scholarly journals The microbial profile of a tissue necrosis affecting the Atlantic invasive coral Tubastraea tagusensis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Aline Aparecida Zanotti ◽  
Gustavo Bueno Gregoracci ◽  
Marcelo Visentini Kitahara
Keyword(s):  
Coral Species ◽  
Amplicon Sequencing ◽  
Rocky Reef ◽  
Northern Coast ◽  
Tissue Necrosis ◽  
Microbial Succession ◽  
Microbial Profile ◽  
Diseased Coral ◽  
The Relationship ◽  
Generation Sequencing

AbstractThe Southwestern Atlantic rocky reef ecosystems are undergoing significant changes due to sun-corals (Tubastraea tagusensis and T. coccinea) invasion. At Búzios Island, on the northern coast of São Paulo State, where the abundance of T. tagusensis is particularly high, some colonies are displaying tissue necrosis, a phenomenon never reported for this invasive nor any other azooxanthellate coral species. Using next-generation sequencing, we sought to understand the relationship between T. tagusensis tissue necrosis and its microbiota. Thus, through amplicon sequencing, we studied both healthy and diseased coral colonies. Results indicate a wide variety of bacteria associated with healthy colonies and an even higher diversity associated with those corals presenting tissue necrosis, which displayed nearly 25% more microorganisms. Also, as the microbial community associated with the seven healthy colonies did not alter composition significantly, it was possible to verify the microbial succession during different stages of tissue necrosis (i.e., initial, intermediate, and advanced). Comparing the microbiome from healthy corals to those in early tissue necrosis suggests 21 potential pathogens, which might act as the promoters of such disease.

MICROBIOMA: AMIGO O ENEMIGO EN PACIENTES CON COVID - 19

SCIENTIARVM ◽  
2015 ◽  
Vol 1 (1) ◽  
pp. 15-21
Author(s):  
Katherine Milagros Quispe Medina ◽  
◽  
Angel Sixto Mamani Ruelas ◽  
Brenda Jasmin Alvarez Vera ◽  
Yasmin Yessenia Silvestre Gutierrez ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Gastrointestinal Tract ◽  
Molecular Biology ◽  
Gut Microbiome ◽  
Next Generation ◽  
Multiple Factors ◽  
Lung Microbiome ◽  
The Relationship ◽  
Generation Sequencing ◽  
Clinical Cases

The research of the microbiome concerning various diseases has grown in the last ten years due to the advances in molecular biology and next-generation sequencing, finding interactions with various pathologies. The new coronavirus 19 (SARS-COV 2) pandemic has aroused interest in the study of multiple factors that could influence in the development of symptoms mainly due to the interaction of the microbiome whether of the respiratory or gastrointestinal tract finally in the prognosis. Therefore, in this study, we focus on reviewing and analyzing the current bibliography of research and clinical cases about the relationship between the lung and gut microbiome and COVID-19, highlighting its effect on infected patients, aiming to contribute to this new line of research. Keywords: Microbiome, COVID-19, SARS-COV 2, gut microbiome, lung microbiome.

Dynamic relationships among tumor, immune response, and microbiota

2019 ◽  
Vol 3 (1) ◽  
pp. 41
Author(s):  
Takuya Tsunoda ◽  
Kazunori Shimada ◽  
Naoki Uchida ◽  
Shinichi Kobayashi ◽  
Yasutsuna Sasaki
Keyword(s):  
Molecular Mechanisms ◽  
Checkpoint Inhibitors ◽  
Advanced Cancer Patients ◽  
Cancer Treatments ◽  
Disease Etiology ◽  
The Past ◽  
Dynamic Relationships ◽  
The Relationship ◽  
Generation Sequencing

Recently, the analysis of microbiota has been of interest not only for the clarification of the molecular mechanisms of disease etiology, but also the discovery of novel strategies for treatment. Following the development of "next-generation" sequencing, novel areas have been discovered in microbiota; however, in oncology, the relationships between microbiota and cancer have not been fully clarified. In recent literature, surprisingly, detection of gut microbiota in tumor issue itself has been reported. Microbiota might play an important role in carcinogenesis. However, this phenomenon is not well understood, and research in this area has just begun. In the past five years, a paradigm shift has occurred in cancer treatment due to immunotherapy. Immunotherapy has made cure possible even in advanced cancer patients with not only melanoma but also non-small cell lung cancer and others. In this review, we discuss the mechanisms of novel immunotherapies, checkpoint inhibitors, and the relationship between microbiota and immunotherapy. It is of significance to clarify this relationship because it may lead to the discovery of predictive markers for immunotherapy and promote clinical efficacy. Finally, we also mention our activities in the construction of a big database for information on immunotherapy and microbiota, which may lead to excellent possibilities of discovering novel strategies for more effective cancer treatments, and may accelerate the alteration of cancers to the classification of chronic nonfatal disease.

