scholarly journals Use of Lactobacillus plantarum (strains 22F and 25F) and Pediococcus acidilactici (strain 72N) as replacements for antibiotic-growth promotants in pigs

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pawiya Pupa ◽  
Prasert Apiwatsiri ◽  
Wandee Sirichokchatchawan ◽  
Nopadon Pirarat ◽  
Tanawong Maison ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Average Daily Gain ◽  
Intestinal Morphology ◽  
Finishing Pigs ◽  
Pediococcus Acidilactici ◽  
Feed Conversion ◽  
Neonatal Pigs ◽  
Peptone Water ◽  
Antibiotic Group ◽  
Growth Promotants

AbstractThe lactic acid bacteria (LAB) Lactobacillus plantarum (strains 22F and 25F) and Pediococcus acidilactici (strain 72N) have appeared promising as replacements for antibiotics in in vitro studies. Microencapsulation, especially by the spray-drying method, has been used to preserve their numbers and characteristics during storage and digestion. This study compared the efficacy of these strains and their microencapsulated form with antibiotic usage on growth performance, faecal microbial counts, and intestinal morphology in nursing-finishing pigs. A total of 240 healthy neonatal pigs were treated on days 0, 3, 6, 9, and 12 after cross-fostering. Sterile peptone water was delivered orally to the control and antibiotic groups. Spray-dried Lactobacillus plantarum strain 22F stored for 6-months was administered to piglets in the spraydry group. Three ml of each the three fresh strains (109 CFU/mL) were orally administered to piglets in each group. All pigs received the basal diets, but these were supplemented with routine antibiotic for the antibiotic group. Pigs in all the probiotic supplemented groups exhibited a better average daily gain and feed conversion ratio than those of the controls in the nursery and grower phases. Probiotic supplementation increased viable lactobacilli and decreased enterobacterial counts. Antibiotic additives reduced both enterobacterial and lactobacilli counts. Villous height and villous height:crypt depth ratio were greater in probiotic and antibiotic supplemented pigs comparing to the controls, especially in the jejunum. The results demonstrated the feasibility of using these strains as a substitute for antibiotics and the practicality of the microencapsulation protocol for use in swine farms.

Use of Lactobacillus plantarum (strains 22F and 25F) and Pediococcus acidilactici (strain 72N) as replacements for antibiotic-growth promotants in pigs

2021 ◽  
Author(s):  
Pawiya Pupa ◽  
Prasert Apiwatsiri ◽  
Wandee Sirichokchatchawan ◽  
Noppadon Pirarat ◽  
Tanawong Maison ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Average Daily Gain ◽  
Intestinal Morphology ◽  
Pediococcus Acidilactici ◽  
Feed Conversion ◽  
Peptone Water ◽  
Antibiotic Group ◽  
Growth Promotants ◽  
Spray Dried

Abstract Lactobacillus plantarum (strains 22F and 25F) and Pediococcus acidilactici (strain 72N) have appeared promising in in vitro studies. Microencapsulation, especially by the spray-drying method, has been used to preserve their numbers and characteristics during storage and digestion. This study compared the efficacy of these LAB strains and their microencapsulated form with antibiotic usage on growth performance, faecal microbial counts, and intestinal morphology in nursing-finishing pigs. A total of 240 healthy neonatal pigs were treated on days 0, 3, 6, 9, and 12 after cross-fostering. Sterile peptone water was delivered orally to the control and antibiotic groups. Six-month storage spray-dried Lactobacillus plantarum strain 22F was administered to piglets in the spraydry group. The three fresh strains of LAB (109 CFU/mL) were orally administered to piglets in each group. All pigs received the basal diets, but these were supplemented with routine antibiotic for the antibiotic group. Pigs in the probiotic supplemented groups (spray-dried and three fresh strains of LAB) exhibited a better average daily gain and feed conversion ratio than those of the controls in the nursery and grower phases. Probiotic supplementation increased viable lactobacilli and decreased enterobacterial counts. Antibiotic additives reduced both enterobacterial and lactobacilli counts. Villous height and villous height:crypt depth ratio were greater in probiotic and antibiotic supplemented pigs comparing to the controls, especially in the jejunum. The results demonstrated the feasibility of using these strains as a substitute for antibiotics and the practicality of the microencapsulation protocol for use in swine farms.

