scholarly journals Combined bio-chemical fertilizers ameliorate agro-biochemical attributes of black cumin (Nigella sativa L.)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Samira Moradzadeh ◽  
Sina Siavash Moghaddam ◽  
Amir Rahimi ◽  
Latifeh Pourakbar ◽  
R. Z. Sayyed
Keyword(s):  
Essential Oil ◽  
Azotobacter Vinelandii ◽  
Nigella Sativa ◽  
Block Design ◽  
Radical Scavenging ◽  
Control Treatment ◽  
Total Phenolic ◽  
Chemical Fertilizers ◽  
Branch Diameter ◽  
Black Cumin

AbstractNigella sativa L. is a medicinal plant with extensive, nutritional, pharmaceutical, and health applications. Nowadays, reducing the application of chemical fertilizers (synthetic fertilizers) is one of the main goals of sustainable agriculture to allow the production of safe crops. Therefore, the combined effect of urea and biofertilizers was studied on the quantitative and qualitative traits of N. sativa L. in a randomized complete block design with 10 treatments and three replications. The treatments included control (no fertilization), U (100% chemical fertilizer as urea at 53.3 kg ha−1, Nb (Biofertilizer, Azotobacter vinelandii), Pb (Biofertilizer, Pantoea agglomerans and Pseudomonas putida), Kb (Biofertilizer, Bacillus spp.), NPKb (NPK, biofertilizer), Nb + 50% U, Pb + 50% U, Kb + 50%U and NPKb + 50% U. The NPK(b) + U50% was related to the highest quantity of plant height, branch diameter, capsule (follicle) number per plant, auxiliary branches, seed yield per plant, thousand-seed weight, essential oil content, total phenolic compounds, flavonoid content, DPPH free radical scavenging, nitric oxide (NO) radical scavenging, superoxide radical scavenging, chain-breaking activity, phosphorus content, and potassium content, along with U for the highest biological yield and (Pb) + U50% for the highest essential oil percentage which is close to (NPKb) + U50%. The lowest value was observed in all traits related to the control treatment except for branch diameter that was related to (NPKb). Hence, the application of (NPKb) + U50% as bio-chemical fertilizers improved N. sativa L. Traits, so it can be recommended.

scholarly journals Radical scavenging and antimicrobial activity of essential oil and extracts of Echinophora sibthorpiana Guss. from Macedonia

2014 ◽  
Vol 66 (1) ◽  
pp. 401-413 ◽  
Author(s):  
Ksenija Mileski ◽  
Ana Dzamic ◽  
Ana Ciric ◽  
Slavica Grujic ◽  
M. Ristic ◽  
...  
Keyword(s):  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Ethanol Extract ◽  
Antimicrobial Effect ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Aerial Parts ◽  
Positive Controls

This study was undertaken to determine the antioxidant and antimicrobial effect of essential oil and extracts of Echinophora sibthorpiana Guss. (fam. Apiaceae) collected in Macedonia. The chemical composition of E. sibthorpiana essential oil was characterized by the presence of methyl eugenol (60.40%), p-cymene (11.18%) and ?-phellandrene (10.23%). The free radical scavenging activity of extracts and essential oil was evaluated by DPPH and ABTS assays. The aqueous extract of aerial parts exhibited the strongest scavenging activity (IC50=1.67 mg/ml); results of the ABTS test showed that the most effective was the ethanol extract of aerial parts (1.11 mg vit. C/g). The essential oil showed stronger antioxidant activity compared to hydroxyanisole, ascorbic acid and quercetin that were used in the DPPH and ABTS tests, respectively. The total phenolic and flavonoid concentrations in the extracts ranged between 38.65-60.72 mg GA/g, and 3.15-19.00 mg Qu/g, respectively. The antimicrobial properties of the extracts and essential oil were investigated using a micro-well dilution technique against human pathogenic strains. The results were comparable with the effects of the positive controls, streptomycin and fluconazole. These findings indicate that E. sibthorpiana extracts and oil can be used in preventive treatments and as an alternative for synthetic preservatives.

scholarly journals Antioxidant Activities and Reduced Amyloid-β Toxicity of 7-Hydroxycalamenene Isolated from the Essential Oil of Zelkova serrata Heartwood

