scholarly journals The absence of the caffeine synthase gene is involved in the naturally decaffeinated status of Coffea humblotiana, a wild species from Comoro archipelago

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nathalie Raharimalala ◽  
Stephane Rombauts ◽  
Andrew McCarthy ◽  
Andréa Garavito ◽  
Simon Orozco-Arias ◽  
...  

AbstractCaffeine is the most consumed alkaloid stimulant in the world. It is synthesized through the activity of three known N-methyltransferase proteins. Here we are reporting on the 422-Mb chromosome-level assembly of the Coffea humblotiana genome, a wild and endangered, naturally caffeine-free, species from the Comoro archipelago. We predicted 32,874 genes and anchored 88.7% of the sequence onto the 11 chromosomes. Comparative analyses with the African Robusta coffee genome (C. canephora) revealed an extensive genome conservation, despite an estimated 11 million years of divergence and a broad diversity of genome sizes within the Coffea genus. In this genome, the absence of caffeine is likely due to the absence of the caffeine synthase gene which converts theobromine into caffeine through an illegitimate recombination mechanism. These findings pave the way for further characterization of caffeine-free species in the Coffea genus and will guide research towards naturally-decaffeinated coffee drinks for consumers.

Water ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 459
Author(s):  
Ignacio Cazcarro ◽  
Albert E. Steenge

This article originates from the theoretical and empirical characterization of factors in the World Trade Model (WTM). It first illustrates the usefulness of this type of model for water research to address policy questions related to virtual water trade, water constraints and water scarcity. It also illustrates the importance of certain key decisions regarding the heterogeneity of water and its relation to the technologies being employed and the prices obtained. With regard to WTM, the global economic input–output model in which multiple technologies can produce a “homogeneous output”, it was recently shown that two different mechanisms should be distinguished by which multiple technologies can arise, i.e., from “technology-specific” or from “shared” factors, which implies a mechanism-specific set of prices, quantities and rents. We discuss and extend these characterizations, notably in relation to the real-world characterization of water as a factor (for which we use the terms technology specific, fully shared and “mixed”). We propose that the presence of these separate mechanisms results in the models being sensitive to relatively small variations in specific numerical values. To address this sensitivity, we suggest a specific role for specific (sub)models or key choices to counter unrealistic model outcomes. To support our proposal we present a selection of simulations for aggregated world regions, and show how key results concerning quantities, prices and rents can be subject to considerable change depending on the precise definitions of resource endowments and the technology-specificity of the factors. For instance, depending on the adopted water heterogeneity level, outcomes can vary from relatively low-cost solutions to higher cost ones and can even reach infeasibility. In the main model discussed here (WTM) factor prices are exogenous, which also contributes to the overall numerical sensitivity of the model. All this affects to a large extent our interpretation of the water challenges, which preferably need to be assessed in integrated frameworks, to account for the main socioeconomic variables, technologies and resources.


1997 ◽  
Vol 1 (1) ◽  
pp. 52-65
Author(s):  
Hye-Joon Yoon

Area studies, as a newly fashionable field of academic research, needs to recognize its less likely precedents if it is going to secure for itself a fresh start. The question of “desire” is relevant here because it indicates the less value-free aspects in its genealogy. As shown in Emma Bovary's embellished representation of Paris at her provincial home, an understanding of an area often reflects the particular needs and desires of the one who understands that area. Such restricted and restricting views of an area repeats itself outside the world of literary fictions, as is shown by the example of Guizot's picture of Europe in which his own country is given a privileged place as the very center of Western civilization itself. An instructive case showing the thin line between the projected desire of one who strives to know a geographical area and the scientific purity of the labor itself is further offered by Napoleon Bonaparte's heavy reliance on Orientalist scholarship in his invasion of Egypt. Moving further east from Egypt to China, we witness the denigrating remarks on China made by the great German thinkers of the past century, Hegel and Weber. Although their characterization of Chinese culture could find echoes in unbiased empirical research, they reveal all the same the trace of Europeans' desire to affirm their superiority over the supposedly inferior and false civilization of the East. Similarly, the Americans who divided the Korean peninsular at the 38th Parallel, with unquestioning confidence in their knowledge of the area and in the justice of their action, rightfully deserve their place in the tradition of Western area studies of serving the needs to dominate, control and exploit an objectified overseas territory. He assumed that words had kept their meaning, that desires still pointed in a single direction, and that ideas retained their logic; and he ignored the fact that the world of speech and desires has known invasions, struggles, plundering, disguises, ploys. From these elements, however, genealogy retrieves an indispensable restraint: it must record the singularity of events outside of any monotonous finality; it must seek them in the most unpromising places, in what we tend to feel is without history—in sentiments, love, conscience, instincts; it must be sensitive to their recurrence, not in order to trace the gradual curve of their evolution, but to isolate the different scenes where they engaged in different roles. — Michel Foucault, “Nietzsche, Genealogy, History” (Foucault 139–40).