scholarly journals Propolis supplementation affects performance, intestinal morphology, and bacterial population of broiler chickens

2021 ◽  
Vol 51 (4) ◽  
pp. 477-487
Author(s):  
S. Sadarman ◽  
E. Erwan ◽  
A. Irawan ◽  
M.M. Sholikin ◽  
R. Solfaine ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Bacterial Population ◽  
Mixed Model ◽  
Meta Analysis ◽  
Digestive Enzyme ◽  
Average Daily Gain ◽  
Intestinal Morphology ◽  
Detectable Effect ◽  
Optimum Level ◽  
Feed Conversion

A meta-analysis was conducted to examine the effect of supplementing the diet of broiler chickens with propolis on growth, bacterial population of the intestine, antiviral serum concentration, intestinal morphology, and digestive enzyme activities in broiler chickens. Forty peer-reviewed articles that had been published between 2003 and 2019 were identified using the PRISMA protocol and included in the study. Data were analysed with mixed model methodology, in which the studies were considered random effects, whereas the level of supplemental propolis was considered a fixed effect. Responses to propolis supplementation in bodyweight (BW) and average daily gain (ADG) were quadratic, but average daily feed intake (ADFI) was not affected. Propolis supplementation improved feed conversion ratio (FCR) significantly as a linear function of the level of supplement. The optimum level of supplementation was between 256 and 262 mg/kg feed and produced maximum ADG and final BW. There was a tendency for mortality to decrease because of propolis supplementation. Propolis had no detectable effect on serum antiviral concentration, intestinal bacterial population or intestinal morphology. Among digestive enzymes, only sucrase increased linearly as propolis was increased. Thus, supplementation with propolis increased the growth performance of broiler chickens positively and the effect was dose dependent. This may have been partly because of an improvement in sucrase activity and other factors related to the nutritional content of propolis. Future study to evaluate specific bioactive compounds of propolis is therefore warranted.

scholarly journals Effect of a Buttiauxella phytase on production performance in growing/finishing pigs fed a European-type diet without inclusion of inorganic phosphorus

2017 ◽  
Vol 5 ◽  
Author(s):  
Yueming Dersjant-Li ◽  
Katharina Schuh ◽  
Alexandra L. Wealleans ◽  
Ajay Awati ◽  
Georg Dusel
Keyword(s):  
Energy Content ◽  
Dose Range ◽  
Average Daily Gain ◽  
Inorganic Phosphorus ◽  
Negative Control ◽  
Production Performance ◽  
Finishing Pigs ◽  
Feed Conversion ◽  
Inorganic P ◽  
Dietary Treatments

SummaryThe effect of a Buttiauxella phytase, in a dose range of 0-1000 FTU/kg in feed, on production performance in growing/finishing pigs fed European type wheat, corn, barley and SBM based diet was determined. Five dietary treatments were tested including a positive control (PC), a negative control (NC) without inorganic P and with reduced Ca (–0.12%) and metabolisable energy content (–0.14 MJ ME/kg) or NC supplemented with Buttiauxella phytase (Axtra® PHY, Danisco Animal Nutrition, DuPont Industrial Biosciences, Marlborough, UK) at 250, 500 or 1000 FTU/kg respectively. A total of 100 pigs (mean initial BW of 30 kg, Topig x Pi, 10 weeks of age) were used, with 20 replicates per treatment (50% gilts and 50% barrows). Pigs were individually housed in pens and randomly allocated on the basis of body weight (BW) and gender to the five treatments. Feed, in mash form, and water were offered ad libitum. Basal diets had an intrinsic phytase level of approximately 300 and 440 FTU/kg in NC grower and NC finisher diets respectively. Mean average daily gain (ADG), average daily feed intake (ADFI) and feed conversion ratio (FCR) were calculated for the feeding phases of 30–85 kg (grower), 85–125 kg (finisher) on trial and for overall period. During the grower phase, increasing phytase dose resulted in a linear increase in ADG. Phytase inclusion at 250, 500 and 1000 FTU/kg improved ADG by 3.5, 7.2 and 8.1% respectively compared to NC and by 0.8, 4.5 and 5.3% respectively compared to those fed the PC. Performance in the finisher phase, overall period and slaughter parameters were not different between dietary treatments. The data showed that it was beneficial to increase phytase dose up to 1000 FTU/kg in grower pigs up to 85 kg BW. In the finisher phase, in pigs with BW above 85 kg, 500 FTU/kg was sufficient to replace inorganic P and maintain performance and carcass characteristics comparable to pigs fed the PC.