2016 ◽  
Vol 11 (9) ◽  
pp. 1934578X1601100
Author(s):  
Pei-Ling Yen ◽  
Sen-Sung Cheng ◽  
Chia-Cheng Wei ◽  
Huan-You Lin ◽  
Vivian Hsiu-Chuan Liao ◽  
...  
Keyword(s):  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Amyloid Β ◽  
Total Phenolic ◽  
C Elegans ◽  
In Vivo Assays ◽  
Zelkova Serrata

The in vitro and in vivo antioxidant activities and its potential to protect against amyloid-β toxicity of essential oils from Zelkova serrata (Thunb.) Makino were investigated in the model organism Caenorhabditis elegans. The results revealed that the essential oil of Z. serrata heartwood exhibited great radical scavenging activities and high total phenolic content. In vivo assays showed significant inhibition of oxidative damage in wild-type C. elegans under juglone-induced oxidative stress and heat shock. Based on results from both in vitro and in vivo assays, the major compound in essential oil of heartwood, (-)-(1 S, 4 S)-7-hydroxycalamenene (1 S, 4 S-7HC), may contribute significantly to the observed antioxidant activity. Further evidence showed that 1 S, 4 S-7HC significantly delayed the paralysis phenotype in amyloid beta-expressing transgenic C. elegans. These findings suggest that 1 S, 4 S-7HC from the essential oil of Z. serrata heartwood has potential as a source for antioxidant or Alzheimer's disease treatment.

scholarly journals Chemical Composition and Antioxidant Activity of the Essential Oil and Fatty Acids of the Flowers of Rhanterium Adpressum

2013 ◽  
Vol 8 (8) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Chahrazed Hamia ◽  
Nadhir Gourine ◽  
Hadjer Boussoussa ◽  
Mokhtar Saidi ◽  
Emile M. Gaydou ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Lipid Extract ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Oil Extract

The essential oil obtained by hydrodistillation of the flowers of Rhanterium adpressum Coss. & Durieu was analyzed using GC and GC-MS. The essential oil was very rich in monoterpene compounds. The major components identified were the monoterpene hydrocarbons: camphene (21.8%), myrcene (19.3%) and α-pinene (17.4%). Other compounds, including limonene, β-pinene and terpinol-4-ol, were present in low content (4–6%). The composition of the fatty acids in the lipid extract obtained from the flowers was also investigated by GC and GC-MS. The main fatty acids identified were palmitic (47.4%), oleic (12.9%) and stearic acids (10.6%). The total phenolic contents and the antioxidant activities were also evaluated for both extracts. The total phenolic contents were determined using the Folin-Ciocalteu reagent and the antioxidant activities were measured using three different assays: DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity, FRAP (ferric reducing antioxidant potential) and a molybdenum assay. As a result of these tests, the lipid extract exhibited the highest antioxidant activities in comparison with the essential oil extract.

scholarly journals ANTIOXIDANT ACTIVITY, PHYTOCHEMICAL ANALYSIS AND TOTAL POLYPHENOLICS CONTENT OF ESSENTIAL OIL, METHANOL EXTRACT AND METHANOL FRACTIONS FROM COMMELINA NUDIFLORA

2018 ◽  
Vol 10 (8) ◽  
pp. 36 ◽  
Author(s):  
Muhammad Dawood Shah ◽  
Mohammad Iqbal
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Ethyl Acetate ◽  
Palmitic Acid ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Hexadecanoic Acid ◽  
Methanol Extracts

Objective: In the present study, the essential oil, methanol extract, and methanol fractions (n-hexane, chloroform, ethyl acetate, and n-butanol) obtained from Commelina nudiflora were investigated for the free radical scavenging effects and phytochemical analysis.Methods: The antioxidative effect of the essential oil, methanol extracts and methanol fractions were evaluated using 2, 2 diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Total phenolic and flavonoid contents were determined using Folin-Ciocalteau and aluminium chloride reagents respectively. The phytochemical analyses of the essential oil, methanol extracts and methanol fractions were performed by gas chromatography and mass spectrometry (GCMS). Results: The antioxidant, total phenolic and total flavonoid contents of butanol, ethyl acetate and chloroform fractions were higher followed by methanol extract, hexane fraction and essential oil. Phytochemical analysis indicated the presence of alkaloid, saponin, steroid, phytosterols, triterpenoids and tannins etc. The identified bioactive constituents of essential oil, methanol extract and methanol fractions of C. nudiflora were indole, 2-methoxy-4-vinylphenol, 2-pentadecanone, 6,10,14-trimethyl, phenol, benzyl alcohol, eugenol, phenol, 2, 4-bis (1,1-dimethylethyl), hexadecanoic acid, ethyl ester (palmitic acid ester), n-hexadecanoic acid (palmitic acid), 9, 12-octadecadienoic acid, (linoleic acid) and phytol. All identified bioactive compounds and their derivatives were generally reported with antimicrobial, antioxidant, anti-inflammatory and antitumor properties.Conclusion: The obtained data suggest that the essential oil, methanol extract and methanol fractions of C. nudiflora possess remarkable antioxidant activities and vital phytochemicals. Thus the plant can be a utilized as a potential source of nutraceutical with antioxidant activity.

