly journals Polyphenolic Contents and Antioxidant Properties of Different Grape (V. vinifera,V. labrusca, andV. hybrid) Cultivars

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Shivraj Hariram Nile ◽  
S. H. Kim ◽  
Eun Young Ko ◽  
Se Won Park
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Coumaric Acid ◽  
Grape Skin ◽  
Phenolic Contents ◽  
Grape Skins ◽  
Phenolic And Flavonoid

The polyphenolic contents and the antioxidant activity of the skins and pulps of different grape cultivars were estimated using HPLC and DPPH antioxidant assay, respectively. The phenolics and flavonoids identified were quercetin, kaempferol, caffeic acid, p-coumaric acid, cinnamic acid, and (−)-epicatechin. The total phenolic contents were found to be the highest in the grape skin of Flouxa (>400 mg/100 g), followed by Campbell Early and Tamnara (>300 mg/100 g), and then by Red Globe and Ruby Seedless (>250 mg/100 g), and the total phenolic content was the lowest in Italia and Delaware (<60 mg/100 g). The antioxidant activities of the grape extracts varied from 12.5% (Ruby Seedless) to 60.2% (Hongiseul) for skins, whereas the antioxidant activities of the grape extracts varied from 35.4% (Campbell Early) to 84.5% (Hongiseul) for pulps. The grape pulps have stronger antioxidant activities than those of the grape skins. Our results suggest that the phenolic and flavonoid contents in extracts of grape skins and pulps showed statistically significant correlations with the free radical scavenging activity.

scholarly journals Total Phenolic Contents and Antioxidant Properties of Algerian Alkanna tinctoria aerial part Extracts

2020 ◽  
Vol 10 (5) ◽  
pp. 39-44
Author(s):  
Thoraya Guemmaz ◽  
Lekhmici Arrar ◽  
Abderrahmene Baghiani
Keyword(s):  
Antioxidant Activity ◽  
Aerial Part ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Iron Chelation ◽  
Total Phenolic ◽  
Total Polyphenols ◽  
Alkanna Tinctoria ◽  
Dried Extract ◽  
Β Carotene

Objective: Evaluation of the polyphenols, flavonoids and tannins contents of different aqueous extracts of the aerial part of Alkanna tinctoria and their antioxidant activities.  Methodology: Three extracts;  decoction, macerate and infusion of the aerial part of Alkanna tinctoria were prepared. The content of total polyphenols, flavonoids and tannins of the extracts was carried out and antioxidant activity was assessed using DPPH (2,2’-diphenyl-1-picryl hydrazyl), β-carotene bleaching and iron chelation tests. Results : The results showed that Infusion (Inf) was the richest in total polyphenols (189.66 ± 30.77 mg EGA / g of dried extract) and flavonoids (26.80 ± 8.37 mg EQ / g of dried extract). While the content of tannins is almost the same for Dec and Inf (40.27 ± 15.00, 40.25 ± 18.27 mg ETA / g of dried extract). Moreover, the two extracts Inf (IC50 of 0.09 ± 0.0015 mg / ml) and Dec (IC50 of 0.17 ± 0. 031 mg / ml) showed a very strong anti-radical activity towards the radical DPPH. In the β-carotene bleaching test, Inf and Dec showed a significant inhibitory activity of peroxidation with values ​​of 100% and 99.8% and are more effective compared to BHT (98.15%). Dec and Mac showed strong chelating activity with IC50s of 0.003 ± 0.0002 mg / ml and 0.005 ± 0.00008 mg / ml respectively. These chelating activities remain higher than EDTA. Conclusion: It may be concluded that the extracts of the aerial part of Alkanna tinctoria exhibit significant both content of polyphenols and antioxidant activity. Keywords: Alkanna tinctoria, polyphenols, flavonoids, antioxidant activity.

In vitro Cytotoxic Effect, Hemolytic, and Antioxidant Activities of the Algerian Species Nonea vesicaria Rchb.