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Christopher Alan Smith

AbstractThe basidiomycete fungus Lentinula novae-zelandiae is endemic to New Zealand and is a sister taxon to Lentinula edodes, the second most cultivated mushroom in the world. To explore the biology of this organism, a high-quality chromosome level reference genome of L. novae-zelandiae was produced. Macrosyntenic comparisons between the genome assembly of L. novae-zelandiae, L. edodes and a set of three genome assemblies of diverse species from the Agaricomycota reveal a high degree of macrosyntenic restructuring within L. edodes consistent with signal of domestication. These results show L. edodes has undergone significant genomic change during the course of its evolutionary history, likely a result of its cultivation and domestication over the last 1000 years.


2020 ◽  
Vol 40 (6) ◽  
Author(s):  
Luciano Nobuhiro Aoyagi ◽  
Yukie Muraki ◽  
Naoki Yamanaka

Abstract Phakopsora pachyrhizi is an obligatory biotrophic fungus that causes Asian soybean rust (ASR) disease. ASR control primarily involves chemical control and the use of resistant soybean cultivars carrying an Rpp (resistance to P. pachyrhizi) gene. This study aimed to characterize the ASR resistance of three soybean Asian landraces. By screening the world core collection (WC) of soybean, which consists of 80 varieties, three landraces were identified in Southeast Asia as resistant to ASR. Genetic mapping using the F2 population derived from a cross with an ASR-susceptible variety, BRS 184, indicated that KS 1034 (WC2) has ASR resistance conferred by a single dominant resistance gene, mapped on chromosome 18, in the same region where Rpp1 was mapped previously. The BRS 184 × WC61 (COL/THAI/1986/THAI-80) F2 population, on the other hand, showed an ASR resistance locus mapped by quantitative trait locus analysis on chromosome 6, in the region where the resistance conferred by PI 416764 Rpp3 resides, with a logarithm of the odds score peak at the same position as the marker, Satt079, while the BRS 184 × WC51 (HM 39) population showed the resistance to ASR allocated between Satt079 and Sat_263 markers, also in the region where Rpp3 was mapped previously. Both WC51 and WC61 have the same infection profile as FT-2 and PI 462312 when tested against the same ASR isolate panel. These three WCs can be used in MAS programs for introgression of Rpp1 and Rpp3 and the development of ASR-resistant cultivars in the breeding program.


Author(s):  
Марина Михайловна Молчанова ◽  
Патимат Абдулаевна Лекова

Исследуется кризис вербальности контента в интернет-дискурсе, его активная визуализация, связанная с новыми открытиями в области компьютерных технологий. Цель работы - дать полиаспектную характеристику этому явлению и обозначить перспективы его влияния на речемыслительную деятельность подрастающего поколения, составляющего большую часть пользователей социальных сетей. Актуальность данной проблематики обусловлена обеспокоенностью филологической общественностью тем, что мир становится все менее лингвистическим. Новизна заключается в том, что впервые интернет-дискурс рассматривается в контексте кризиса вербальности контента. При таких темпах визуализации медиатекстов вполне вероятно обострение кризиса вербальности, последствия которого могут негативно сказаться на мыслетворческой деятельности у подрастающего поколения, которая возможна только при высокой языковой компетенции. The paper examines the crisis of content verbality in the Internet discourse, its active visualization associated with new discoveries in the field of computer technology. The aim of the work is to give a multi-aspect characterization of this phenomenon and outline the prospects of its influence on the speech-thinking activity of the younger generation, which makes up the majority of users of social networks. The relevance of this issue is due to the concern of the philological community that the world is becoming less and less linguistic. The novelty lies in the fact that for the first time Internet discourse is considered in the context of the crisis of content verbality. At such a rate of visualization of media texts, it is likely that the crisis of verbality will aggravate, the consequences of which can negatively affect the thinking activity of the younger generation, which is possible only with high linguistic competence.