scholarly journals Composition, In Vitro Antioxidant and Antimicrobial Activities of Essential Oil and Oleoresins Obtained from Black Cumin Seeds (Nigella sativaL.)

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Sunita Singh ◽  
S. S. Das ◽  
G. Singh ◽  
Carola Schuff ◽  
Marina P. de Lampasona ◽  
...  
Keyword(s):  
Essential Oil ◽  
Ethyl Acetate ◽  
Linseed Oil ◽  
Radical Scavenging ◽  
Thiobarbituric Acid ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Black Cumin

Gas chromatography-mass spectrometry (GC-MS) analysis revealed the major components in black cumin essential oils which were thymoquinone (37.6%) followed by p-cymene (31.2%),α-thujene (5.6%), thymohydroquinone (3.4%), and longifolene (2.0%), whereas the oleoresins extracted in different solvents contain linoleic acid as a major component. The antioxidant activity of essential oil and oleoresins was evaluated against linseed oil system at 200 ppm concentration by peroxide value, thiobarbituric acid value, ferric thiocyanate, ferrous ion chelating activity, and 1,1-diphenyl-2-picrylhydrazyl radical scavenging methods. The essential oil and ethyl acetate oleoresin were found to be better than synthetic antioxidants. The total phenol contents (gallic acid equivalents, mg GAE per g) in black cumin essential oil, ethyl acetate, ethanol, and n-hexane oleoresins were calculated as11.47±0.05,10.88±0.9,9.68±0.06, and8.33±0.01, respectively, by Folin-Ciocalteau method. The essential oil showed up to 90% zone inhibition againstFusarium moniliformein inverted petri plate method. Using agar well diffusion method for evaluating antibacterial activity, the essential oil was found to be highly effective against Gram-positive bacteria.

scholarly journals Nutrient management on the growth and yield of black cumin (Nigella sativa L.)

2018 ◽  
Vol 43 (2) ◽  
pp. 205-210
Author(s):  
MN Yousuf ◽  
AJMS Karim ◽  
ARM Solaiman ◽  
MS Islam ◽  
M Zakaria
Keyword(s):  
Seed Yield ◽  
Nutrient Management ◽  
Nigella Sativa ◽  
Block Design ◽  
Winter Season ◽  
Research Field ◽  
Growth And Yield ◽  
Black Cumin ◽  
Randomized Complete Block Design ◽  
Positive Effect

A field experiment was carried out at the research field of Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur, during rabi (winter) season of 2016-17 to evaluate the effects of fertilizer treatments on black cumin (BARI Kalozira-1). The field belongs to Shallow red-brown terrace soil of Salna series under AEZ-28 (Madhupur Tract). The experiment was laid out in randomized complete block design (RCBD) with three replications. Results showed that the application of nutrient elements had positive effect on plant height, branches per plant, capsule setting, umbels per plant, capsules per plant, capsule size, seeds per capsule, 1000-seed weight and seed yield of black cumin. The highest seed yield (1277 kg ha-1) was obtained from 75% RDCF + 25% cowdung-N treatment followed by 100% RDCF (N80P45K50S20Zn5B2 kg ha-1) and the lowest seed yield (420 kg ha-1) was recorded with 50% RDCF. Thus, the IPNS treatment 75% RDCF + 25% cowdung-N appeared to be the best suitable package for black cumin cultivation in this location.Bangladesh J. Agril. Res. 43(2): 205-210, June 2018

scholarly journals Interactive effects of rice bran compost and chemical fertilizers on macronutrients, oil and protein content in sunflower (Helianthus annuus L.)