Diagnostic Value of the Microbiome in the Bronchoalveolar Fluid of Patients with Lung masses and its Relationship with their Clinical Features

2020 ◽  
Author(s):  
Maosong Ye ◽  
Jian Zhou ◽  
Shengdi Wu ◽  
Jingrong Xia ◽  
Sulan Wei ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Clinical Features ◽  
Area Under The Curve ◽  
Diagnostic Value ◽  
Response To Chemotherapy ◽  
Cancer Subtype ◽  
Bronchoalveolar Fluid ◽  
The Relationship ◽  
Generation Sequencing ◽  
Benign Mass

Abstract Background: Bacterial communities were demonstrated to be correlated with patients with several respiratory diseases. Although some studies have been performed on the composition of the microbiota in lung cancer, the issue has not been fully addressed. Therefore, we characterized the microbiomes of patients with lung cancer and benign mass-like lesions and evaluated the relationship between microbiota and clinical features.Methods: Bronchoalveolar fluid of patients with lung masses was collected and analyzed by 16S rRNA-based next-generation sequencing. Then, the relationships between the composition of the microbiota and clinical features were evaluated. Results: The relative abundance of two genera, Megasphaera and Norank_p_saccharibacteria, and two phyla, Firmicutes and Saccharibacteria, were significantly increased, while one phyla Proteobacteria was decreased in patients with lung cancer. The genera Atoprevotella and 1 phylum, Bacteroidetes, were increased in patients with SCLC, while 1 phylum, Chloroflexi, was more abundant in patients with NSCLC than those with SCLC. Moreover, the patients whose BALF was enriched with the genus Capnocytophaga seemed to have a better response to cisplatin-based chemotherapy. The area under the curve of a combination of two genera (megasphera and norank_p_Saccharibacteria) used to predict lung cancer was 0.803. The area under the curve of the genus Capnocytophaga in predicting the response to chemotherapy was 0.850.Conclusions: There are differences in the composition of the microbiome of patients with lung cancer and those with benign mass-like lesions. The lung microbiota may be used as a biomarker for diagnosing lung cancer and differentiating the cancer subtype and might have an impact in the response to cisplatin-based chemotherapy among patients with lung cancer.

Resource availability drives bacterial succession during leaf-litter decomposition in a bromeliad ecosystem

2020 ◽  
Vol 96 (4) ◽  
Author(s):  
Jean-François Carrias ◽  
Mélanie Gerphagnon ◽  
Héctor Rodríguez-Pérez ◽  
Guillaume Borrel ◽  
Camille Loiseau ◽  
...  
Keyword(s):  
Leaf Litter ◽  
Primary Succession ◽  
Alpha Diversity ◽  
Resource Limitation ◽  
Amplicon Sequencing ◽  
Natural Environments ◽  
Biological Interactions ◽  
Microbial Succession ◽  
Degrading Bacteria ◽  
Bacterial Succession

ABSTRACT Despite the growing number of investigations on microbial succession during the last decade, most of our knowledge on primary succession of bacteria in natural environments comes from conceptual models and/or studies of chronosequences. Successional patterns of litter-degrading bacteria remain poorly documented, especially in undisturbed environments. Here we conducted an experiment with tank bromeliads as natural freshwater microcosms to assess major trends in bacterial succession on two leaf-litter species incubated with or without animal exclusion. We used amplicon sequencing and a co-occurrence network to assess changes in bacterial community structure according to treatments. Alpha-diversity and community complexity displayed the same trends regardless of the treatments, highlighting that primary succession of detrital-bacteria is subject to resource limitation and biological interactions, much like macro-organisms. Shifts in bacterial assemblages along the succession were characterized by an increase in uncharacterized taxa and potential N-fixing bacteria, the latter being involved in positive co-occurrence between taxa. These findings support the hypothesis of interdependence between taxa as a significant niche-based process shaping bacterial communities during the advanced stage of succession.

scholarly journals Meta-Analysis of Microbial Communities in Hot Springs: Recurrent Taxa and Complex Shaping Factors beyond pH and Temperature

2020 ◽  
Vol 8 (6) ◽  
pp. 906 ◽  
Author(s):  
Francisco L. Massello ◽  
Chia Sing Chan ◽  
Kok-Gan Chan ◽  
Kian Mau Goh ◽  
Edgardo Donati ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Metabolic Profile ◽  
Hot Springs ◽  
Meta Analysis ◽  
Extreme Environments ◽  
Alpha Diversity ◽  
Amplicon Sequencing ◽  
Phylogenetic Distance ◽  
Microbial Profile ◽  
The World

