scholarly journals PSVI-18 The use of Spirulina in piglet’s diets: effects on growth performance, selected biochemical blood parameters and small intestine mucosa morphology and metabolomics profiles

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 469-470
Author(s):  
Cátia F Martins ◽  
Manolis Matzapetakis ◽  
David D Ribeiro ◽  
Mário Pinho ◽  
João Pedro Bengala Freire ◽  
...  

Abstract Pig production relies on expensive imported protein feedstuffs namely soybean meal. It is very important to use less conventional feedstuffs such as microalgae. Spirulina has been used in pig feeds as an additive (inclusion levels below 1%). Recently, its use as a protein feedstuff has been proposed. In order to study the effect of Spirulina on growth, intestinal mucosa morphology microscopy, plasma profiles and small intestine and liver metabolomics (1H NMR) of weaned 12 kg piglets, an experiment with 40 male animals was carried out. Animals were divided into 4 groups of 10 animals (Control, 10% Spirulina, 10% Spirulina + Rovabio® and 10% Spirulina + Lysozyme). Piglets were fed ad libitum. All diets had the same protein and energy levels. Piglets were weighed weekly; experimental period was 4 weeks. Animals were sacrificed and blood, liver and small intestine sampled. Variance analysis was used to test diet effect on growth, intestinal morphology and plasma parameters. Metabolomics data were analysed using PCA and PLS-DA. For the same feed intake, the average daily gain was reduced by 14% and feed-conversion ratio increased by 10% in the three diets with Spirulina in comparison to Control. No significant differences (P > 0.05) were found for microvilli height and width or crypt depth. Plasma cholesterol, total lipids and liver aminotransferases (ALT, AST) and alkaline phosphatase (ALP) concentrations were higher in animals fed on Spirulina diets. Metabolic profiles for the two tissues were similar for the four groups, although PLS-DA analysis showed a tendency for the three Spirulina diets to cluster together, separately from the control group. Betaine and glycerol-3-phosphocholine were the most discriminant metabolites. In conclusion, although Spirulina had no significant effects on small intestinal morphology and metabolomics profiles, its use for piglet feeding in incorporation levels of 10% leads to losses in growth performances.

2016 ◽  
Vol 37 (4) ◽  
pp. 1947 ◽  
Author(s):  
Kelly Mazutti ◽  
Leandro Batista Costa ◽  
Lígia Valéria Nascimento ◽  
Tobias Fernandes Filho ◽  
Breno Castello Branco Beirão ◽  
...  

For the last several decades, antimicrobial compounds have been used as feed additives to promote piglet growth at weaning, through the prevention of subclinical and clinical disease. However, few studies have assessed the influence of these antibiotics on the immune response of nursery pigs, as well as the relation between performance, health, and immunity of animals that receive feed additives. Therefore, the present study aimed to evaluate the effects of colistin and tylosin when used as feed additives on the performance, incidence of diarrhea, and immune response of nursery pigs. In this study, 72 weaned pigs (average age, 28 days) were allotted into one of three treatment groups: a control group (feed with no antibiotics), tylosin group (feed containing 22 ppm tylosin), and colistin group (feed containing 20 ppm colistin). Weekly, during a five week period, the average daily feed intake, average daily gain, and feed conversion ratio of the pigs were evaluated. Stools were scored daily, in accordance with a fecal texture scale. Blood samples were collected on the day of housing (d0) and on d7, d21, d28, and d35 for immune cell phenotyping. The results of this study showed that piglets in both the colistin and tylosin groups exhibited a significantly higher average daily feed intake, resulting in a higher body weight at the end of the experimental period (d35) when compared with piglets from the control group. Colistin and tylosin also significantly reduced the incidence of diarrhea. Colistin and tylosin modulated the piglets’ immune responses, particularly on d28, by changing the percentage of circulating B lymphocytes, CD4+CD8+ T cells, and the CD4:CD8 ratio.


2011 ◽  
Vol 50 (No. 10) ◽  
pp. 459-465 ◽  
Author(s):  
P. Daněk ◽  
A. Paseka ◽  
J. Smola ◽  
J. Ondráček ◽  
R. Bečková ◽  
...  