scholarly journals Effects of microbial phytase supplementation of a barley-soybean meal diet on the performance and bone mineralization of growing-finishing pigs

1994 ◽  
Vol 3 (5) ◽  
pp. 439-448
Author(s):  
Eija Helander
Keyword(s):  
Bone Mineralization ◽  
Control Diet ◽  
Average Daily Gain ◽  
Physical Parameters ◽  
Finishing Pigs ◽  
Feed Conversion ◽  
Inorganic P ◽  
Microbial Phytase ◽  
Daily Gain ◽  
Better Than

The study evaluated the effect of a phytase supplement, produced by Aspergillus niger, on the performance of 72 growing-finishing pigs (28-101 kg). Chemical and physical parameters of the tibia and fibula bones were measured. The control diet (diet 1) was formulated to be adequate for pigs with respect to all nutrients, and diets 2 and 3 with respect to all other nutrients except total phosphorus (P). The P source of the control diet was dicalciumphosphate (dihydrate), while no inorganic P was added to diets 2 and 3. Diet 3 was supplemented with phytase, 720 U/kg feed. The average daily gain (ADG) and the feed conversion ratio (FCR) of the control pigs were significantly better than those of the groups without inorganic P. Phytase improved the ADG (p

scholarly journals Algae-Derived Polysaccharides Promote Growth Performance by Improving Antioxidant Capacity and Intestinal Barrier Function in Broiler Chickens

2020 ◽  
Vol 7 ◽  
Author(s):  
Wen-Chao Liu ◽  
Yan Guo ◽  
Zhi-Hui Zhao ◽  
Rajesh Jha ◽  
Balamuralikrishnan Balasubramanian
Keyword(s):  
Antioxidant Capacity ◽  
Growth Performance ◽  
Average Daily Gain ◽  
Intestinal Morphology ◽  
Intestinal Barrier Function ◽  
Control Group ◽  
Gut Health ◽  
Feed Conversion ◽  
Villus Height ◽  
Final Body Weight

This study aimed to determine the efficacy of dietary algae-derived polysaccharides (ADPs) from Enteromorpha on growth performance, intestinal morphology, intestinal permeability, and antioxidant capacity in serum, liver, and intestinal mucosa of broilers. Three hundred and ninety six day-old male chicks were randomly assigned to six dietary treatments containing 0 (Control), 1,000, 2,500, 4,000, 5,500, and 7,000 mg ADP/kg basal diet in a 35 day feeding trial. During day 1–21, compared with the control group, dietary 1,000–7,000 mg/kg ADP supplementation improved the average daily gain (ADG) and feed conversion ratio (p < 0.05). Overall (day 1–35), dietary inclusion of 1,000 mg/kg ADP increased the final body weight and ADG (p < 0.05). Besides, on day 21, dietary 2,500 mg/kg ADP supplementation increased the serum catalase (CAT) and liver total superoxide dismutase (T-SOD) activities (p < 0.05), whereas dietary 1,000–5,500 mg/kg ADP supplementation decreased malondialdehyde (MDA) contents in serum and liver (p < 0.05). On day 35, supplementation of 1,000 mg/kg ADP increased the serum glutathione peroxidase and CAT activities and liver T-SOD activities (p < 0.05). It decreased the MDA level of serum and liver (p < 0.05). Also, dietary 2,500 mg/kg ADP increased the villus height of jejunum and ileum on day 21 (p < 0.05), and dietary 4,000 mg/kg ADP increased the villus height of duodenum and ileum on day 35 (p < 0.05). On day 21, dietary 4,000 mg/kg ADP increased the CAT activities of the duodenum and T-SOD activities of jejunum and ileum and decreased the MDA contents in the duodenum, jejunum, and ileum (p < 0.05). On day 35, dietary inclusion of 1,000–7,000 mg/kg ADP reduced MDA contents of duodenum and jejunum (p < 0.05). Furthermore, dietary inclusion of ADP at 1,000–7,000 mg/kg decreased serum DAO activities at day 21 and day 35 (p < 0.05), and the serum D-lactic acid concentration was reduced by dietary supplementation of 1,000, 2,500, and 7,000 mg/kg ADP on day 21. In conclusion, dietary ADP exerted beneficial effects on growth performance, antioxidant capacity, and gut health in broilers; based on the studied parameters, the appropriate recommended dose is 1,000–4,000 mg/kg. These findings provided new insights into the potential application of ADP as natural growth promoters in broilers.