2021 ◽  
Vol 10 (2) ◽  
pp. 91-99
Author(s):  
M Alauddin ◽  
GM Mohsin ◽  
AHMZ Ali ◽  
MK Rahman
Keyword(s):  
Protein Content ◽  
Rice Bran ◽  
Seed Quality ◽  
Block Design ◽  
Interactive Effects ◽  
Control Treatment ◽  
Chemical Fertilizers ◽  
Uptake Pattern ◽  
The Impact

A field experiment was conducted at the research farm of Charfasson Govt. College, Bhola, Bangladesh in rabi season in 2015-2016 to evaluate the impact of conjunctive use of chemical fertilizers with rice bran on concentration, uptake and seed quality of sunflower cv. BARI-2 (Keroni-2). The experiment was laid out in the randomized complete block design (RCBD) having sixteen treatments with three replications. The size of the plots were 3 m x 2 m. Treatments were T1 Control (- RB and -NPK), T2: 2.5 t RB ha-1, T3: 5.0 t RB ha-1,T4: 7.5 t RB ha-1, T5: N40P30K50 kg ha-1,T6: N80P60K100 kg ha-1, T7: N120P90K150 kg ha-1, T8: 2.5 t RB ha-1 + N40P30K50 kg ha-1, T9: 2.5 t RB ha-1 + N80P60K100 kg ha-1, T10: 2.5 t RB ha-1 + N120P90K150 kg ha-1, T11: 5.0 t RB ha-1 + N40P30K50 kg ha-1, T12: 5.0 t RB ha-1 + N80P60K100 kg ha-1, T13: 5.0 t RB ha-1 + N120P90K150 kg ha-1, T14: 7.5 t RB ha-1 + N40P30K50 kg ha-1, T15: 7.5 t RB ha-1 + N80P60K100 kg ha-1, T16: 7.5 t RB ha-1 + N120P90K150 kg ha-1. Results showed that the concentration, uptake and quality of seeds (oil and protein) of the crop increased with increasing rate of the amendments significantly (P<0.05) over the control and the variation between the treatments were also significant irrespective of the sources of amendments in most of the cases. Generally, combination of the treatments showed better performance than their individual application. Maximum values of NPKS concentration (%) in different organs of sunflower were 1.22, 0.35, 1.90, 0.18 for stem; 1.17, 0.35, 2.41, 0.16 for root; 3.98, 0.43, 4.28, 0.24 for leaf, 1.04, 0.65, 3.00, 0.22 for petiole; 2.16, 0.58, 2.21, 0.26 for inflorescence and 5.24, 0.83, 1.60, 0.47 for seed measured in treatments 5.0 t RB ha-1 + N120P90K150 kg ha-1 and 7.5 t RB ha-1 + N120P90K150 kg ha-1 in most of the cases. However, their uptake pattern also followed the same trend as in concentration and the highest values were found in those treatments in most of the cases. Significantly (P<0.05%) the highest content of oil (51.1%) in seed was measured in the treatment 5.0 t RB ha-1 + N120P90K150 kg ha-1 and protein (33.9%) was found in the treatment 5.0 t RB ha-1 +N80P60K100 kg ha-1. Their lowest values were found in control for oil and in 2.5 t RB ha-1 for protein, which was lower than control treatment. The overall findings of this study indicated that rice bran in combination with chemical fertilizers could be applied to achieve better concentration and uptake in different organs, oil and protein content in seeds of sunflower. Int. J. Agril. Res. Innov. Tech. 10(2): 91-99, December 2020

scholarly journals Essential oil and thymol of ajwain as effective antioxidant agents

1970 ◽  
pp. 141-146
Author(s):  
J Gaba ◽  
S Sharma ◽  
P Kaur ◽  
S Joshi
Keyword(s):  
Essential Oil ◽  
Radical Scavenging ◽  
Major Constituent ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Spectral Techniques ◽  
Antioxidant Agents ◽  
Effective Antioxidant ◽  
Total Antioxidant

Ajwain is an essential herbal plant with many health and medicinal values. Quantitative phytochemical analysis of ajwain was carried out to determine total phenolic compounds, flavonoids, saponins and tannin content. The phytochemical contents were in the order saponins > tannins > flavonoids > phenolics. Essential oil was extracted and chromatographed for the major constituent thymol which was further characterized with spectral techniques. Further, role of essential oil of ajwain seeds and its major compound thymol for antioxidant activity in respect of total antioxidant capacity and free radical scavenging potential were worked out. Out of thymol and ajwain oil, former was found to be more effective antioxidant at all tested concentrations. Inhibition concentrations (IC50) of oil, thymol and ascorbic acid were found to be 1.87, 1.46 and 0.01 mg mL-1, respectively. Results suggest that ajwain and thymol can be used as natural substitutes to synthetic antioxidants in preservation of various food products.

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