The study of microbial communities from extreme environments is a fascinating topic. With every study, biologists and ecologists reveal interesting facts and questions that dispel the old belief that these are inhospitable environments. In this work, we assess the microbial diversity of three hot springs from Neuquén, Argentina, using high-throughput amplicon sequencing. We predicted a distinct metabolic profile in the acidic and the circumneutral samples, with the first ones being dominated by chemolithotrophs and the second ones by chemoheterotrophs. Then, we collected data of the microbial communities of hot springs around the world in an effort to comprehend the roles of pH and temperature as shaping factors. Interestingly, there was a covariation between both parameters and the phylogenetic distance between communities; however, neither of them could explain much of the microbial profile in an ordination model. Moreover, there was no correlation between alpha diversity and these parameters. Therefore, the microbial communities’ profile seemed to have complex shaping factors beyond pH and temperature. Lastly, we looked for taxa associated with different environmental conditions. Several such taxa were found. For example, Hydrogenobaculum was frequently present in acidic springs, as was the Sulfolobaceae family; on the other hand, Candidatus Hydrothermae phylum was strongly associated with circumneutral conditions. Interestingly, some singularities related to sites featuring certain taxa were also observed.

scholarly journals Association of miR-1251-5p/miR-6892-5p Expression With Clinicopathological Factors in Premenopausal Endometrial Cancer

2021 ◽  
Author(s):  
Jing Zhang ◽  
Bin Li ◽  
Yang Liu ◽  
Fei Wang ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Target Genes ◽  
Tumor Grading ◽  
Node Metastasis ◽  
Predict Mirna Target ◽  
Next Generation Sequencing Ngs ◽  
The Relationship ◽  
Generation Sequencing ◽  
Menarche Age ◽  
Selection Of

Abstract Background This study is aim to profile the differentially expressed microRNAs (DEMs) of premenopausal endometrial cancer (EC), identify their target genes and understand their roles in carcinogenesis. Methods Next-generation sequencing (NGS) was performed on 3 premenopausal EC and 3 premenopausal normal endometrial tissues. Selection of candidate miRNAs and subsequent validation were performed by qRT-PCR on 20 premenopausal EC, 30 premenopausal normal endometrial and 40 postmenopausal EC samples. The relationship between DEMs and clinical characteristics was analyzed. Moreover, bioinformatic software programs and databases were applied to predict miRNA target genes, molecular functions, and signaling pathways. Results 136 upregulated and 131 downregulated DEMs were identified. The expression of miR-1251-5p was highly upregulated in premenopausal EC samples compared with premenopausal normal endometrial samples and significantly downregulated compared with postmenopausal EC samples. The expression of miR-6892-5p was highly upregulated in premenopausal EC samples compared with premenopausal normal endometrial samples and postmenopausal EC samples. In the premenopausal EC group, miR-1251-5p expression was closely correlated with menarche age, number of pregnancies, tumor grading, myometrial infiltration and lymph node metastasis; miR-6892-5p expression was closely correlated with BMI, hypertension, tumor grading, and metastasis. Conclusions miR-1251-5p and miR-6892-5p may play important roles in tumorigenesis progression of premenopausal EC.

scholarly journals Transgenerational inheritance of shuffled symbiont communities in the coral Montipora digitata

2018 ◽  
Author(s):  
Kate M. Quigley ◽  
Bette L. Willis ◽  
Carly D. Kenkel
Keyword(s):  
Thermal Tolerance ◽  
Coral Species ◽  
Early Life History ◽  
Amplicon Sequencing ◽  
Life History Stages ◽  
Genetic Mechanisms ◽  
Symbiont Transmission ◽  
Plastic Changes ◽  
Cell Densities ◽  
Symbiont Shuffling

AbstractAdult organisms may “prime” their offspring for environmental change through a number of genetic and non-genetic mechanisms, termed parental effects. Some coral species can alter their thermal tolerance by shuffling the proportions of Symbiodinium types within their endosymbiotic communities, but it is unclear if this plasticity can be transferred to offspring in corals that have maternal symbiont transmission. We evaluated symbiont community composition in tagged colonies of Montipora digitata from Orpheus Island, Australia, over two successive annual spawning seasons, the second of which overlapped with the 2016 mass coral bleaching event on the Great Barrier Reef. We applied amplicon sequencing of the ITS2 locus to samples of four families (parent colonies and 10-12 eggs per family) to characterize their potential for symbiont shuffling and to determine if shuffled abundances were preserved in gametes. Symbiont cell densities and photochemical efficiencies of the symbionts’ photosystem II differed significantly among adults in 2016, suggesting differential responses to increased temperatures. Although abundances of the dominant symbiont haplotype, a representative of clade C15, did not differ among families or over time, low-abundance (“background”) ITS2 types differed more among years (2015 vs. 2016) than between life stages (parent vs. offspring). Results indicate that background symbiont shuffling can occur in a canonically ‘stable’ symbiosis, and that such plastic changes to the symbiont community are heritable. To our knowledge, this is the first evidence that shuffled Symbiodinium communities can be inherited by early life-history stages and supports the hypothesis that plastic changes in microbial communities may serve as a mechanism of rapid coral acclimation to changing environmental conditions.

Sign in / Sign up

Export Citation Format

Share Document