scholarly journals Meat quality and performance of pigs fed diets with fish silage meal

2017 ◽  
Vol 52 (10) ◽  
pp. 905-913
Author(s):  
Rayssa Santos Cândido ◽  
Pedro Henrique Watanabe ◽  
Paula Joyce Delmiro de Oliveira ◽  
Alysson Lira Angelim ◽  
André de Freitas Siqueira ◽  
...  
Keyword(s):  
Meat Quality ◽  
Average Daily Gain ◽  
Nutrient Digestibility ◽  
Metabolizable Energy ◽  
Economic Viability ◽  
Finishing Pigs ◽  
Feed Conversion ◽  
Inclusion Level ◽  
And Performance ◽  
Daily Gain

Abstract: The objective of this work was to determine the nutrient digestibility and metabolizable energy (ME) of fish silage, as well as to evaluate the effect of the dietary inclusion of fish silage meal (FSM) in diets on the performance, carcass characteristics, meat quality, sensory analysis of meat and mortadella, and economic viability of growing and finishing pigs. In the digestibility assay, 16 barrows (33.20±4.93 kg) received diets with and without FSM. The fish silage had 39.01% crude protein and 4,032 kcal kg-1 ME. In the performance assay, 32 barrows (26.00±1.68 kg) were fed diets containing different inclusion levels of FSM (0, 25, 50, and 75%). FSM, obtained from the mixture (1:1) of fish silage with corn, showed a quadratic effect on average daily gain, and the best result was obtained with the inclusion level of 25.83%. The results for feed conversion and economic viability indicate that up to 25% FSM, corresponding to 5.87% of fish silage based on dry matter, can be used in the pig growing and finishing phases.

Supplementing feed with Pediococcus acidilactici reduces Campylobacter load in finishing pigs

2020 ◽  
Vol 187 (6) ◽  
pp. e45-e45
Author(s):  
Ángela Morales-Partera ◽  
Fernando Cardoso Toset ◽  
Inmaculada Luque ◽  
Alfonso Maldonado ◽  
Carmen Tarradas ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Control Diet ◽  
Average Daily Gain ◽  
Treated Group ◽  
Control Group ◽  
Finishing Pigs ◽  
Pediococcus Acidilactici ◽  
Performance Parameters ◽  
Free Range ◽  
Group Control

BackgroundPigs are asymptomatic carriers of foodborne bacteria, such as Salmonella enterica and Campylobacter species, which can pose a risk to human health. New strategies to control bacteria burden before reaching the slaughterhouse are necessary. This study evaluated the effect of Pediococcus acidilactici on performance parameters and on the burden of foodborne pathogens, that have subsequent implications on food quality and safety, in free-range finishing pigs at the slaughterhouse.MethodsPigs were randomly allocated and blocked by weight into control group (control diet) and treated group (control diet supplemented with P acidilactici) 31 days before slaughter. Weight and average daily gain were recorded and changes in faecal microbiota were determined at the beginning and at the end of the study.ResultsNo changes were observed in performance parameters. No statistically significant differences were observed when comparing between treated and control animals at the beginning or at the end of the study. However, a significant decrease was detected in the counts of Campylobacter species in treated animals between day 0 and day 31 (4.86–3.40 log colony-forming units/g; P=0.002).ConclusionThis study indicates that supplementation with P acidilactici represents a useful approach to control Campylobacter species load in free-range finishing pigs before slaughter.