2020 ◽  
Vol 16 (8) ◽  
pp. 1197-1204 ◽  
Author(s):  
Soumia Mouffouk ◽  
Chaima Mouffouk ◽  
Chawki Bensouici ◽  
Hamada Haba
Keyword(s):  
Cytotoxic Effect ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
A Value ◽  
Brine Shrimp Lethality Assay ◽  
Cytotoxic Compounds ◽  
Phenolic And Flavonoid ◽  
Β Carotene

Objective: The aim of this study is the estimation of total phenolic and flavonoid contents and the evaluation of cytotoxic, hemolytic and antioxidant activities of the methanolic extract obtained from the species Nonea vesicaria (L.) Rchb. Methods: The total phenolic and flavonoid contents were quantified by Folin-Ciocalteu and trichloroaluminum methods, respectively. The cytotoxic effect was tested by Brine shrimp lethality assay and the hemolytic activity was assessed by spectrophotometric test on human erythrocytes. Moreover, the antioxidant activity was determined by seven different techniques. Results: The phytochemical screening revealed the presence of many classes of secondary metabolites, a moderate level of polyphenols, and a low content of flavonoids. The methanolic extract showed a significant cytotoxic effect with a value of LC50 at 35.7±0.5 μg/mL and induced hemolysis in a dosedependent manner with a value of EC50 at 175.6±0.08 μg/mL. The results of antioxidant activities indicated an important effect on nonpolar systems especially in ferric thiocyanate test and β-carotene bleaching inhibition assay. Conclusion: The methanolic extract of N. vesicaria could constitute an important source of antioxidant and cytotoxic compounds but prudent use is recommended in order to reduce the adverse effects related to the possible hemolysis.

scholarly journals Chemical Composition of Leaves and Hull from Pistacia Vera L. an Evaluation of Phenolic Content and Antioxidant Properties of their Extracts

2020 ◽  
Author(s):  
Manel Elakremi ◽  
Leyre Sillero ◽  
Lazher Ayed ◽  
Jalel Labidi ◽  
Younes Moussaoui
Keyword(s):  
Phenolic Content ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Chemical Characterization ◽  
Total Phenolic ◽  
Pistacia Vera ◽  
Phenolic And Flavonoid ◽  
By Products ◽  
Comprehensive Study

Abstract The pistachio industry in Tunisia generates a high amount of waste every year due to the large pistachio production. This, together with the good properties of the compounds that can be extracted from these by-products, makes it necessary a comprehensive study of their potential. Because of that, the aim of this work was to analyze the chemical characterization of male and female leaves and hull of Pistacia vera L. and their ethanol-water extracts. With that purpose, the total phenolic and flavonoid contents of the extracts were determined and their antioxidant activities were investigated using DPPH, ABTS and FRAP methods. The total phenolic content varied from 218to533mg GAE/g DE, and the total flavonoid content varied from119to 397 mg CE/g DE. In general, the antioxidant activity showed significant values. The DPPH assay and the ABTS assay were in the range of 332–704 mg TE/g DE and 427and 1394 mg TE/g DE, respectively .In the case of FRAP, male leaves extract had the best result, being the TE value 808 mg TE/g DE. All the extracts were rich in phenolic compounds and possess valuable antioxidant activities. Therefore, leaves and hull of Pistacia vera L. could be used as cheap natural antioxidant in different applications.

scholarly journals Antioxidant Activities and Total Phenolic and Flavonoid Contents in Three Indigenous Medicinal Vegetables of North-east India

2012 ◽  
Vol 7 (8) ◽  
pp. 1934578X1200700
Author(s):  
Jyotirekha G. Handique ◽  
Manas Pratim Boruah ◽  
Dipika Kalita
Keyword(s):  
Hplc Analysis ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Leafy Vegetables ◽  
Total Phenolic ◽  
Methanol Extracts ◽  
North East India ◽  
North East ◽  
Main Components ◽  
Phenolic And Flavonoid

Antioxidant activities of the n-hexane, ethyl acetate and methanol extracts of three indigenous leafy vegetables of North East India viz., Polygonum microcephallum, Oxalis corniculata and Portulaca oleraceae were measured by spectroscopic methods using the 1, 1- diphenyl-2-picrylhydrazyl (DPPH•) radical assay and xanthine/xanthine oxidase assay. The total phenolic and total flavonoid contents of each extract were also measured to assess their effect on the antioxidant activity. It was observed that the methanol extracts of all the species showed the highest antioxidant activities and high values for total phenolic and flavonoid contents. A strong correlation between the antioxidant activities and the total phenolic content was observed for the three vegetables. It indicates that phenolics are one of the main components responsible for the antioxidant behavior of vegetables. HPLC analysis showed the presence of a number of identified phenolic compounds.

scholarly journals PHYTOCHEMICAL AND ANTIOXIDANT PROPERTIES OF VARIOUS EXTRACTS OF MICHELIA CHAMPACA LEAVES

2019 ◽  
pp. 56-61
Author(s):  
PUSHPA RUWALI ◽  
MAMTA ADHIKARI ◽  
SUBHANSHI SHARMA
Keyword(s):  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Animal Health ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Crude Extracts ◽  
Michelia Champaca ◽  
Phenolic And Flavonoid