The influence of supplementation of a lecithin emulsifier (0.1%) to the feed mixture for piglets after weaning was studied. 16 piglets in the control group (C – without lecithin) and 16 piglets in an experimental (E) group were monitored in three replications in a 28-day experimental period. We observed higher digestibility of monitored nutrients in the experimental group (crude fat: C = 66.28 ± 12.11, E = 69.75 ± 9.85%, crude protein: C = 78.72 ± 5.47, E = 82.17 ± 2.23%, crude fibre: C = 56.70 ± 12.85, E = 59.10 ± 12.04%, nitrogen free extract: C = 86.67 ± 3.32, E = 87.75 ± 3.00%, ash: C = 63.63 ± 11.59, E = 65.73 ± 10.35%). Statistically insignificantly higher daily average gain (C = 374.1 ± 107.0, E = 399.1 ± 104.7 g) of experimental groups and a lower feed conversion ratio (C = 2.285 ± 0.416, E = 1.768 ± 0.048 kg/kg) were found out. It was also confirmed in an additional field experiment (C = 279, E = 312 piglets from birth to 71 days of age) where average daily gain weight was 270 g in control and 290 g in experimental group. The influence of lecithin on the occurrence of haemolytic strains of Escherichia coli was not proved. This experiment confirmed a positive effect of the lecithin emulsifier supplement on the efficiency of piglets.  


Author(s):  
Wen-Chao Liu ◽  
Yan Guo ◽  
Zhi-Hui Zhao ◽  
Rajesh Jha ◽  
Balamuralikrishnan Balasubramanian

This study aimed to determine the efficacy of dietary algae-derived polysaccharides (ADPs) from Enteromorpha on growth performance, intestinal morphology, intestinal permeability, and antioxidant capacity in serum, liver, and intestinal mucosa of broilers. Three hundred and ninety six day-old male chicks were randomly assigned to six dietary treatments containing 0 (Control), 1,000, 2,500, 4,000, 5,500, and 7,000 mg ADP/kg basal diet in a 35 day feeding trial. During day 1–21, compared with the control group, dietary 1,000–7,000 mg/kg ADP supplementation improved the average daily gain (ADG) and feed conversion ratio (p < 0.05). Overall (day 1–35), dietary inclusion of 1,000 mg/kg ADP increased the final body weight and ADG (p < 0.05). Besides, on day 21, dietary 2,500 mg/kg ADP supplementation increased the serum catalase (CAT) and liver total superoxide dismutase (T-SOD) activities (p < 0.05), whereas dietary 1,000–5,500 mg/kg ADP supplementation decreased malondialdehyde (MDA) contents in serum and liver (p < 0.05). On day 35, supplementation of 1,000 mg/kg ADP increased the serum glutathione peroxidase and CAT activities and liver T-SOD activities (p < 0.05). It decreased the MDA level of serum and liver (p < 0.05). Also, dietary 2,500 mg/kg ADP increased the villus height of jejunum and ileum on day 21 (p < 0.05), and dietary 4,000 mg/kg ADP increased the villus height of duodenum and ileum on day 35 (p < 0.05). On day 21, dietary 4,000 mg/kg ADP increased the CAT activities of the duodenum and T-SOD activities of jejunum and ileum and decreased the MDA contents in the duodenum, jejunum, and ileum (p < 0.05). On day 35, dietary inclusion of 1,000–7,000 mg/kg ADP reduced MDA contents of duodenum and jejunum (p < 0.05). Furthermore, dietary inclusion of ADP at 1,000–7,000 mg/kg decreased serum DAO activities at day 21 and day 35 (p < 0.05), and the serum D-lactic acid concentration was reduced by dietary supplementation of 1,000, 2,500, and 7,000 mg/kg ADP on day 21. In conclusion, dietary ADP exerted beneficial effects on growth performance, antioxidant capacity, and gut health in broilers; based on the studied parameters, the appropriate recommended dose is 1,000–4,000 mg/kg. These findings provided new insights into the potential application of ADP as natural growth promoters in broilers.


Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2169
Author(s):  
Dongxu Ming ◽  
Wenhui Wang ◽  
Caiyun Huang ◽  
Zijie Wang ◽  
Chenyu Shi ◽  
...  