scholarly journals Effects of Weaning Age at 21 and 28 Days on Growth Performance, Intestinal Morphology and Redox Status in Piglets

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2169
Author(s):  
Dongxu Ming ◽  
Wenhui Wang ◽  
Caiyun Huang ◽  
Zijie Wang ◽  
Chenyu Shi ◽  
...  
Keyword(s):  
Growth Performance ◽  
Average Daily Gain ◽  
Experimental Period ◽  
Redox Status ◽  
Intestinal Morphology ◽  
Feed Conversion ◽  
Study Objective ◽  
Entire Experimental Period ◽  
Diarrhea Incidence ◽  
Better Than

The study objective was to assess effects of different weaning ages on growth performance, intestinal morphology and redox status in Duroc × Landrace × Large White piglets (n = 96) fed diets without antibiotic growth promoters. Piglets were selected from 24 litters based on similar body weight at 14 d of age. All piglets were allocated to two groups in a completely random design with six replicates and eight pigs per replicate (four barrows and four gilts), which were weaned at 21 (n = 48; BW = 6.87 ± 0.33 kg) and 28 (n = 48; BW = 8.49 ± 0.41 kg) days of age. After weaning, pigs were fed a corn–soybean meal-based diet. Average daily gain (ADG), average daily feed intake (ADFI), feed conversion (F:G), diarrhea incidence, gastrointestinal pH, intestinal morphology and redox status were determined. Pigs weaned at 28 d displayed increased ADG from d 8 to 14 (p < 0.01) compared with pigs weaned at 21 d. Pigs weaned at 28 d had a higher ADFI from d 0 to 7 (p < 0.01), d 8 to 14 (p < 0.01), d 15 to 28 (p < 0.05) and during the entire experimental period (p < 0.01) compared with pigs weaned at 21 d. Pigs weaned at 21 d had an improved F:G from d 15 to 28 (p < 0.05) compared with pigs weaned at 28 d. Pigs weaned at 28 d had decreased diarrhea incidence from d 8 to 14 (p < 0.01) and the entire experimental period (p < 0.01) compared with pigs weaned at 21 d. On d 28, the pH of the stomach contents in pigs weaned at 21 d was significantly higher compared with pigs weaned at 28 d (p < 0.01). On d 14, the morphology of the duodenum, jejunum and ileum in pigs weaned at 28 d was improved compared with pigs weaned at 21 d. During the experiment period, the antioxidant abilities of pigs weaned at 28 d of the heart, liver, kidney, intestinal and serum were better than pigs weaned at 21 d. In conclusion, intestinal morphology, pH of the stomach and antioxidant status of pigs weaned at 28 d were better than pigs weaned at 21 d. These factors supported better growth performance and decreased diarrhea incidence.

scholarly journals PSVI-18 The use of Spirulina in piglet’s diets: effects on growth performance, selected biochemical blood parameters and small intestine mucosa morphology and metabolomics profiles

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 469-470
Author(s):  
Cátia F Martins ◽  
Manolis Matzapetakis ◽  
David D Ribeiro ◽  
Mário Pinho ◽  
João Pedro Bengala Freire ◽  
...  
Keyword(s):  
Small Intestine ◽  
Plasma Cholesterol ◽  
Energy Levels ◽  
Average Daily Gain ◽  
Experimental Period ◽  
Intestinal Morphology ◽  
Control Group ◽  
Feed Conversion ◽  
Plasma Parameters ◽  
Metabolomics Data