Objective: To investigate and compare the phytochemicals, antioxidant activities of various crude extracts from fresh leaves of locally available plant Michelia champaca belonging to family Magnoliaceae. Methods: The present study was designed to perform preliminary phytochemical (qualitative and quantitative) analysis of various extracts. The crude extracts from the leaves of M. champaca were prepared using various organic solvents viz. Methanol (MCMET), Ethanol (MCETH), Hexane (MCHEX), and Chloroform (MCCHLO). In addition, total phenolic (TPC) and total flavonoid (TFC) contents of various extracts were also measured along with the evaluation of the antioxidant capacities using most widely accepted in vitro chemical tests such as DPPH and ABTS radical scavenging assay. Results: The methanolic extract of M. champaca showed the presence of maximum phytochemicals (12) when compared to other solvent extracts viz. Hexane (10), Ethanol (8) and Chloroform(7). The amount of phenolic and flavonoids present in solvents were in the order of Methanol>Hexane>Ethanol>Chloroform. The best antioxidant potential was found in the Methanol extract. The Methanolic extract exhibited an IC50 value of 72.03µg/ml (DPPH) and of 185.21µg/ml (ABTS), respectively for the two radical scavenging assays. Conclusion: Phytochemical analysis of M. champaca (leaves) various extracts showed good amounts of phenolic and flavonoid contents and also exhibited significant antioxidant activity. A positive linear correlation between the antioxidant activities and the total phenolic and flavonoid contents of the plant extracts was observed. Study revealed variations in such reported activities due to different organic extraction solvents used. In overall conclusion, Michelia champaca has good potential as a source of natural antioxidant for animal health and food industry and further relevant studies on similar lines would be worthwhile.

scholarly journals Investigation of antioxidant properties of Withania somnifera (L.) Dunal and influence of physico-chemical properties of soil along the topographic gradients in sub-tropical region of the Indian Himalaya

2017 ◽  
Vol 9 (3) ◽  
pp. 407
Author(s):  
Kundra R ◽  
S S Samant ◽  
SK Nandi ◽  
RK Sharma
Keyword(s):  
Medicinal Plant ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Chemical Properties ◽  
Total Phenolic ◽  
Tropical Region ◽  
Indian Himalaya ◽  
Physico Chemical ◽  
Phenolic And Flavonoid ◽  
Properties Of Soil

<p>A high value medicinal plant <em>W. somnifera </em>L. Indian Ginseng; Ashwagandha investigated for the antioxidant and influence of physico-chemical properties of soil on antioxidant  along the topographical gradients in Kullu Valley, North Western Indian Himalaya. Total phenolic and flavonoid contents in methanol extracts of fruits, leaves, stems and roots were quantified and their antioxidant activities were evaluated using the <em>in-vitro</em> assays such as DPPH, ABTS and FRAP. The study revealed that fruits, leaves, stems and roots of <em>W. somnifera</em> were potent source of natural phenolics and flavonoids and possesses antioxidant activities, which were significantly affected by the changes in altitude (p ≤0.05). Total phenolic and flavonoid contents in different parts of <em>W. somnifera</em> showed significant correlations with soil properties. The study conclude that changes in topographical gradients particularly altitude influenced antioxidant activities of <em>W. somnifera</em> and suggest that antioxidant rich population of a desired medicinal plant should be identified for their conservation management and sustainable utilization in pharmaceutical and food industries.</p><p> </p>

scholarly journals Lonicera iberica M. Bieb.: Investigation Antioxidant Activity and Bioactive Chemicals

2021 ◽  
Vol 9 (6) ◽  
pp. 1124-1128
Author(s):  
Fatma Ergün
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Phenolic Substance ◽  
Amount Of Substance ◽  
Phenolic And Flavonoid

In this study, it was investigated the total amounts of phenolic and flavonoid substances and antioxidant activities in different solvent extracts of Lonicera iberica M. Bieb. wild fruit. Total phenolic contents of the extracts were determined as equivalent to gallic acid using Folin-Ciocalteu reagent, and total flavonoid contents as equivalent to quercetin by aluminium nitrate method. In addition, the antioxidant properties of the extracts were determined using free radical scavenging (DPPH) and reducing power (FRAP) methods. The amount of total phenolic substance of L. iberica fruits in hexane and methanol extracts was calculated as 30.96 ± 0.67 mg of GAE / g and 23.70 ± 1.56 mg of GAE / g, respectively. In addition, the amount of total flavonoid substance was calculated as 46.50 ± 8.54 mg of QE / g and 42.09 ± 2.58 mg of QE / g, respectively. It was determined that DPPH radical scavenging activity correlated with total phenolic and flavonoid amount of substance, and L. iberica had a strong antioxidant effect. It is predicted that our study will shed light on new researches, since it is the first study done with L. Iberica fruits in this field.