The study objective was to assess effects of different weaning ages on growth performance, intestinal morphology and redox status in Duroc × Landrace × Large White piglets (n = 96) fed diets without antibiotic growth promoters. Piglets were selected from 24 litters based on similar body weight at 14 d of age. All piglets were allocated to two groups in a completely random design with six replicates and eight pigs per replicate (four barrows and four gilts), which were weaned at 21 (n = 48; BW = 6.87 ± 0.33 kg) and 28 (n = 48; BW = 8.49 ± 0.41 kg) days of age. After weaning, pigs were fed a corn–soybean meal-based diet. Average daily gain (ADG), average daily feed intake (ADFI), feed conversion (F:G), diarrhea incidence, gastrointestinal pH, intestinal morphology and redox status were determined. Pigs weaned at 28 d displayed increased ADG from d 8 to 14 (p < 0.01) compared with pigs weaned at 21 d. Pigs weaned at 28 d had a higher ADFI from d 0 to 7 (p < 0.01), d 8 to 14 (p < 0.01), d 15 to 28 (p < 0.05) and during the entire experimental period (p < 0.01) compared with pigs weaned at 21 d. Pigs weaned at 21 d had an improved F:G from d 15 to 28 (p < 0.05) compared with pigs weaned at 28 d. Pigs weaned at 28 d had decreased diarrhea incidence from d 8 to 14 (p < 0.01) and the entire experimental period (p < 0.01) compared with pigs weaned at 21 d. On d 28, the pH of the stomach contents in pigs weaned at 21 d was significantly higher compared with pigs weaned at 28 d (p < 0.01). On d 14, the morphology of the duodenum, jejunum and ileum in pigs weaned at 28 d was improved compared with pigs weaned at 21 d. During the experiment period, the antioxidant abilities of pigs weaned at 28 d of the heart, liver, kidney, intestinal and serum were better than pigs weaned at 21 d. In conclusion, intestinal morphology, pH of the stomach and antioxidant status of pigs weaned at 28 d were better than pigs weaned at 21 d. These factors supported better growth performance and decreased diarrhea incidence.


2021 ◽  
Vol 8 ◽  
Author(s):  
Xiyi Hu ◽  
Xianlei Li ◽  
Chuanpi Xiao ◽  
Linglian Kong ◽  
Qidong Zhu ◽  
...  

This study aimed to characterize the effects of diets with different energy levels on the growth performance, plasma parameters, and central AMPK signaling pathway in broilers under dexamethasone (DEX)-induced stress. A total of 216 1-day-old male broiler chickens were allocated to groups fed with high (HED), National Research Council-recommended (control), or low (LED) energy diets. At 10 days old, chickens were treated with or without dexamethasone (DEX, 2 mg/kg body weight) for 3 consecutive days. HED increased broiler average daily gain (ADG) at 10 days old, compared with the LED (P &lt; 0.05), while average daily feed intake (ADFI) and feed conversion rate (FCR) decreased as the dietary energy level increased (P &lt; 0.05). Chickens fed a HED had higher total protein (TP) content, albumin (ALB), glucose (GLU), total cholesterol (TCHO), high-density lipoprotein (HDL) cholesterol, and low-density lipoprotein (LDL) cholesterol, compared with the control group (P &lt; 0.05). At 13 days old, DEX decreased ADG and increased FCR in broilers fed with different energy diets (P &lt; 0.05). The DEX-HED group had a higher ADFI than non-DEX treated HED group chickens. In addition, TP, ALB, triglycerides (TG), TCHO, HDL, and LDL content levels in the DEX group were higher than those in the control group (P &lt; 0.05). The uric acid (UA) content of the LED group was higher than that of the HED group (P &lt; 0.05). Further, gene expression levels of liver kinase B1, AMP-activated protein kinase α1, neuropeptide Y, and GC receptor in the hypothalamus were increased in chickens treated with DEX (P &lt; 0.05). There was a trend toward interaction between plasma TCHO and hypothalamic LKB1 expression (0.05 &lt; P &lt; 0.1). In conclusion, this study suggests that HED improves growth performance, plasma glucose and total cholesterol at 10 days old broilers, but had no significant effect on performance, plasma parameters, and central AMPK in stressed broilers.