Abstract Pig production relies on expensive imported protein feedstuffs namely soybean meal. It is very important to use less conventional feedstuffs such as microalgae. Spirulina has been used in pig feeds as an additive (inclusion levels below 1%). Recently, its use as a protein feedstuff has been proposed. In order to study the effect of Spirulina on growth, intestinal mucosa morphology microscopy, plasma profiles and small intestine and liver metabolomics (1H NMR) of weaned 12 kg piglets, an experiment with 40 male animals was carried out. Animals were divided into 4 groups of 10 animals (Control, 10% Spirulina, 10% Spirulina + Rovabio® and 10% Spirulina + Lysozyme). Piglets were fed ad libitum. All diets had the same protein and energy levels. Piglets were weighed weekly; experimental period was 4 weeks. Animals were sacrificed and blood, liver and small intestine sampled. Variance analysis was used to test diet effect on growth, intestinal morphology and plasma parameters. Metabolomics data were analysed using PCA and PLS-DA. For the same feed intake, the average daily gain was reduced by 14% and feed-conversion ratio increased by 10% in the three diets with Spirulina in comparison to Control. No significant differences (P &gt; 0.05) were found for microvilli height and width or crypt depth. Plasma cholesterol, total lipids and liver aminotransferases (ALT, AST) and alkaline phosphatase (ALP) concentrations were higher in animals fed on Spirulina diets. Metabolic profiles for the two tissues were similar for the four groups, although PLS-DA analysis showed a tendency for the three Spirulina diets to cluster together, separately from the control group. Betaine and glycerol-3-phosphocholine were the most discriminant metabolites. In conclusion, although Spirulina had no significant effects on small intestinal morphology and metabolomics profiles, its use for piglet feeding in incorporation levels of 10% leads to losses in growth performances.

In vitro characterisation of two Lactobacillus strains and evaluation of their suitability as probiotics for growing-finishing pigs

2019 ◽  
Vol 59 (8) ◽  
pp. 1537
Author(s):  
D. Y. Zhang ◽  
H. F. Ji ◽  
S. X. Wang ◽  
H. Liu ◽  
J. Wang ◽  
...  
Keyword(s):  
Lactobacillus Reuteri ◽  
Control Diet ◽  
Average Daily Gain ◽  
Growing Pigs ◽  
Control Group ◽  
Probiotic Properties ◽  
Finishing Pigs ◽  
Feed Conversion ◽  
Large White

In this study, we evaluated the probiotic properties of two strains Lactobacillus reuteri ZLR003 and Lactobacillus salivarius ZLS006. The two strains displayed tolerance of acid and heat, and demonstrated antimicrobial ability in vitro. Furthermore, their potential functions in vivo were also tested. A total of 120 crossbred (Landrace × Large White) growing pigs were divided into three groups: a control diet, the same diet supplemented with L. reuteri ZLR003 (2.0 × 109 cfu/kg of diet) or L. salivarius ZLS006 (3.50 × 109 cfu/kg of diet). The results showed that the average daily gain and feed conversion ratio were significantly improved in L. reuteri ZLR003- (1–5 weeks and 1–9 weeks) (P &lt; 0.05) and L. salivarius ZLS006-treated pigs (1–5 weeks, 6–9 weeks and 1–9 weeks) (P &lt; 0.05) compared with the control group. Dietary supplementation with L. salivarius ZLS006 increased the apparent digestibility of nitrogen at Week 9 (P &lt; 0.05). The faecal Lactobacillus populations increased at the end of experiment, and the Escherichia coli and Staphylococcus aureus in faeces decreased in the two Lactobacillus treatments compared with the control at Week 5 (P &lt; 0.05) and Week 9 (P &lt; 0.05), respectively. Furthermore, the total cholesterol, alanine transferase, aspartate transferase, blood urea nitrogen and haptoglobin levels in serum were significantly decreased following L. reuteri ZLR003 and L. salivarius ZLS006 treatments (P &lt; 0.05). In conclusion, these data suggest that the two Lactobacillus strains may be promising candidates for probiotic products in growing-finishing pigs.

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