scholarly journals HPLC-UV profile of Genista ulicina Spach. (Fabaceae) extracts and in vitro antioxidant activity

2016 ◽  
Vol 8 (3) ◽  
pp. 398 ◽  
Author(s):  
Kaoutar Chebbah ◽  
Eric Marchioni ◽  
Djamel Sarri ◽  
Ahmed Menad ◽  
Ratiba Mekkiou ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Colorimetric Method ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Uv Detector ◽  
Etoac Extract ◽  
Phenolic And Flavonoid ◽  
Β Carotene

<p>To perform a qualitative and quantitative analysis of the phenolic and flavonoid contents and evaluate the antioxidant activity of ethyl acetate (EtOAc) and <em>n</em>-butanol (<em>n</em>-BuOH) extracts of the aerial parts of <em>Genista ulicina </em>Spach. from Algeria.<strong> </strong>The qualitative analysis of plant extracts was carried out by RP-HPLC using UV detector, whereas the quantification of total phenolic and flavonoid contents was completed according to the Folin-Ciocalteu procedure and aluminium chloride colorimetric method respectively. To evaluate the extract's antioxidant activity, Two in vitro antioxidant tests were employed: DPPH and β-carotene bleaching assay. The HPLC/DAD chromatogram showed several peaks indicating the presence of phenolic acids, flavonoids and isoflavonoids in both extracts. The total phenolic content (TPC) ranged from 62.56 and 50.45 mgGAE/g extract, while the total flavonoids content varied between 53.1 and 48.4 mgQE/g extract for EtOAC and <em>n</em>-BuOH respectively. EtOAc extract showed a maximum inhibition value (78.15%) at 150µg/mL using DPPH test and highest antioxidative power (82.42%) using β-carotene bleaching assay comparing with standards. The HPLC-UV analysis showed the richeness of both extracts in phenolic and flavonoid contents. The EtOAc<em> </em>extract exhibited good antioxidant activities comparing to the <em>n</em>-BuOH extract. Thus <em>Genista ulicina</em> could be indicated as a plant of phytopharmaceutical importance.<strong></strong></p>

scholarly journals Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
E. S. Prasedya ◽  
A. Frediansyah ◽  
N. W. R. Martyasari ◽  
B. K. Ilhami ◽  
A. S. Abidin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Particle Sizes ◽  
Epicatechin Gallate ◽  
Particle Size Reduction ◽  
Phytochemical Composition

AbstractSample particle size is an important parameter in the solid–liquid extraction system of natural products for obtaining their bioactive compounds. This study evaluates the effect of sample particle size on the phytochemical composition and antioxidant activity of brown macroalgae Sargassum cristaefolium. The crude ethanol extract was extracted from dried powders of S.cristeafolium with various particle sizes (> 4000 µm, > 250 µm, > 125 µm, > 45 µm, and < 45 µm). The ethanolic extracts of S.cristaefolium were analysed for Total Phenolic Content (TPC), Total Flavonoid Content (TFC), phenolic compound concentration and antioxidant activities. The extract yield and phytochemical composition were more abundant in smaller particle sizes. Furthermore, the TPC (14.19 ± 2.08 mg GAE/g extract to 43.27 ± 2.56 mg GAE/g extract) and TFC (9.6 ± 1.8 mg QE/g extract to 70.27 ± 3.59 mg QE/g extract) values also significantly increased as particle sizes decreased. In addition, phenolic compounds epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), and Epigallocatechin gallate (EGCG) concentration were frequently increased in samples of smaller particle sizes based on two-way ANOVA and Tukey’s multiple comparison analysis. These results correlate with the significantly stronger antioxidant activity in samples with smaller particle sizes. The smallest particle size (< 45 µm) demonstrated the strongest antioxidant activity based on DPPH, ABTS, hydroxyl assay and FRAP. In addition, ramp function graph evaluates the desired particle size for maximum phytochemical composition and antioxidant activity is 44 µm. In conclusion, current results show the importance of particle size reduction of macroalgae samples to increase the effectivity of its biological activity.

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