2019 ◽  
Vol 42 ◽  
pp. e46775
Author(s):  
Shahryar Omidi ◽  
Ardeshir Mohit ◽  
Navid Ghavi Hossein-Zadeh

An experiment was conducted to determine effects of different levels and sources of fats in diets on growth performance, carcass characteristics and immune response of turkeys during four periods (0-21, 22 to 42, 43 to 63 and 64 to 70 days of age). A completely randomized design with five treatments, six replicates and eight B.U.T.6 turkey chickens per replicate was used. Treatments included: Control diet, diet containing 2.5% of soybean oil, diet containing 2.5% fat supplement, diet containing 5% soybean oil, diet containing 5% fat supplement. The results showed that using 5% of soybean oil increased average daily gain of turkeys (89.04 g) throughout the experimental period (0-70 days) compared with the control group (81.11 g; p < 0.05). Treatments containing 2.5 and 5% soybean oil improved feed conversion ratio compared to control group (p < 0.05). Soybean oil at the levels of 2.5 and 5% was led to higher spleen and bursa percentages compared to other treatments, respectively (p < 0.05). The level and source of dietary fat had not significant effect on antibody titer against Newcastle virus vaccine on 42 and 70 days of age (p > 0.05). It can be concluded that supplementing diet with 5% soybean oil improved the performance of turkeys.


2020 ◽  
Vol 21 (3) ◽  
pp. 74-79
Author(s):  
Ahmed Elbaz ◽  
Said El-sheikh

Objective: To investigate the effect of antibiotics and/or probiotics on broiler performance, some serum metabolites, cecum microflora composition, and ileum histomorphology under the Egyptian conditions. Design: Randomized controlled experimental study. Animals: Two hundred forty 1-day-old Ross (308) chicks were reared till 35 days of age. Procedures: The birds were randomly allocated into four main groups: a control diet without additives (CON); probiotic (Lactobacillus acidophilus) supplemented diet (PRO); antibiotic (Avilamycin) supplemented diet (ANT) and a mix group (AP) that received antibiotic in the diet form 1 to 4 days of age and treated during the rest of the experimental period with probiotics. Results: Chickens fed on probiotic or antibiotic diets had linear improvement in live body weight (LBW) and feed conversion ratio (FCR) compared with the control group, while the best LBW and FCR were in the AP group. An improvement in the nutrient digestibility was observed in the probiotic added groups (PRO and AP). Serum cholesterol and low-density lipoprotein cholesterol contents decreased when antimicrobial (probiotic or antibiotic) supplementations were used, while there was an increase in high-density lipoprotein cholesterol contents, serum total protein, and albumin levels. Among all groups, cecum Clostridium perfringens and Escherichia coli counts decreased; however, there was an increase in Lactobacillus count compared to the control group. In probiotic supplemented groups (PRO and AP), a significant (P<0.05) improvement in ilea architecture. Conclusion and clinical relevance: Using probiotic after initial treatment with an antibiotic in broiler diets had a positive effect on broiler growth performance, gut health (improved cecum microbial populations and ileum histomorphology), and nutrient digestibility.


Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1581
Author(s):  
Artur Rybarczyk ◽  
Elżbieta Bogusławska-Wąs ◽  
Alicja Dłubała

The objective of the study was to determine the effects of probiotic bacteria Bacillus licheniformis and Bacillus subtilis on microbiological properties of feed mixtures and on the digestive tract content as applicable to production traits and carcass characteristics of fatteners. The experiment was performed on 83,838 fatteners from four successive (insertions) productions in two groups. From the seventy eighth day of age till marketing to the slaughter plant, the pigs were supplied with BioPlus YC probiotic (Chr. Hansen) in the amount of 400 g/t. The preparation contained a complex of probiotic bacteria Bacillus licheniformis DSM 5749, and Bacillus subtilis DSM 5750 spores in a 1:1 ratio. From the fourth insertion, after reaching a body weight of approximately 112 kg, 60 fatteners were selected from each group to measure carcass quality and half of them for meat quality evaluation. Moreover, microbiological analyses in feed and colon were performed. The study showed that BioPlus YC probiotics supplementation resulted in a significantly higher count of B. subtilis and B. licheniformis in the feed, a higher count of B. subtilis, B. licheniformis and LAB, as well as a lower count of Enterobacteriaceae, Enterococcus, Clostridium and Bacillus sp. in the mucosa and in the colorectal content of the test pigs. Our work has shown that supplementation with the BioPlus YC probiotic had a positive effect on the production traits of pigs mainly by reducing mortality (2.83%, p = 0.010), lowering feed conversion ratio—FCR (2.59 kg/kg, p = 0.013), better average daily gain—ADG (0.95 kg/day, p = 0.002) and shorter fattening period (77.25 days, p = 0.019) when compared to the control group (4.19%; 2.79 kg/kg; 0.89 kg/day; 92.8 days, respectively). The addition of the specific Bacillus bacteria did not influence carcass and meat characteristics of the test fatteners.