Biology ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 105
Author(s):  
Olga M. Zając ◽  
Stefan Tyski ◽  
Agnieszka E. Laudy

An increase of nosocomial infections caused by Stenotrophomonas maltophilia strains has recently been observed all over the world. The isolation of these bacteria from the blood is of particular concern. In this study we performed the phenotypic and genotypic characterization of 94 S. maltophilia isolates, including isolates from patients hospitalized in a tertiary Warsaw hospital (n = 79) and from outpatients (n = 15). All isolates were found to be susceptible to trimethoprim-sulfamethoxazole and minocycline, while 44/94 isolates demonstrated a reduction in susceptibility to levofloxacin. A large genetic variation was observed among the isolates tested by pulsed-field gel electrophoresis. A clonal relationship with 100% similarity was observed between isolates within two sub-pulsotypes: the first included nine bloodstream isolates and the second involved six. Multilocus sequence typing showed two new sequence types (ST498 and ST499) deposited in public databases for molecular typing. Moreover, the presence of genes encoding ten different efflux pumps from the resistance-nodulation-division family and the ATP-binding cassette family was shown in the majority of the 94 isolates. The obtained knowledge about the prevalence of efflux pump genes in clinical S. maltophilia strains makes it possible to predict the scale of the risk of resistance emergence in strains as a result of gene overexpression.


PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10503
Author(s):  
Jessica Wilkie ◽  
Timothy C. Cameron ◽  
Travis Beddoe

Fasciola hepatica is the causative agent of fasciolosis, an important disease of humans and livestock around the world. There is an urgent requirement for novel treatments for F. hepatica due to increasing reports of drug resistance appearing around the world. The outer body covering of F. hepatica is referred to as the tegument membrane which is of crucial importance for the modulation of the host response and parasite survival; therefore, tegument proteins may represent novel drug or vaccine targets. Previous studies have identified a profilin-like protein in the tegument of F. hepatica. Profilin is a regulatory component of the actin cytoskeleton in all eukaryotic cells, and in some protozoan parasites, profilin has been shown to drive a potent IL-12 response. This study characterized the identified profilin form F. hepatica (termed FhProfilin) for the first time. Recombinant expression of FhProfilin resulted in a protein approximately 14 kDa in size which was determined to be dimeric like other profilins isolated from a range of eukaryotic organisms. FhProfilin was shown to bind poly-L-proline (pLp) and sequester actin monomers which is characteristic of the profilin family; however, there was no binding of FhProfilin to phosphatidylinositol lipids. Despite FhProfilin being a component of the tegument, it was shown not to generate an immune response in experimentally infected sheep or cattle.


2012 ◽  
pp. 47-50
Author(s):  
Katalin Kalmár

Tourism is one of the biggest and mostly increasing branch all over the world. Since it is getting more and more significant in Hungary as well in this article I analyze the turnover of Hungarian tourism on the basis of guest nights spent on commercial lodgings. I examine its changes in time and its territorial spreading. Finally I explore the health touristic volume, because it is one of the most important touristic product of Hungary and the North Plain Region and the infrastructure based upon preserves unique opportunities both for health and wellness tourism.


Author(s):  
Daniela Medrano-David ◽  
Aura Maria Lopera ◽  
Martha Elena Londoño ◽  
Pedronel Araque-Marín

The occurrence of bone-related disorders and diseases has increased dramatically in recent years around the world. Demineralized bone matrix (DBM) has been widely used as a bone implant due to its osteoinduction and bioactivity. However, the use of DBM is limited because it is a particulate material, which makes it difficult to manipulate and implant with precision, in addition, these particles are susceptible to migrate to other sites. To address this situation, DBM is commonly incorporated into a variety of carriers. An injectable scaffold has advantages over bone grafts or preformed scaffolds, such as the ability to flow and fill the bone defect. The aim of this research is to develop a DBM carrier with such viscoelastic properties to obtain an injectable bone substitute (IBS). The DBM carrier developed consisted of a PVA/glycerol network cross-linked with borax and reinforced with CaCO3 as a pH neutralizer, porosity generator, and source of Ca. The physicochemical properties were determined by the injectability test, FTIR, SEM, and TGA. Porosity, degradation, bioactivity, possible cytotoxic effect, and proliferation in osteoblasts were also determined. The results show that the developed material has great potential to be used in bone tissue regeneration


Author(s):  
J. Lázár ◽  
Gy. D. Bisztray

Viruses and viroids are submicroscopic infectious particles which can cause disease symptoms on grapevine. These parasites are depending completely on the energy metabolism of the plant cell. To enter the host cell plant viruses depend on injuries or on transmission via invertebrates (insects, nematodes, etc.). Viruses are classified by many characters including particle morphology, host range and information content of the genome. At present about 70 viruses including 7 viroids infecting grapevine are known. In single or mixed infections they are potentially detrimental to the quality and quantity of grape production in any growing area of the world. Some viruses can cause severe economic damage in vineyards. In Hungary many important viruses and viroids have been detected in grape. This review summarises characteristics of viruses and the results of detection and characterization of virus and virus like diseases of grapevine in Hungary. The identification of the causal agent, its transmission, geographical distribution and the development of the diagnostic methods are also discussed.


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