2013 ◽  
Vol 16 (4) ◽  
pp. 619-627 ◽  
Author(s):  
M. Pogány Simonová ◽  
A. Lauková ◽  
L. Chrastinová ◽  
I. Plachá ◽  
V. Strompfová ◽  
...  

Abstract The effect of Enterococcus faecium CCM7420 (EF) - enterocin-producing and probiotic strain of rabbit origin, Eleutherococcus senticosus extract (ES) and their combination (ES+EF) was determined on selected bacteria in faeces and caecum content, leukocytes phagocytosis, blood biochemistry and growth performance. Ninety-six weaned rabbits were divided into 3 experimental (ES, EF, ES+EF) and control group (CG). The rabbits in the groups ES and EF+ES were fed commercial diet enriched with E. senticosus extract (30 g/100 kg feed), rabbits in groups EF and CG were fed untreated diet. The rabbits in the EF and ES+EF groups were administered with an overnight culture of E. faecium CCM7420 strain (500 μl/animal/day into water, 109 CFU/ml). The treatment period lasted 21 days. The microbiological examinations in faecal samples confirmed the presence of E. faecium CCM7420 strain. In groups EF and ES+EF, the reduction of faecal coliforms, Pseudomonas-like sp., Clostridium-like sp. and S. aureus was recorded. Leucocyte phagocytosis significantly increased in all experimental groups (P<0.0001) compared to CG. The lowest GPx values were measured in the ES+EF group. Higher total protein, triglycerides and calcium concentrations were detected in experimental groups compared to CG. The cholesterol concentration decreased in the ES group. The highest average daily gain was recorded in EF group; in ES+EF the better feed conversion ratio and no mortality was recorded. These results indicated that the dietary supplementation with the E. faecium CCM7420 and E. senticosus extract stimulate the leukocytes phagocytosis and reduces the potential pathogens in rabbits digestive tract without oxidative stress and improve the growth performance.


2009 ◽  
Vol 33 (2) ◽  
pp. 84-90
Author(s):  
GALIB A.M. AL-KASSIE

This experiment was carried out at poultry house, college of Veterinary Medicine, Baghdad university,Which lasted 42 days, to by adding Thymus vulgaris the used part (Leaves, Steams and Flowers) andCinnamomum zeylanicum the used part (bark) to the concentrate diet for the experimental period for 6 weeks.The study was aimed to find out the effect of thyme or cinnamon on productive, physiological blood traits ofbroilers by using 150 unsexing Arbor Acres chicks at the age of one day. These chicks were randomly dividedinto five group equally (30 chicks each). Each group was subdivided into two equal subgroup. And fed on thefollowing ration.1-The first group was fed on ordinary diet kept as a control group.2- The second group was fed on the same ration by adding 0.5% of thyme.3- The third group was fed on the same ration by adding 1% of thyme.4- The fourth group was fed on the same ration by adding 0.5% of cinnamon.5- The fifth group was fed on the same ration by adding 1% of cinnamon.The feeding period for all groups lasted 42 days, the results revealed that There is an improving inperformance traits for all treated groups compared with control group. However, the chicks fed 0.5% thymeshould better in weekly gain period feed conversion efficiency than those fed 1% thyme. In the mean time thechicks fed 1% cinnamon should significantly higher in weekly gain and fed conversion efficiency then those fed0.5% cinnamon.However, by adding thyme or cinnamon to diet cause an improving in blood traits compared with thecontrol group. The best improving in white blood cell, were obtained in the chicks fed 1% cinnamon and theyshould significantly low better stress coefficient.


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